GREMLIN Database
DUF4256 - Protein of unknown function (DUF4256)
PFAM: PF14066 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 173 (173)
Sequences: 6137 (2598)
Seq/√Len: 197.5
META: 0.899

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
80_R97_D3.9041.00
129_V144_C3.8351.00
104_I169_G3.6411.00
80_R84_E3.5631.00
114_E117_K3.4001.00
40_E43_R3.2191.00
25_Q29_E3.1311.00
144_C153_V3.0761.00
51_V63_Y2.8421.00
94_S97_D2.7311.00
76_L93_N2.6161.00
76_L98_M2.5041.00
135_I150_T2.4881.00
93_N97_D2.4801.00
146_R149_D2.4011.00
20_E23_K2.3981.00
134_D149_D2.3091.00
120_E146_R2.2731.00
83_L94_S2.2461.00
111_Q170_S2.1991.00
6_K10_E2.1901.00
134_D150_T2.1811.00
105_E130_K2.1421.00
123_T146_R2.1031.00
142_L153_V2.1021.00
145_D154_Y2.0781.00
129_V142_L2.0451.00
53_Y58_G1.9291.00
6_K25_Q1.9141.00
83_L90_K1.9101.00
68_E123_T1.7961.00
68_E124_K1.6681.00
59_E172_R1.6381.00
84_E90_K1.6331.00
93_N101_A1.6231.00
143_F154_Y1.6191.00
113_R150_T1.5921.00
52_G61_I1.5491.00
36_W40_E1.5401.00
96_V106_L1.5341.00
85_S159_E1.5311.00
88_E159_E1.5281.00
95_A106_L1.5101.00
39_N43_R1.4881.00
145_D155_H1.4471.00
36_W39_N1.4361.00
37_S102_M1.4311.00
76_L95_A1.4291.00
19_L24_V1.4271.00
120_E123_T1.4131.00
14_N119_G1.4001.00
69_S164_A1.3891.00
82_A159_E1.3581.00
109_E113_R1.3391.00
63_Y170_S1.3371.00
78_Y130_K1.2861.00
80_R94_S1.2651.00
75_S163_A1.2641.00
51_V65_C1.2211.00
90_K159_E1.2061.00
82_A90_K1.1941.00
130_K133_A1.1861.00
83_L97_D1.1811.00
131_T141_A1.1671.00
38_L62_F1.1451.00
108_T111_Q1.1231.00
53_Y60_Y1.0991.00
93_N98_M1.0941.00
23_K53_Y1.0921.00
85_S90_K1.0631.00
81_E85_S1.0611.00
82_A94_S1.0511.00
97_D101_A1.0341.00
89_H93_N1.0301.00
111_Q114_E1.0161.00
68_E74_R1.0001.00
74_R163_A0.9871.00
34_K59_E0.9821.00
110_E114_E0.9600.99
138_L150_T0.9510.99
135_I142_L0.9510.99
120_E149_D0.9490.99
38_L171_L0.9480.99
148_Y157_G0.9410.99
52_G114_E0.9290.99
15_R49_D0.9160.99
133_A137_K0.9100.99
74_R125_T0.9050.99
108_T130_K0.8960.99
129_V153_V0.8850.99
132_P135_I0.8790.99
71_K75_S0.8780.99
37_S171_L0.8760.99
113_R143_F0.8730.99
54_D57_T0.8680.99
17_K55_K0.8640.99
138_L149_D0.8630.99
80_R93_N0.8620.99
51_V170_S0.8600.99
118_L121_F0.8590.99
148_Y164_A0.8490.99
69_S75_S0.8380.98
89_H161_Y0.8370.98
144_C150_T0.8360.98
113_R153_V0.8210.98
63_Y114_E0.8030.98
16_H49_D0.8030.98
110_E117_K0.7960.98
16_H50_V0.7900.98
109_E150_T0.7880.98
22_A26_A0.7870.98
102_M169_G0.7800.97
83_L93_N0.7780.97
90_K161_Y0.7760.97
95_A99_A0.7710.97
3_K6_K0.7670.97
157_G160_S0.7620.97
22_A25_Q0.7610.97
45_G71_K0.7610.97
155_H162_Y0.7600.97
69_S125_T0.7580.97
27_K58_G0.7560.97
71_K88_E0.7540.97
69_S74_R0.7450.97
63_Y107_L0.7370.96
134_D138_L0.7360.96
87_K161_Y0.7340.96
41_M169_G0.7340.96
61_I172_R0.7300.96
23_K26_A0.7270.96
105_E111_Q0.7230.96
26_A29_E0.7140.96
144_C159_E0.7110.96
75_S88_E0.7040.95
88_E92_K0.6970.95
98_M101_A0.6940.95
96_V100_A0.6940.95
