GREMLIN Database
DUF4258 - Domain of unknown function (DUF4258)
PFAM: PF14076 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 63 (62)
Sequences: 22772 (17796)
Seq/√Len: 2260.1
META: 0.968

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
8_R12_R3.5171.00
15_S18_D2.6651.00
5_A58_V2.4451.00
8_R11_E2.3541.00
21_E25_N2.2661.00
6_L16_E1.9951.00
46_H62_Y1.8201.00
1_F16_E1.7031.00
3_K7_E1.6351.00
2_T57_V1.6001.00
52_D57_V1.5951.00
16_E20_E1.4961.00
1_F19_V1.4731.00
22_A45_L1.4711.00
20_E24_L1.4701.00
9_M61_V1.4511.00
7_E11_E1.4241.00
29_I32_Y1.4061.00
1_F6_L1.4051.00
34_L37_G1.2981.00
22_A47_V1.2171.00
1_F5_A1.1981.00
6_L9_M1.1541.00
36_L44_P1.1261.00
53_E56_L1.0821.00
50_A59_I1.0571.00
14_I45_L1.0411.00
50_A55_P1.0361.00
51_I56_L1.0061.00
38_K44_P1.0011.00
36_L46_H0.9971.00
9_M19_V0.9971.00
29_I33_P0.9961.00
3_K6_L0.9791.00
52_D55_P0.9691.00
7_E10_A0.9561.00
28_I31_D0.9521.00
14_I61_V0.9471.00
49_V58_V0.9381.00
49_V56_L0.9351.00
1_F20_E0.9231.00
17_E21_E0.9181.00
6_L10_A0.8981.00
23_L26_G0.8941.00
36_L49_V0.8921.00
52_D56_L0.8821.00
30_E33_P0.8701.00
39_T43_R0.8121.00
18_D21_E0.8011.00
22_A26_G0.8001.00
18_D40_I0.7991.00
5_A61_V0.7981.00
22_A35_V0.7941.00
17_E20_E0.7851.00
23_L58_V0.7481.00
21_E40_I0.7341.00
28_I32_Y0.7341.00
34_L38_K0.7321.00
34_L39_T0.7101.00
40_I43_R0.7081.00
48_V59_I0.7051.00
37_G46_H0.7001.00
1_F9_M0.6981.00
8_R14_I0.6901.00
9_M16_E0.6731.00
50_A53_E0.6601.00
8_R61_V0.6591.00
32_Y46_H0.6511.00
46_H50_A0.6331.00
23_L47_V0.6261.00
47_V51_I0.6231.00
19_V23_L0.6181.00
4_H60_T0.6121.00
8_R62_Y0.6091.00
4_H8_R0.6051.00
47_V50_A0.6031.00
48_V51_I0.6021.00
18_D37_G0.5991.00
34_L46_H0.5971.00
48_V62_Y0.5941.00
19_V47_V0.5761.00
38_K42_G0.5751.00
50_A57_V0.5531.00
3_K10_A0.5501.00
3_K11_E0.5481.00
37_G40_I0.5481.00
26_G35_V0.5281.00
8_R13_G0.5261.00
19_V61_V0.5241.00
21_E24_L0.5071.00
51_I57_V0.5021.00
10_A16_E0.5011.00
49_V52_D0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3u97A 3 1 36.1 0.769 Contact Map
1rfzA 4 0.9683 13.3 0.812 Contact Map
1tlqA 4 0.9683 12.5 0.814 Contact Map
1y9iA 4 0.9683 11.3 0.818 Contact Map
2fhzB 1 0.9841 10.5 0.82 Contact Map
1y6xA 3 0.3651 9.2 0.825 Contact Map
4muqA 1 0.9841 8.1 0.829 Contact Map
2xvsA 1 1 7.4 0.833 Contact Map
2hx6A 1 0.9841 7.2 0.833 Contact Map
4dn7A 2 0.8571 6.4 0.837 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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