GREMLIN Database
Transglut_i_TM - Inactive transglutaminase fused to 7 transmembrane helices
PFAM: PF14400 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 161 (159)
Sequences: 1475 (1068)
Seq/√Len: 84.7
META: 0.765

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
61_L72_A5.2241.00
35_K133_D4.8281.00
52_E70_R3.5701.00
37_S71_R3.4881.00
59_Y83_Q3.4851.00
2_K8_F3.3181.00
127_A141_L2.9881.00
75_S136_S2.9001.00
77_R131_S2.6851.00
29_A34_V2.6371.00
24_R84_T2.4181.00
114_E118_T2.2861.00
124_L137_F2.2361.00
60_G77_R2.2181.00
76_I79_A2.1581.00
35_K75_S2.1021.00
29_A79_A2.0601.00
138_T142_I2.0111.00
2_K6_L1.9511.00
112_L120_A1.8671.00
25_V38_L1.8591.00
27_F59_Y1.7981.00
56_S74_W1.7881.00
46_G94_D1.7681.00
113_E116_Y1.7411.00
49_I91_V1.6671.00
140_E143_K1.6591.00
127_A154_V1.6511.00
60_G131_S1.6421.00
1_Y5_V1.5201.00
1_Y6_L1.5141.00
26_E84_T1.5080.99
133_D136_S1.5030.99
25_V36_V1.4740.99
123_L154_V1.4600.99
27_F83_Q1.4590.99
137_F155_A1.4030.99
28_E82_P1.3980.99
20_T90_Q1.3090.99
115_P118_T1.3040.99
29_A59_Y1.3030.98
9_P60_G1.2970.98
2_K10_L1.2720.98
147_D150_P1.2250.98
56_S61_L1.2170.98
121_E125_D1.2120.98
79_A83_Q1.2090.98
10_L92_Y1.1930.97
60_G132_A1.1920.97
60_G136_S1.1910.97
131_S136_S1.1740.97
145_L154_V1.1720.97
52_E87_Y1.1700.97
64_E73_E1.1650.97
125_D129_E1.1530.97
130_R144_R1.1390.96
61_L76_I1.1160.96
130_R140_E1.0950.96
78_R128_R1.0950.96
20_T50_L1.0570.95
77_R136_S1.0530.95
35_K132_A1.0480.94
48_T51_D1.0350.94
114_E117_A1.0260.94
117_A121_E1.0170.93
19_W22_E1.0000.93
27_F81_G0.9890.92
122_A153_N0.9850.92
55_A152_Q0.9760.92
49_I89_A0.9680.92
11_L14_E0.9560.91
27_F34_V0.9540.91
17_E93_R0.9520.91
52_E74_W0.9510.91
135_E138_T0.9360.90
75_S140_E0.9280.90
13_G21_V0.9280.90
153_N156_L0.9280.90
9_P77_R0.9240.90
8_F92_Y0.9210.90
33_P157_L0.9130.89
22_E27_F0.9040.89
140_E156_L0.8990.88
134_P138_T0.8980.88
9_P85_L0.8980.88
145_L150_P0.8960.88
25_V71_R0.8940.88
66_E69_N0.8900.88
62_S75_S0.8720.87
29_A81_G0.8710.87
39_A71_R0.8680.87
131_S137_F0.8680.87
72_A86_Y0.8630.86
119_A156_L0.8610.86
145_L153_N0.8600.86
150_P158_L0.8500.85
80_S104_P0.8490.85
46_G75_S0.8480.85
10_L54_F0.8460.85
100_P107_P0.8430.85
91_V102_P0.8370.84
40_L88_R0.8230.83
137_F141_L0.8200.83
21_V101_P0.8180.83
126_E154_V0.8160.83
25_V158_L0.8110.83
23_A83_Q0.8110.83
51_D54_F0.8080.82
72_A85_L0.8060.82
3_H10_L0.8050.82
127_A137_F0.8040.82
103_P109_P0.7960.81
126_E130_R0.7940.81
132_A136_S0.7910.81
78_R106_E0.7860.81
78_R99_P0.7850.80
126_E141_L0.7790.80
45_P119_A0.7760.80
2_K92_Y0.7710.79
120_A157_L0.7700.79
107_P117_A0.7680.79
145_L156_L0.7640.79
53_N124_L0.7540.78
74_W153_N0.7530.78
77_R132_A0.7470.77
