GREMLIN Database
DUF4270 - Domain of unknown function (DUF4270)
PFAM: PF14092 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 446 (424)
Sequences: 4674 (4259)
Seq/√Len: 206.8
META: 0.85

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
106_E173_K7.6981.00
164_P167_D4.3171.00
278_L288_T4.0501.00
172_Q176_D3.5791.00
80_D221_Y3.2491.00
58_F199_I3.1861.00
83_V162_R3.1611.00
136_G140_V3.1291.00
103_S200_K3.1051.00
103_S202_D3.0561.00
186_N189_N2.9861.00
27_T286_V2.9161.00
79_I220_I2.8441.00
26_Y289_K2.7191.00
82_V170_G2.7121.00
420_T423_Y2.7111.00
76_N225_D2.6351.00
142_D146_N2.5441.00
105_Y200_K2.5201.00
82_V220_I2.4811.00
312_E379_T2.3501.00
136_G139_T2.3051.00
177_K182_E2.3051.00
101_K202_D2.2971.00
25_A278_L2.2651.00
83_V164_P2.2551.00
64_L187_N2.2451.00
405_G408_L2.2341.00
85_V160_Q2.2201.00
45_S55_T2.2181.00
82_V218_L2.2071.00
57_S254_H2.1961.00
314_V377_Y2.1911.00
419_R422_L2.1381.00
85_V219_T2.1311.00
312_E441_E2.1241.00
82_V169_F2.1181.00
25_A290_I2.1071.00
70_N73_F2.1051.00
137_D141_P2.0981.00
112_D195_K2.0911.00
174_L183_L2.0751.00
344_D348_D2.0611.00
424_G428_G2.0561.00
57_S198_Y1.9581.00
277_K318_D1.9441.00
14_N19_T1.9431.00
24_T291_D1.9341.00
83_V221_Y1.9231.00
113_L117_F1.9031.00
61_Q191_L1.8981.00
78_V223_H1.8771.00
309_N445_T1.8531.00
104_V199_I1.8471.00
385_Y389_L1.8431.00
143_S146_N1.8271.00
315_F438_L1.8211.00
310_K381_N1.7931.00
43_L58_F1.7701.00
7_P10_D1.7481.00
280_L331_L1.7461.00
181_S184_S1.7141.00
13_I18_D1.7071.00
314_V441_E1.7031.00
14_N18_D1.6761.00
95_D98_A1.6731.00
47_N53_K1.6701.00
189_N192_N1.6651.00
169_F194_F1.6601.00
13_I19_T1.6571.00
18_D22_T1.6171.00
25_A438_L1.6171.00
133_Y136_G1.6131.00
79_I170_G1.6081.00
86_L211_F1.6051.00
173_K177_K1.6021.00
261_G265_A1.6011.00
81_S221_Y1.5971.00
45_S53_K1.5941.00
289_K336_K1.5751.00
308_I311_A1.5731.00
177_K193_Y1.5721.00
194_F197_L1.5641.00
79_I82_V1.5641.00
58_F213_L1.5601.00
142_D145_R1.5561.00
55_T200_K1.5551.00
80_D223_H1.5491.00
333_L380_F1.5341.00
280_L288_T1.5291.00
222_Y225_D1.5261.00
43_L102_L1.4931.00
308_I442_I1.4861.00
299_D302_K1.4831.00
301_L304_D1.4751.00
167_D170_G1.4741.00
138_L141_P1.4661.00
55_T256_E1.4661.00
32_S126_N1.4581.00
341_P344_D1.4551.00
118_D121_S1.4431.00
30_L287_Y1.4261.00
316_Y377_Y1.4211.00
197_L218_L1.4191.00
268_L271_N1.4111.00
59_Y107_L1.3981.00
100_Q207_A1.3831.00
60_T199_I1.3741.00
80_D164_P1.3651.00
105_Y198_Y1.3601.00
87_P216_S1.3521.00
79_I175_L1.3511.00
258_D261_G1.3501.00
335_D338_N1.3481.00
74_G77_P1.3441.00
314_V379_T1.3401.00
272_N275_S1.3371.00
317_V320_D1.3281.00
419_R423_Y1.3251.00
271_N275_S1.3241.00
88_Y159_P1.3201.00
342_L349_N1.3081.00
40_T89_D1.3041.00
317_V367_L1.2961.00
