GREMLIN Database
DUF4391 - Domain of unknown function (DUF4391)
PFAM: PF14335 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 242 (212)
Sequences: 2364 (1706)
Seq/√Len: 117.2
META: 0.824

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
7_T43_Y3.3111.00
30_K34_D2.8901.00
217_A221_K2.8271.00
227_R230_E2.7941.00
75_K102_K2.6501.00
69_E98_E2.5681.00
216_E239_K2.5631.00
212_I235_L2.5281.00
234_E237_K2.4891.00
5_K8_E2.4771.00
59_G165_D2.4691.00
42_L69_E2.4071.00
204_E241_E2.3811.00
37_E73_K2.2851.00
219_L231_L2.2691.00
204_E207_K2.2271.00
222_E227_R2.2081.00
214_K218_K2.2021.00
16_K20_E2.1401.00
225_F229_V2.1151.00
230_E234_E2.0901.00
19_Y33_V2.0471.00
107_A175_I1.9961.00
19_Y32_F1.9951.00
35_Q78_D1.9351.00
229_V232_N1.9171.00
41_W66_Q1.9171.00
212_I238_L1.8841.00
225_F232_N1.8821.00
211_E234_E1.8771.00
86_D106_S1.8241.00
160_P170_N1.7871.00
58_A63_P1.7861.00
236_K239_K1.7511.00
193_E201_R1.7441.00
66_Q91_Y1.7391.00
12_R38_R1.7161.00
116_D119_K1.7121.00
219_L235_L1.6931.00
13_I68_F1.6861.00
163_N166_A1.6781.00
237_K241_E1.6391.00
104_Q127_A1.5791.00
113_S116_D1.5741.00
26_A30_K1.5661.00
86_D110_K1.5531.00
209_E213_A1.5261.00
18_F32_F1.5221.00
200_E204_E1.5141.00
94_L107_A1.5121.00
44_K66_Q1.5041.00
67_V94_L1.5021.00
215_L235_L1.4771.00
41_W64_E1.4761.00
211_E214_K1.4681.00
52_I167_L1.4611.00
208_L241_E1.4471.00
10_N40_T1.4311.00
64_E91_Y1.4131.00
41_W44_K1.4071.00
111_R121_V1.4001.00
31_L35_Q1.3931.00
3_L7_T1.3911.00
161_G167_L1.3821.00
18_F24_L1.3681.00
44_K64_E1.3421.00
45_L50_I1.3321.00
226_N229_V1.2991.00
71_E98_E1.2871.00
211_E238_L1.2680.99
18_F85_I1.2660.99
207_K211_E1.2640.99
44_K49_T1.2070.99
171_L174_Q1.2050.99
123_E203_E1.1950.99
7_T50_I1.1890.99
176_I198_R1.1880.99
228_K236_K1.1870.99
205_I242_L1.1830.99
13_I41_W1.1810.99
27_A80_R1.1700.99
106_S127_A1.1430.99
8_E43_Y1.1340.99
94_L171_L1.1330.99
10_N42_L1.1320.99
28_L84_L1.1290.99
24_L28_L1.1200.99
235_L239_K1.1200.99
113_S121_V1.1080.99
213_A216_E1.0870.98
225_F228_K1.0830.98
75_K99_Y1.0800.98
94_L105_L1.0800.98
74_T99_Y1.0740.98
219_L225_F1.0660.98
194_S197_E1.0560.98
45_L171_L1.0440.98
206_E209_E1.0310.98
115_A119_K1.0290.98
35_Q77_L1.0280.98
104_Q130_W1.0260.98
232_N235_L1.0250.98
233_A236_K1.0230.98
212_I242_L1.0180.97
173_R198_R1.0180.97
37_E71_E1.0130.97
62_V165_D1.0060.97
229_V233_A1.0060.97
45_L65_I1.0020.97
224_Q227_R1.0000.97
82_L97_L1.0000.97
231_L235_L0.9900.97
44_K68_F0.9850.97
108_A122_V0.9800.97
205_I209_E0.9780.97
204_E208_L0.9740.97
79_K83_E0.9740.97
26_A29_K0.9680.97
72_L97_L0.9670.97
103_V133_E0.9610.96
219_L232_N0.9600.96
200_E203_E0.9530.96
66_Q89_I0.9520.96
159_P170_N0.9380.96
32_F85_I0.9310.96
190_R193_E0.9300.96
126_F199_V0.9270.96
39_I70_I0.9100.95
205_I212_I0.9090.95
62_V92_P0.9070.95
132_P135_E0.9040.95
41_W91_Y0.9010.95
99_Y102_K0.9000.95
197_E201_R0.8940.95
69_E96_V0.8940.95
64_E89_I0.8930.95
41_W46_A0.8900.94
108_A125_Y0.8870.94
196_E200_E0.8840.94
46_A62_V0.8820.94
45_L164_L0.8800.94
17_K20_E0.8770.94
62_V164_L0.8720.94
107_A172_L0.8710.94
98_E101_D0.8690.94
9_V44_K0.8670.94
112_P120_W0.8650.94
233_A237_K0.8630.94
66_Q93_I0.8530.93
218_K222_E0.8520.93
206_E210_K0.8480.93
213_A217_A0.8470.93
38_R71_E0.8450.93
22_A88_A0.8420.93
197_E200_E0.8420.93
226_N232_N0.8390.93
210_K214_K0.8360.92
234_E238_L0.8310.92
96_V103_V0.8220.92
17_K21_N0.8220.92
219_L229_V0.8190.92
63_P91_Y0.8190.92
161_G170_N0.7980.91
47_P58_A0.7960.90
128_T175_I0.7950.90
