GREMLIN Database
DUF4294 - Domain of unknown function (DUF4294)
PFAM: PF14127 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 155 (152)
Sequences: 4867 (2912)
Seq/√Len: 236.2
META: 0.932

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
61_S64_E3.8221.00
34_N91_Q3.5771.00
112_K117_G3.0601.00
139_D142_G2.7971.00
138_Y150_E2.7671.00
81_E85_K2.5661.00
35_V94_I2.5461.00
34_N90_T2.5391.00
128_R133_N2.3481.00
32_V36_K2.1401.00
77_K81_E2.1321.00
55_R58_T2.0261.00
55_R68_Y2.0231.00
89_I132_S1.9631.00
21_N24_E1.9441.00
22_K25_R1.8511.00
25_R29_T1.8381.00
96_I132_S1.8031.00
48_R51_E1.7611.00
53_N57_A1.7591.00
59_L68_Y1.7511.00
44_L48_R1.6591.00
52_L72_V1.6491.00
48_R79_E1.5321.00
46_G102_E1.5251.00
51_E55_R1.5191.00
39_Y43_K1.5091.00
94_I149_I1.4971.00
118_F122_F1.4901.00
35_V152_I1.4881.00
127_A132_S1.4761.00
43_K46_G1.4701.00
62_K65_R1.4581.00
83_E87_L1.4401.00
97_K146_D1.4361.00
71_K75_E1.4361.00
125_T129_L1.4181.00
59_L64_E1.4131.00
23_K27_R1.4081.00
31_L94_I1.3951.00
115_R119_S1.3881.00
30_R34_N1.3831.00
121_F125_T1.3821.00
31_L149_I1.3641.00
56_L59_L1.3261.00
100_D137_E1.3221.00
33_R91_Q1.3031.00
64_E67_K1.3001.00
45_A99_I1.2911.00
97_K143_E1.2591.00
44_L79_E1.2301.00
52_L68_Y1.2261.00
52_L69_I1.2241.00
46_G50_N1.2141.00
111_I134_L1.2131.00
74_K78_K1.1961.00
54_E58_T1.1891.00
69_I115_R1.1831.00
17_L151_R1.1751.00
122_F126_V1.1701.00
54_E57_A1.1651.00
63_K67_K1.1551.00
48_R52_L1.1551.00
88_T91_Q1.1501.00
96_I134_L1.1411.00
68_Y72_V1.1341.00
62_K66_K1.1271.00
93_K132_S1.1221.00
105_N109_E1.1101.00
22_K26_R1.1101.00
99_I107_S1.0991.00
29_T32_V1.0991.00
89_I131_G1.0971.00
82_D85_K1.0841.00
119_S123_W1.0821.00
52_L55_R1.0711.00
35_V149_I1.0581.00
60_P64_E1.0401.00
19_F24_E1.0391.00
23_K26_R1.0251.00
125_T128_R1.0121.00
44_L47_E1.0051.00
72_V75_E1.0031.00
124_Q133_N0.9951.00
50_N54_E0.9931.00
18_K21_N0.9931.00
37_K91_Q0.9751.00
28_Y32_V0.9651.00
24_E27_R0.9641.00
148_L152_I0.9571.00
148_L151_R0.9441.00
81_E123_W0.9421.00
67_K71_K0.9391.00
105_N113_E0.9341.00
124_Q128_R0.9161.00
34_N88_T0.9031.00
100_D107_S0.9021.00
19_F25_R0.9011.00
70_K115_R0.8950.99
56_L69_I0.8930.99
27_R31_L0.8920.99
56_L114_L0.8870.99
111_I114_L0.8860.99
55_R71_K0.8830.99
41_Y44_L0.8830.99
38_V42_A0.8820.99
51_E68_Y0.8760.99
48_R72_V0.8740.99
77_K80_F0.8730.99
59_L62_K0.8730.99
117_G121_F0.8680.99
84_L87_L0.8640.99
75_E79_E0.8570.99
7_E10_E0.8400.99
49_L103_T0.8370.99
20_K24_E0.8360.99
55_R59_L0.8220.99
79_E83_E0.8150.99
133_N136_E0.8090.99
116_G119_S0.8080.99
38_V94_I0.8010.99