26_Y291_D1.2951.00
301_L305_N1.2891.00
177_K180_S1.2881.00
368_D377_Y1.2801.00
59_Y254_H1.2771.00
417_P420_T1.2701.00
161_I211_F1.2681.00
357_S360_A1.2681.00
59_Y198_Y1.2641.00
54_T257_H1.2631.00
41_N58_F1.2581.00
259_Y262_T1.2551.00
418_L422_L1.2531.00
168_S172_Q1.2521.00
61_Q112_D1.2511.00
74_G224_Y1.2511.00
278_L438_L1.2461.00
245_I251_Q1.2411.00
42_L208_L1.2411.00
245_I249_V1.2371.00
331_L337_D1.2341.00
35_T125_S1.2301.00
313_L382_I1.2271.00
60_T197_L1.2201.00
16_T19_T1.2191.00
343_N350_T1.1961.00
273_N276_S1.1961.00
174_L194_F1.1751.00
104_V163_I1.1731.00
120_D123_Y1.1731.00
106_E195_K1.1711.00
271_N276_S1.1701.00
42_L283_G1.1691.00
165_L218_L1.1681.00
79_I167_D1.1661.00
136_G141_P1.1641.00
270_S273_N1.1621.00
53_K258_D1.1591.00
267_A270_S1.1561.00
405_G410_P1.1501.00
84_L104_V1.1501.00
63_R191_L1.1461.00
343_N348_D1.1381.00
177_K183_L1.1321.00
57_S200_K1.1321.00
130_S133_Y1.1261.00
137_D140_V1.1201.00
62_L218_L1.1151.00
364_G368_D1.1111.00
34_Q122_T1.1101.00
28_V289_K1.1101.00
245_I250_A1.1071.00
117_F123_Y1.1051.00
246_T249_V1.1041.00
258_D262_T1.1021.00
131_K134_T1.0931.00
85_V162_R1.0921.00
38_L41_N1.0921.00
184_S189_N1.0861.00
173_K193_Y1.0861.00
270_S274_D1.0821.00
358_F361_S1.0821.00
198_Y201_A1.0811.00
419_R424_G1.0781.00
417_P421_V1.0761.00
314_V439_K1.0691.00
56_A255_F1.0621.00
187_N191_L1.0561.00
221_Y224_Y1.0521.00
363_F367_L1.0501.00
353_D356_T1.0401.00
388_N392_G1.0351.00
170_G175_L1.0351.00
364_G369_R1.0351.00
51_F279_Y1.0321.00
18_D21_F1.0271.00
365_G369_R1.0251.00
406_L409_I1.0221.00
318_D321_T1.0081.00
343_N347_E1.0081.00
212_N215_N1.0041.00
227_T231_D1.0001.00
313_L440_L0.9951.00
149_T152_T0.9921.00
142_D148_D0.9881.00
417_P423_Y0.9811.00
345_F349_N0.9811.00
410_P414_T0.9801.00
218_L222_Y0.9751.00
264_I268_L0.9751.00
174_L220_I0.9721.00
77_P224_Y0.9701.00
412_S415_T0.9671.00
140_V145_R0.9641.00
85_V217_K0.9591.00
386_I442_I0.9560.99
71_P74_G0.9550.99
333_L337_D0.9520.99
104_V197_L0.9450.99
84_L199_I0.9400.99
409_I413_S0.9390.99
100_Q201_A0.9390.99
268_L272_N0.9380.99
290_I313_L0.9370.99
263_P266_N0.9370.99
364_G367_L0.9360.99
438_L441_E0.9350.99
19_T22_T0.9280.99
62_L197_L0.9250.99
144_I147_V0.9250.99
3_S6_I0.9240.99
80_D83_V0.9230.99
264_I267_A0.9210.99
342_L348_D0.9210.99
168_S173_K0.9200.99
31_D126_N0.9170.99
41_N213_L0.9170.99
104_V165_L0.9160.99
331_L334_Y0.9160.99
265_A269_L0.9150.99
269_L273_N0.9130.99
410_P413_S0.9120.99
400_P404_L0.9100.99
290_I315_F0.9090.99
157_L160_Q0.9070.99
219_T242_D0.9010.99
68_S71_P0.8980.99
270_S275_S0.8960.99
107_L113_L0.8960.99
267_A279_Y0.8930.99
417_P422_L0.8900.99
48_D279_Y0.8890.99
81_S164_P0.8880.99
221_Y225_D0.8850.99
150_S153_V0.8830.99
