GREMLIN Database
DUF4366 - Domain of unknown function (DUF4366)
PFAM: PF14283 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 238 (222)
Sequences: 1116 (833)
Seq/√Len: 55.9
META: 0.823

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
78_V88_L4.5341.00
94_R99_E3.3101.00
201_Y205_Y3.0861.00
222_Y225_D2.7901.00
185_L189_L2.4191.00
183_G187_L2.3961.00
201_Y204_I2.3921.00
55_L73_K2.2861.00
182_G186_L2.2821.00
24_G27_T2.2501.00
84_N109_E2.2121.00
66_D76_I2.1941.00
144_N147_E2.0971.00
206_K210_E2.0931.00
66_D74_Q2.0051.00
82_S109_E1.9801.00
74_Q94_R1.9691.00
61_G78_V1.9561.00
205_Y209_K1.9181.00
81_K84_N1.8911.00
184_L188_V1.8190.99
129_C148_C1.7900.99
60_N108_D1.7860.99
81_K108_D1.7550.99
102_Y106_A1.7500.99
32_P36_E1.6860.99
76_I88_L1.6810.99
184_L187_L1.6590.99
60_N63_L1.6540.99
67_I71_D1.6270.99
76_I94_R1.6200.99
105_N114_A1.6170.99
90_I103_F1.6110.98
160_T163_P1.6020.98
94_R100_N1.5840.98
57_P64_V1.5810.98
57_P105_N1.5780.98
109_E116_A1.5700.98
201_Y207_P1.5670.98
105_N115_L1.5650.98
99_E102_Y1.5620.98
138_G141_N1.5480.98
96_G100_N1.5410.98
55_L67_I1.5020.98
57_P62_T1.4920.97
84_N105_N1.4810.97
157_P160_T1.4780.97
197_G201_Y1.4780.97
200_Y205_Y1.4650.97
60_N116_A1.4610.97
76_I90_I1.4530.97
79_T85_V1.4480.97
202_F205_Y1.4410.97
231_E234_E1.4270.97
81_K106_A1.4190.97
60_N106_A1.4160.97
72_G95_D1.4130.97
6_L11_L1.4100.96
34_T37_T1.4000.96
61_G102_Y1.3870.96
109_E113_L1.3680.96
171_P174_K1.3550.96
11_L14_A1.3360.95
74_Q83_G1.3330.95
35_E38_P1.3300.95
60_N81_K1.3140.95
67_I76_I1.2980.94
180_S183_G1.2920.94
66_D94_R1.2850.94
9_L14_A1.2790.94
65_D73_K1.2720.94
82_S114_A1.2530.93
103_F115_L1.2280.93
42_E45_T1.2230.92
120_E123_E1.2190.92
204_I207_P1.2190.92
54_P73_K1.2160.92
26_P30_T1.2130.92
106_A116_A1.2110.92
39_E43_T1.2100.92
217_D220_Y1.2090.92
56_T73_K1.1690.91
141_N148_C1.1670.90
75_F89_I1.1600.90
221_D228_D1.1500.90
7_A10_L1.1500.90
63_L66_D1.1490.90
103_F107_V1.1470.90
8_A11_L1.1460.90
230_E233_E1.1420.90
220_Y223_D1.1400.89
87_Y103_F1.1250.89
63_L85_V1.1220.89
190_A194_I1.1190.89
61_G96_G1.1150.88
26_P29_E1.1100.88
219_D222_Y1.1090.88
71_D99_E1.1060.88
6_L10_L1.1050.88
129_C138_G1.0910.87
145_M148_C1.0880.87
105_N108_D1.0850.87
185_L188_V1.0820.87
37_T41_E1.0780.87
76_I108_D1.0640.86
118_D121_E1.0630.86
166_E170_E1.0610.86
8_A13_G1.0600.86
57_P72_G1.0540.85
174_K177_K1.0450.85
25_E31_E1.0450.85
