GREMLIN Database
DUF4446 - Protein of unknown function (DUF4446)
PFAM: PF14584 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 150 (147)
Sequences: 7028 (5236)
Seq/√Len: 431.9
META: 0.907

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
16_L21_A3.3001.00
67_R72_E2.8291.00
53_R58_E2.7241.00
144_E148_R2.6111.00
100_S114_S2.5081.00
81_K108_G2.4991.00
88_N96_D2.4651.00
102_A145_A2.3621.00
17_A20_L2.3041.00
6_L10_A2.2591.00
75_L78_A2.2401.00
128_P136_Y2.1581.00
60_A65_E2.1501.00
85_V145_A2.0101.00
13_L17_A1.9631.00
105_D109_D1.8991.00
143_K147_E1.8821.00
84_L101_L1.8621.00
23_R28_R1.8011.00
83_G145_A1.7991.00
76_D106_E1.7831.00
10_A13_L1.7811.00
109_D131_N1.7771.00
20_L24_L1.7251.00
57_L61_V1.7131.00
99_F124_V1.6971.00
63_E67_R1.6501.00
50_L54_V1.6311.00
74_R106_E1.6161.00
112_V146_I1.5731.00
79_I82_V1.5421.00
130_K135_E1.5321.00
108_G132_G1.5321.00
18_I22_V1.5251.00
3_A6_L1.4441.00
74_R80_Q1.4361.00
64_L68_L1.4251.00
42_D45_E1.4241.00
71_L75_L1.3901.00
31_Y35_L1.3771.00
87_Y100_S1.3641.00
104_L129_I1.3591.00
76_D107_N1.3551.00
16_L20_L1.3211.00
128_P135_E1.3121.00
79_I103_L1.2901.00
140_P143_K1.2801.00
22_V26_R1.2611.00
80_Q106_E1.2571.00
87_Y114_S1.2351.00
129_I146_I1.2231.00
4_I8_A1.2021.00
60_A63_E1.1831.00
129_I134_S1.1801.00
51_L54_V1.1771.00
137_E143_K1.1661.00
109_D130_K1.1561.00
77_G107_N1.1461.00
96_D135_E1.1401.00
101_L113_L1.1381.00
107_N131_N1.1261.00
15_I19_V1.1241.00
102_A112_V1.1091.00
68_L71_L1.0911.00
28_R32_R1.0891.00
92_D120_D1.0811.00
96_D136_Y1.0731.00
54_V57_L1.0681.00
21_A25_R1.0661.00
44_E48_V1.0641.00
117_H122_T1.0551.00
140_P144_E1.0441.00
67_R70_R1.0391.00
84_L126_A1.0361.00
133_K143_K1.0171.00
56_D60_A1.0101.00
48_V52_E1.0071.00
103_L111_V1.0041.00
10_A17_A0.9991.00
49_S53_R0.9811.00
58_E62_D0.9751.00
144_E147_E0.9691.00
79_I111_V0.9691.00
92_D119_R0.9641.00
45_E49_S0.9561.00
25_R29_R0.9451.00
118_G121_G0.9351.00
49_S52_E0.9331.00
83_G148_R0.9091.00
81_K106_E0.8921.00
19_V23_R0.8861.00
104_L132_G0.8841.00
13_L20_L0.8781.00
74_R78_A0.8701.00
85_V144_E0.8671.00
118_G122_T0.8671.00
22_V25_R0.8631.00
99_F126_A0.8611.00
77_G80_Q0.8531.00
89_A116_I0.8481.00
101_L111_V0.8471.00
61_V64_L0.8451.00
59_E63_E0.8431.00
48_V51_L0.8391.00
70_R74_R0.8381.00
63_E66_A0.8371.00
104_L146_I0.8371.00
47_L51_L0.8261.00
24_L27_L0.8201.00
62_D65_E0.8161.00
52_E55_D0.8141.00
145_A149_A0.8111.00
134_S138_L0.8101.00
89_A121_G0.8081.00
62_D66_A0.8071.00
39_G42_D0.8041.00
