GREMLIN Database
DUF4349 - Domain of unknown function (DUF4349)
PFAM: PF14257 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 265 (251)
Sequences: 15947 (13338)
Seq/√Len: 841.9
META: 0.885

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
167_E171_A4.0791.00
150_R187_E3.9631.00
120_R128_E3.2501.00
175_E178_R3.2091.00
153_E180_R2.9031.00
111_R114_A2.8001.00
67_D70_A2.6921.00
82_L115_A2.6181.00
165_K168_D2.5421.00
55_I132_A2.5111.00
158_E161_E2.4171.00
59_A112_F2.3281.00
81_A118_E2.3091.00
155_R173_E2.2011.00
57_R113_D2.0671.00
138_Q189_Q1.9421.00
178_R182_E1.9231.00
153_E157_L1.9201.00
112_F130_I1.9181.00
251_P255_L1.9091.00
54_K139_Y1.9091.00
82_L118_E1.8831.00
78_L118_E1.8811.00
146_L183_I1.8641.00
64_E126_L1.8611.00
77_A81_A1.8371.00
153_E176_L1.8351.00
149_L182_E1.8261.00
143_E147_K1.8011.00
137_E189_Q1.7981.00
54_K194_D1.7771.00
104_T203_T1.7651.00
70_A74_A1.7571.00
155_R158_E1.7561.00
187_E191_R1.7411.00
143_E190_L1.7011.00
82_L111_R1.6721.00
135_V193_L1.6671.00
102_T205_T1.6661.00
159_L169_I1.6641.00
53_R134_D1.6581.00
71_A206_L1.6401.00
190_L194_D1.6371.00
188_G192_Y1.6191.00
115_A202_I1.6111.00
75_I204_V1.6011.00
78_L82_L1.5731.00
62_S207_Y1.5571.00
68_V101_A1.4921.00
181_G185_S1.4821.00
64_E209_P1.4801.00
78_L115_A1.4711.00
74_A77_A1.4561.00
159_L172_V1.4541.00
62_S127_S1.4421.00
156_L179_V1.4271.00
191_R195_D1.4241.00
59_A202_I1.4181.00
72_A76_R1.4161.00
126_L207_Y1.3961.00
241_G245_F1.3911.00
54_K197_V1.3751.00
145_R184_E1.3691.00
249_L253_L1.3611.00
152_T177_S1.3561.00
59_A116_L1.3551.00
90_N129_S1.3511.00
68_V208_E1.3391.00
75_I206_L1.3361.00
235_L239_L1.3311.00
62_S205_T1.3251.00
152_T179_V1.3241.00
79_A105_L1.3171.00
245_F248_A1.2961.00
75_I119_L1.2741.00
182_E185_S1.2711.00
154_A158_E1.2591.00
148_N157_L1.2581.00
225_G228_L1.2431.00
73_A77_A1.2351.00
76_R80_E1.2341.00
146_L186_L1.2321.00
248_A251_P1.2271.00
192_Y196_Q1.2121.00
143_E194_D1.2061.00
142_L186_L1.2061.00
171_A174_R1.1941.00
153_E183_I1.1921.00
56_I196_Q1.1881.00
139_Y194_D1.1851.00
64_E124_K1.1761.00
104_T201_T1.1721.00
78_L81_A1.1711.00
166_V169_I1.1671.00
133_E138_Q1.1651.00
72_A101_A1.1601.00
236_V240_A1.1561.00
116_L128_E1.1561.00
99_R208_E1.1561.00
244_I247_G1.1481.00
146_L150_R1.1461.00
118_E121_G1.1341.00
220_F223_R1.1341.00
145_R182_E1.1121.00
56_I135_V1.1111.00
154_A157_L1.1071.00
84_G108_P1.1051.00
114_A118_E1.1031.00
107_V115_A1.0991.00
76_R89_S1.0991.00
147_K151_A1.0981.00
69_D99_R1.0921.00
65_V206_L1.0921.00
151_A154_A1.0861.00
166_V172_V1.0851.00
232_W236_V1.0821.00
220_F224_I1.0801.00
221_G224_I1.0771.00
87_A106_R1.0661.00
259_I263_V1.0651.00
256_L260_L1.0621.00
85_Y199_Y1.0581.00
88_S104_T1.0551.00
58_T133_E1.0521.00
227_A231_G1.0501.00
