GREMLIN Database
DUF4400 - Domain of unknown function (DUF4400)
PFAM: PF14348 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 204 (195)
Sequences: 1148 (801)
Seq/√Len: 57.4
META: 0.636

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
140_D143_R4.5691.00
8_E11_Q4.0531.00
133_V137_V3.3821.00
132_L200_A3.2161.00
48_Q53_K2.5551.00
127_A131_A2.5151.00
155_H158_K2.4181.00
123_L185_L2.2771.00
133_V136_L2.2501.00
99_Y102_A2.2021.00
131_A193_G2.1831.00
140_D144_F2.0991.00
2_W5_Q2.0571.00
120_W151_P2.0071.00
198_I201_A1.8831.00
12_A15_E1.8451.00
134_D197_A1.8200.99
121_L125_L1.8020.99
8_E18_L1.7960.99
5_Q12_A1.7140.99
141_L145_G1.7130.99
195_A198_I1.6950.99
47_Y59_W1.6780.99
65_A68_A1.6590.99
185_L188_F1.6480.99
186_L199_T1.6350.99
40_L44_D1.6290.99
84_G87_N1.6260.99
178_S184_I1.6170.99
48_Q52_V1.5910.99
71_G74_G1.5810.98
4_E8_E1.5800.98
186_L195_A1.5450.98
28_S34_P1.5420.98
147_G154_Y1.5350.98
139_R144_F1.4960.98
138_R197_A1.4720.97
10_S105_Y1.4560.97
52_V55_G1.4560.97
122_P150_S1.4470.97
152_F203_F1.4200.97
43_A108_L1.4100.97
152_F156_R1.3910.96
41_R45_A1.3850.96
142_R150_S1.3790.96
142_R149_E1.3690.96
116_I203_F1.3690.96
145_G179_L1.3650.96
69_A72_Q1.3650.96
130_V143_R1.3600.96
104_M143_R1.3570.96
160_L195_A1.3510.96
10_S13_M1.3380.96
71_G94_V1.2970.95
118_L121_L1.2800.94
160_L164_L1.2730.94
142_R145_G1.2690.94
134_D139_R1.2670.94
97_E101_L1.2570.94
189_A200_A1.2560.94
197_A201_A1.2550.94
93_Y97_E1.2540.94
58_D114_L1.2530.94
99_Y154_Y1.2520.94
67_A70_S1.2460.94
74_G179_L1.2260.93
64_S93_Y1.2260.93
42_R46_L1.2000.92
126_L130_V1.1980.92
19_G23_E1.1950.92
25_F28_S1.1760.91
99_Y139_R1.1760.91
180_H183_L1.1730.91
108_L142_R1.1710.91
149_E155_H1.1690.91
51_F104_M1.1630.91
154_Y158_K1.1580.91
34_P163_P1.1550.91
144_F156_R1.1480.90
140_D149_E1.1420.90
194_L198_I1.1380.90
25_F179_L1.1270.90
107_T129_L1.1260.89
191_L195_A1.1110.89
21_L112_V1.0970.88
134_D137_V1.0960.88
100_L104_M1.0930.88
8_E13_M1.0840.88
132_L203_F1.0810.87
139_R143_R1.0810.87
11_Q15_E1.0790.87
142_R146_A1.0760.87
136_L149_E1.0680.87
185_L195_A1.0640.87
197_A200_A1.0640.87
111_L115_A1.0580.86
142_R151_P1.0550.86
123_L144_F1.0550.86
116_I155_H1.0520.86
19_G32_G1.0480.86
8_E22_S1.0360.85
177_V197_A1.0330.85
87_N91_Q1.0330.85
100_L141_L1.0310.85
9_H13_M1.0250.85
148_R151_P1.0190.84
140_D163_P1.0150.84
62_R67_A1.0140.84
47_Y54_T1.0130.84
124_F173_L1.0130.84
54_T66_P0.9950.83
172_Y181_P0.9860.82
16_A125_L0.9790.82
146_A149_E0.9790.82
110_F187_P0.9700.81
144_F151_P0.9700.81
68_A71_G0.9690.81
41_R118_L0.9680.81
132_L194_L0.9640.81
186_L191_L0.9620.81
119_L154_Y0.9570.80
13_M184_I0.9530.80
32_G38_T0.9470.80
122_P181_P0.9400.79
172_Y185_L0.9370.79
194_L200_A0.9280.78
199_T203_F0.9270.78
135_G149_E0.9240.78
136_L139_R0.9150.77
175_L178_S0.9130.77
131_A147_G0.9130.77
37_R165_L0.9060.76
33_E50_L0.9000.76
197_A202_S0.8940.75
189_A193_G0.8920.75
137_V141_L0.8920.75
186_L189_A0.8860.75
103_A142_R0.8840.75
46_L111_L0.8800.74
137_V181_P0.8690.73
57_L197_A0.8670.73
135_G147_G0.8630.73
68_A73_G0.8590.72
84_G88_W0.8590.72
18_L23_E0.8570.72
29_L159_R0.8550.72
57_L61_Q0.8520.72
21_L97_E0.8500.72
104_M114_L0.8460.71
33_E37_R0.8450.71
136_L187_P0.8430.71
144_F147_G0.8410.71