102_L201_A0.8780.99
334_Y337_D0.8780.99
319_N322_Y0.8770.99
308_I386_I0.8770.99
95_D205_D0.8750.99
356_T360_A0.8740.99
86_L216_S0.8730.99
182_E189_N0.8730.99
263_P267_A0.8730.99
342_L347_E0.8720.99
300_E304_D0.8700.99
332_L335_D0.8700.99
169_F173_K0.8700.99
228_D231_D0.8690.99
267_A271_N0.8680.99
234_D237_F0.8670.99
23_V313_L0.8620.99
42_L210_Y0.8600.99
348_D352_S0.8590.99
308_I390_L0.8590.99
311_A386_I0.8560.99
82_V165_L0.8550.99
355_T358_F0.8530.99
315_F408_L0.8520.99
426_N429_S0.8520.99
151_T154_T0.8510.99
132_S135_L0.8510.99
91_Y96_T0.8490.99
248_G251_Q0.8470.99
25_A288_T0.8450.99
433_N436_K0.8420.99
167_D172_Q0.8390.99
43_L211_F0.8370.99
2_G5_L0.8330.99
190_F194_F0.8270.99
349_N352_S0.8270.99
337_D341_P0.8250.98
272_N276_S0.8250.98
228_D232_T0.8210.98
6_I9_D0.8190.98
27_T278_L0.8190.98
331_L335_D0.8180.98
164_P221_Y0.8170.98
42_L253_N0.8150.98
343_N346_F0.8150.98
267_A275_S0.8130.98
302_K305_N0.8080.98
341_P348_D0.8080.98
418_L421_V0.8070.98
59_Y252_F0.8060.98
213_L216_S0.8060.98
15_V19_T0.8060.98
153_V156_R0.8060.98
102_L199_I0.8060.98
84_L165_L0.8050.98
289_K405_G0.8020.98
62_L194_F0.7980.98
418_L423_Y0.7980.98
210_Y417_P0.7970.98
23_V290_I0.7970.98
266_N269_L0.7950.98
165_L170_G0.7950.98
382_I385_Y0.7930.98
243_F246_T0.7930.98
434_P437_R0.7920.98
22_T293_P0.7920.98
77_P175_L0.7920.98
402_E405_G0.7920.98
389_L392_G0.7890.98
346_F349_N0.7890.98
368_D371_N0.7880.98
102_L161_I0.7830.98
343_N349_N0.7820.98
269_L272_N0.7800.98
387_Q391_N0.7800.98
345_F351_L0.7800.98
267_A276_S0.7770.98
22_T298_G0.7770.98
143_S148_D0.7750.98
427_P430_T0.7750.98
148_D152_T0.7710.98
259_Y286_V0.7690.98
271_N274_D0.7690.98
106_E169_F0.7660.98
207_A211_F0.7660.98
56_A208_L0.7650.98
230_T234_D0.7640.98
178_D182_E0.7630.98
176_D179_G0.7630.98
112_D192_N0.7630.98
317_V322_Y0.7620.97
348_D351_L0.7610.97
425_S428_G0.7580.97
13_I16_T0.7560.97
45_S201_A0.7560.97
170_G173_K0.7550.97
28_V287_Y0.7520.97
399_N402_E0.7500.97
330_R333_L0.7470.97
12_N15_V0.7460.97
138_L142_D0.7440.97
169_F174_L0.7430.97
229_T232_T0.7420.97
332_L337_D0.7400.97
82_V167_D0.7390.97
266_N270_S0.7350.97
145_R149_T0.7340.97
57_S201_A0.7340.97
98_A205_D0.7320.97
86_L163_I0.7300.97
401_D410_P0.7300.97
68_S73_F0.7270.97
365_G372_D0.7260.97
338_N341_P0.7230.97
295_D298_G0.7220.97
307_L387_Q0.7220.97
340_T343_N0.7220.97
424_G429_S0.7210.96
306_I387_Q0.7200.96
344_D347_E0.7160.96
420_T425_S0.7120.96
145_R148_D0.7110.96
331_L380_F0.7100.96
267_A274_D0.7100.96
150_S154_T0.7090.96
148_D151_T0.7080.96
407_L410_P0.7080.96
90_S210_Y0.7070.96
12_N16_T0.7070.96
341_P346_F0.7060.96
262_T265_A0.7060.96
57_S199_I0.7040.96
119_E125_S0.7020.96
149_T153_V0.7020.96
