GREMLIN Database
DUF4422 - Domain of unknown function (DUF4422)
PFAM: PF14393 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 229 (224)
Sequences: 3377 (2936)
Seq/√Len: 196.2
META: 0.804

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
47_K197_E4.3521.00
21_I56_A3.1801.00
146_I161_D3.1561.00
25_A37_G3.1131.00
51_Y194_F3.0681.00
151_E199_R2.8731.00
195_E198_K2.5991.00
208_Y215_F2.5881.00
152_K195_E2.5611.00
26_A39_N2.5411.00
150_K157_L2.3301.00
3_Y64_L2.3041.00
188_W220_L2.2921.00
159_A227_K2.2281.00
144_K147_E2.2271.00
23_V56_A2.1801.00
57_L186_C2.1401.00
153_Y191_P2.1361.00
152_K191_P2.1061.00
144_K199_R2.1031.00
79_F174_M2.0461.00
3_Y60_A1.9931.00
142_L164_M1.9801.00
54_L189_L1.9041.00
148_I196_L1.8971.00
22_Q28_K1.8661.00
184_E224_W1.8631.00
146_I160_F1.8571.00
130_Y143_D1.8541.00
57_L182_F1.8191.00
145_A216_L1.8101.00
73_C78_Y1.7991.00
115_D178_K1.7661.00
57_L72_L1.7601.00
104_K108_E1.7431.00
187_E191_P1.7161.00
146_I157_L1.7031.00
157_L161_D1.7011.00
134_D139_I1.6911.00
195_E199_R1.6501.00
36_L40_T1.6461.00
159_A226_E1.6451.00
148_I152_K1.6261.00
118_L225_F1.5651.00
188_W192_I1.5501.00
37_G40_T1.5311.00
144_K196_L1.5191.00
185_Y189_L1.5131.00
177_M181_L1.5011.00
178_K181_L1.4981.00
148_I195_E1.4831.00
60_A70_I1.4821.00
64_L67_A1.4761.00
139_I143_D1.4691.00
200_I212_V1.4091.00
60_A72_L1.4081.00
197_E213_Y1.3931.00
40_T59_W1.3631.00
129_I169_F1.3521.00
22_Q25_A1.3391.00
163_V222_N1.3361.00
6_T35_Y1.3241.00
103_T106_E1.3141.00
196_L216_L1.3041.00
130_Y134_D1.3031.00
170_Y222_N1.3021.00
148_I199_R1.2921.00
183_D187_E1.2871.00
60_A64_L1.2791.00
179_K183_D1.2701.00
50_Y202_F1.2691.00
5_A56_A1.2621.00
49_P210_K1.2131.00
141_D205_Y1.2051.00
22_Q37_G1.2001.00
145_A196_L1.1981.00
41_G59_W1.1941.00
70_I179_K1.1911.00
129_I171_P1.1891.00
160_F223_V1.1891.00
74_H172_F1.1791.00
26_A46_E1.1641.00
73_C79_F1.1591.00
138_G141_D1.1481.00
159_A163_V1.1401.00
156_Y227_K1.1401.00
207_G210_K1.1341.00
160_F164_M1.1111.00
66_D179_K1.1101.00
206_D209_Q1.0991.00
61_W186_C1.0951.00
130_Y165_N1.0941.00
5_A72_L1.0941.00
147_E199_R1.0911.00
149_I160_F1.0751.00
194_F198_K1.0621.00
162_K226_E1.0551.00
42_D62_K1.0501.00
81_F106_E1.0481.00
132_H219_R1.0341.00
21_I36_L1.0341.00
137_H219_R1.0331.00
163_V226_E1.0291.00
59_W63_N1.0281.00
177_M182_F1.0261.00
109_K112_K1.0251.00
155_E227_K1.0211.00
196_L200_I1.0201.00
140_E143_D1.0081.00
137_H209_Q1.0041.00
181_L184_E1.0001.00
133_Y137_H0.9991.00