39_A44_Q0.7450.77
54_F110_P0.7420.77
21_V91_V0.7390.76
9_P131_S0.7390.76
47_F93_R0.7380.76
54_F61_L0.7360.76
105_P134_P0.7330.76
56_S60_G0.7320.76
43_L101_P0.7310.76
59_Y139_R0.7300.75
137_F157_L0.7300.75
148_P155_A0.7260.75
75_S130_R0.7260.75
84_T88_R0.7230.75
87_Y131_S0.7210.75
59_Y79_A0.7090.73
44_Q90_Q0.7090.73
126_E144_R0.7020.73
111_E117_A0.7020.73
2_K54_F0.7020.73
95_P102_P0.7020.73
36_V86_Y0.6960.72
8_F22_E0.6950.72
14_E99_P0.6920.72
40_L72_A0.6860.71
29_A33_P0.6850.71
61_L132_A0.6840.71
2_K141_L0.6840.71
41_P70_R0.6820.70
62_S86_Y0.6780.70
128_R137_F0.6760.70
6_L53_N0.6710.69
42_S49_I0.6690.69
9_P56_S0.6680.69
133_D145_L0.6660.69
4_L36_V0.6650.69
54_F63_I0.6620.68
33_P51_D0.6540.67
56_S85_L0.6530.67
54_F70_R0.6500.67
109_P117_A0.6490.67
144_R150_P0.6490.67
6_L10_L0.6460.66
72_A132_A0.6430.66
63_I135_E0.6400.66
41_P56_S0.6380.65
58_G82_P0.6370.65
29_A157_L0.6330.65
1_Y123_L0.6240.64
142_I146_N0.6160.63
37_S73_E0.6150.62
11_L58_G0.6140.62
62_S73_E0.6140.62
116_Y156_L0.6130.62
3_H59_Y0.6120.62
47_F59_Y0.6120.62
40_L70_R0.6100.62
35_K73_E0.6100.62
20_T23_A0.6090.62
39_A69_N0.6060.61
100_P103_P0.6060.61
95_P139_R0.6060.61
47_F76_I0.6050.61
29_A32_G0.6030.61
82_P96_S0.6010.61
10_L40_L0.5920.60
57_P70_R0.5890.59
40_L51_D0.5860.59
52_E89_A0.5850.59
31_G78_R0.5840.59
61_L152_Q0.5830.58
113_E156_L0.5770.58
18_V46_G0.5740.57
63_I72_A0.5730.57
55_A74_W0.5720.57
78_R108_E0.5700.57
52_E105_P0.5690.57
74_W146_N0.5650.56
113_E118_T0.5620.56
38_L59_Y0.5610.56
13_G25_V0.5580.55
154_V159_S0.5580.55
45_P107_P0.5570.55
54_F111_E0.5560.55
24_R53_N0.5540.55
39_A91_V0.5540.55
138_T159_S0.5540.55
36_V39_A0.5540.55
128_R134_P0.5530.54
1_Y98_E0.5470.54
73_E92_Y0.5410.53
146_N150_P0.5380.52
8_F51_D0.5360.52
96_S148_P0.5360.52
5_V25_V0.5350.52
59_Y98_E0.5340.52
12_P87_Y0.5340.52
40_L137_F0.5340.52
54_F152_Q0.5330.52
107_P110_P0.5310.52
8_F49_I0.5300.51
97_A125_D0.5260.51
51_D90_Q0.5180.50
55_A151_D0.5170.50
52_E134_P0.5140.49
8_F35_K0.5110.49
13_G28_E0.5100.49
139_R156_L0.5090.49
134_P139_R0.5080.48
6_L54_F0.5040.48
22_E90_Q0.5020.48
139_R157_L0.5000.47
6_L93_R0.5000.47
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4gmoA 1 0.3168 16.6 0.939 Contact Map
4ot9A 2 0.2671 12.9 0.942 Contact Map
2dvyA 4 0.6708 10 0.945 Contact Map
1vccA 1 0.4348 6.8 0.949 Contact Map
2wacA 1 0.441 6.7 0.949 Contact Map
4d0pA 1 0.4658 6.5 0.95 Contact Map
2zetC 1 0.3478 4.6 0.953 Contact Map
4ziqA 2 0.7764 4.2 0.954 Contact Map
2ygwA 2 0.4907 4.1 0.954 Contact Map
2vglM 1 0.4969 4 0.954 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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