GREMLIN Database
DUF4331 - Domain of unknown function (DUF4331)
PFAM: PF14224 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 422 (420)
Sequences: 4672 (3126)
Seq/√Len: 152.5
META: 0.919

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
60_E76_Q4.1211.00
126_G129_L3.6961.00
274_D292_Y3.3361.00
382_E386_V3.1551.00
368_F382_E2.9981.00
169_D198_D2.7921.00
360_V365_L2.7461.00
238_R254_W2.6891.00
43_E203_F2.6681.00
265_V270_I2.6501.00
130_P153_Y2.6301.00
24_V214_I2.5871.00
58_L254_W2.5721.00
170_D200_L2.5521.00
224_A227_P2.4921.00
294_T319_V2.4781.00
84_R105_R2.4651.00
20_V231_V2.4531.00
66_N72_D2.4501.00
17_N233_A2.4221.00
274_D277_R2.3801.00
58_L76_Q2.3671.00
73_I112_R2.3461.00
356_S362_G2.3201.00
92_N140_S2.2941.00
222_A227_P2.2261.00
62_H234_T2.2261.00
35_I207_S2.2251.00
145_A421_T2.2241.00
60_E236_S2.2221.00
28_R31_T2.2041.00
298_L386_V2.1951.00
42_E206_H2.1391.00
38_Y233_A2.1231.00
171_P412_F2.0991.00
35_I209_A2.0711.00
76_Q113_V2.0401.00
295_N300_A2.0231.00
38_Y235_A2.0071.00
239_Q253_P1.9761.00
110_V208_I1.9751.00
332_P335_V1.9651.00
183_L302_L1.9631.00
22_A231_V1.9501.00
290_A294_T1.9211.00
143_N146_A1.9201.00
37_N207_S1.8991.00
64_D68_D1.8711.00
55_D81_T1.8611.00
15_A57_V1.8501.00
62_H74_T1.8431.00
265_V293_V1.8371.00
277_R288_Q1.8231.00
161_G216_C1.8101.00
227_P346_A1.7871.00
129_L153_Y1.7821.00
145_A149_A1.7781.00
329_L333_T1.7421.00
179_I268_V1.7111.00
232_W340_M1.7101.00
62_H71_E1.7031.00
214_I345_T1.7031.00
154_T162_R1.6711.00
82_T105_R1.6691.00
18_T37_N1.6661.00
330_N333_T1.6631.00
27_D33_T1.6551.00
285_D288_Q1.6471.00
299_A386_V1.6461.00
327_P331_Q1.6401.00
17_N38_Y1.6321.00
162_R211_E1.6231.00
20_V233_A1.6141.00
169_D407_N1.6101.00
132_P151_A1.6031.00
329_L332_P1.5821.00
83_L272_L1.5781.00
25_S33_T1.5721.00
320_A327_P1.5621.00
95_T101_N1.5531.00
68_D72_D1.5491.00
112_R216_C1.5441.00
31_T211_E1.5411.00
277_R281_S1.5381.00
76_Q111_T1.4971.00
74_T113_V1.4751.00
9_I15_A1.4681.00
261_G341_L1.4621.00
32_V343_L1.4621.00
82_T107_T1.4541.00
270_I292_Y1.4511.00
32_V214_I1.4371.00
281_S288_Q1.4291.00
52_E104_V1.4251.00
237_R243_L1.4231.00
393_D396_P1.4221.00
148_A168_R1.4181.00
281_S289_F1.4121.00
349_P366_A1.4071.00
80_K107_T1.4041.00
164_F168_R1.4021.00
95_T99_D1.4011.00
351_P354_N1.3831.00
171_P419_L1.3821.00
53_F165_A1.3791.00
75_Y112_R1.3781.00
413_L420_A1.3771.00
298_L382_E1.3711.00
270_I293_V1.3661.00
128_N132_P1.3631.00
207_S419_L1.3551.00
58_L113_V1.3541.00
271_P274_D1.3541.00
185_G189_G1.3371.00
39_I206_H1.3361.00
410_V420_A1.3261.00
187_P190_P1.3211.00
281_S285_D1.3181.00
28_R162_R1.3181.00
265_V269_V1.3171.00
36_A233_A1.3151.00
83_L104_V1.3111.00