51_Y197_E0.9941.00
38_D45_S0.9941.00
124_Y127_E0.9931.00
194_F197_E0.9911.00
47_K51_Y0.9911.00
156_Y159_A0.9911.00
19_I36_L0.9891.00
64_L179_K0.9810.99
6_T12_M0.9800.99
3_Y21_I0.9760.99
133_Y212_V0.9750.99
158_E162_K0.9750.99
32_D37_G0.9650.99
54_L217_A0.9640.99
61_W187_E0.9630.99
28_K31_E0.9610.99
221_L225_F0.9570.99
128_T131_S0.9480.99
145_A148_I0.9450.99
132_H171_P0.9450.99
137_H208_Y0.9450.99
137_H215_F0.9430.99
22_Q26_A0.9370.99
25_A28_K0.9360.99
50_Y210_K0.9360.99
146_I150_K0.9350.99
184_E187_E0.9350.99
215_F219_R0.9310.99
70_I182_F0.9280.99
106_E109_K0.9280.99
166_G226_E0.9200.99
74_H77_R0.9130.99
22_Q32_D0.9080.99
116_I225_F0.9030.99
67_A179_K0.9000.99
154_P157_L0.8960.99
138_G209_Q0.8930.99
4_V18_Y0.8810.99
180_E184_E0.8770.99
22_Q33_L0.8720.99
9_P35_Y0.8700.99
171_P219_R0.8680.99
157_L160_F0.8660.99
191_P194_F0.8630.99
72_L175_F0.8630.99
43_N46_E0.8520.99
70_I183_D0.8510.99
182_F186_C0.8480.99
129_I219_R0.8460.98
6_T10_Y0.8400.98
127_E135_H0.8380.98
151_E195_E0.8370.98
157_L163_V0.8340.98
133_Y141_D0.8340.98
25_A45_S0.8330.98
57_L175_F0.8310.98
118_L221_L0.8310.98
159_A162_K0.8300.98
147_E150_K0.8210.98
142_L145_A0.8210.98
121_K168_S0.8190.98
107_I111_L0.8150.98
48_N75_Y0.8150.98
97_K100_K0.8140.98
156_Y188_W0.8080.98
61_W183_D0.8070.98
16_D104_K0.8020.98
130_Y146_I0.7960.98
40_T63_N0.7870.98
175_F182_F0.7710.97
6_T13_P0.7700.97
129_I132_H0.7700.97
116_I177_M0.7670.97
22_Q27_L0.7670.97
123_N167_N0.7660.97
67_A70_I0.7650.97
11_E98_K0.7650.97
130_Y139_I0.7650.97
133_Y219_R0.7640.97
18_Y73_C0.7620.97
149_I157_L0.7590.97
133_Y138_G0.7570.97
110_L114_Y0.7550.97
92_I96_N0.7520.97
93_D96_N0.7510.97
3_Y19_I0.7370.96
130_Y142_L0.7360.96
114_Y117_I0.7280.96
50_Y213_Y0.7260.96
202_F213_Y0.7250.96
138_G205_Y0.7230.96
107_I176_I0.7210.96
149_I192_I0.7200.96
158_E161_D0.7190.96
53_E74_H0.7190.96
48_N55_T0.7180.96
102_L107_I0.7170.96
141_D209_Q0.7120.96
186_C190_F0.7100.96
106_E110_L0.7070.95
52_C215_F0.7060.95
41_G63_N0.7050.95
115_D118_L0.7040.95
147_E151_E0.7030.95
202_F212_V0.7020.95
202_F210_K0.6980.95
47_K194_F0.6940.95
153_Y156_Y0.6930.95
47_K50_Y0.6920.95
93_D97_K0.6910.95
45_S48_N0.6870.95
141_D212_V0.6830.95
169_F222_N0.6820.94
39_N43_N0.6810.94
123_N168_S0.6780.94
98_K101_I0.6770.94
145_A220_L0.6720.94
212_V215_F0.6710.94
64_L70_I0.6660.94
25_A39_N0.6640.94
38_D48_N0.6590.93
95_N99_K0.6590.93
15_D104_K0.6580.93
144_K200_I0.6570.93
42_D58_Y0.6520.93
