GREMLIN Database
DUF4476 - Domain of unknown function (DUF4476)
PFAM: PF14771 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 91 (89)
Sequences: 6013 (4910)
Seq/√Len: 520.4
META: 0.96

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
41_V65_V3.0011.00
51_E83_S2.9991.00
20_D53_N2.5281.00
28_K60_L2.1761.00
67_D70_N2.1501.00
10_L30_A1.9261.00
42_K77_A1.8561.00
3_S6_D1.7651.00
27_A57_L1.7231.00
41_V61_A1.7021.00
11_L43_Q1.6641.00
45_L58_A1.6601.00
75_Y88_N1.6281.00
7_F43_Q1.6231.00
81_S85_D1.5811.00
65_V71_Y1.5741.00
31_F57_L1.5201.00
50_F80_F1.5161.00
46_Q77_A1.4771.00
42_K46_Q1.4391.00
45_L77_A1.4281.00
38_T73_Q1.3601.00
45_L57_L1.3101.00
9_Q73_Q1.2981.00
42_K76_D1.2911.00
31_F34_N1.2871.00
20_D23_K1.2871.00
35_C66_V1.2641.00
28_K56_E1.2591.00
2_M10_L1.2491.00
11_L47_L1.2431.00
62_Y71_Y1.2371.00
49_S79_T1.2341.00
11_L15_K1.2101.00
31_F36_F1.2101.00
17_E26_V1.1981.00
4_D8_N1.1931.00
18_S49_S1.1891.00
59_K86_E1.1631.00
21_D52_S1.1551.00
15_K47_L1.1541.00
57_L61_A1.1371.00
39_A73_Q1.1371.00
48_F57_L1.1361.00
55_L59_K1.1261.00
17_E22_D1.1231.00
27_A48_F1.1151.00
17_E84_K1.1151.00
38_T67_D1.0861.00
32_K60_L1.0721.00
38_T74_L1.0671.00
37_T66_V1.0581.00
24_L28_K1.0441.00
58_A87_L1.0231.00
14_I27_A1.0201.00
60_L64_R1.0061.00
24_L53_N0.9991.00
38_T42_K0.9661.00
22_D25_S0.9521.00
55_L87_L0.9501.00
36_F41_V0.9331.00
19_F50_F0.9321.00
51_E54_R0.9151.00
43_Q46_Q0.9101.00
42_K73_Q0.9091.00
87_L90_Y0.8991.00
71_Y74_L0.8881.00
85_D89_E0.8681.00
44_L57_L0.8671.00
18_S79_T0.8341.00
31_F61_A0.8301.00
19_F80_F0.8231.00
7_F40_Q0.8151.00
83_S86_E0.8081.00
75_Y84_K0.8021.00
84_K88_N0.7921.00
74_L87_L0.7921.00
86_E89_E0.7891.00
12_Q15_K0.7841.00
56_E59_K0.7791.00
37_T40_Q0.7751.00
6_D9_Q0.7731.00
59_K90_Y0.7721.00
23_K47_L0.7641.00
80_F83_S0.7521.00
58_A78_F0.7461.00
55_L78_F0.7371.00
72_Y75_Y0.7291.00
42_K74_L0.7291.00
78_F84_K0.7201.00
39_A43_Q0.7101.00
35_C64_R0.7011.00
32_K64_R0.7011.00
58_A74_L0.6911.00
12_Q16_N0.6901.00
22_D81_S0.6881.00
7_F11_L0.6791.00
4_D43_Q0.6671.00
5_A9_Q0.6501.00
85_D88_N0.6391.00
24_L56_E0.6341.00
24_L57_L0.6321.00
27_A44_L0.6301.00
26_V30_A0.6221.00
6_D34_N0.6151.00
14_I44_L0.6111.00
55_L83_S0.6031.00
19_F22_D0.5870.99
5_A8_N0.5800.99
24_L48_F0.5780.99
14_I47_L0.5740.99
61_A65_V0.5660.99
38_T65_V0.5660.99
20_D48_F0.5660.99
75_Y87_L0.5500.99
54_R77_A0.5480.99
13_S26_V0.5470.99
81_S84_K0.5460.99
11_L44_L0.5430.99
14_I26_V0.5380.99
8_N12_Q0.5340.99
62_Y68_P0.5320.99
19_F23_K0.5310.99
9_Q12_Q0.5260.99
8_N43_Q0.5200.99
52_S82_S0.5180.99
62_Y65_V0.5170.99
2_M34_N0.5130.98
14_I23_K0.5110.98
78_F83_S0.5070.98
52_S56_E0.5060.98
61_A71_Y0.5040.98
56_E60_L0.5020.98
71_Y87_L0.5000.98
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3dtpE 2 0.967 21.8 0.835 Contact Map
1m45A 1 0.967 17.4 0.842 Contact Map
1w7jB 1 0.9341 16.5 0.844 Contact Map
4fhnB 1 0.978 16.3 0.844 Contact Map
4ociA 2 1 16 0.845 Contact Map
4mzjA 1 0.978 16 0.845 Contact Map
1wdcB 1 0.978 15.8 0.845 Contact Map
3j04B 1 0.978 15.6 0.846 Contact Map
4wpxA 1 1 15.6 0.846 Contact Map
4i2yA 1 1 15.6 0.846 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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