331_Q335_V1.3011.00
33_T211_E1.2981.00
20_V34_L1.2951.00
190_P193_A1.2901.00
14_K54_D1.2831.00
334_G337_P1.2831.00
395_A398_L1.2771.00
60_E74_T1.2761.00
88_T297_E1.2751.00
34_L231_V1.2601.00
327_P332_P1.2561.00
141_T147_L1.2551.00
81_T104_V1.2551.00
300_A304_A1.2541.00
139_R184_N1.2541.00
401_G405_D1.2501.00
58_L78_R1.2481.00
75_Y216_C1.2461.00
58_L236_S1.2461.00
71_E234_T1.2441.00
63_I217_L1.2381.00
155_L160_G1.2361.00
24_V349_P1.2361.00
114_R117_G1.2331.00
77_F110_V1.2291.00
350_T353_P1.2231.00
78_R111_T1.2221.00
322_F339_D1.2101.00
241_R251_S1.2071.00
34_L212_V1.2061.00
232_W282_E1.2051.00
154_T158_S1.2031.00
77_F163_V1.2011.00
311_P391_L1.2011.00
168_R413_L1.2001.00
294_T316_T1.1981.00
62_H236_S1.1951.00
149_A152_I1.1931.00
39_I53_F1.1881.00
277_R289_F1.1791.00
146_A150_Q1.1761.00
111_T124_V1.1761.00
217_L345_T1.1701.00
171_P374_L1.1671.00
39_I42_E1.1651.00
393_D398_L1.1551.00
394_P398_L1.1521.00
37_N171_P1.1511.00
110_V163_V1.1491.00
96_S99_D1.1451.00
353_P356_S1.1441.00
103_N272_L1.1431.00
218_T345_T1.1421.00
375_G412_F1.1411.00
183_L298_L1.1291.00
154_T161_G1.1281.00
41_L176_L1.1261.00
198_D407_N1.1190.99
282_E285_D1.1180.99
358_L380_D1.1160.99
245_G248_T1.1140.99
169_D420_A1.1130.99
374_L412_F1.1130.99
188_L191_N1.1120.99
11_K14_K1.1110.99
127_T132_P1.1060.99
380_D400_L1.0990.99
134_S167_Q1.0940.99
223_S227_P1.0930.99
70_K284_K1.0920.99
34_L63_I1.0890.99
186_A189_G1.0880.99
96_S101_N1.0870.99
86_P273_G1.0840.99
321_I385_A1.0830.99
185_G188_L1.0820.99
129_L132_P1.0820.99
186_A190_P1.0800.99
411_P420_A1.0780.99
212_V217_L1.0770.99
170_D198_D1.0730.99
328_G333_T1.0590.99
222_A226_N1.0580.99
394_P397_A1.0550.99
361_L389_A1.0540.99
307_G311_P1.0530.99
136_I141_T1.0460.99
401_G404_V1.0440.99
34_L217_L1.0390.99
36_A61_I1.0350.99
97_L150_Q1.0350.99
395_A399_P1.0330.99
290_A319_V1.0130.99
54_D57_V1.0120.99
316_T319_V1.0110.99
86_P89_F1.0100.99
217_L229_I1.0090.99
294_T314_P1.0060.99
278_F293_V1.0050.99
374_L419_L1.0050.99
309_P312_T1.0050.99
27_D211_E1.0010.99
8_L11_K0.9950.99
28_R211_E0.9910.99
244_G248_T0.9910.99
10_S14_K0.9900.99
321_I390_L0.9880.99
125_L128_N0.9840.99
239_Q255_V0.9840.99
290_A337_P0.9820.99
109_T124_V0.9820.99
97_L151_A0.9780.99
1_S260_L0.9700.99
104_V272_L0.9700.99
15_A235_A0.9690.99
75_Y161_G0.9640.98
393_D397_A0.9630.98
350_T354_N0.9620.98
33_T418_Y0.9610.98
131_V134_S0.9600.98
93_T103_N0.9560.98
325_G329_L0.9550.98
380_D402_D0.9540.98
37_N168_R0.9530.98
300_A303_N0.9490.98
78_R254_W0.9490.98
391_L397_A0.9390.98
41_L46_G0.9370.98
290_A323_L0.9330.98
326_I329_L0.9330.98
360_V363_G0.9320.98
303_N309_P0.9320.98
113_V118_R0.9300.98