43_K102_E0.7940.99
73_E77_K0.7720.99
43_K47_E0.7690.98
30_R33_R0.7690.98
93_K136_E0.7650.98
12_Y15_P0.7650.98
41_Y80_F0.7630.98
34_N94_I0.7540.98
53_N105_N0.7480.98
49_L53_N0.7470.98
19_F28_Y0.7460.98
106_T137_E0.7400.98
119_S126_V0.7390.98
87_L92_G0.7350.98
33_R37_K0.7280.98
6_I9_P0.7240.98
57_A60_P0.7220.98
16_K19_F0.7200.98
76_L111_I0.7190.98
127_A134_L0.7110.97
52_L56_L0.7110.97
49_L110_L0.6960.97
101_R150_E0.6950.97
80_F83_E0.6940.97
75_E78_K0.6920.97
141_E147_R0.6890.97
78_K82_D0.6890.97
72_V80_F0.6870.97
45_A84_L0.6830.97
28_Y148_L0.6830.97
56_L65_R0.6780.97
147_R151_R0.6770.97
93_K96_I0.6740.97
50_N53_N0.6710.96
44_L80_F0.6670.96
41_Y83_E0.6620.96
107_S110_L0.6610.96
70_K74_K0.6600.96
89_I96_I0.6590.96
30_R90_T0.6590.96
42_A46_G0.6580.96
14_Y19_F0.6520.96
89_I127_A0.6490.96
19_F22_K0.6470.96
27_R30_R0.6440.95
38_V87_L0.6380.95
17_L21_N0.6260.95
108_Y121_F0.6230.95
126_V130_F0.6220.95
139_D143_E0.6180.94
7_E14_Y0.6160.94
100_D106_T0.6160.94
39_Y94_I0.6150.94
38_V88_T0.6130.94
49_L114_L0.6080.94
123_W132_S0.6040.94
53_N110_L0.5980.93
14_Y21_N0.5940.93
91_Q94_I0.5870.93
44_L83_E0.5860.93
73_E124_Q0.5820.92
109_E112_K0.5810.92
31_L152_I0.5810.92
111_I115_R0.5790.92
106_T109_E0.5770.92
149_I152_I0.5740.92
9_P111_I0.5740.92
45_A76_L0.5680.91
90_T94_I0.5660.91
143_E148_L0.5660.91
77_K115_R0.5600.91
42_A45_A0.5590.91
33_R88_T0.5570.91
42_A95_L0.5570.91
74_K77_K0.5540.90
136_E143_E0.5520.90
46_G103_T0.5490.90
3_I9_P0.5490.90
72_V114_L0.5460.90
124_Q134_L0.5430.90
123_W126_V0.5400.89
96_I107_S0.5390.89
69_I73_E0.5390.89
53_N113_E0.5380.89
9_P12_Y0.5380.89
14_Y154_Q0.5380.89
35_V98_L0.5370.89
30_R91_Q0.5360.89
34_N38_V0.5350.89
34_N37_K0.5350.89
124_Q135_K0.5350.89
5_V18_K0.5330.89
37_K83_E0.5310.89
26_R29_T0.5270.88
60_P63_K0.5260.88
23_K29_T0.5220.88
90_T93_K0.5210.88
138_Y143_E0.5180.87
53_N114_L0.5160.87
77_K123_W0.5150.87
6_I14_Y0.5140.87
47_E51_E0.5130.87
28_Y152_I0.5110.87
73_E130_F0.5090.86
92_G95_L0.5040.86
114_L117_G0.5020.86
77_K111_I0.5000.85
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1pxyA 1 0.6 14.4 0.947 Contact Map
1h67A 1 0.6 13.1 0.948 Contact Map
4x86A 1 0.1935 12.2 0.949 Contact Map
1wynA 1 0.6903 10.8 0.95 Contact Map
1wypA 1 0.6903 9.4 0.952 Contact Map
4yv9A 3 0.4903 9.3 0.952 Contact Map
2wujA 2 0.2129 8.1 0.953 Contact Map
1sz9A 1 0.6387 7.6 0.953 Contact Map
2q5xA 1 0.5613 6.9 0.954 Contact Map
1dxxA 2 0.6129 6.8 0.955 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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