63_L81_K1.0420.85
182_G189_L1.0400.85
60_N66_D1.0350.84
231_E235_E1.0330.84
199_G205_Y1.0270.84
10_L13_G1.0270.84
89_I99_E1.0250.84
14_A20_F1.0150.83
81_K116_A1.0130.83
33_V36_E1.0010.82
63_L108_D1.0000.82
110_A113_L0.9970.82
64_V101_V0.9970.82
60_N78_V0.9960.82
201_Y206_K0.9940.82
108_D116_A0.9850.81
68_T71_D0.9840.81
175_K178_G0.9790.81
38_P44_E0.9780.81
85_V108_D0.9740.80
64_V79_T0.9670.80
23_G26_P0.9620.80
143_N146_T0.9610.80
209_K213_G0.9480.79
114_A117_E0.9440.78
57_P63_L0.9430.78
73_K91_D0.9410.78
198_A202_F0.9390.78
213_G217_D0.9380.78
129_C141_N0.9320.77
79_T99_E0.9270.77
183_G193_G0.9260.77
76_I96_G0.9170.76
63_L106_A0.9120.76
200_Y204_I0.9080.76
181_G184_L0.9080.76
138_G148_C0.9080.76
8_A14_A0.9060.75
186_L193_G0.9060.75
230_E234_E0.8940.74
148_C151_K0.8890.74
55_L85_V0.8890.74
61_G116_A0.8870.74
37_T40_E0.8870.74
33_V37_T0.8860.74
64_V85_V0.8860.74
96_G108_D0.8830.74
173_K176_E0.8820.73
189_L192_A0.8810.73
103_F114_A0.8800.73
74_Q81_K0.8700.72
25_E28_E0.8680.72
31_E34_T0.8660.72
34_T38_P0.8640.72
82_S113_L0.8640.72
153_P161_E0.8550.71
55_L65_D0.8520.71
38_P41_E0.8510.71
110_A119_D0.8490.71
28_E33_V0.8480.70
7_A20_F0.8470.70
6_L13_G0.8410.70
183_G186_L0.8410.70
220_Y224_E0.8410.70
61_G106_A0.8370.69
211_A215_D0.8360.69
29_E35_E0.8240.68
94_R97_E0.8240.68
110_A114_A0.8240.68
64_V67_I0.8230.68
25_E30_T0.8170.68
95_D99_E0.8160.68
34_T39_E0.8120.67
233_E236_D0.8090.67
8_A12_M0.8080.67
61_G66_D0.8050.66
36_E39_E0.8050.66
63_L101_V0.7970.66
12_M15_F0.7960.66
3_A10_L0.7950.65
159_P163_P0.7910.65
232_E235_E0.7900.65
207_P211_A0.7880.65
4_A7_A0.7870.65
84_N115_L0.7870.65
54_P65_D0.7860.65
105_N110_A0.7850.65
61_G75_F0.7820.64
81_K115_L0.7800.64
188_V192_A0.7780.64
156_E163_P0.7760.64
53_E67_I0.7750.64
4_A9_L0.7750.64
80_T116_A0.7710.63
199_G204_I0.7670.63
84_N106_A0.7650.62
56_T65_D0.7640.62
63_L78_V0.7620.62
123_E126_P0.7590.62
41_E44_E0.7590.62
82_S96_G0.7550.61
66_D101_V0.7500.61
4_A11_L0.7490.61
35_E39_E0.7470.61
113_L116_A0.7470.61
10_L14_A0.7340.59
32_P35_E0.7330.59
144_N148_C0.7280.59
78_V108_D0.7210.58
55_L64_V0.7160.57
129_C150_G0.7140.57
181_G185_L0.7120.57
26_P37_T0.7100.57
89_I113_L0.7090.57
57_P115_L0.7080.57
65_D77_T0.7030.56
95_D100_N0.7030.56
76_I103_F0.7020.56
24_G28_E0.6920.55
57_P96_G0.6920.55
34_T45_T0.6920.55
217_D223_D0.6910.55
189_L193_G0.6840.54
9_L13_G0.6810.54
11_L15_F0.6810.54
