GREMLIN Database
DUF4335 - Domain of unknown function (DUF4335)
PFAM: PF14233 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 188 (181)
Sequences: 630 (433)
Seq/√Len: 32.2
META: 0.649

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
52_R56_E5.7241.00
65_Y100_H4.8651.00
55_L133_L3.2931.00
160_P164_R3.2921.00
66_A127_V3.1221.00
101_R117_K2.9541.00
155_L158_K2.8211.00
67_Q141_P2.7041.00
179_A183_F2.5391.00
95_D172_I2.4891.00
43_G132_Q2.4720.99
2_R11_E2.4450.99
169_W181_A2.3350.99
5_L129_A2.2870.99
171_G179_A2.2870.99
100_H123_L2.1280.98
131_D136_D2.1270.98
41_L129_A2.0530.98
16_P53_E2.0260.98
37_W54_H1.9990.98
34_L56_E1.9570.97
3_Y10_L1.9090.97
109_P113_P1.9050.97
11_E40_R1.8270.96
16_P131_D1.7730.95
150_L154_Y1.7720.95
170_I173_A1.7040.94
97_L117_K1.7030.94
180_V183_F1.7000.94
55_L134_N1.6820.94
37_W46_P1.6810.94
177_L180_V1.6340.93
148_Q152_R1.6170.92
166_I170_I1.5670.91
43_G122_Q1.5630.91
73_V158_K1.5530.91
1_R6_P1.5480.90
71_S171_G1.5440.90
162_A182_L1.5210.90
174_S182_L1.5010.89
5_L37_W1.4400.87
71_S136_D1.4080.86
101_R115_V1.3710.84
1_R8_C1.3470.83
11_E162_A1.3420.83
171_G178_A1.3290.82
148_Q154_Y1.3240.82
116_I161_L1.3130.81
153_R157_N1.2930.80
4_T9_T1.2870.80
80_G83_S1.2670.79
76_P79_S1.2650.79
12_L40_R1.2530.78
3_Y136_D1.2230.76
26_E32_S1.2120.76
1_R5_L1.1980.75
9_T133_L1.1760.73
71_S120_T1.1630.73
62_V126_L1.1310.70
149_P154_Y1.1310.70
8_C34_L1.1250.70
22_A25_N1.1170.69
83_S86_L1.1160.69
151_S154_Y1.0930.68
16_P23_S1.0850.67
79_S89_L1.0760.67
65_Y123_L1.0700.66
35_S143_L1.0670.66
56_E144_S1.0660.66
179_A185_L1.0590.65
2_R29_D1.0550.65
18_P26_E1.0540.65
48_L160_P1.0530.65
17_D33_I1.0490.65
18_P141_P1.0490.65
139_T143_L1.0490.65
172_I179_A1.0410.64
108_K113_P1.0380.64
134_N137_S1.0360.64
61_A161_L1.0300.63
50_G58_L1.0200.62
121_V168_P1.0150.62
6_P139_T1.0110.62
68_E91_P1.0070.61
50_G55_L1.0030.61
3_Y12_L0.9990.61
14_G17_D0.9930.60
136_D171_G0.9810.59
6_P152_R0.9780.59
120_T171_G0.9760.59
6_P129_A0.9730.59
2_R9_T0.9640.58
138_Q184_L0.9560.57
23_S106_S0.9550.57
15_L51_G0.9530.57
79_S82_E0.9500.57
102_L123_L0.9470.57
23_S177_L0.9420.56
9_T119_S0.9310.55
14_G113_P0.9230.55
59_M128_E0.9220.54
132_Q175_L0.9190.54
102_L116_I0.9170.54
64_I114_L0.9050.53
60_D166_I0.9030.53
150_L153_R0.8990.53
14_G143_L0.8990.53
6_P140_L0.8980.53
4_T37_W0.8970.52
88_K96_N0.8930.52
68_E79_S0.8930.52
118_L122_Q0.8860.52
147_L180_V0.8780.51
8_C69_L0.8690.50
173_A179_A0.8600.49
34_L55_L0.8570.49
121_V167_P0.8480.48
113_P130_L0.8420.48
101_R142_D0.8310.47
82_E176_A0.8290.47
5_L39_L0.8270.47
59_M63_S0.8250.47
15_L33_I0.8230.46
8_C129_A0.8190.46
80_G84_E0.8160.46
150_L155_L0.8110.45
120_T132_Q0.8100.45
121_V128_E0.8090.45
24_N74_P0.8070.45
154_Y157_N0.8060.45
18_P41_L0.8060.45
71_S164_R0.8050.45
82_E93_K0.8000.44
171_G184_L0.7930.44
30_L157_N0.7920.44
118_L126_L0.7870.43
89_L128_E0.7860.43
64_I91_P0.7740.42
88_K180_V0.7720.42
67_Q136_D0.7710.42
43_G153_R0.7700.42
8_C143_L0.7680.42
91_P96_N0.7640.42
