GREMLIN Database
Prok-E2_D - Prokaryotic E2 family D
PFAM: PF14460 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 169 (156)
Sequences: 1752 (1277)
Seq/√Len: 102.2
META: 0.824

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
39_P62_G5.5621.00
60_H94_F4.7261.00
62_G77_L3.2841.00
41_K57_R3.0821.00
64_V77_L2.9671.00
124_E128_N2.8471.00
37_T65_F2.8431.00
72_L103_V2.7471.00
34_V66_K2.3541.00
161_F165_D2.3081.00
151_L155_L2.2721.00
20_I85_P2.1301.00
44_M60_H2.0281.00
37_T42_R1.9881.00
43_P57_R1.9821.00
148_L152_L1.9791.00
22_P25_V1.9031.00
98_Y102_R1.8261.00
60_H127_F1.8131.00
141_G165_D1.8021.00
152_L157_K1.7411.00
63_L74_V1.7401.00
65_F74_V1.7031.00
79_G163_V1.5951.00
66_K73_Y1.5901.00
35_W123_E1.5871.00
108_V130_E1.5291.00
71_S102_R1.5271.00
78_K90_Y1.5251.00
149_W155_L1.5161.00
51_L56_G1.4951.00
139_H147_D1.4701.00
113_S118_S1.4471.00
67_A122_W1.4321.00
108_V125_A1.4251.00
67_A72_L1.4021.00
75_F101_G1.3931.00
26_L64_V1.3831.00
92_A131_F1.3490.99
149_W152_L1.3410.99
62_G80_N1.3330.99
74_V126_F1.2900.99
26_L83_P1.2850.99
94_F126_F1.2820.99
65_F127_F1.2730.99
23_E83_P1.2600.99
151_L157_K1.2540.99
37_T123_E1.2510.99
63_L92_A1.2400.99
25_V119_I1.2370.99
139_H143_F1.2300.99
151_L154_G1.2270.99
126_F129_S1.2140.99
68_S73_Y1.2130.99
153_A156_K1.1930.99
103_V122_W1.1900.99
160_P164_E1.1790.98
103_V126_F1.1720.98
66_K75_F1.1630.98
39_P80_N1.1350.98
149_W153_A1.1310.98
162_P166_L1.1300.98
65_F126_F1.1260.98
42_R123_E1.0990.98
47_R132_T1.0980.98
95_F134_P1.0960.98
78_K163_V1.0890.97
97_V103_V1.0790.97
36_W83_P1.0770.97
78_K164_E1.0740.97
88_P168_P1.0700.97
41_K59_P1.0640.97
19_G27_A1.0540.97
141_G144_N1.0430.97
139_H151_L1.0300.96
62_G163_V1.0260.96
42_R60_H1.0170.96
145_P149_W0.9970.96
140_K143_F0.9810.95
83_P87_T0.9700.95
19_G28_R0.9580.95
59_P160_P0.9470.94
122_W131_F0.9440.94
110_V121_A0.9410.94
145_P152_L0.9370.94
75_F89_L0.9330.94
50_A53_I0.9260.94
37_T127_F0.9260.94
33_L119_I0.9190.93
136_L140_K0.9010.93
20_I27_A0.9000.93
140_K148_L0.8950.92
97_V126_F0.8950.92
139_H142_G0.8910.92
148_L153_A0.8900.92
115_S118_S0.8810.92
114_A118_S0.8810.92
62_G79_G0.8790.92
120_E124_E0.8780.92
110_V127_F0.8760.92
96_N104_C0.8740.91
18_S27_A0.8730.91
154_G157_K0.8690.91
39_P79_G0.8690.91
36_W62_G0.8630.91
151_L161_F0.8580.91
18_S21_L0.8530.90
140_K144_N0.8510.90
142_G145_P0.8500.90
36_W64_V0.8460.90
63_L127_F0.8440.90
42_R127_F0.8430.90
17_P27_A0.8300.89
26_L82_R0.8290.89
141_G145_P0.8290.89
126_F131_F0.8150.88
90_Y167_V0.8130.88
147_D150_K0.8030.88
98_Y136_L0.8030.88
21_L25_V0.8010.87
64_V90_Y0.7980.87
63_L76_A0.7960.87
78_K81_R0.7930.87
105_L110_V0.7910.87
