GREMLIN Database
DUF4396 - Domain of unknown function (DUF4396)
PFAM: PF14342 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 141 (131)
Sequences: 6310 (3004)
Seq/√Len: 262.4
META: 0.894

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
27_G41_T3.8581.00
122_A126_V2.9961.00
6_A129_Y2.6841.00
68_S71_A2.6661.00
51_L55_A2.4261.00
59_L63_R2.4061.00
50_F81_T2.3421.00
62_L69_L2.3231.00
86_V131_V2.3141.00
46_I85_A2.2701.00
26_I34_L2.1731.00
82_L131_V2.0451.00
94_V98_V2.0071.00
36_W40_A1.8991.00
94_V119_I1.8951.00
70_R74_R1.8211.00
23_G48_L1.7511.00
115_G119_I1.7391.00
50_F77_L1.7241.00
133_R137_A1.7131.00
58_T72_A1.6281.00
22_L52_F1.6091.00
91_D120_A1.5721.00
24_M45_A1.5081.00
71_A74_R1.5051.00
6_A125_F1.5001.00
18_I56_L1.4871.00
42_I96_L1.4861.00
111_P115_G1.4861.00
85_A89_I1.4751.00
6_A10_L1.4611.00
26_I48_L1.4171.00
54_Y81_T1.4051.00
10_L14_T1.3781.00
90_V123_V1.3441.00
93_A96_L1.2951.00
94_V123_V1.2791.00
73_L77_L1.2661.00
107_G110_D1.2611.00
25_V28_T1.2331.00
99_P102_M1.2321.00
119_I123_V1.2281.00
47_V51_L1.2231.00
100_G103_D1.2061.00
90_V127_T1.1981.00
8_A60_P1.1901.00
86_V127_T1.1721.00
61_L67_L1.1491.00
7_S10_L1.1371.00
125_F129_Y1.1311.00
39_L43_A1.1281.00
126_V129_Y1.1151.00
58_T73_L1.1101.00
56_L59_L1.1011.00
43_A47_V1.1001.00
49_A89_I1.0971.00
71_A75_T1.0921.00
129_Y133_R1.0911.00
55_A59_L1.0891.00
37_S40_A1.0711.00
57_T76_A1.0641.00
19_G23_G1.0631.00
99_P103_D1.0571.00
33_A108_L1.0461.00
67_L75_T1.0331.00
27_G44_L1.0331.00
68_S74_R1.0211.00
26_I44_L1.0161.00
39_L42_I1.0061.00
126_V130_P1.0021.00
127_T130_P0.9981.00
17_A121_L0.9881.00
98_V116_S0.9821.00
50_F54_Y0.9801.00
58_T69_L0.9731.00
64_A67_L0.9721.00
14_T17_A0.9671.00
134_W137_A0.9651.00
62_L66_G0.9551.00
112_L115_G0.9471.00
58_T62_L0.9351.00
40_A43_A0.9251.00
67_L71_A0.9211.00
88_E92_N0.9181.00
36_W39_L0.9181.00
94_V115_G0.9161.00
69_L73_L0.9101.00
23_G44_L0.8991.00
38_N42_I0.8961.00
61_L75_T0.8941.00
22_L25_V0.8701.00
94_V116_S0.8701.00
16_C20_E0.8671.00
62_L68_S0.8621.00
10_L125_F0.8601.00
14_T18_I0.8521.00
77_L81_T0.8460.99
45_A92_N0.8440.99
90_V94_V0.8380.99
5_A129_Y0.8360.99
93_A97_L0.8330.99
62_L72_A0.8090.99
5_A133_R0.8080.99
97_L101_A0.8050.99
48_L52_F0.7990.99
134_W138_R0.7820.99
72_A75_T0.7670.99
82_L86_V0.7660.99
17_A21_V0.7650.99
26_I33_A0.7620.99
95_M120_A0.7610.99
101_A116_S0.7590.99
112_L116_S0.7540.99
115_G118_A0.7530.99
74_R77_L0.7520.99
28_T108_L0.7440.99
59_L74_R0.7440.99
42_I92_N0.7430.99
100_G104_A0.7400.99
9_T125_F0.7350.99
122_A125_F0.7300.98
121_L130_P0.7280.98
54_Y73_L0.7270.98
12_C16_C0.7230.98
125_F128_A0.7080.98
20_E24_M0.7070.98
67_L72_A0.7000.98
95_M117_L0.6990.98
42_I46_I0.6890.98
23_G27_G0.6840.98
4_Q8_A0.6840.98
55_A60_P0.6770.98
21_V117_L0.6750.97
58_T74_R0.6740.97
45_A120_A0.6730.97
75_T79_A0.6730.97
57_T79_A0.6660.97
98_V113_F0.6640.97
24_M102_M0.6640.97
74_R82_L0.6620.97
53_G80_D0.6560.97
96_L102_M0.6560.97
102_M116_S0.6340.96
12_C15_G0.6330.96
28_T109_G0.6260.96
19_G88_E0.6250.96
37_S41_T0.6250.96
89_I93_A0.6230.96
24_M95_M0.6210.96
15_G19_G0.6210.96
95_M116_S0.6130.96
57_T80_D0.6120.96
26_I40_A0.6120.96
36_W41_T0.6120.96
15_G83_S0.6110.96
25_V108_L0.6080.95
116_S119_I0.6070.95
16_C88_E0.6070.95
18_I22_L0.6050.95
7_S14_T0.6040.95
46_I50_F0.5970.95
8_A136_I0.5960.95
4_Q7_S0.5950.95
90_V119_I0.5950.95
58_T61_L0.5920.95
11_H53_G0.5910.95
38_N103_D0.5900.95
58_T76_A0.5890.95
24_M41_T0.5880.95
62_L71_A0.5870.94
60_P63_R0.5840.94
24_M28_T0.5820.94
74_R78_A0.5810.94
70_R77_L0.5810.94
125_F134_W0.5740.94
3_R7_S0.5720.94
9_T128_A0.5700.94
98_V114_W0.5690.94
79_A135_L0.5680.93
26_I36_W0.5630.93
7_S59_L0.5550.93
25_V109_G0.5550.93
50_F84_I0.5500.92
68_S72_A0.5480.92
90_V95_M0.5450.92
78_A140_K0.5410.92
40_A44_L0.5330.91
59_L62_L0.5320.91
103_D111_P0.5310.91
61_L72_A0.5290.91
35_G40_A0.5250.90
44_L48_L0.5240.90
134_W140_K0.5080.89
19_G50_F0.5060.89
42_I45_A0.5050.89
34_L44_L0.5000.88
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3aqpA 1 0.9078 59 0.901 Contact Map
3ne5A 3 0.922 48.5 0.907 Contact Map
3w9iA 3 0.922 43.2 0.91 Contact Map
4dx5A 3 0.8936 42.7 0.91 Contact Map
4k0jA 3 0.8794 38.8 0.912 Contact Map
4mt1A 3 0.9504 27.9 0.919 Contact Map
4httA 1 0.5177 21.8 0.923 Contact Map
4b4aA 1 0.6028 9.8 0.934 Contact Map
1zcdA 1 0.4255 8.5 0.936 Contact Map
2gfpA 2 0.9716 7.1 0.938 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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