GREMLIN Database
DUF4316 - Domain of unknown function (DUF4316)
PFAM: PF14195 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 77 (72)
Sequences: 646 (433)
Seq/√Len: 51.0
META: 0.913

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
10_M16_Y3.4181.00
16_Y19_I2.8651.00
6_K10_M2.5021.00
50_S55_L2.4901.00
2_E6_K2.2361.00
10_M19_I2.0371.00
3_N23_I2.0191.00
69_K72_S2.0181.00
7_N11_S1.8790.99
69_K73_Q1.8730.99
8_A19_I1.8640.99
8_A16_Y1.8530.99
60_E63_A1.6730.98
14_Q17_N1.5330.97
12_T19_I1.5010.97
48_K55_L1.4630.96
3_N21_G1.4570.96
12_T23_I1.4050.95
26_G29_E1.3920.95
70_E73_Q1.3180.93
40_E43_E1.3090.93
48_K51_V1.3050.93
61_E67_K1.2930.93
68_A71_K1.2420.91
7_N10_M1.2380.91
2_E18_M1.2140.90
68_A72_S1.2130.90
56_K59_K1.1760.89
51_V56_K1.1330.87
32_V39_A1.0860.84
12_T15_N1.0680.83
3_N6_K1.0520.82
4_P23_I1.0380.82
58_K62_A1.0280.81
33_A36_E1.0180.80
45_P48_K1.0070.80
58_K61_E0.9940.79
31_T39_A0.9820.78
12_T18_M0.9780.77
34_E39_A0.9780.77
30_P38_K0.9530.76
30_P35_L0.9480.75
63_A66_K0.9450.75
6_K12_T0.9410.75
27_P30_P0.9130.72
68_A73_Q0.9100.72
7_N12_T0.8810.70
14_Q24_N0.8760.69
12_T16_Y0.8730.69
10_M14_Q0.8670.68
8_A11_S0.8430.66
49_P52_L0.8380.66
67_K70_E0.8340.65
10_M18_M0.8240.65
34_E37_A0.8050.63
2_E7_N0.8050.63
67_K73_Q0.7960.62
7_N21_G0.7940.62
62_A66_K0.7910.61
6_K15_N0.7870.61
56_K66_K0.7710.59
53_E57_A0.7570.58
31_T34_E0.7570.58
64_K68_A0.7520.57
31_T36_E0.7510.57
11_S22_I0.7220.54
15_N26_G0.7210.54
4_P8_A0.7180.54
61_E64_K0.7100.53
52_L58_K0.6950.52
46_E53_E0.6850.51
51_V58_K0.6770.50
36_E39_A0.6710.49
51_V55_L0.6670.49
15_N18_M0.6670.49
52_L55_L0.6610.48
34_E38_K0.6530.47
57_A70_E0.6500.47
2_E10_M0.6340.45
54_Q57_A0.6200.44
40_E46_E0.6170.44
39_A42_E0.6140.43
59_K63_A0.6110.43
6_K21_G0.6090.43
52_L59_K0.6080.43
28_K34_E0.5970.42
10_M15_N0.5940.41
2_E15_N0.5910.41
6_K16_Y0.5910.41
30_P39_A0.5900.41
7_N19_I0.5870.41
32_V38_K0.5700.39
53_E60_E0.5680.39
57_A64_K0.5670.39
63_A70_E0.5590.38
32_V37_A0.5510.37
56_K62_A0.5450.36
64_K70_E0.5330.35
4_P16_Y0.5330.35
35_L39_A0.5310.35
40_E47_K0.5300.35
35_L38_K0.5250.35
33_A38_K0.5230.34
62_A65_K0.5210.34
53_E73_Q0.5110.33
64_K67_K0.5070.33
14_Q28_K0.5060.33
43_E72_S0.5000.32
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3ja83 1 0 6.1 0.895 Contact Map
3t49A 1 0.3247 2.9 0.91 Contact Map
1ywfA 1 0.5325 2.7 0.912 Contact Map
2winM 1 0.3506 1.8 0.92 Contact Map
4cpcA 4 0.5974 1.3 0.927 Contact Map
2xkxA 2 0.7013 1.2 0.928 Contact Map
4yhuA 1 0 1.1 0.929 Contact Map
5a1uH 1 0.4675 1 0.931 Contact Map
1o7dB 1 0.2208 1 0.931 Contact Map
4fgvA 1 0.4286 1 0.931 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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