264_I270_S0.7000.96
322_Y325_S0.7000.96
246_T250_A0.6990.96
356_T359_L0.6990.96
152_T155_T0.6990.96
133_Y137_D0.6980.96
147_V150_S0.6950.96
175_L179_G0.6930.96
345_F348_D0.6920.96
226_T231_D0.6920.96
88_Y161_I0.6880.95
255_F283_G0.6870.95
416_S419_R0.6860.95
130_S135_L0.6850.95
136_G145_R0.6840.95
91_Y210_Y0.6830.95
342_L350_T0.6820.95
267_A272_N0.6820.95
46_Y54_T0.6810.95
421_V424_G0.6790.95
342_L346_F0.6780.95
229_T233_V0.6770.95
34_Q37_N0.6760.95
176_D180_S0.6760.95
249_V252_F0.6750.95
55_T258_D0.6750.95
247_S250_A0.6750.95
424_G427_P0.6740.95
136_G142_D0.6720.95
82_V164_P0.6710.95
107_L110_L0.6690.95
140_V144_I0.6690.95
129_P133_Y0.6680.95
263_P268_L0.6660.95
372_D375_N0.6650.95
219_T222_Y0.6620.94
87_P215_N0.6610.94
131_K135_L0.6590.94
405_G409_I0.6590.94
172_Q175_L0.6580.94
166_D172_Q0.6570.94
384_R387_Q0.6560.94
319_N376_T0.6560.94
422_L425_S0.6560.94
299_D303_N0.6530.94
106_E110_L0.6520.94
69_S187_N0.6500.94
421_V425_S0.6480.94
369_R372_D0.6460.94
122_T126_N0.6450.94
144_I148_D0.6450.94
206_G209_L0.6450.94
256_E259_Y0.6420.93
350_T353_D0.6410.93
354_N357_S0.6410.93
133_Y138_L0.6380.93
64_L69_S0.6370.93
324_N327_P0.6360.93
89_D210_Y0.6360.93
346_F350_T0.6340.93
266_N272_N0.6340.93
357_S361_S0.6330.93
385_Y388_N0.6330.93
230_T235_D0.6290.93
80_D162_R0.6290.93
130_S136_G0.6290.93
265_A268_L0.6280.93
260_S265_A0.6280.93
384_R388_N0.6280.93
41_N251_Q0.6270.93
8_D13_I0.6270.93
244_S249_V0.6260.93
335_D341_P0.6250.93
228_D234_D0.6240.92
50_V279_Y0.6230.92
427_P433_N0.6230.92
405_G411_V0.6230.92
257_H260_S0.6220.92
21_F24_T0.6200.92
88_Y207_A0.6160.92
131_K137_D0.6160.92
354_N358_F0.6160.92
332_L336_K0.6160.92
432_G436_K0.6160.92
54_T255_F0.6160.92
317_V321_T0.6150.92
288_T291_D0.6140.92
368_D376_T0.6140.92
81_S223_H0.6130.92
367_L370_D0.6120.92
159_P162_R0.6110.92
103_S315_F0.6100.92
207_A210_Y0.6090.92
402_E406_L0.6090.92
105_Y202_D0.6090.92
54_T281_Q0.6080.91
125_S284_A0.6070.91
425_S429_S0.6060.91
257_H281_Q0.6040.91
261_G264_I0.6030.91
231_D234_D0.6030.91
83_V219_T0.6020.91
423_Y426_N0.6020.91
35_T252_F0.6010.91
48_D419_R0.6010.91
290_I333_L0.6010.91
269_L274_D0.6010.91
347_E357_S0.6000.91
93_Y210_Y0.6000.91
264_I269_L0.6000.91
283_G380_F0.5950.91
142_D152_T0.5940.91
86_L161_I0.5940.91
420_T424_G0.5930.90
282_G334_Y0.5910.90
88_Y190_F0.5910.90
154_T157_L0.5900.90
8_D16_T0.5880.90
411_V414_T0.5850.90
424_G430_T0.5850.90
23_V292_L0.5850.90
300_E303_N0.5850.90
330_R334_Y0.5790.89
422_L429_S0.5790.89
233_V236_T0.5770.89
248_G252_F0.5760.89
60_T64_L0.5740.89
232_T237_F0.5730.89
225_D229_T0.5730.89
265_A270_S0.5720.89
230_T237_F0.5710.89
132_S138_L0.5700.89
383_T390_L0.5700.89
40_T210_Y0.5700.89
170_G180_S0.5690.89
