GREMLIN Database
TMEM220 - Transmembrane family 220, helix
PFAM: PF15071 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 106 (98)
Sequences: 2940 (2359)
Seq/√Len: 238.3
META: 0.891

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
25_A97_V3.5921.00
10_Y19_I3.4221.00
7_A10_Y2.9351.00
16_Y20_P2.7241.00
27_V31_L2.4921.00
3_L26_A2.4411.00
54_Y57_Y2.1541.00
30_L34_F2.1521.00
4_L8_V2.1421.00
6_A23_L2.0881.00
6_A19_I2.0491.00
29_C104_L1.9201.00
54_Y58_L1.6961.00
51_Y54_Y1.6601.00
15_P19_I1.6381.00
52_L55_A1.6361.00
50_V54_Y1.4891.00
63_P67_E1.4621.00
60_I64_G1.4421.00
69_S72_A1.3991.00
29_C100_V1.3951.00
48_A51_Y1.3771.00
17_L20_P1.3661.00
15_P20_P1.3551.00
71_I74_M1.3541.00
70_I73_E1.3391.00
41_P45_L1.3361.00
100_V104_L1.3221.00
61_D65_K1.3181.00
26_A30_L1.2951.00
21_I94_L1.2901.00
24_A47_L1.2581.00
4_L7_A1.2331.00
53_A56_L1.2141.00
62_W66_G1.1851.00
48_A52_L1.1751.00
64_G68_A1.1661.00
75_M78_E1.1121.00
52_L56_L1.1081.00
3_L23_L1.0841.00
70_I74_M1.0791.00
69_S73_E1.0771.00
30_L33_A1.0701.00
40_P44_Y1.0681.00
69_S74_M1.0681.00
68_A74_M1.0661.00
9_Q19_I1.0421.00
19_I23_L1.0421.00
42_L50_V1.0401.00
44_Y54_Y1.0381.00
5_F25_A1.0321.00
47_L51_Y1.0141.00
67_E71_I1.0081.00
32_A37_Y1.0001.00
65_K68_A0.9951.00
68_A72_A0.9781.00
79_K84_Y0.9741.00
23_L27_V0.9561.00
49_V52_L0.9241.00
63_P68_A0.9121.00
47_L54_Y0.9101.00
76_K79_K0.9061.00
45_L48_A0.9021.00
53_A57_Y0.8991.00
25_A101_L0.8850.99
43_L47_L0.8720.99
55_A58_L0.8680.99
75_M79_K0.8650.99
51_Y55_A0.8570.99
5_F8_V0.8450.99
52_L58_L0.8450.99
86_E89_R0.8410.99
64_G69_S0.8330.99
63_P66_G0.8310.99
66_G69_S0.8170.99
97_V101_L0.7900.99
42_L46_I0.7880.99
37_Y40_P0.7880.99
44_Y51_Y0.7860.99
41_P44_Y0.7810.99
56_L59_F0.7680.99
13_P86_E0.7670.98
42_L45_L0.7630.98
6_A10_Y0.7620.98
28_L101_L0.7580.98
41_P46_I0.7460.98
68_A71_I0.7430.98
44_Y47_L0.7400.98
63_P73_E0.7350.98
74_M78_E0.7340.98
73_E78_E0.7270.98
64_G74_M0.7190.98
48_A57_Y0.7190.98
60_I63_P0.7190.98
65_K74_M0.7170.98
66_G70_I0.7160.98
15_P87_E0.7110.98
98_A101_L0.7060.97
29_C33_A0.7050.97
66_G71_I0.7040.97
31_L36_K0.6980.97
5_F97_V0.6890.97
50_V53_A0.6870.97
95_L99_V0.6690.96
77_A83_P0.6670.96
63_P69_S0.6610.96
67_E70_I0.6540.96
45_L49_V0.6440.96
49_V56_L0.6420.96
47_L57_Y0.6350.95
5_F92_F0.6320.95
43_L46_I0.6170.94
74_M77_A0.6150.94
14_D86_E0.6120.94
2_F25_A0.6100.94
70_I75_M0.6050.94
65_K69_S0.6050.94
5_F88_A0.5970.93
48_A59_F0.5880.93
51_Y58_L0.5870.93
31_L34_F0.5850.93
62_W68_A0.5760.92
31_L37_Y0.5710.92
90_E93_G0.5610.91
2_F100_V0.5590.91
50_V57_Y0.5590.91
99_V103_I0.5520.91
55_A59_F0.5510.90
54_Y59_F0.5490.90
40_P47_L0.5470.90
65_K71_I0.5470.90
55_A95_L0.5460.90
14_D90_E0.5450.90
10_Y23_L0.5440.90
79_K83_P0.5390.89
46_I49_V0.5350.89
28_L104_L0.5350.89
100_V103_I0.5330.89
37_Y44_Y0.5330.89
49_V53_A0.5140.87
66_G74_M0.5130.87
9_Q12_D0.5070.86
83_P88_A0.5050.86
25_A100_V0.5050.86
40_P58_L0.5050.86
51_Y97_V0.5040.86
8_V13_P0.5000.86
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3j01B 1 0.8774 8 0.902 Contact Map
2losA 1 0.6132 6 0.907 Contact Map
2looA 1 0.5849 4.9 0.912 Contact Map
1wrgA 1 0.3208 2.7 0.922 Contact Map
3wmmB 1 0.2453 2.6 0.923 Contact Map
4ryoA 1 0.8396 2.5 0.924 Contact Map
2mgyA 1 0.8491 2.1 0.926 Contact Map
3w9iA 3 0.9623 2 0.927 Contact Map
4dx5A 3 0.9717 1.7 0.93 Contact Map
1s4xA 1 0.0566 1.5 0.932 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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