GREMLIN Database
DUF4420 - Putative PD-(D/E)XK family member, (DUF4420)
PFAM: PF14390 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 306 (300)
Sequences: 2920 (2559)
Seq/√Len: 147.8
META: 0.791

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
149_R233_E6.3781.00
143_G186_T6.2281.00
235_R249_E4.9901.00
147_F182_I3.9401.00
59_P63_G3.6371.00
169_E251_K3.6251.00
298_D302_S3.1811.00
149_R237_L3.0501.00
96_D117_R2.9101.00
247_L251_K2.8851.00
232_D262_E2.6501.00
238_L248_F2.5761.00
148_L161_A2.5601.00
164_A177_F2.5151.00
229_D269_R2.4361.00
195_V215_S2.4351.00
234_I238_L2.4251.00
225_E233_E2.4051.00
147_F184_V2.3741.00
153_D237_L2.3691.00
145_L234_I2.3221.00
61_L64_I2.2161.00
36_D40_N2.2131.00
196_H300_R2.2031.00
123_E126_R2.1951.00
135_E270_F2.1831.00
226_S269_R2.1291.00
200_L299_V2.0911.00
166_T171_A2.0361.00
142_F230_L1.9971.00
200_L287_I1.9861.00
133_L265_Y1.9841.00
201_E289_R1.9801.00
86_L124_L1.9311.00
31_L98_I1.9111.00
154_P157_G1.9091.00
295_G303_I1.9081.00
228_P257_Y1.9081.00
22_R30_D1.9011.00
139_R186_T1.8971.00
196_H302_S1.8251.00
214_A277_L1.8141.00
235_R259_D1.8011.00
278_Y284_F1.7871.00
173_Q181_A1.7871.00
231_V257_Y1.7571.00
293_P296_V1.7461.00
96_D121_W1.7411.00
257_Y265_Y1.7221.00
186_T218_L1.7071.00
200_L292_L1.6981.00
142_F225_E1.6801.00
147_F216_V1.6701.00
146_L216_V1.6461.00
20_A34_G1.6421.00
205_P286_R1.6071.00
34_G42_R1.6071.00
297_S304_D1.5951.00
149_R225_E1.5731.00
235_R245_L1.5411.00
159_E241_D1.5181.00
173_Q211_L1.4991.00
228_P266_D1.4971.00
133_L137_E1.4821.00
22_R32_F1.4731.00
29_L110_A1.4701.00
227_L265_Y1.4451.00
181_A209_G1.4421.00
153_D157_G1.4421.00
292_L295_G1.4311.00
163_E247_L1.4211.00
139_R143_G1.4001.00
61_L67_S1.3991.00
280_V284_F1.3961.00
134_S137_E1.3951.00
33_A94_C1.3951.00
99_E102_E1.3811.00
31_L101_L1.3801.00
139_R218_L1.3791.00
146_L230_L1.3781.00
64_I67_S1.3751.00
184_V214_A1.3661.00
173_Q176_E1.3651.00
149_R234_I1.3611.00
138_Q268_R1.3601.00
4_P33_A1.3531.00
142_F146_L1.3521.00
171_A176_E1.3471.00
150_R275_E1.3471.00
195_V213_L1.3421.00
187_T215_S1.3371.00
146_L149_R1.3281.00
288_T291_D1.2891.00
292_L299_V1.2831.00
146_L272_L1.2831.00
245_L249_E1.2801.00
59_P64_I1.2521.00
63_G66_L1.2431.00
119_E122_Q1.2411.00
83_D86_L1.2341.00
135_E139_R1.2191.00
172_R201_E1.2181.00
214_A275_E1.2181.00
241_D244_A1.2081.00
162_V244_A1.2001.00
74_G77_L1.1931.00
259_D262_E1.1901.00
166_T176_E1.1891.00