153_R156_N0.7620.41
51_G129_A0.7610.41
78_E96_N0.7610.41
59_M133_L0.7600.41
175_L178_A0.7580.41
171_G186_P0.7570.41
6_P141_P0.7560.41
34_L181_A0.7550.41
148_Q157_N0.7540.41
58_L130_L0.7520.41
120_T178_A0.7520.41
71_S186_P0.7510.41
16_P33_I0.7480.40
4_T8_C0.7480.40
160_P166_I0.7460.40
92_I172_I0.7310.39
4_T116_I0.7280.39
112_K115_V0.7260.39
45_K101_R0.7240.38
7_S39_L0.7230.38
22_A111_V0.7220.38
41_L46_P0.7220.38
143_L155_L0.7150.38
166_I185_L0.7110.37
116_I181_A0.7100.37
23_S49_E0.7060.37
67_Q140_L0.7040.37
8_C38_E0.7040.37
102_L118_L0.7030.37
16_P64_I0.6990.36
72_G131_D0.6990.36
6_P51_G0.6920.36
73_V113_P0.6900.36
124_F129_A0.6850.35
120_T167_P0.6810.35
168_P171_G0.6780.35
116_I171_G0.6780.35
87_V104_L0.6770.35
61_A137_S0.6760.35
44_E55_L0.6760.35
163_K182_L0.6730.35
65_Y69_L0.6660.34
57_A61_A0.6610.34
174_S183_F0.6570.33
92_I117_K0.6550.33
5_L11_E0.6540.33
148_Q156_N0.6540.33
33_I121_V0.6450.32
102_L131_D0.6400.32
61_A140_L0.6380.32
18_P155_L0.6370.32
120_T128_E0.6370.32
129_A152_R0.6340.32
16_P106_S0.6330.32
30_L148_Q0.6320.32
24_N89_L0.6300.31
103_V115_V0.6290.31
39_L129_A0.6260.31
72_G75_H0.6230.31
25_N60_D0.6220.31
48_L167_P0.6210.31
8_C122_Q0.6200.31
40_R47_L0.6190.31
131_D175_L0.6170.30
54_H120_T0.6150.30
39_L54_H0.6120.30
130_L174_S0.6110.30
37_W42_P0.6110.30
77_Q82_E0.6110.30
12_L47_L0.6100.30
41_L152_R0.6090.30
171_G175_L0.6040.30
159_V162_A0.6030.30
95_D117_K0.6020.29
50_G125_D0.6000.29
18_P21_W0.5990.29
90_I94_A0.5930.29
58_L126_L0.5910.29
53_E104_L0.5890.29
48_L72_G0.5870.28
12_L165_A0.5860.28
103_V119_S0.5860.28
25_N33_I0.5790.28
91_P169_W0.5790.28
76_P95_D0.5760.28
50_G123_L0.5750.28
22_A53_E0.5710.27
10_L100_H0.5700.27
79_S91_P0.5590.27
77_Q81_S0.5590.27
170_I175_L0.5570.26
22_A131_D0.5550.26
74_P161_L0.5530.26
137_S186_P0.5510.26
3_Y58_L0.5490.26
9_T155_L0.5490.26
12_L141_P0.5490.26
178_A183_F0.5440.26
75_H78_E0.5420.26
14_G31_I0.5410.25
82_E90_I0.5380.25
151_S156_N0.5380.25
15_L120_T0.5330.25
46_P133_L0.5310.25
101_R184_L0.5280.25
82_E162_A0.5260.25
119_S127_V0.5250.24
57_A87_V0.5240.24
24_N152_R0.5230.24
147_L151_S0.5230.24
17_D37_W0.5220.24
115_V180_V0.5210.24
81_S146_P0.5210.24
78_E83_S0.5210.24
67_Q126_L0.5190.24
4_T7_S0.5190.24
54_H128_E0.5160.24
95_D124_F0.5150.24
10_L14_G0.5150.24
81_S88_K0.5130.24
16_P31_I0.5120.24
79_S84_E0.5100.24
30_L141_P0.5090.24
69_L150_L0.5080.23
63_S176_A0.5070.23
26_E31_I0.5060.23
56_E110_N0.5030.23
94_A97_L0.5020.23
12_L133_L0.5000.23
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2qztA 2 0.2766 7.3 0.954 Contact Map
3bkrA 1 0.2926 5.8 0.956 Contact Map
1iktA 1 0.3085 5.5 0.957 Contact Map
2y69G 1 0.1809 4.4 0.959 Contact Map
2ww9B 1 0.2872 4.4 0.959 Contact Map
4w252 1 0.2872 4.1 0.959 Contact Map
3ivrA 2 0.4309 3.7 0.96 Contact Map
1pz4A 1 0.2979 3.6 0.96 Contact Map
1c44A 1 0.3191 3.6 0.96 Contact Map
4pzpA 1 0.4362 3.3 0.961 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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