GREMLIN Database
DUF4421 - Domain of unknown function (DUF4421)
PFAM: PF14391 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 301 (299)
Sequences: 2444 (2146)
Seq/√Len: 124.1
META: 0.881

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
159_F203_G5.4991.00
135_F161_L5.3811.00
157_G205_Y5.2701.00
94_Y128_G4.4401.00
157_G207_Y4.2461.00
268_G296_Y4.1271.00
216_F259_G3.4781.00
146_F218_N3.4041.00
75_T78_L3.3271.00
206_A218_N3.2181.00
155_S207_Y2.9661.00
8_E153_K2.7531.00
53_S58_S2.5771.00
266_F298_G2.4491.00
140_F206_A2.3761.00
208_N216_F2.3761.00
119_R122_L2.3321.00
51_G60_G2.3211.00
75_T83_N2.2371.00
78_L83_N2.2331.00
75_T79_D2.2161.00
92_D255_R2.1551.00
136_N156_A2.0451.00
95_Y125_R2.0411.00
16_V52_F2.0301.00
89_W131_A2.0001.00
79_D83_N1.9811.00
167_Y197_F1.9271.00
73_G77_Y1.9221.00
73_G78_L1.9211.00
135_F159_F1.9151.00
90_G132_Y1.9101.00
75_T81_Q1.9011.00
119_R172_A1.8781.00
91_I131_A1.8771.00
164_S200_G1.8751.00
209_W219_A1.8751.00
143_R150_E1.8591.00
88_K137_H1.8431.00
10_Y151_I1.8131.00
119_R174_S1.7851.00
264_R299_Y1.7741.00
107_G110_D1.7461.00
152_Q208_N1.7401.00
219_A256_L1.7371.00
78_L82_L1.7201.00
87_R150_E1.7001.00
211_F215_W1.6831.00
155_S210_V1.6821.00
105_D109_D1.6731.00
42_P98_Y1.6501.00
75_T80_F1.6491.00
53_S149_S1.6431.00
158_S205_Y1.6351.00
211_F214_N1.6221.00
105_D108_D1.6151.00
177_L181_L1.6041.00
93_L129_L1.6021.00
151_I214_N1.5941.00
157_G209_W1.5861.00
145_A257_A1.5501.00
19_Y62_S1.5221.00
17_R51_G1.5211.00
73_G79_D1.5171.00
77_Y81_Q1.5161.00
106_F109_D1.4931.00
134_I160_L1.4801.00
223_L228_G1.4621.00
208_N218_N1.4601.00
17_R266_F1.4471.00
77_Y83_N1.4411.00
79_D98_Y1.4351.00
133_Y137_H1.4341.00
162_G202_G1.4281.00
217_L258_L1.4281.00
259_G262_S1.4141.00
89_W133_Y1.4121.00
19_Y49_G1.4071.00
65_P68_L1.3661.00
24_Y291_G1.3531.00
146_F206_A1.3491.00
172_A176_L1.3481.00
265_W297_V1.3301.00
162_G203_G1.3051.00
145_A218_N1.2801.00
158_S206_A1.2681.00
121_D172_A1.2671.00
53_S60_G1.2621.00
225_P228_G1.2591.00
152_Q155_S1.2581.00
78_L95_Y1.2511.00
211_F217_L1.2390.99
143_R149_S1.2360.99
152_Q156_A1.2320.99
90_G142_Y1.2180.99
139_K156_A1.2120.99
140_F218_N1.2050.99
5_T154_K1.1870.99
134_I137_H1.1850.99
257_A268_G1.1710.99
152_Q210_V1.1650.99
73_G102_Y1.1630.99
165_Y197_F1.1540.99
17_R60_G1.1500.99
121_D174_S1.1480.99
81_Q96_Q1.1470.99
110_D113_G1.1440.99
206_A220_S1.1430.99
102_Y120_P1.1380.99