41_R87_R1.1881.00
147_F214_A1.1881.00
100_A117_R1.1801.00
159_E244_A1.1731.00
63_G67_S1.1661.00
149_R230_L1.1641.00
231_V252_L1.1601.00
224_G229_D1.1481.00
151_L154_P1.1370.99
234_I248_F1.1360.99
60_S63_G1.1350.99
95_E99_E1.1300.99
142_F218_L1.1290.99
176_E181_A1.1260.99
36_D39_G1.1200.99
257_Y262_E1.0990.99
117_R120_R1.0930.99
138_Q265_Y1.0880.99
229_D232_D1.0850.99
252_L257_Y1.0790.99
206_P209_G1.0720.99
204_D289_R1.0690.99
27_H101_L1.0640.99
138_Q227_L1.0630.99
204_D290_S1.0610.99
166_T251_K1.0520.99
194_R302_S1.0520.99
75_R78_V1.0280.99
141_L175_F1.0150.99
73_G76_Y1.0080.99
115_L119_E1.0040.99
157_G160_E0.9990.99
216_V275_E0.9990.99
287_I291_D0.9970.99
133_L227_L0.9950.99
4_P21_R0.9760.98
91_A95_E0.9720.98
187_T217_R0.9650.98
142_F272_L0.9630.98
194_R297_S0.9610.98
36_D80_T0.9530.98
158_P238_L0.9450.98
148_L175_F0.9370.98
42_R78_V0.9350.98
106_D109_E0.9300.98
146_L225_E0.9290.98
203_L211_L0.9270.98
6_D10_A0.9220.98
193_R215_S0.9150.98
20_A32_F0.9120.98
152_L161_A0.9100.98
36_D42_R0.9070.98
143_G216_V0.9020.97
93_L125_F0.9020.97
35_V39_G0.9000.97
71_E77_L0.9000.97
261_H265_Y0.8950.97
287_I293_P0.8910.97
180_G212_F0.8860.97
137_E255_A0.8850.97
141_L257_Y0.8800.97
169_E254_A0.8730.97
33_A42_R0.8680.97
91_A94_C0.8670.97
250_E254_A0.8660.97
153_D156_L0.8650.97
184_V216_V0.8630.97
63_G76_Y0.8540.96
187_T190_S0.8540.96
172_R199_S0.8530.96
73_G77_L0.8510.96
286_R295_G0.8470.96
166_T169_E0.8450.96
97_L118_L0.8440.96
198_S300_R0.8430.96
59_P62_K0.8390.96
135_E268_R0.8350.96
139_R187_T0.8330.96
61_L66_L0.8320.96
220_E224_G0.8280.96
200_L301_Y0.8260.96
284_F303_I0.8260.96
204_D288_T0.8250.96
34_G43_L0.8240.96
167_G255_A0.8240.96
90_F121_W0.8200.95
4_P7_E0.8190.95
11_P19_R0.8160.95
24_D27_H0.8120.95
146_L150_R0.8090.95
181_A211_L0.8080.95
287_I299_V0.8020.95
248_F251_K0.7980.95
133_L257_Y0.7960.95
236_E239_S0.7940.95
140_G175_F0.7930.95
43_L46_V0.7910.95
92_A96_D0.7910.95
187_T192_G0.7910.95
225_E230_L0.7860.94
230_L233_E0.7850.94
81_L87_R0.7800.94
90_F94_C0.7760.94
139_R270_F0.7760.94
19_R22_R0.7740.94
147_F151_L0.7730.94
162_V238_L0.7700.94
297_S302_S0.7690.94
42_R47_S0.7690.94
139_R200_L0.7630.93
251_K255_A0.7610.93
213_L218_L0.7610.93
117_R125_F0.7560.93
200_L297_S0.7550.93
195_V305_L0.7550.93
18_L22_R0.7550.93
160_E163_E0.7540.93
228_P231_V0.7530.93
221_S225_E0.7510.93
60_S64_I0.7470.93
61_L65_E0.7470.93
227_L231_V0.7460.93