77_A80_E1.0401.00
151_A157_L1.0381.00
58_T131_S1.0361.00
225_G229_R1.0301.00
60_S203_T1.0301.00
140_V192_Y1.0291.00
149_L152_T1.0231.00
238_F241_G1.0181.00
105_L204_V1.0081.00
139_Y190_L1.0051.00
115_A119_L1.0051.00
221_G225_G1.0041.00
32_A35_P1.0031.00
258_L262_L0.9991.00
80_E113_D0.9941.00
244_I248_A0.9941.00
71_A122_L0.9931.00
252_F256_L0.9841.00
241_G244_I0.9841.00
228_L232_W0.9701.00
112_F202_I0.9691.00
60_S129_S0.9661.00
224_I228_L0.9631.00
65_V71_A0.9601.00
238_F242_L0.9581.00
250_L253_L0.9561.00
82_L114_A0.9551.00
238_F252_F0.9491.00
57_R130_I0.9451.00
245_F249_L0.9401.00
237_A240_A0.9361.00
153_E179_V0.9351.00
237_A241_G0.9251.00
142_L190_L0.9231.00
62_S126_L0.9201.00
145_R181_G0.9161.00
113_D117_A0.9111.00
112_F200_S0.9091.00
189_Q193_L0.9011.00
60_S205_T0.9011.00
73_A76_R0.8991.00
24_A27_A0.8941.00
174_R178_R0.8901.00
177_S181_G0.8871.00
100_S205_T0.8841.00
258_L261_W0.8831.00
147_K150_R0.8811.00
17_A20_A0.8751.00
234_A238_F0.8741.00
63_L123_G0.8711.00
148_N181_G0.8691.00
223_R240_A0.8681.00
69_D73_A0.8681.00
227_A248_A0.8641.00
142_L189_Q0.8631.00
236_V239_L0.8591.00
173_E177_S0.8541.00
70_A73_A0.8541.00
137_E192_Y0.8531.00
21_A24_A0.8471.00
102_T203_T0.8461.00
75_I79_A0.8441.00
59_A130_I0.8431.00
148_N180_R0.8431.00
29_A32_A0.8421.00
156_L176_L0.8401.00
255_L259_I0.8351.00
160_L172_V0.8341.00
53_R136_T0.8321.00
63_L71_A0.8321.00
220_F240_A0.8291.00
72_A103_L0.8281.00
150_R153_E0.8261.00
180_R184_E0.8261.00
146_L190_L0.8151.00
54_K190_L0.8111.00
105_L115_A0.8091.00
113_D130_I0.8091.00
63_L125_V0.8071.00
109_A197_V0.8061.00
78_L122_L0.8061.00
61_L116_L0.8051.00
256_L259_I0.7941.00
56_I133_E0.7911.00
184_E187_E0.7901.00
33_E36_A0.7891.00
149_L186_L0.7891.00
158_E162_K0.7871.00
230_D234_A0.7851.00
57_R112_F0.7841.00
224_I227_A0.7831.00
106_R201_T0.7811.00
79_A84_G0.7791.00
18_S21_A0.7751.00
13_A16_P0.7731.00
179_V183_I0.7721.00
255_L258_L0.7711.00
100_S207_Y0.7651.00
233_R237_A0.7621.00
191_R194_D0.7601.00
73_A117_A0.7591.00
137_E188_G0.7581.00
114_A117_A0.7511.00
171_A175_E0.7481.00
223_R226_D0.7461.00
117_A120_R0.7461.00
137_E191_R0.7441.00
14_S17_A0.7421.00
229_R233_R0.7411.00
159_L166_V0.7401.00
30_A33_E0.7381.00
223_R227_A0.7371.00
19_A22_A0.7361.00
231_G235_L0.7351.00
57_R132_A0.7311.00
223_R231_G0.7291.00
68_V99_R0.7211.00
125_V128_E0.7181.00
227_A244_I0.7171.00
60_S131_S0.7151.00
28_A31_A0.7151.00
145_R185_S0.7121.00
160_L169_I0.7111.00
239_L243_L0.7031.00
75_I103_L0.7031.00
25_A28_A0.7031.00
83_G111_R0.7011.00
157_L180_R0.6961.00
149_L179_V0.6951.00
27_A30_A0.6941.00
178_R181_G0.6931.00
88_S131_S0.6931.00
144_A148_N0.6911.00
116_L202_I0.6901.00
174_R177_S0.6881.00
20_A23_A0.6871.00
22_A25_A0.6811.00
75_I122_L0.6781.00
152_T175_E0.6741.00
