GREMLIN Database
DUF4328 - Domain of unknown function (DUF4328)
PFAM: PF14219 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 167 (156)
Sequences: 4650 (4043)
Seq/√Len: 323.7
META: 0.825

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
59_G63_G3.7581.00
70_A74_R3.6011.00
39_L79_M3.4061.00
76_Y80_R3.2151.00
35_F158_A3.1901.00
69_I73_W2.9671.00
64_G74_R2.9531.00
64_G70_A2.8511.00
106_G110_A2.7411.00
36_L62_V2.4391.00
74_R77_Q2.4121.00
55_R81_E2.2601.00
76_Y106_G2.1731.00
39_L65_W2.1211.00
156_V160_L2.0971.00
111_L157_L1.8881.00
42_A82_I1.8491.00
63_G66_F1.8121.00
37_V41_R1.7791.00
40_Y44_R1.7651.00
35_F39_L1.7201.00
32_A36_L1.7141.00
56_F77_Q1.6741.00
22_L26_L1.6721.00
60_W63_G1.6441.00
56_F74_R1.6111.00
120_R124_R1.5791.00
158_A161_V1.5491.00
113_L117_V1.5301.00
46_A85_A1.5081.00
60_W67_V1.4841.00
115_S151_S1.4651.00
13_A16_L1.4151.00
111_L153_A1.4121.00
73_W77_Q1.3891.00
107_L110_A1.3821.00
43_R47_R1.3681.00
160_L164_R1.3651.00
133_T136_E1.3561.00
28_L32_A1.3281.00
42_A165_I1.3081.00
103_A107_L1.3051.00
43_R46_A1.2891.00
33_V37_V1.2871.00
44_R58_P1.2851.00
102_P105_L1.2661.00
13_A17_V1.2511.00
39_L78_V1.2501.00
43_R54_L1.2471.00
41_R45_N1.2361.00
11_D15_A1.2331.00
46_A54_L1.2311.00
67_V70_A1.2291.00
39_L82_I1.2281.00
16_L20_A1.2071.00
40_Y62_V1.1721.00
79_M161_V1.1531.00
36_L65_W1.1471.00
30_A34_L1.1381.00
159_I163_R1.1361.00
43_R58_P1.1321.00
54_L85_A1.1311.00
122_A140_A1.1261.00
36_L39_L1.1251.00
45_N49_L1.1101.00
80_R84_R1.1021.00
65_W78_V1.0991.00
110_A114_A1.0971.00
47_R54_L1.0841.00
12_A16_L1.0801.00
138_A142_V1.0681.00
39_L43_R1.0421.00
104_L160_L1.0381.00
56_F78_V1.0241.00
107_L157_L1.0201.00
45_N48_A1.0131.00
84_R102_P1.0131.00
64_G78_V1.0111.00
138_A141_T1.0031.00
10_A14_D1.0021.00
15_A18_G0.9941.00
25_L29_L0.9881.00
65_W79_M0.9771.00
142_V146_V0.9751.00
75_P109_W0.9651.00
82_I165_I0.9591.00
155_A158_A0.9571.00
76_Y105_L0.9521.00
118_L150_L0.9521.00
157_L161_V0.9391.00
154_A157_L0.9361.00
40_Y58_P0.9271.00
43_R82_I0.9261.00
158_A162_V0.9241.00
46_A51_G0.9151.00
44_R48_A0.9091.00
43_R78_V0.9081.00
50_G53_G0.9041.00
39_L61_A0.8961.00
135_D138_A0.8731.00
43_R61_A0.8651.00
107_L111_L0.8651.00
111_L115_S0.8611.00
43_R81_E0.8551.00
104_L161_V0.8471.00
31_T35_F0.8451.00
93_G96_W0.8381.00
67_V74_R0.8361.00
46_A82_I0.8301.00
104_L164_R0.8301.00
60_W64_G0.8251.00
27_L31_T0.8241.00
85_A96_W0.8171.00
36_L66_F0.8171.00
56_F81_E0.8161.00
54_L81_E0.8131.00
45_N86_S0.8061.00
122_A144_S0.8021.00
132_D136_E0.8001.00
112_W116_N0.7981.00
21_G24_Q0.7861.00
47_R58_P0.7821.00
43_R64_G0.7711.00
13_A20_A0.7691.00
144_S148_D0.7641.00