357_R405_D0.9280.98
347_I366_A0.9220.98
327_P330_N0.9190.98
303_N310_A0.9170.98
325_G328_G0.9150.98
291_K295_N0.9140.98
246_D249_E0.9130.98
63_I75_Y0.9110.98
191_N194_G0.9100.98
30_D214_I0.9080.98
76_Q118_R0.9080.98
396_P399_P0.9050.98
27_D31_T0.9050.98
24_V30_D0.9040.98
17_N20_V0.9040.98
262_N382_E0.9040.98
407_N412_F0.9040.98
307_G310_A0.9040.98
25_S28_R0.9010.98
79_F208_I0.9010.98
407_N410_V0.8990.98
20_V260_L0.8980.98
11_K54_D0.8980.98
107_T130_P0.8980.98
84_R98_D0.8960.98
395_A400_L0.8950.98
176_L179_I0.8870.97
387_A398_L0.8870.97
12_D237_R0.8810.97
355_Q375_G0.8800.97
307_G312_T0.8790.97
165_A208_I0.8790.97
408_D411_P0.8780.97
163_V208_I0.8770.97
157_G161_G0.8760.97
396_P400_L0.8760.97
332_P337_P0.8750.97
60_E113_V0.8720.97
328_G331_Q0.8710.97
174_V379_V0.8700.97
295_N313_T0.8700.97
392_P397_A0.8680.97
179_I183_L0.8660.97
93_T101_N0.8640.97
360_V390_L0.8640.97
161_G212_V0.8620.97
212_V216_C0.8550.97
156_P159_T0.8530.97
80_K128_N0.8530.97
12_D15_A0.8520.97
308_L312_T0.8500.97
269_V386_V0.8470.97
135_N138_P0.8450.97
131_V167_Q0.8430.96
112_R161_G0.8330.96
5_E16_D0.8320.96
407_N420_A0.8300.96
301_L304_A0.8300.96
158_S161_G0.8280.96
352_A414_S0.8270.96
277_R280_A0.8260.96
377_D380_D0.8240.96
109_T127_T0.8210.96
394_P400_L0.8190.96
300_A312_T0.8170.96
186_A191_N0.8140.96
229_I345_T0.8140.96
326_I360_V0.8140.96
287_A290_A0.8110.96
139_R189_G0.8090.96
18_T39_I0.8090.96
187_P191_N0.8070.96
52_E55_D0.8050.95
185_G193_A0.8030.95
320_A324_T0.8020.95
69_G72_D0.8020.95
198_D201_A0.8010.95
69_G340_M0.7990.95
25_S31_T0.7980.95
85_N273_G0.7980.95
389_A392_P0.7970.95
299_A302_L0.7970.95
291_K294_T0.7950.95
65_N218_T0.7930.95
350_T356_S0.7930.95
293_V322_F0.7860.95
313_T391_L0.7860.95
327_P333_T0.7830.95
321_I326_I0.7820.95
300_A310_A0.7800.95
73_I212_V0.7800.95
148_A152_I0.7770.94
332_P336_T0.7770.94
185_G190_P0.7770.94
188_L193_A0.7760.94
402_D405_D0.7730.94
189_G193_A0.7720.94
268_V382_E0.7690.94
331_Q337_P0.7680.94
323_L338_A0.7670.94
153_Y163_V0.7660.94
310_A313_T0.7630.94
394_P399_P0.7610.94
18_T205_V0.7610.94
45_A199_T0.7610.94
78_R109_T0.7590.94
126_G132_P0.7590.94
145_A148_A0.7590.94
302_L387_A0.7560.94
383_L400_L0.7550.94
384_R397_A0.7540.94
31_T159_T0.7520.93
173_F374_L0.7520.93
168_R419_L0.7510.93
407_N411_P0.7500.93
96_S150_Q0.7490.93
308_L311_P0.7490.93
368_F381_I0.7490.93
8_L12_D0.7480.93
71_E74_T0.7470.93
389_A397_A0.7460.93
17_N258_S0.7430.93
188_L192_A0.7420.93
146_A149_A0.7420.93
64_D69_G0.7420.93
168_R207_S0.7410.93
295_N314_P0.7400.93
261_G340_M0.7370.93
111_T122_R0.7360.93
219_R226_N0.7350.93
412_F420_A0.7350.93
229_I343_L0.7340.93
389_A398_L0.7300.92
14_K39_I0.7300.92