214_N262_S1.1350.99
186_P189_A1.1310.99
40_Y119_R1.1280.99
85_Y92_D1.1250.99
10_Y14_L1.1060.99
141_S152_Q1.1040.99
202_G224_G1.1030.99
217_L221_L1.0880.99
216_F296_Y1.0870.99
97_K125_R1.0810.99
91_I129_L1.0730.99
97_K123_K1.0650.98
4_T41_R1.0580.98
265_W299_Y1.0520.98
19_Y23_K1.0450.98
224_G228_G1.0450.98
110_D114_D1.0450.98
151_I263_K1.0390.98
6_Y154_K1.0350.98
115_P118_Q1.0330.98
2_Y7_I1.0180.98
136_N157_G1.0140.98
145_A206_A1.0140.98
214_N260_Y1.0100.98
179_D182_S1.0030.98
254_G273_Y1.0010.98
177_L180_E0.9960.98
130_N164_S0.9950.98
73_G83_N0.9900.97
105_D110_D0.9890.97
148_Q261_N0.9880.97
16_V299_Y0.9820.97
185_F189_A0.9810.97
148_Q206_A0.9800.97
92_D162_G0.9770.97
73_G116_Y0.9680.97
4_T153_K0.9650.97
106_F110_D0.9630.97
28_S37_S0.9610.97
17_R21_S0.9590.97
144_A156_A0.9580.97
260_Y267_A0.9570.97
236_Y239_L0.9560.97
10_Y300_R0.9540.97
15_T19_Y0.9510.97
237_E241_D0.9510.97
78_L81_Q0.9490.97
146_F257_A0.9480.97
141_S150_E0.9470.97
142_Y145_A0.9460.97
2_Y5_T0.9400.97
102_Y118_Q0.9390.97
136_N208_N0.9380.97
21_S62_S0.9350.96
229_Y246_T0.9320.96
83_N92_D0.9310.96
4_T8_E0.9280.96
202_G222_S0.9230.96
237_E240_E0.9170.96
178_P181_L0.9150.96
18_L48_L0.9140.96
43_N102_Y0.9120.96
20_L48_L0.9110.96
87_R272_V0.9070.96
175_S295_L0.9070.96
144_A152_Q0.9060.96
133_Y161_L0.9040.96
88_K134_I0.9020.96
119_R173_D0.8950.95
17_R148_Q0.8830.95
174_S177_L0.8810.95
100_G120_P0.8770.95
19_Y60_G0.8730.95
267_A297_V0.8720.95
185_F191_I0.8700.95
255_R272_V0.8670.95
85_Y272_V0.8610.94
144_A208_N0.8610.94
92_D272_V0.8600.94
92_D130_N0.8590.94
215_W267_A0.8590.94
172_A175_S0.8560.94
254_G271_F0.8540.94
108_D112_D0.8440.94
136_N205_Y0.8440.94
278_Y284_F0.8370.94
90_G93_L0.8340.93
198_D251_N0.8320.93
109_D112_D0.8300.93
161_L203_G0.8300.93
134_I158_S0.8290.93
225_P248_F0.8290.93
81_Q98_Y0.8260.93
272_V294_R0.8240.93
80_F95_Y0.8230.93
212_K215_W0.8210.93
20_L46_L0.8190.93
170_I216_F0.8130.92
169_R193_K0.8120.92
49_G204_G0.8090.92
262_S267_A0.8060.92
131_A161_L0.8030.92
102_Y116_Y0.7980.92
81_Q92_D0.7950.92
155_S205_Y0.7950.92
43_N100_G0.7940.92
159_F163_G0.7910.91
196_Y228_G0.7890.91
50_L61_Y0.7890.91
131_A163_G0.7890.91
7_I87_R0.7890.91
26_S41_R0.7890.91
255_R270_S0.7880.91
215_W260_Y0.7860.91
210_V213_K0.7860.91
119_R209_W0.7810.91
73_G81_Q0.7790.91
144_A148_Q0.7760.91
16_V50_L0.7730.90
122_L172_A0.7660.90
124_T274_N0.7630.90
273_Y289_S0.7610.90