22_G26_P0.6780.53
45_T48_E0.6770.53
183_G190_A0.6730.53
55_L61_G0.6710.53
120_E126_P0.6680.52
141_N145_M0.6670.52
20_F23_G0.6620.52
91_D102_Y0.6550.51
66_D81_K0.6500.50
36_E41_E0.6500.50
85_V106_A0.6490.50
185_L193_G0.6440.50
56_T59_G0.6430.50
40_E44_E0.6420.49
71_D80_T0.6420.49
71_D96_G0.6410.49
62_T100_N0.6410.49
210_E215_D0.6400.49
84_N114_A0.6390.49
85_V116_A0.6370.49
129_C140_V0.6330.48
185_L197_G0.6330.48
67_I101_V0.6320.48
64_V102_Y0.6300.48
38_P43_T0.6280.48
83_G109_E0.6270.48
83_G107_V0.6260.48
67_I96_G0.6250.48
63_L71_D0.6240.47
68_T89_I0.6230.47
27_T30_T0.6180.47
71_D101_V0.6170.47
89_I102_Y0.6160.47
27_T33_V0.6150.47
54_P67_I0.6100.46
72_G101_V0.6080.46
76_I106_A0.6040.45
28_E31_E0.6040.45
56_T105_N0.6000.45
223_D229_E0.6000.45
7_A16_S0.6000.45
85_V102_Y0.5980.45
76_I81_K0.5930.44
190_A193_G0.5870.44
82_S115_L0.5870.44
79_T102_Y0.5840.43
222_Y229_E0.5830.43
31_E37_T0.5810.43
174_K179_G0.5800.43
61_G67_I0.5790.43
182_G185_L0.5790.43
142_A145_M0.5770.42
98_E101_V0.5760.42
122_E125_P0.5680.41
31_E41_E0.5680.41
7_A11_L0.5670.41
36_E50_E0.5650.41
210_E222_Y0.5640.41
36_E52_E0.5630.41
189_L196_G0.5630.41
142_A148_C0.5620.41
59_G64_V0.5610.41
227_D234_E0.5580.40
19_A43_T0.5580.40
96_G106_A0.5550.40
60_N96_G0.5550.40
31_E35_E0.5540.40
204_I208_K0.5540.40
153_P171_P0.5500.40
75_F79_T0.5480.39
5_L10_L0.5470.39
89_I104_L0.5460.39
47_E51_E0.5460.39
94_R102_Y0.5440.39
63_L96_G0.5430.39
67_I72_G0.5420.39
59_G100_N0.5410.39
99_E106_A0.5390.38
225_D229_E0.5380.38
71_D94_R0.5350.38
72_G94_R0.5340.38
161_E171_P0.5300.38
16_S22_G0.5270.37
129_C145_M0.5240.37
43_T48_E0.5230.37
117_E120_E0.5230.37
78_V81_K0.5180.36
61_G115_L0.5170.36
186_L189_L0.5160.36
96_G102_Y0.5110.36
113_L119_D0.5100.36
61_G80_T0.5090.35
191_L194_I0.5090.35
201_Y209_K0.5090.35
71_D95_D0.5060.35
124_E127_A0.5010.35
54_P91_D0.5000.35
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2l2tA 2 0.1849 70.2 0.935 Contact Map
2ks1B 1 0.1849 58.2 0.94 Contact Map
2jwaA 2 0.1849 56.4 0.94 Contact Map
4u7aA 1 0 55.8 0.941 Contact Map
2rddB 1 0.1555 36.7 0.947 Contact Map
2micA 2 0.1723 33.5 0.948 Contact Map
2m20A 2 0.2395 28.6 0.95 Contact Map
2kncA 1 0.2143 26.8 0.951 Contact Map
1iijA 1 0.1471 26.5 0.951 Contact Map
2k1kA 2 0.1555 23.9 0.952 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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