27_A32_L0.7710.85
132_T135_N0.7600.85
83_P133_H0.7570.84
97_V135_N0.7530.84
48_D53_I0.7520.84
136_L139_H0.7500.84
142_G148_L0.7470.84
37_T40_Q0.7450.83
79_G164_E0.7350.83
107_S130_E0.7340.82
35_W133_H0.7330.82
73_Y102_R0.7270.82
146_V151_L0.7210.81
35_W161_F0.7180.81
47_R53_I0.7160.81
113_S121_A0.7120.81
109_R160_P0.7100.80
77_L87_T0.7050.80
127_F161_F0.6970.79
133_H149_W0.6960.79
23_E82_R0.6890.78
146_V150_K0.6780.77
76_A92_A0.6780.77
148_L156_K0.6750.77
25_V33_L0.6740.77
20_I32_L0.6720.77
50_A54_N0.6660.76
72_L105_L0.6650.76
84_T102_R0.6640.76
60_H65_F0.6590.75
29_G107_S0.6580.75
146_V149_W0.6580.75
56_G95_F0.6570.75
141_G148_L0.6570.75
43_P128_N0.6560.75
28_R31_E0.6510.74
144_N148_L0.6510.74
19_G29_G0.6490.74
46_F133_H0.6480.74
18_S59_P0.6480.74
77_L83_P0.6480.74
20_I30_G0.6430.73
68_S71_S0.6390.73
23_E27_A0.6320.72
103_V131_F0.6300.72
159_K164_E0.6280.72
152_L155_L0.6260.71
144_N147_D0.6230.71
84_T87_T0.6210.71
141_G161_F0.6170.70
148_L155_L0.6090.69
105_L108_V0.6070.69
129_S162_P0.6030.69
55_G95_F0.5990.68
67_A105_L0.5980.68
78_K88_P0.5930.67
98_Y135_N0.5930.67
91_H101_G0.5920.67
76_A111_P0.5900.67
143_F165_D0.5880.67
38_P111_P0.5880.67
51_L95_F0.5870.67
150_K155_L0.5860.66
137_T142_G0.5830.66
46_F55_G0.5820.66
94_F97_V0.5820.66
54_N154_G0.5820.66
46_F95_F0.5800.66
108_V129_S0.5760.65
62_G78_K0.5750.65
92_A133_H0.5700.64
23_E26_L0.5690.64
57_R115_S0.5660.64
122_W152_L0.5600.63
52_G150_K0.5590.63
148_L162_P0.5550.62
44_M108_V0.5550.62
110_V125_A0.5540.62
64_V89_L0.5530.62
44_M103_V0.5510.62
143_F161_F0.5490.61
51_L54_N0.5480.61
56_G94_F0.5480.61
120_E123_E0.5440.61
24_N86_D0.5420.60
13_G16_V0.5400.60
92_A122_W0.5380.60
70_G97_V0.5360.60
45_F72_L0.5350.59
62_G116_V0.5330.59
61_P89_L0.5310.59
22_P119_I0.5290.59
105_L125_A0.5270.58
41_K164_E0.5250.58
70_G78_K0.5240.58
138_R145_P0.5210.57
146_V153_A0.5200.57
46_F87_T0.5170.57
63_L89_L0.5150.57
24_N33_L0.5140.56
39_P59_P0.5140.56
67_A113_S0.5130.56
161_F166_L0.5110.56
151_L165_D0.5090.56
13_G69_G0.5090.56
21_L115_S0.5080.56
101_G126_F0.5050.55
138_R150_K0.5030.55
65_F122_W0.5030.55
80_N160_P0.5000.54
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2hlwA 1 0.7515 11.6 0.938 Contact Map
2e2cA 2 0.8047 11.6 0.938 Contact Map
4jquA 1 0.8284 10.8 0.939 Contact Map
1i7kA 1 0.8047 10.8 0.939 Contact Map
2a4dA 1 0.7456 10.6 0.94 Contact Map
4onlA 1 0.7515 10.3 0.94 Contact Map
4gprA 1 0.8284 9.4 0.941 Contact Map
4ds2A 1 0.8107 8.4 0.942 Contact Map
3rz3A 1 0.8402 8.2 0.943 Contact Map
1zdnA 2 0.8166 8.2 0.943 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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