42_P96_Q0.7580.90
41_R104_E0.7570.90
207_Y219_A0.7560.90
40_Y178_P0.7540.89
222_S225_P0.7520.89
48_L65_P0.7520.89
291_G294_R0.7510.89
288_N298_G0.7500.89
187_E190_N0.7480.89
17_R23_K0.7480.89
14_L52_F0.7480.89
139_K154_K0.7470.89
141_S148_Q0.7460.89
64_N68_L0.7460.89
125_R169_R0.7460.89
83_N255_R0.7450.89
221_L229_Y0.7440.89
99_K123_K0.7350.88
42_P148_Q0.7340.88
288_N291_G0.7330.88
54_Y57_L0.7250.87
160_L206_A0.7250.87
136_N152_Q0.7220.87
8_E151_I0.7220.87
226_G230_K0.7210.87
157_G204_G0.7210.87
155_S208_N0.7180.87
281_K284_F0.7170.87
14_L54_Y0.7130.87
252_L275_N0.7130.87
22_N25_N0.7110.86
112_D115_P0.7080.86
254_G258_L0.7060.86
215_W259_G0.7060.86
121_D176_L0.7040.86
119_R175_S0.7030.86
52_F269_L0.7010.86
96_Q200_G0.7000.86
202_G226_G0.6970.85
90_G130_N0.6960.85
31_S36_Q0.6950.85
162_G222_S0.6950.85
9_S12_D0.6920.85
225_P229_Y0.6910.85
155_S209_W0.6880.85
132_Y142_Y0.6860.84
88_K138_K0.6860.84
208_N259_G0.6850.84
9_S143_R0.6850.84
108_D114_D0.6830.84
52_F58_S0.6830.84
219_A254_G0.6820.84
153_K213_K0.6790.84
89_W256_L0.6780.84
136_N207_Y0.6780.84
227_L246_T0.6760.84
144_A150_E0.6730.83
274_N278_Y0.6690.83
10_Y13_K0.6670.83
96_Q255_R0.6660.83
80_F92_D0.6640.82
109_D113_G0.6640.82
92_D206_A0.6600.82
175_S179_D0.6590.82
64_N200_G0.6590.82
92_D146_F0.6580.82
2_Y8_E0.6570.82
167_Y195_K0.6570.82
85_Y90_G0.6570.82
86_G149_S0.6560.82
45_S64_N0.6560.82
141_S145_A0.6520.81
278_Y288_N0.6510.81
49_G162_G0.6500.81
79_D85_Y0.6490.81
2_Y15_T0.6470.81
141_S157_G0.6450.81
110_D115_P0.6450.81
169_R195_K0.6440.81
80_F83_N0.6420.80
292_N295_L0.6390.80
38_L177_L0.6390.80
23_K51_G0.6360.80
85_Y147_S0.6310.79
67_F71_N0.6300.79
95_Y127_F0.6290.79
269_L295_L0.6290.79
145_A152_Q0.6280.79
270_S294_R0.6240.78
81_Q128_G0.6220.78
129_L165_Y0.6210.78
179_D183_D0.6210.78
104_E217_L0.6190.78
43_N73_G0.6170.78
181_L185_F0.6160.78
158_S261_N0.6160.78
123_K171_K0.6140.77
52_F264_R0.6140.77
74_K195_K0.6140.77
49_G65_P0.6110.77
148_Q294_R0.6080.77
293_F297_V0.6080.77
76_K82_L0.6070.77
75_T102_Y0.6030.76
56_W62_S0.6030.76
163_G199_I0.6010.76
101_F124_T0.5990.76
19_Y29_L0.5970.75
43_N260_Y0.5960.75
284_F288_N0.5940.75
130_N253_D0.5920.75
8_E263_K0.5920.75
16_V104_E0.5910.75
139_K152_Q0.5900.74
209_W263_K0.5890.74
50_L59_L0.5850.74
99_K120_P0.5840.74
8_E11_P0.5840.74
75_T118_Q0.5840.74
182_S185_F0.5810.73
98_Y126_S0.5780.73
193_K273_Y0.5760.73