134_D137_K0.6920.95
40_E102_M0.6900.95
54_D172_R0.6900.95
76_L82_A0.6840.95
36_W43_R0.6810.95
23_K60_Y0.6770.94
27_K53_Y0.6740.94
61_I170_S0.6660.94
87_K90_K0.6650.94
143_F162_Y0.6630.94
84_E97_D0.6610.94
77_C86_R0.6600.94
71_K89_H0.6600.94
144_C151_V0.6560.93
24_V60_Y0.6520.93
55_K58_G0.6510.93
113_R154_Y0.6500.93
13_M18_G0.6500.93
143_F148_Y0.6460.93
78_Y142_L0.6450.93
1_L4_T0.6450.93
83_L161_Y0.6370.92
108_T170_S0.6350.92
94_S98_M0.6330.92
81_E137_K0.6300.92
67_A123_T0.6290.92
108_T129_V0.6230.91
37_S40_E0.6220.91
31_N34_K0.6210.91
27_K60_Y0.6200.91
75_S89_H0.6190.91
33_E36_W0.6190.91
56_K92_K0.6180.91
60_Y173_V0.6170.91
95_A164_A0.6150.91
139_G159_E0.6150.91
68_E125_T0.6140.91
37_S104_I0.6120.91
89_H159_E0.6110.91
14_N120_E0.6110.91
155_H160_S0.6100.91
160_S163_A0.6070.90
55_K61_I0.6070.90
85_S89_H0.6010.90
3_K7_A0.6010.90
81_E84_E0.6010.90
157_G161_Y0.5970.90
84_E159_E0.5950.90
23_K56_K0.5920.89
23_K58_G0.5920.89
148_Y159_E0.5900.89
147_R155_H0.5900.89
98_M167_F0.5870.89
126_S154_Y0.5860.89
4_T39_N0.5850.89
9_F16_H0.5850.89
76_L83_L0.5730.88
82_A89_H0.5710.88
159_E164_A0.5690.87
88_E161_Y0.5670.87
75_S83_L0.5610.87
84_E94_S0.5610.87
12_N49_D0.5580.86
64_D73_R0.5560.86
80_R101_A0.5520.86
93_N96_V0.5500.86
146_R151_V0.5480.85
13_M20_E0.5470.85
78_Y96_V0.5470.85
53_Y57_T0.5470.85
3_K25_Q0.5460.85
92_K159_E0.5450.85
2_L35_L0.5420.85
77_C83_L0.5420.85
74_R113_R0.5400.85
113_R144_C0.5400.85
62_F173_V0.5390.84
81_E159_E0.5390.84
93_N161_Y0.5390.84
159_E163_A0.5380.84
107_L170_S0.5360.84
148_Y154_Y0.5350.84
80_R85_S0.5350.84
13_M19_L0.5330.84
46_G64_D0.5320.84
152_F156_N0.5310.84
68_E71_K0.5310.84
123_T149_D0.5300.83
63_Y111_Q0.5290.83
76_L92_K0.5280.83
7_A110_E0.5260.83
12_N16_H0.5250.83
79_D82_A0.5240.83
67_A119_G0.5240.83
68_E120_E0.5220.83
87_K126_S0.5220.83
71_K74_R0.5210.82
126_S161_Y0.5210.82
115_L119_G0.5190.82
117_K121_F0.5170.82
113_R129_V0.5170.82
6_K22_A0.5150.82
96_V130_K0.5140.82
131_T144_C0.5130.81
7_A11_K0.5130.81
27_K57_T0.5100.81
52_G118_L0.5100.81
83_L89_H0.5100.81
46_G73_R0.5080.81
85_S161_Y0.5070.81
10_E13_M0.5070.81
34_K43_R0.5060.81
54_D118_L0.5060.81
162_Y165_R0.5060.81
12_N15_R0.5050.80
105_E170_S0.5050.80
105_E108_T0.5050.80
106_L126_S0.5030.80
109_E129_V0.5030.80
63_Y119_G0.5030.80
81_E133_A0.5020.80
52_G58_G0.5010.80
143_F155_H0.5000.80
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2y8tB 1 0.2081 34.5 0.959 Contact Map
1o7bT 1 0.5145 33.3 0.96 Contact Map
4recA 2 0.5376 31 0.96 Contact Map
1ob8A 1 0.7283 26.9 0.961 Contact Map
2eo0A 2 0.6994 18.3 0.964 Contact Map
1gefA 2 0.5723 17 0.965 Contact Map
1xmxA 2 0.7225 16.3 0.965 Contact Map
3pjxA 1 0.659 12.9 0.967 Contact Map
1dgsA 1 0.1329 12.8 0.967 Contact Map
4otmA 2 0.3526 12.2 0.967 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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