11_R14_P0.7900.90
89_I93_I0.7800.90
86_D95_F0.7800.90
80_R83_E0.7760.89
208_L238_L0.7760.89
173_R176_I0.7730.89
44_K86_D0.7730.89
60_R63_P0.7660.89
208_L212_I0.7650.89
70_I107_A0.7600.88
70_I82_L0.7590.88
59_G62_V0.7580.88
176_I199_V0.7570.88
232_N239_K0.7560.88
203_E206_E0.7520.88
228_K233_A0.7510.88
18_F39_I0.7510.88
46_A49_T0.7500.88
97_L104_Q0.7480.88
19_Y29_K0.7480.88
219_L228_K0.7420.87
35_Q76_E0.7360.87
122_V126_F0.7340.87
83_E87_R0.7310.86
46_A66_Q0.7290.86
131_L138_L0.7260.86
46_A64_E0.7250.86
9_V68_F0.7250.86
70_I88_A0.7240.86
232_N236_K0.7210.86
24_L32_F0.7180.85
238_L241_E0.7160.85
162_L166_A0.7050.84
218_K234_E0.7020.84
193_E197_E0.7000.84
169_E173_R0.7000.84
134_D138_L0.6950.83
165_D169_E0.6940.83
40_T71_E0.6890.83
14_P17_K0.6820.82
7_T45_L0.6770.82
12_R16_K0.6760.82
3_L45_L0.6740.82
60_R165_D0.6700.81
6_S49_T0.6620.80
133_E136_L0.6590.80
25_T28_L0.6570.80
214_K217_A0.6550.80
168_Y232_N0.6500.79
22_A27_A0.6500.79
51_N171_L0.6480.79
44_K65_I0.6470.79
12_R40_T0.6460.79
57_D60_R0.6440.79
86_D125_Y0.6430.78
35_Q74_T0.6410.78
180_A201_R0.6400.78
209_E216_E0.6390.78
3_L96_V0.6360.78
77_L81_V0.6350.78
41_W49_T0.6350.78
79_K127_A0.6340.77
47_P50_I0.6320.77
82_L167_L0.6310.77
198_R202_L0.6310.77
216_E220_K0.6240.76
228_K232_N0.6220.76
202_L206_E0.6200.76
203_E210_K0.6190.76
24_L84_L0.6180.76
167_L172_L0.6150.75
59_G163_N0.6140.75
61_E134_D0.6130.75
3_L43_Y0.6130.75
36_I88_A0.6080.75
215_L238_L0.6070.74
124_D199_V0.6030.74
98_E133_E0.6020.74
216_E227_R0.6010.74
8_E42_L0.5910.72
70_I85_I0.5900.72
29_K128_T0.5880.72
85_I127_A0.5870.72
92_P168_Y0.5860.72
11_R16_K0.5860.72
3_L105_L0.5850.72
220_K223_K0.5850.72
46_A91_Y0.5840.72
49_T110_K0.5820.71
95_F114_Q0.5800.71
159_P171_L0.5800.71
98_E103_V0.5770.71
10_N232_N0.5760.71
195_L218_K0.5730.70
235_L238_L0.5710.70
5_K132_P0.5680.69
1_L4_P0.5680.69
204_E211_E0.5670.69
39_I64_E0.5660.69
39_I98_E0.5620.69
106_S198_R0.5610.69
135_E138_L0.5580.68
68_F93_I0.5580.68
19_Y84_L0.5560.68
200_E206_E0.5540.68
65_I164_L0.5530.67
96_V137_R0.5520.67
121_V124_D0.5490.67
116_D121_V0.5490.67
207_K216_E0.5450.66
82_L95_F0.5450.66
32_F125_Y0.5440.66
60_R87_R0.5430.66
32_F43_Y0.5390.65
3_L50_I0.5380.65
192_G227_R0.5360.65
70_I97_L0.5310.64
165_D176_I0.5290.64
11_R110_K0.5290.64
65_I171_L0.5290.64
62_V223_K0.5280.64
83_E124_D0.5270.64
93_I110_K0.5270.64
119_K205_I0.5260.64
132_P137_R0.5260.64
112_P119_K0.5250.63
75_K97_L0.5250.63
2_G45_L0.5250.63
110_K168_Y0.5240.63
17_K125_Y0.5240.63
94_L241_E0.5220.63
66_Q86_D0.5190.63
70_I95_F0.5180.62
90_P93_I0.5180.62
70_I99_Y0.5180.62
6_S36_I0.5140.62
161_G166_A0.5130.62
11_R18_F0.5120.62
140_L167_L0.5110.61
9_V17_K0.5110.61
236_K240_K0.5090.61
97_L130_W0.5060.61
116_D212_I0.5050.60
166_A169_E0.5050.60
179_E191_S0.5040.60
100_E189_L0.5040.60
67_V161_G0.5040.60
189_L192_G0.5030.60
169_E195_L0.5030.60
103_V136_L0.5020.60
28_L80_R0.5010.60
58_A96_V0.5000.60
217_A234_E0.5000.60
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4etpA 1 0.2355 32.1 0.942 Contact Map
1sesA 2 0.3306 25 0.945 Contact Map
4y66A 1 0.4793 24.7 0.945 Contact Map
3onjA 1 0.314 23.2 0.946 Contact Map
1ysmA 1 0.1736 15.9 0.95 Contact Map
4r3zA 1 0.2521 15.5 0.95 Contact Map
2qywA 1 0.3471 13.8 0.951 Contact Map
2dq0A 2 0.3347 12 0.952 Contact Map
2dq3A 2 0.3347 11.6 0.953 Contact Map
4etpB 1 0.2314 11.6 0.953 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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