157_L176_L0.6681.00
58_T203_T0.6661.00
231_G240_A0.6651.00
222_S226_D0.6651.00
36_A39_A0.6641.00
71_A74_A0.6611.00
148_N151_A0.6611.00
60_S127_S0.6601.00
148_N152_T0.6571.00
140_V144_A0.6531.00
23_A26_A0.6511.00
61_L128_E0.6491.00
253_L257_A0.6461.00
168_D171_A0.6431.00
39_A42_S0.6431.00
146_L149_L0.6341.00
152_T173_E0.6341.00
251_P254_L0.6341.00
16_P19_A0.6321.00
220_F225_G0.6311.00
61_L202_I0.6311.00
249_L252_F0.6311.00
63_L206_L0.6301.00
257_A261_W0.6291.00
106_R195_D0.6201.00
26_A29_A0.6191.00
76_R86_V0.6181.00
35_P38_A0.6171.00
119_L204_V0.6121.00
156_L177_S0.6091.00
231_G236_V0.6081.00
37_A40_A0.6041.00
164_G168_D0.6041.00
89_S130_I0.6031.00
54_K198_A0.6021.00
136_T140_V0.6011.00
116_L120_R0.6011.00
66_K209_P0.6001.00
76_R113_D0.6001.00
234_A248_A0.6001.00
74_A122_L0.5991.00
12_A16_P0.5981.00
138_Q193_L0.5971.00
79_A204_V0.5951.00
18_S22_A0.5931.00
135_V197_V0.5911.00
227_A247_G0.5881.00
156_L172_V0.5871.00
53_R85_Y0.5851.00
232_W235_L0.5851.00
230_D244_I0.5841.00
160_L176_L0.5841.00
216_P219_G0.5821.00
146_L187_E0.5791.00
76_R79_A0.5791.00
233_R236_V0.5791.00
86_V105_L0.5781.00
250_L254_L0.5751.00
56_I197_V0.5731.00
75_I105_L0.5731.00
224_I235_L0.5701.00
234_A241_G0.5691.00
71_A75_I0.5631.00
155_R159_L0.5621.00
79_A86_V0.5611.00
66_K123_G0.5601.00
187_E190_L0.5591.00
238_F245_F0.5551.00
123_G126_L0.5551.00
31_A34_A0.5551.00
59_A200_S0.5521.00
188_G191_R0.5521.00
211_A214_P0.5511.00
87_A104_T0.5501.00
15_A18_S0.5501.00
226_D230_D0.5481.00
147_K157_L0.5481.00
156_L166_V0.5471.00
149_L177_S0.5461.00
172_V175_E0.5461.00
74_A78_L0.5451.00
86_V89_S0.5451.00
139_Y143_E0.5431.00
224_I243_L0.5431.00
58_T196_Q0.5421.00
147_K153_E0.5411.00
150_R154_A0.5411.00
224_I247_G0.5331.00
89_S101_A0.5321.00
147_K154_A0.5291.00
64_E207_Y0.5291.00
162_K166_V0.5271.00
94_G99_R0.5221.00
152_T180_R0.5221.00
231_G248_A0.5191.00
149_L181_G0.5181.00
166_V170_L0.5181.00
152_T169_I0.5161.00
186_L190_L0.5131.00
58_T201_T0.5111.00
155_R161_E0.5101.00
61_L119_L0.5101.00
154_A161_E0.5101.00
67_D71_A0.5091.00
240_A243_L0.5091.00
72_A89_S0.5091.00
227_A230_D0.5081.00
189_Q192_Y0.5081.00
87_A201_T0.5061.00
222_S225_G0.5041.00
165_K169_I0.5031.00
193_L196_Q0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2ko1A 2 0.3283 56.4 0.923 Contact Map
1zpvA 3 0.3283 54.1 0.924 Contact Map
4y66B 1 0.683 35.8 0.932 Contact Map
2f1mA 2 0.6755 35 0.932 Contact Map
2lvwA 2 0.366 32.9 0.934 Contact Map
3j9tb 1 0.5736 31.8 0.934 Contact Map
4tkoB 1 0.7094 29.7 0.935 Contact Map
3lh2S 2 0.2604 27.9 0.936 Contact Map
1vf7A 5 0.6453 25.6 0.937 Contact Map
3fppA 3 0.6981 24 0.938 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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