92_I99_K0.6510.93
143_D146_I0.6510.93
5_A21_I0.6470.93
185_Y221_L0.6460.93
193_L217_A0.6430.93
28_K32_D0.6410.92
39_N45_S0.6400.92
118_L121_K0.6320.92
115_D119_P0.6310.92
5_A23_V0.6300.92
201_D205_Y0.6300.92
128_T167_N0.6260.92
74_H174_M0.6210.91
46_E49_P0.6170.91
156_Y223_V0.6140.91
53_E173_N0.6140.91
3_Y70_I0.6130.91
159_A164_M0.6130.91
127_E131_S0.6130.91
52_C214_G0.6130.91
200_I213_Y0.6130.91
17_I104_K0.6120.91
92_I95_N0.6110.91
10_Y13_P0.6100.90
105_E109_K0.6060.90
4_V12_M0.6060.90
77_R172_F0.6040.90
201_D204_N0.6030.90
13_P19_I0.6020.90
20_P23_V0.6020.90
87_K90_N0.5990.90
163_V223_V0.5990.90
211_R214_G0.5980.90
117_I176_I0.5980.90
107_I110_L0.5980.90
25_A32_D0.5900.89
220_L223_V0.5870.89
50_Y197_E0.5860.89
129_I166_G0.5860.89
7_H23_V0.5850.89
114_Y119_P0.5840.89
54_L174_M0.5810.88
144_K148_I0.5790.88
7_H75_Y0.5790.88
192_I220_L0.5770.88
52_C173_N0.5740.88
61_W190_F0.5700.87
137_H216_L0.5670.87
145_A160_F0.5650.87
161_D164_M0.5630.87
132_H169_F0.5590.86
44_I63_N0.5580.86
72_L182_F0.5580.86
173_N214_G0.5580.86
196_L213_Y0.5570.86
119_P122_R0.5550.86
44_I55_T0.5550.86
111_L114_Y0.5530.86
60_A182_F0.5520.86
205_Y208_Y0.5500.85
162_K166_G0.5490.85
91_K97_K0.5490.85
51_Y213_Y0.5450.85
150_K153_Y0.5440.85
38_D75_Y0.5440.85
17_I107_I0.5440.85
222_N225_F0.5410.84
13_P20_P0.5410.84
91_K95_N0.5410.84
174_M214_G0.5390.84
92_I97_K0.5390.84
209_Q219_R0.5380.84
14_K20_P0.5370.84
153_Y188_W0.5370.84
131_S134_D0.5340.84
146_I223_V0.5290.83
75_Y211_R0.5260.83
96_N99_K0.5240.83
211_R218_E0.5240.83
222_N227_K0.5230.82
131_S135_H0.5200.82
3_Y67_A0.5190.82
189_L193_L0.5180.82
53_E174_M0.5160.82
97_K101_I0.5160.82
96_N100_K0.5150.81
191_P195_E0.5120.81
122_R171_P0.5110.81
216_L219_R0.5110.81
175_F221_L0.5100.81
192_I196_L0.5080.81
22_Q31_E0.5070.80
110_L117_I0.5070.80
100_K104_K0.5060.80
143_D147_E0.5060.80
42_D46_E0.5050.80
163_V169_F0.5010.80
170_Y221_L0.5000.80
124_Y132_H0.5000.80
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4besA 2 0.4279 3.6 0.969 Contact Map
4hbdA 1 0.3362 3.2 0.969 Contact Map
2f8yA 1 0.6201 2.6 0.971 Contact Map
4tumA 1 0.4629 2.5 0.971 Contact Map
1iknD 1 0.5328 2.4 0.971 Contact Map
2zgdA 2 0.3013 2.4 0.971 Contact Map
2nwuA 2 0.3668 2.3 0.972 Contact Map
2arhA 3 0.4629 2.1 0.972 Contact Map
2fpqA 1 0.2183 2.1 0.972 Contact Map
2jucA 1 0.2402 2 0.972 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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