GREMLIN Database
DUF4386 - Domain of unknown function (DUF4386)
PFAM: PF14329 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 210 (210)
Sequences: 20802 (15312)
Seq/√Len: 1056.6
META: 0.952

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
204_L208_V3.3161.00
70_K74_E3.1731.00
151_Y162_V3.1431.00
41_E45_R3.0391.00
113_A117_Q2.8171.00
82_A85_L2.7601.00
114_P117_Q2.6491.00
112_G116_L2.6091.00
62_A84_R2.2851.00
150_L156_L2.2091.00
112_G115_Q2.1971.00
114_P118_A2.1261.00
115_Q118_A2.1071.00
113_A116_L2.0581.00
76_L145_L2.0471.00
86_V89_A2.0111.00
184_Y187_L2.0051.00
147_G164_G1.9781.00
29_D32_A1.8841.00
111_F115_Q1.8061.00
198_G203_G1.7901.00
198_G202_L1.7801.00
196_A200_L1.7701.00
66_Y78_L1.7631.00
76_L148_Y1.7621.00
2_A64_A1.7331.00
13_G54_G1.6581.00
154_G158_R1.6341.00
84_R142_W1.5961.00
109_A112_G1.5871.00
124_L128_H1.5851.00
74_E78_L1.5781.00
105_L108_A1.5671.00
49_L176_L1.5611.00
150_L161_G1.5581.00
17_E21_P1.5551.00
80_A146_L1.5371.00
35_A38_A1.5121.00
80_A145_L1.5111.00
4_V8_L1.5101.00
188_V192_A1.5091.00
15_F19_Y1.4931.00
179_L182_P1.4551.00
110_A118_A1.4471.00
151_Y158_R1.4371.00
12_A54_G1.4351.00
37_I44_F1.4321.00
84_R87_E1.4301.00
146_L202_L1.4091.00
55_A91_I1.4031.00
63_V82_A1.4001.00
4_V207_L1.3911.00
94_G97_N1.3741.00
73_N76_L1.3731.00
32_A36_N1.3721.00
15_F20_L1.3651.00
42_T46_L1.3611.00
100_A127_L1.3361.00
31_A35_A1.3171.00
198_G205_W1.3171.00
67_P75_G1.3131.00
111_F116_L1.3081.00
143_L164_G1.3071.00
32_A35_A1.3041.00
144_L168_G1.2971.00
151_Y161_G1.2961.00
77_A81_V1.2931.00
116_L119_A1.2911.00
111_F114_P1.2671.00
35_A39_A1.2541.00
67_P78_L1.2451.00
10_F14_I1.2021.00
58_F87_E1.2001.00
58_F84_R1.1821.00
140_G168_G1.1811.00
136_L139_F1.1791.00
67_P70_K1.1691.00
73_N149_L1.1671.00
111_F117_Q1.1661.00
190_L194_P1.1601.00
28_G32_A1.1491.00
190_L193_L1.1491.00
115_Q119_A1.1471.00
123_L126_A1.1251.00
33_T36_N1.1231.00
147_G161_G1.1151.00
5_L64_A1.1021.00
160_L201_S1.1011.00
112_G117_Q1.0991.00
165_L169_V1.0951.00
36_N39_A1.0881.00
110_A114_P1.0871.00
118_A121_A1.0721.00
8_L57_A1.0711.00
58_F91_I1.0701.00
66_Y74_E1.0681.00
119_A122_L1.0591.00
55_A92_A1.0571.00
151_Y165_L1.0561.00
8_L12_A1.0521.00
142_W146_L1.0511.00
117_Q121_A1.0481.00
193_L197_L1.0401.00
14_I18_L1.0391.00
147_G165_L1.0381.00
36_N40_H1.0371.00
128_H132_F1.0251.00
41_E106_Q1.0241.00
147_G168_G1.0201.00
116_L120_L1.0201.00
126_A129_D1.0181.00
203_G207_L1.0161.00
21_P51_E1.0081.00
109_A114_P1.0081.00
101_A105_L1.0021.00
197_L201_S1.0021.00
9_T57_A1.0011.00
103_T106_Q1.0001.00
72_V149_L0.9951.00
194_P197_L0.9901.00
58_F88_A0.9841.00
192_A195_A0.9811.00
112_G119_A0.9721.00
123_L127_L0.9661.00
66_Y77_A0.9581.00
33_T37_I0.9541.00
30_A34_A0.9491.00
96_L131_G0.9471.00
51_E91_I0.9441.00
20_L23_P0.9421.00
117_Q120_L0.9411.00
191_L195_A0.9401.00
63_V79_L0.9331.00
54_G57_A0.9321.00
169_V173_V0.9321.00
111_F118_A0.9311.00
71_P74_E0.9241.00
103_T130_W0.9241.00
12_A50_L0.9241.00
201_S205_W0.9211.00
13_G16_A0.9121.00
66_Y70_K0.9111.00
19_Y23_P0.9041.00
171_Y174_G0.9041.00
54_G58_F0.9011.00
122_L126_A0.8991.00
48_I99_L0.8971.00
167_G171_Y0.8961.00
83_F141_L0.8911.00
69_L149_L0.8841.00
14_I17_E0.8841.00
167_G170_L0.8671.00
162_V166_I0.8631.00
159_W162_V0.8451.00
55_A88_A0.8421.00
188_V191_L0.8411.00
130_W133_L0.8341.00
34_A38_A0.8341.00
141_L145_L0.8291.00
72_V153_S0.8281.00
11_V14_I0.8281.00
13_G17_E0.8251.00
109_A117_Q0.8231.00
112_G118_A0.8211.00