278_L315_F0.5680.88
71_P174_L0.5680.88
398_K401_D0.5650.88
259_Y281_Q0.5650.88
319_N323_D0.5650.88
181_S189_N0.5640.88
358_F362_Y0.5630.88
308_I444_Y0.5620.88
21_F292_L0.5610.88
7_P12_N0.5610.88
179_G189_N0.5600.88
279_Y370_D0.5590.88
147_V151_T0.5590.88
142_D147_V0.5580.88
300_E305_N0.5580.88
70_N75_D0.5570.88
47_N205_D0.5560.87
316_Y376_T0.5540.87
12_N18_D0.5520.87
386_I390_L0.5520.87
432_G435_D0.5520.87
61_Q250_A0.5510.87
415_T418_L0.5510.87
135_L140_V0.5500.87
264_I272_N0.5490.87
233_V237_F0.5490.87
318_D423_Y0.5480.87
333_L336_K0.5470.87
423_Y427_P0.5470.87
169_F204_G0.5440.86
289_K407_L0.5430.86
216_S219_T0.5430.86
268_L273_N0.5430.86
12_N17_T0.5430.86
316_Y436_K0.5420.86
399_N403_N0.5410.86
139_T145_R0.5410.86
139_T142_D0.5410.86
147_V154_T0.5410.86
293_P380_F0.5410.86
151_T155_T0.5390.86
264_I274_D0.5390.86
141_P145_R0.5380.86
117_F124_Y0.5370.86
73_F77_P0.5360.85
383_T387_Q0.5360.85
319_N324_N0.5350.85
355_T360_A0.5340.85
17_T168_S0.5340.85
60_T216_S0.5330.85
142_D149_T0.5330.85
255_F284_A0.5320.85
111_E121_S0.5320.85
135_L141_P0.5320.85
316_Y319_N0.5310.85
329_S332_L0.5310.85
344_D349_N0.5310.85
173_K176_D0.5300.85
269_L275_S0.5300.85
322_Y326_Y0.5300.85
178_D181_S0.5300.85
428_G432_G0.5280.85
63_R187_N0.5280.85
104_V191_L0.5270.85
146_N149_T0.5270.85
84_L197_L0.5260.84
218_L224_Y0.5260.84
180_S183_L0.5250.84
347_E350_T0.5230.84
426_N430_T0.5230.84
32_S124_Y0.5230.84
320_D327_P0.5230.84
217_K242_D0.5220.84
435_D440_L0.5220.84
263_P276_S0.5220.84
175_L220_I0.5200.84
278_L286_V0.5190.84
32_S35_T0.5190.84
48_D281_Q0.5170.83
173_K178_D0.5170.83
8_D14_N0.5170.83
341_P350_T0.5160.83
363_F369_R0.5160.83
225_D230_T0.5150.83
85_V157_L0.5150.83
346_F351_L0.5150.83
247_S251_Q0.5140.83
78_V221_Y0.5130.83
83_V167_D0.5130.83
246_T251_Q0.5120.83
65_P190_F0.5110.83
79_I164_P0.5100.83
329_S337_D0.5100.83
24_T28_V0.5100.83
187_N190_F0.5100.83
112_D191_L0.5090.82
128_D254_H0.5090.82
216_S222_Y0.5080.82
297_I386_I0.5060.82
79_I309_N0.5050.82
144_I150_S0.5050.82
360_A365_G0.5040.82
134_T138_L0.5030.82
359_L364_G0.5020.82
123_Y126_N0.5010.81
73_F178_D0.5010.81
367_L371_N0.5000.81
411_V415_T0.5000.81
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4cbuG 1 0.2601 8.3 0.969 Contact Map
2vikA 1 0.2646 7.1 0.97 Contact Map
3glwZ 2 0.0381 2.4 0.976 Contact Map
1t44G 1 0.3027 1.4 0.979 Contact Map
2mdvA 2 0.0852 1.3 0.979 Contact Map
2r0jA 1 0.1278 0.9 0.982 Contact Map
2fo3A 1 0.1233 0.8 0.982 Contact Map
3fm7C 1 0.0493 0.8 0.982 Contact Map
1m45B 1 0.0561 0.7 0.983 Contact Map
1bazA 4 0.1121 0.7 0.983 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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