32_G35_A0.8250.69
153_V169_W0.8230.69
139_R162_I0.8190.69
23_E26_T0.8170.69
58_D62_R0.8140.68
16_A19_G0.8060.68
39_A133_V0.8050.68
19_G92_L0.8030.67
47_Y57_L0.8010.67
102_A183_L0.7990.67
69_A125_L0.7830.65
162_I179_L0.7830.65
47_Y104_M0.7780.65
156_R159_R0.7780.65
133_V139_R0.7750.65
92_L96_L0.7740.64
41_R54_T0.7660.64
39_A119_L0.7650.64
130_V168_P0.7640.63
49_W190_V0.7630.63
123_L127_A0.7620.63
128_A196_V0.7590.63
108_L158_K0.7590.63
129_L132_L0.7490.62
52_V57_L0.7430.61
3_P7_W0.7420.61
156_R165_L0.7410.61
112_V115_A0.7360.61
69_A73_G0.7330.60
122_P131_A0.7330.60
3_P16_A0.7320.60
198_I202_S0.7320.60
173_L203_F0.7310.60
185_L189_A0.7310.60
83_R87_N0.7250.59
87_N90_G0.7250.59
83_R86_R0.7250.59
14_L112_V0.7240.59
140_D172_Y0.7240.59
155_H173_L0.7150.58
60_L104_M0.7120.58
41_R172_Y0.7110.58
139_R154_Y0.7110.58
130_V142_R0.7110.58
45_A49_W0.7110.58
23_E65_A0.7090.58
164_L189_A0.7080.58
172_Y187_P0.7070.57
93_Y96_L0.7050.57
168_P180_H0.7020.57
61_Q65_A0.6990.57
150_S168_P0.6940.56
131_A197_A0.6930.56
135_G172_Y0.6920.56
49_W53_K0.6900.56
2_W9_H0.6900.56
27_R32_G0.6840.55
51_F54_T0.6830.55
66_P69_A0.6750.54
147_G155_H0.6740.54
112_V142_R0.6700.53
3_P90_G0.6690.53
101_L109_L0.6640.53
166_V175_L0.6500.51
24_H99_Y0.6470.51
62_R68_A0.6440.51
120_W149_E0.6440.51
21_L26_T0.6440.51
51_F55_G0.6440.51
63_A69_A0.6400.50
75_A125_L0.6400.50
20_Y159_R0.6390.50
102_A156_R0.6380.50
70_S73_G0.6360.50
102_A143_R0.6360.50
198_I204_K0.6320.49
150_S159_R0.6300.49
117_L169_W0.6300.49
13_M109_L0.6270.49
128_A192_L0.6240.48
3_P12_A0.6240.48
37_R137_V0.6230.48
63_A94_V0.6220.48
51_F57_L0.6220.48
4_E7_W0.6180.48
149_E172_Y0.6180.48
126_L180_H0.6100.47
109_L123_L0.6080.47
188_F193_G0.6080.47
68_A72_Q0.6050.46
7_W12_A0.6050.46
57_L65_A0.6020.46
141_L184_I0.6020.46
102_A122_P0.5960.45
47_Y51_F0.5910.45
156_R173_L0.5890.45
136_L140_D0.5880.44
148_R180_H0.5870.44
6_G10_S0.5840.44
39_A71_G0.5820.44
48_Q58_D0.5810.44
16_A175_L0.5800.44
39_A137_V0.5790.44
145_G159_R0.5780.43
157_A163_P0.5740.43
53_K127_A0.5730.43
55_G62_R0.5700.43
101_L125_L0.5690.42
123_L188_F0.5640.42
115_A178_S0.5640.42
59_W62_R0.5630.42
122_P126_L0.5620.42
187_P198_I0.5600.41
155_H168_P0.5500.40
88_W129_L0.5500.40
126_L154_Y0.5430.40
46_L62_R0.5400.39
30_L164_L0.5390.39
27_R126_L0.5380.39
53_K125_L0.5360.39
57_L139_R0.5330.39
85_F89_L0.5320.39
51_F66_P0.5320.39
131_A140_D0.5300.38
117_L122_P0.5290.38
16_A20_Y0.5240.38
38_T111_L0.5220.37
28_S138_R0.5190.37
164_L168_P0.5190.37
3_P8_E0.5150.37
20_Y164_L0.5090.36
45_A48_Q0.5050.36
66_P90_G0.5030.36
108_L120_W0.5020.35
66_P93_Y0.5010.35
104_M139_R0.5010.35
46_L50_L0.5010.35
59_W129_L0.5000.35
92_L95_W0.5000.35
7_W11_Q0.5000.35
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
5ajiA 3 0.4902 39.3 0.922 Contact Map
4hw9A 3 0.4412 24.2 0.93 Contact Map
3udcA 3 0.4706 9.5 0.942 Contact Map
4p02A 1 0.25 3.4 0.953 Contact Map
4a2nB 1 0.4412 2.8 0.955 Contact Map
2xq2A 2 0.5637 2.2 0.957 Contact Map
2onkC 2 0.1912 2 0.959 Contact Map
4m7cA 2 0.5245 1.9 0.959 Contact Map
4r7jA 3 0.3088 1.4 0.962 Contact Map
2q32A 1 0.2745 1.3 0.963 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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