18_G22_L0.7641.00
61_A78_V0.7641.00
38_W162_V0.7620.99
31_T34_L0.7600.99
117_V121_I0.7590.99
46_A81_E0.7540.99
24_Q148_D0.7540.99
149_L153_A0.7470.99
79_M108_W0.7460.99
130_S133_T0.7440.99
118_L147_S0.7420.99
35_F65_W0.7350.99
154_A158_A0.7330.99
32_A155_A0.7260.99
47_R50_G0.7250.99
70_A73_W0.7250.99
17_V20_A0.7180.99
51_G54_L0.7040.99
58_P61_A0.7020.99
32_A35_F0.7000.99
83_W165_I0.6970.99
115_S150_L0.6960.99
105_L161_V0.6950.99
71_N116_N0.6900.99
111_L154_A0.6880.99
83_W102_P0.6860.99
34_L37_V0.6840.99
117_V120_R0.6800.99
49_L86_S0.6720.99
44_R47_R0.6720.99
74_R78_V0.6710.99
83_W86_S0.6710.99
56_F64_G0.6680.99
81_E85_A0.6680.99
14_D17_V0.6640.99
119_G147_S0.6630.99
161_V165_I0.6630.99
57_S63_G0.6620.99
64_G67_V0.6600.99
80_R105_L0.6580.99
15_A19_L0.6550.98
83_W164_R0.6460.98
56_F61_A0.6430.98
26_L30_A0.6430.98
101_V106_G0.6430.98
58_P62_V0.6390.98
46_A50_G0.6370.98
87_D99_R0.6360.98
42_A45_N0.6330.98
84_R96_W0.6320.98
18_G21_G0.6260.98
19_L23_L0.6200.98
92_A96_W0.6190.98
128_S132_D0.6160.98
95_D98_P0.6160.98
13_A141_T0.6150.98
131_A136_E0.6070.98
35_F162_V0.6030.97
135_D139_D0.6010.97
40_Y61_A0.6000.97
36_L64_G0.6000.97
65_W75_P0.6000.97
123_F129_D0.5950.97
127_L130_S0.5930.97
115_S154_A0.5920.97
50_G54_L0.5860.97
79_M82_I0.5830.97
116_N120_R0.5830.97
37_V62_V0.5750.97
122_A147_S0.5730.97
36_L40_Y0.5730.97
75_P108_W0.5710.96
12_A15_A0.5670.96
103_A106_G0.5650.96
153_A157_L0.5650.96
160_L163_R0.5600.96
140_A144_S0.5550.96
141_T144_S0.5520.96
112_W155_A0.5520.96
139_D143_L0.5470.95
61_A66_F0.5460.95
48_A88_P0.5450.95
64_G75_P0.5440.95
62_V66_F0.5430.95
102_P106_G0.5410.95
79_M105_L0.5410.95
60_W74_R0.5370.95
152_I155_A0.5300.95
67_V71_N0.5250.94
121_I125_L0.5240.94
53_G96_W0.5230.94
84_R101_V0.5210.94
23_L26_L0.5200.94
145_I148_D0.5200.94
72_L113_L0.5200.94
86_S166_T0.5190.94
112_W115_S0.5190.94
119_G124_R0.5190.94
72_L116_N0.5180.94
153_A156_V0.5160.94
96_W99_R0.5110.94
136_E139_D0.5100.93
152_I156_V0.5060.93
80_R106_G0.5050.93
162_V166_T0.5050.93
139_D142_V0.5030.93
146_V149_L0.5030.93
143_L146_V0.5020.93
25_L28_L0.5000.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2l2tA 2 0.2635 2.2 0.937 Contact Map
3lb6C 1 0.0599 2.2 0.937 Contact Map
4xk8I 1 0.1737 2.2 0.938 Contact Map
2k9pA 1 0.2635 2 0.939 Contact Map
2mfrA 1 0.3413 1.8 0.941 Contact Map
1jb0I 1 0.2275 1.6 0.942 Contact Map
2ks1B 1 0.2635 1.6 0.942 Contact Map
3wdoA 1 0.4371 1.3 0.945 Contact Map
2kogA 1 0.2575 1.3 0.946 Contact Map
2lx0A 1 0.1916 1.2 0.947 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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