40_G43_L0.8041.00
17_A21_A0.7991.00
55_D59_E0.7961.00
86_R136_Y0.7941.00
55_D58_E0.7931.00
83_G149_A0.7911.00
72_E76_D0.7901.00
89_A97_Q0.7861.00
88_N95_G0.7831.00
51_L55_D0.7831.00
3_A10_A0.7801.00
65_E69_A0.7701.00
66_A70_R0.7691.00
59_E62_D0.7661.00
11_L15_I0.7511.00
89_A96_D0.7481.00
37_G44_E0.7451.00
73_R76_D0.7331.00
9_L13_L0.7311.00
6_L13_L0.7281.00
31_Y34_L0.7261.00
56_D59_E0.7251.00
12_V15_I0.7201.00
129_I138_L0.7131.00
104_L108_G0.7121.00
38_K45_E0.7091.00
27_L31_Y0.7071.00
66_A69_A0.7011.00
42_D46_V0.7011.00
113_L126_A0.6971.00
46_V50_L0.6901.00
138_L146_I0.6851.00
19_V22_V0.6821.00
134_S146_I0.6801.00
130_K133_K0.6781.00
21_A24_L0.6681.00
53_R56_D0.6681.00
58_E61_V0.6540.99
46_V51_L0.6430.99
43_L47_L0.6370.99
41_K45_E0.6350.99
93_T117_H0.6330.99
47_L50_L0.6330.99
24_L28_R0.6330.99
7_A11_L0.6320.99
114_S139_S0.6310.99
50_L53_R0.6260.99
38_K42_D0.6210.99
89_A98_S0.6140.99
45_E48_V0.6120.99
115_S126_A0.6120.99
37_G42_D0.6120.99
82_V109_D0.6120.99
120_D124_V0.6080.99
52_E56_D0.6070.99
36_G55_D0.6040.99
105_D132_G0.6010.99
49_S55_D0.5990.99
26_R30_R0.5980.99
27_L30_R0.5980.99
5_A8_A0.5960.99
9_L14_L0.5920.99
99_F117_H0.5900.99
35_L46_V0.5890.99
8_A12_V0.5890.99
115_S122_T0.5880.99
7_A10_A0.5830.99
57_L60_A0.5820.99
35_L38_K0.5820.99
24_L31_Y0.5810.99
18_I25_R0.5800.99
128_P134_S0.5740.99
117_H124_V0.5730.99
129_I132_G0.5700.99
85_V100_S0.5680.99
40_G44_E0.5660.98
6_L9_L0.5630.98
10_A14_L0.5620.98
91_E94_G0.5620.98
14_L17_A0.5550.98
79_I105_D0.5550.98
92_D118_G0.5450.98
34_L46_V0.5440.98
102_A149_A0.5420.98
90_F118_G0.5380.98
53_R57_L0.5380.98
8_A11_L0.5330.98
30_R48_V0.5310.98
11_L14_L0.5310.98
126_A136_Y0.5300.98
86_R96_D0.5270.98
90_F99_F0.5190.97
75_L79_I0.5190.97
97_Q121_G0.5160.97
37_G43_L0.5150.97
35_L97_Q0.5120.97
43_L46_V0.5110.97
34_L40_G0.5100.97
51_L57_L0.5100.97
70_R73_R0.5080.97
84_L111_V0.5060.97
112_V127_K0.5060.97
85_V148_R0.5030.97
35_L89_A0.5000.97
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4cq4A 4 0.5333 68.5 0.907 Contact Map
4i5sA 2 0.7467 57.7 0.913 Contact Map
3pjsK 4 0.46 56.5 0.914 Contact Map
3effK 4 0.4333 52.7 0.916 Contact Map
4cbcA 3 0.1667 48 0.918 Contact Map
3rvyA 3 0.1667 44.8 0.92 Contact Map
4gycB 2 0.14 44.3 0.92 Contact Map
2wujA 2 0.3333 37 0.923 Contact Map
4ltoA 3 0.46 35.4 0.924 Contact Map
3c9iA 5 0.9467 33.6 0.925 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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