GREMLIN Database
DUF4274 - Domain of unknown function (DUF4274)
PFAM: PF14096 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 76 (74)
Sequences: 899 (759)
Seq/√Len: 88.2
META: 0.834

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
40_F72_E3.8931.00
7_H35_T3.4121.00
27_E70_E2.9101.00
15_W43_A2.8191.00
13_Y18_G2.5581.00
6_L28_N2.3111.00
33_L75_I2.3011.00
10_A39_I2.2811.00
38_L42_L2.1721.00
31_C36_A2.0421.00
54_R66_D2.0331.00
6_L24_W1.9981.00
24_W28_N1.9511.00
26_I39_I1.7491.00
23_R70_E1.6221.00
6_L31_C1.5421.00
11_L38_L1.5211.00
13_Y17_D1.5211.00
23_R27_E1.4951.00
21_V25_I1.4600.99
7_H38_L1.3250.99
41_W45_P1.2910.99
67_L71_I1.2780.99
70_E74_R1.2730.98
22_L39_I1.2340.98
66_D69_K1.2160.98
40_F49_L1.2060.98
3_P7_H1.1630.97
43_A68_L1.1610.97
9_F21_V1.1510.97
8_L12_N1.1330.97
47_Y50_Q1.1120.96
6_L30_N1.0920.96
13_Y21_V1.0720.96
8_L11_L1.0270.94
25_I31_C0.9630.92
41_W72_E0.9620.92
19_F67_L0.9410.91
56_E59_D0.8910.89
55_E58_P0.8890.89
2_S5_E0.8630.87
44_D47_Y0.8470.86
21_V43_A0.8260.85
51_Y58_P0.8260.85
48_Y68_L0.8250.85
65_F69_K0.8030.83
5_E9_F0.7930.82
26_I67_L0.7770.81
10_A35_T0.7760.81
54_R57_V0.7670.80
5_E41_W0.7660.80
19_F64_W0.7660.80
62_R73_E0.7640.80
52_A56_E0.7520.79
11_L42_L0.7510.79
40_F47_Y0.7300.77
20_E55_E0.7230.76
25_I28_N0.7200.76
17_D21_V0.7200.76
43_A67_L0.7110.75
9_F25_I0.7080.75
14_N18_G0.6970.74
2_S8_L0.6920.73
64_W68_L0.6800.72
21_V24_W0.6790.72
51_Y55_E0.6680.70
63_E66_D0.6550.69
60_Y63_E0.6540.69
32_D35_T0.6530.69
55_E59_D0.6460.68
48_Y64_W0.6310.66
26_I70_E0.6260.65
57_V62_R0.6200.65
34_G37_L0.6190.65
49_L74_R0.6110.64
48_Y61_E0.6010.62
55_E60_Y0.5950.62
54_R58_P0.5900.61
13_Y34_G0.5860.60
9_F42_L0.5850.60
15_W19_F0.5810.60
21_V40_F0.5780.59
14_N19_F0.5710.58
19_F45_P0.5700.58
17_D20_E0.5680.58
35_T38_L0.5660.58
29_P67_L0.5650.58
16_D47_Y0.5630.57
15_W39_I0.5600.57
5_E29_P0.5590.57
15_W18_G0.5540.56
68_L74_R0.5500.56
9_F12_N0.5490.56
58_P62_R0.5480.55
15_W42_L0.5440.55
69_K73_E0.5440.55
3_P6_L0.5430.55
19_F63_E0.5350.54
36_A39_I0.5320.53
3_P73_E0.5260.52
22_L43_A0.5260.52
6_L25_I0.5230.52
10_A22_L0.5210.52
36_A74_R0.5200.52
4_E8_L0.5120.51
63_E68_L0.5070.50
59_D62_R0.5060.50
20_E56_E0.5040.49
37_L75_I0.5040.49
15_W67_L0.5010.49
50_Q73_E0.5000.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3waiA 1 0.8684 8.6 0.896 Contact Map
3ru6A 2 0 4.4 0.909 Contact Map
2lgzA 1 0.8158 3.1 0.916 Contact Map
2d1gA 2 0.9079 3 0.916 Contact Map
2kr1A 1 0.1842 3 0.916 Contact Map
2itbA 2 0.8947 2.9 0.917 Contact Map
2uv8G 3 1 2.6 0.919 Contact Map
2qtlA 1 1 2.4 0.92 Contact Map
1rjdA 1 0.3421 2.4 0.921 Contact Map
1f5qB 1 0.3947 2.3 0.921 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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