51_E95_V0.8201.00
110_A119_A0.8181.00
193_L196_A0.8181.00
97_N101_A0.8151.00
2_A65_L0.8141.00
159_W201_S0.8121.00
94_G131_G0.8101.00
159_W163_L0.8081.00
95_V98_L0.8051.00
199_E202_L0.8041.00
98_L128_H0.8041.00
189_P192_A0.8041.00
118_A122_L0.8041.00
77_A149_L0.8031.00
79_L83_F0.7971.00
67_P74_E0.7961.00
7_L10_F0.7941.00
44_F48_I0.7901.00
5_L60_G0.7881.00
184_Y188_V0.7821.00
94_G135_G0.7821.00
173_V177_A0.7781.00
62_A88_A0.7771.00
26_V33_T0.7771.00
195_A198_G0.7751.00
21_P25_I0.7751.00
10_F203_G0.7741.00
170_L195_A0.7631.00
17_E22_G0.7621.00
46_L50_L0.7601.00
114_P119_A0.7581.00
66_Y81_V0.7551.00
179_L183_D0.7531.00
21_P24_L0.7521.00
195_A203_G0.7501.00
91_I135_G0.7451.00
110_A117_Q0.7431.00
26_V29_D0.7421.00
59_I86_V0.7411.00
140_G172_L0.7401.00
150_L202_L0.7351.00
76_L152_R0.7341.00
15_F46_L0.7301.00
10_F13_G0.7301.00
80_A142_W0.7301.00
34_A37_I0.7261.00
187_L191_L0.7211.00
87_E91_I0.7191.00
132_F136_L0.7131.00
48_I95_V0.7131.00
148_Y152_R0.7131.00
63_V75_G0.7121.00
97_N124_L0.7101.00
186_A190_L0.7071.00
180_L183_D0.7071.00
113_A118_A0.7001.00
129_D133_L0.6991.00
38_A105_L0.6991.00
60_G64_A0.6981.00
6_Y9_T0.6971.00
142_W145_L0.6971.00
42_T45_R0.6881.00
18_L22_G0.6861.00
81_V85_L0.6851.00
26_V36_N0.6811.00
143_L147_G0.6811.00
65_L146_L0.6801.00
171_Y195_A0.6781.00
157_P160_L0.6781.00
200_L203_G0.6761.00
51_E128_H0.6701.00
125_L129_D0.6691.00
59_I88_A0.6691.00
117_Q122_L0.6681.00
83_F145_L0.6611.00
100_A124_L0.6581.00
109_A113_A0.6511.00
165_L168_G0.6461.00
38_A41_E0.6451.00
202_L206_L0.6451.00
200_L204_L0.6431.00
150_L164_G0.6431.00
99_L103_T0.6421.00
53_I57_A0.6411.00
143_L168_G0.6381.00
185_S188_V0.6351.00
54_G91_I0.6321.00
199_E203_G0.6301.00
109_A116_L0.6301.00
127_L130_W0.6291.00
94_G128_H0.6241.00
124_L127_L0.6231.00
96_L99_L0.6161.00
76_L149_L0.6131.00
168_G171_Y0.6131.00
191_L194_P0.6091.00
195_A199_E0.6081.00
198_G201_S0.6081.00
72_V155_L0.6071.00
62_A142_W0.6031.00
194_P201_S0.6001.00
51_E54_G0.5981.00
67_P71_P0.5981.00
144_L165_L0.5961.00
48_I98_L0.5941.00
110_A113_A0.5931.00
189_P193_L0.5881.00
3_G6_Y0.5871.00
192_A196_A0.5861.00
9_T13_G0.5791.00
80_A150_L0.5781.00
60_G79_L0.5771.00
80_A84_R0.5761.00
89_A92_A0.5751.00
204_L209_K0.5751.00
75_G79_L0.5751.00
204_L207_L0.5721.00
195_A201_S0.5701.00
135_G139_F0.5671.00
16_A50_L0.5641.00
166_I169_V0.5601.00
40_H43_L0.5561.00
146_L150_L0.5561.00
6_Y10_F0.5541.00
167_G196_A0.5501.00
133_L178_V0.5461.00
56_L60_G0.5411.00
58_F62_A0.5411.00
69_L156_L0.5401.00
23_P43_L0.5381.00
174_G178_V0.5371.00
132_F135_G0.5361.00
65_L80_A0.5341.00
14_I19_Y0.5311.00
8_L53_I0.5311.00
87_E135_G0.5231.00
168_G195_A0.5231.00
51_E132_F0.5171.00
196_A202_L0.5161.00
167_G195_A0.5161.00
186_A189_P0.5161.00
156_L202_L0.5161.00
16_A19_Y0.5151.00
59_I85_L0.5131.00
39_A42_T0.5131.00
134_L137_I0.5121.00
194_P198_G0.5121.00
147_G163_L0.5061.00
41_E44_F0.5031.00
163_L201_S0.5021.00
97_N100_A0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4p79A 1 0.3095 17.3 0.904 Contact Map
3wu2J 1 0.1857 7.6 0.918 Contact Map
2jafA 3 0.7476 5.5 0.923 Contact Map
4o6yA 2 0.8667 4.6 0.926 Contact Map
2xq2A 2 0.4381 4.5 0.926 Contact Map
3ux4A 3 0.6619 4.1 0.928 Contact Map
4c7rA 3 0.9476 4 0.928 Contact Map
3wfdB 1 0.6476 3.8 0.929 Contact Map
4ymuD 2 0.319 2.9 0.933 Contact Map
4djkA 2 0.9238 2.8 0.934 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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