132_G265_Y0.7410.92
136_E139_R0.7410.92
252_L265_Y0.7400.92
281_D286_R0.7390.92
24_D102_E0.7370.92
153_D158_P0.7350.92
150_R154_P0.7320.92
261_H266_D0.7290.92
17_G20_A0.7280.92
232_D236_E0.7270.92
177_F180_G0.7270.92
71_E75_R0.7250.92
152_L156_L0.7250.92
88_D117_R0.7250.92
282_D286_R0.7230.91
63_G69_R0.7230.91
211_L280_V0.7230.91
20_A24_D0.7230.91
192_G304_D0.7230.91
152_L157_G0.7200.91
232_D235_R0.7170.91
68_R77_L0.7170.91
43_L48_F0.7130.91
100_A120_R0.7090.91
217_R274_E0.7090.91
168_P255_A0.7080.91
141_L227_L0.7070.91
143_G187_T0.7070.91
210_P279_E0.7070.91
255_A261_H0.7070.91
142_F186_T0.7070.91
64_I68_R0.7040.90
59_P66_L0.7030.90
197_I213_L0.7020.90
20_A25_P0.7010.90
167_G170_G0.6990.90
242_P245_L0.6970.90
138_Q228_P0.6960.90
138_Q261_H0.6920.90
71_E76_Y0.6910.90
133_L256_G0.6890.89
7_E12_S0.6870.89
198_S213_L0.6860.89
121_W256_G0.6850.89
172_R206_P0.6850.89
233_E236_E0.6830.89
64_I70_S0.6830.89
79_L118_L0.6820.89
182_I212_F0.6780.89
9_E12_S0.6730.88
162_V247_L0.6700.88
142_F270_F0.6640.88
238_L243_E0.6620.87
21_R40_N0.6610.87
145_L231_V0.6610.87
132_G264_L0.6600.87
7_E10_A0.6590.87
41_R91_A0.6580.87
223_N271_T0.6550.87
201_E298_D0.6530.87
213_L303_I0.6530.87
123_E246_E0.6490.86
36_D41_R0.6490.86
64_I78_V0.6480.86
226_S266_D0.6480.86
24_D28_P0.6470.86
170_G255_A0.6460.86
67_S73_G0.6460.86
31_L43_L0.6440.86
89_I120_R0.6440.86
262_E265_Y0.6430.86
20_A27_H0.6420.86
79_L180_G0.6410.86
7_E22_R0.6360.85
16_G24_D0.6360.85
228_P265_Y0.6350.85
180_G210_P0.6350.85
4_P8_L0.6340.85
65_E68_R0.6330.85
268_R305_L0.6290.85
36_D43_L0.6280.85
168_P251_K0.6270.84
122_Q126_R0.6270.84
117_R121_W0.6270.84
227_L257_Y0.6250.84
89_I138_Q0.6230.84
186_T216_V0.6220.84
101_L113_A0.6220.84
42_R48_F0.6190.84
238_L244_A0.6170.84
122_Q127_K0.6170.84
63_G68_R0.6170.84
17_G26_D0.6160.83
228_P259_D0.6160.83
156_L161_A0.6140.83
138_Q270_F0.6140.83
116_N283_D0.6130.83
6_D11_P0.6120.83
189_S192_G0.6120.83
167_G197_I0.6120.83
38_P52_S0.6120.83
41_R44_L0.6110.83
23_V27_H0.6110.83
5_W173_Q0.6090.83
94_C124_L0.6090.83
183_E301_Y0.6050.82
215_S278_Y0.6050.82
86_L89_I0.6040.82
117_R253_A0.6040.82
11_P35_V0.6020.82
124_L128_G0.6010.82
197_I284_F0.6000.82
148_L151_L0.5980.82
125_F195_V0.5960.81
19_R24_D0.5910.81
157_G161_A0.5900.81
6_D9_E0.5880.81
97_L252_L0.5870.80
9_E87_R0.5870.80
39_G43_L0.5870.80
182_I214_A0.5860.80
133_L252_L0.5860.80
42_R46_V0.5850.80