185_G192_A0.7300.92
187_P192_A0.7290.92
238_R251_S0.7270.92
34_L61_I0.7260.92
124_V128_N0.7240.92
15_A237_R0.7200.92
64_D67_G0.7150.92
413_L421_T0.7140.92
387_A390_L0.7120.92
128_N133_P0.7110.91
91_Y136_I0.7110.91
84_R99_D0.7080.91
243_L249_E0.7070.91
41_L200_L0.7050.91
71_E284_K0.7050.91
71_E282_E0.7040.91
211_E418_Y0.7040.91
128_N131_V0.7040.91
97_L147_L0.7040.91
171_P200_L0.7020.91
115_G118_R0.7020.91
214_I217_L0.7010.91
331_Q334_G0.6990.91
43_E202_G0.6990.91
24_V27_D0.6980.91
265_V322_F0.6960.91
361_L365_L0.6960.91
126_G130_P0.6950.91
9_I17_N0.6920.90
271_P297_E0.6900.90
53_F79_F0.6900.90
265_V339_D0.6890.90
380_D401_G0.6880.90
114_R118_R0.6860.90
358_L361_L0.6860.90
245_G250_V0.6860.90
129_L134_S0.6850.90
32_V229_I0.6840.90
328_G332_P0.6820.90
110_V165_A0.6820.90
2_S5_E0.6810.90
148_A413_L0.6810.90
268_V379_V0.6810.90
274_D291_K0.6790.89
58_L238_R0.6780.89
262_N368_F0.6780.89
406_A411_P0.6770.89
169_D202_G0.6740.89
262_N322_F0.6740.89
95_T100_P0.6710.89
298_L301_L0.6690.89
108_Y163_V0.6680.89
293_V323_L0.6670.89
167_Q206_H0.6660.89
161_G210_L0.6640.88
97_L100_P0.6630.88
85_N297_E0.6620.88
303_N308_L0.6600.88
391_L396_P0.6580.88
243_L255_V0.6580.88
348_P366_A0.6570.88
357_R361_L0.6560.88
155_L161_G0.6560.88
184_N306_Y0.6560.88
32_V217_L0.6540.88
244_G250_V0.6540.88
330_N335_V0.6530.88
63_I229_I0.6530.88
132_P167_Q0.6530.88
4_R13_P0.6520.88
61_I77_F0.6520.88
226_N332_P0.6510.87
21_Y37_N0.6500.87
358_L362_G0.6480.87
318_L386_V0.6460.87
163_V210_L0.6450.87
400_L403_G0.6440.87
17_N279_N0.6440.87
286_D290_A0.6420.87
71_E232_W0.6400.87
239_Q242_V0.6370.86
135_N141_T0.6350.86
147_L151_A0.6340.86
218_T227_P0.6320.86
102_L141_T0.6320.86
335_V338_A0.6310.86
51_Y167_Q0.6300.86
350_T366_A0.6280.86
173_F404_V0.6280.86
262_N341_L0.6250.85
23_F26_P0.6240.85
132_P141_T0.6240.85
129_L133_P0.6240.85
308_L313_T0.6230.85
320_A326_I0.6210.85
387_A400_L0.6210.85
60_E71_E0.6210.85
35_I419_L0.6210.85
362_G365_L0.6200.85
309_P313_T0.6150.84
35_I170_D0.6140.84
244_G247_G0.6140.84
287_A337_P0.6130.84
25_S211_E0.6120.84
333_T337_P0.6090.84
322_F386_V0.6090.84
240_V250_V0.6060.83
300_A313_T0.6050.83
384_R389_A0.6040.83
189_G192_A0.6030.83
17_N36_A0.6030.83
389_A396_P0.6020.83
319_V323_L0.6020.83
391_L394_P0.6010.83
385_A390_L0.6000.83
397_A401_G0.5980.83
299_A304_A0.5980.83
64_D70_K0.5970.83
110_V126_G0.5940.82
381_I385_A0.5930.82
396_P401_G0.5910.82
125_L132_P0.5900.82
75_Y212_V0.5900.82
303_N312_T0.5890.82
178_A184_N0.5890.82
186_A193_A0.5890.82
95_T98_D0.5880.82
52_E81_T0.5870.81
243_L251_S0.5840.81
186_A192_A0.5840.81
222_A225_T0.5840.81
173_F179_I0.5830.81
187_P193_A0.5820.81