10_Y15_T0.5760.73
53_S151_I0.5760.73
64_N77_Y0.5760.73
227_L285_S0.5720.72
14_L55_R0.5710.72
15_T52_F0.5710.72
87_R143_R0.5700.72
281_K293_F0.5680.72
151_I262_S0.5670.72
132_Y253_D0.5670.72
83_N90_G0.5670.72
89_W201_L0.5630.71
84_L91_I0.5620.71
85_Y148_Q0.5610.71
132_Y145_A0.5610.71
275_N283_D0.5600.71
108_D111_D0.5590.70
148_Q216_F0.5590.70
239_L242_S0.5580.70
144_A257_A0.5580.70
220_S224_G0.5570.70
126_S132_Y0.5570.70
184_Y188_E0.5570.70
224_G227_L0.5560.70
31_S34_N0.5560.70
19_Y27_F0.5540.70
15_T21_S0.5540.70
57_L164_S0.5530.70
21_S24_Y0.5530.70
32_K40_Y0.5530.70
92_D253_D0.5520.70
7_I150_E0.5520.70
237_E242_S0.5510.69
35_G112_D0.5500.69
73_G76_K0.5500.69
100_G103_L0.5490.69
209_W236_Y0.5480.69
106_F111_D0.5460.69
146_F222_S0.5430.68
281_K287_N0.5420.68
152_Q158_S0.5390.68
209_W256_L0.5390.68
140_F222_S0.5380.68
13_K20_L0.5380.68
14_L20_L0.5380.68
170_I183_D0.5370.67
227_L239_L0.5360.67
132_Y160_L0.5340.67
134_I234_G0.5310.67
198_D268_G0.5310.67
253_D288_N0.5300.66
18_L34_N0.5280.66
271_F293_F0.5280.66
225_P286_I0.5270.66
209_W212_K0.5260.66
56_W143_R0.5260.66
35_G241_D0.5260.66
7_I10_Y0.5250.66
161_L201_L0.5240.66
270_S296_Y0.5230.65
150_E247_N0.5220.65
183_D235_T0.5210.65
114_D127_F0.5210.65
136_N235_T0.5210.65
126_S224_G0.5210.65
128_G164_S0.5200.65
74_K245_K0.5200.65
276_Y280_N0.5200.65
90_G134_I0.5170.65
256_L269_L0.5170.65
53_S270_S0.5160.64
142_Y257_A0.5160.64
148_Q268_G0.5150.64
58_S149_S0.5150.64
282_S289_S0.5140.64
28_S180_E0.5130.64
273_Y276_Y0.5120.64
16_V34_N0.5110.64
42_P226_G0.5110.64
36_Q210_V0.5110.64
85_Y146_F0.5100.64
141_S144_A0.5090.63
54_Y251_N0.5080.63
79_D128_G0.5070.63
47_N62_S0.5050.63
147_S196_Y0.5050.63
119_R176_L0.5050.63
147_S208_N0.5040.63
37_S265_W0.5040.63
28_S39_R0.5040.63
15_T266_F0.5040.63
75_T120_P0.5010.62
103_L178_P0.5010.62
220_S223_L0.5000.62
140_F208_N0.5000.62
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1p4tA 2 0.515 51.6 0.936 Contact Map
1xkwA 1 0.8173 39.5 0.941 Contact Map
3efmA 1 0.6844 35.4 0.942 Contact Map
2f1vA 4 0.5249 30.7 0.944 Contact Map
2x27X 1 0.5914 28.8 0.945 Contact Map
3nb3A 2 0.4385 24.9 0.947 Contact Map
2lhfA 1 0.5648 22.8 0.948 Contact Map
3qlbA 1 0.7076 20.5 0.949 Contact Map
1ormA 1 0.4751 20.3 0.949 Contact Map
2ervA 1 0.4718 16.4 0.951 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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