138_Q142_F0.5840.80
123_E249_E0.5830.80
107_E282_D0.5830.80
81_L93_L0.5830.80
87_R91_A0.5820.80
283_D291_D0.5820.80
7_E13_S0.5810.80
43_L94_C0.5800.80
79_L93_L0.5790.80
30_D45_L0.5760.79
154_P207_G0.5760.79
172_R191_S0.5740.79
93_L124_L0.5730.79
133_L138_Q0.5720.79
192_G201_E0.5720.79
63_G77_L0.5720.79
147_F277_L0.5700.78
60_S67_S0.5700.78
146_L218_L0.5700.78
186_T235_R0.5700.78
203_L284_F0.5700.78
270_F273_R0.5680.78
172_R181_A0.5670.78
71_E74_G0.5670.78
194_R298_D0.5660.78
4_P125_F0.5640.78
60_S66_L0.5630.78
189_S201_E0.5620.77
22_R26_D0.5620.77
163_E271_T0.5620.77
17_G38_P0.5610.77
74_G78_V0.5600.77
35_V41_R0.5560.77
195_V303_I0.5550.77
245_L248_F0.5550.77
233_E237_L0.5550.77
89_I117_R0.5550.77
139_R215_S0.5530.76
6_D12_S0.5530.76
153_D161_A0.5520.76
212_F277_L0.5520.76
26_D29_L0.5520.76
295_G304_D0.5510.76
48_F107_E0.5510.76
150_R153_D0.5500.76
213_L278_Y0.5480.76
38_P48_F0.5470.76
81_L125_F0.5470.76
47_S111_L0.5460.75
239_S242_P0.5460.75
197_I203_L0.5450.75
124_L191_S0.5430.75
78_V81_L0.5430.75
7_E21_R0.5410.75
166_T252_L0.5370.74
62_K153_D0.5370.74
163_E300_R0.5360.74
159_E247_L0.5350.74
268_R273_R0.5350.74
121_W265_Y0.5300.73
252_L266_D0.5290.73
193_R217_R0.5280.73
44_L91_A0.5270.73
96_D204_D0.5260.73
70_S73_G0.5250.73
281_D303_I0.5230.72
4_P10_A0.5230.72
195_V302_S0.5210.72
151_L277_L0.5210.72
240_S252_L0.5200.72
23_V188_S0.5200.72
16_G19_R0.5190.72
153_D234_I0.5190.72
19_R25_P0.5190.72
215_S218_L0.5170.71
157_G162_V0.5150.71
161_A175_F0.5150.71
220_E293_P0.5140.71
19_R27_H0.5120.71
5_W8_L0.5120.71
33_A43_L0.5110.71
12_S16_G0.5110.71
10_A16_G0.5110.71
123_E250_E0.5090.70
67_S76_Y0.5090.70
158_P163_E0.5080.70
81_L195_V0.5070.70
10_A35_V0.5070.70
293_P297_S0.5060.70
217_R273_R0.5050.70
12_S28_P0.5050.70
20_A23_V0.5030.69
218_L272_L0.5030.69
43_L47_S0.5020.69
50_D73_G0.5010.69
143_G181_A0.5010.69
44_L182_I0.5000.69
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1ob8A 1 0.3235 74.6 0.931 Contact Map
1hh1A 2 0.3137 69.9 0.933 Contact Map
2wcwA 2 0.3072 58.9 0.938 Contact Map
2eo0A 2 0.3137 47.2 0.942 Contact Map
1gefA 2 0.3137 30.1 0.948 Contact Map
3fovA 2 0.3105 21.2 0.952 Contact Map
1y0kA 1 0.4902 15.7 0.955 Contact Map
2oa5A 4 0.2614 14.2 0.955 Contact Map
2ostA 4 0.3693 7.5 0.961 Contact Map
2g30A 1 0.3072 5.7 0.963 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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