247_G250_V0.5820.81
223_S226_N0.5810.81
124_V127_T0.5800.81
52_E206_H0.5800.81
47_G180_F0.5740.80
110_V210_L0.5740.80
18_T170_D0.5730.80
150_Q153_Y0.5730.80
42_E167_Q0.5730.80
22_A343_L0.5730.80
260_L267_E0.5720.80
119_T123_T0.5720.80
322_F382_E0.5700.80
168_R171_P0.5690.79
14_K57_V0.5690.79
310_A314_P0.5680.79
393_D400_L0.5660.79
126_G161_G0.5660.79
410_V421_T0.5650.79
297_E300_A0.5640.79
39_I52_E0.5640.79
65_N73_I0.5620.79
90_L297_E0.5620.79
77_F208_I0.5610.79
350_T355_Q0.5560.78
97_L105_R0.5560.78
80_K109_T0.5560.78
408_D412_F0.5540.78
380_D384_R0.5530.78
86_P90_L0.5530.78
179_I379_V0.5530.78
41_L203_F0.5530.78
185_G191_N0.5520.77
218_T346_A0.5510.77
243_L252_G0.5510.77
263_P368_F0.5500.77
52_E56_D0.5500.77
274_D289_F0.5500.77
373_R376_D0.5460.77
384_R401_G0.5450.77
331_Q336_T0.5440.76
116_G121_T0.5440.76
220_D345_T0.5440.76
176_L200_L0.5430.76
70_K116_G0.5430.76
380_D389_A0.5410.76
360_V364_D0.5410.76
83_L105_R0.5410.76
35_I374_L0.5410.76
400_L405_D0.5400.76
96_S100_P0.5370.75
214_I218_T0.5370.75
236_S256_Q0.5370.75
184_N191_N0.5330.75
123_T126_G0.5330.75
87_N292_Y0.5330.75
71_E115_G0.5310.75
298_L302_L0.5310.75
361_L381_I0.5300.75
94_G101_N0.5300.75
301_L305_L0.5300.75
45_A203_F0.5300.75
36_A235_A0.5290.74
102_L132_P0.5290.74
417_P422_P0.5290.74
134_S206_H0.5280.74
399_P402_D0.5280.74
98_D105_R0.5270.74
85_N103_N0.5270.74
171_P209_A0.5260.74
173_F375_G0.5260.74
116_G119_T0.5260.74
57_V235_A0.5260.74
22_A341_L0.5250.74
126_G131_V0.5250.74
125_L129_L0.5240.74
11_K52_E0.5240.74
69_G338_A0.5230.74
45_A202_G0.5220.73
61_I231_V0.5210.73
329_L337_P0.5210.73
5_E10_S0.5210.73
85_N93_T0.5200.73
98_D107_T0.5200.73
323_L337_P0.5190.73
248_T251_S0.5180.73
175_D178_A0.5180.73
3_H7_P0.5180.73
341_L368_F0.5160.73
22_A34_L0.5130.72
13_P42_E0.5130.72
65_N68_D0.5120.72
324_T328_G0.5120.72
391_L398_L0.5110.72
408_D420_A0.5110.72
168_R421_T0.5100.72
114_R119_T0.5090.72
174_V200_L0.5080.71
126_G163_V0.5080.71
329_L334_G0.5070.71
297_E301_L0.5060.71
3_H6_A0.5050.71
26_P31_T0.5040.71
75_Y210_L0.5010.70
270_I274_D0.5010.70
326_I391_L0.5010.70
83_L103_N0.5000.70
179_I199_T0.5000.70
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2xn2A 4 0.2133 2.2 0.985 Contact Map
3af5A 1 0.1422 1.9 0.985 Contact Map
2ycbA 2 0.1422 1.8 0.985 Contact Map
2xr1A 2 0.1422 1.8 0.985 Contact Map
2yfoA 4 0.2275 1.5 0.986 Contact Map
3tguG 1 0.1185 1.5 0.986 Contact Map
1pp9G 1 0.1185 1.5 0.986 Contact Map
2znlB 1 0.0355 1.3 0.986 Contact Map
1fm0E 2 0.1185 1.1 0.987 Contact Map
3eujB 2 0.1185 1.1 0.987 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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