GREMLIN Database
DUF4255 - Protein of unknown function (DUF4255)
PFAM: PF14065 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 178 (174)
Sequences: 9735 (8003)
Seq/√Len: 606.7
META: 0.904

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
51_N77_D4.1211.00
41_L101_A3.8611.00
82_L101_A3.5991.00
50_E55_R3.1471.00
7_R11_R3.1371.00
138_E142_K3.1011.00
112_P115_L3.0181.00
77_D158_V2.9521.00
45_L78_L2.5511.00
130_I159_V2.3011.00
74_L102_M2.2791.00
4_A7_R2.2591.00
13_A104_V2.2531.00
41_L84_A2.0411.00
136_S139_E1.9951.00
8_D11_R1.9731.00
80_Y95_H1.9141.00
43_L82_L1.8881.00
46_Y156_A1.8471.00
128_L159_V1.8121.00
151_Y154_S1.7681.00
108_H115_L1.7581.00
8_D12_D1.7201.00
3_T95_H1.7051.00
79_H158_V1.7041.00
42_N83_T1.6611.00
102_M155_V1.6311.00
63_R67_T1.5821.00
105_L157_Y1.5541.00
81_L156_A1.5531.00
102_M157_Y1.5501.00
2_V78_L1.5121.00
11_R15_P1.4961.00
51_N54_L1.4831.00
47_R79_H1.4831.00
49_R79_H1.4831.00
79_H133_E1.4811.00
107_D166_S1.4731.00
101_A155_V1.4581.00
75_A163_L1.4501.00
9_L13_A1.4391.00
133_E158_V1.4271.00
172_A175_P1.4231.00
139_E142_K1.3621.00
62_R70_R1.3521.00
84_A101_A1.3481.00
3_T25_F1.3381.00
98_L153_P1.3271.00
100_R103_Q1.2951.00
9_L108_H1.2881.00
121_L124_Q1.2851.00
83_T154_S1.2831.00
103_Q164_I1.2821.00
45_L80_Y1.2761.00
141_S145_S1.2671.00
44_F151_Y1.2591.00
135_L156_A1.2381.00
74_L106_H1.2371.00
4_A8_D1.2261.00
60_P70_R1.2251.00
5_T117_P1.1981.00
3_T80_Y1.1781.00
26_D30_D1.1771.00
12_D15_P1.1721.00
12_D115_L1.1671.00
100_R104_V1.1601.00
90_E93_E1.1591.00
25_F28_P1.1571.00
119_S122_A1.1351.00
132_L155_V1.1351.00
9_L104_V1.1221.00
24_S42_N1.1161.00
30_D33_A1.1151.00
80_Y159_V1.1141.00
76_L102_M1.1131.00
11_R22_D1.1111.00
49_R77_D1.0891.00
46_Y79_H1.0811.00
18_V21_V1.0801.00
31_D35_S1.0791.00
33_A36_L1.0641.00
48_V78_L1.0541.00
42_N85_W1.0521.00
143_L147_L1.0441.00
83_T152_R1.0361.00
66_G69_R1.0311.00
71_R168_R1.0241.00
10_L43_L1.0211.00
23_V28_P1.0041.00
7_R43_L1.0021.00
5_T9_L0.9971.00
63_R68_T0.9971.00
77_D160_R0.9921.00
55_R58_P0.9841.00
131_T158_V0.9801.00
41_L97_L0.9761.00
45_L48_V0.9731.00
6_L43_L0.9711.00
10_L101_A0.9641.00
44_F83_T0.9581.00
122_A125_I0.9571.00
129_R160_R0.9551.00
141_S146_A0.9531.00
70_R165_E0.9511.00
93_E96_R0.9291.00
29_D33_A0.9281.00
103_Q107_D0.9261.00
46_Y143_L0.9191.00
27_P30_D0.9181.00
114_D117_P0.9081.00
45_L95_H0.9081.00
137_L141_S0.9081.00
48_V98_L0.8891.00
25_F31_D0.8851.00
112_P127_P0.8671.00
19_S22_D0.8591.00
22_D40_T0.8551.00
113_R126_E0.8531.00
32_W37_T0.8481.00
113_R127_P0.8461.00
79_H156_A0.8451.00
50_E132_L0.8421.00
12_D16_A0.8371.00
120_D125_I0.8351.00
88_D91_D0.8321.00
54_L57_A0.8261.00
21_V25_F0.8171.00
8_D117_P0.8001.00
41_L82_L0.7981.00
133_E156_A0.7951.00
113_R116_L0.7941.00
32_W36_L0.7941.00
28_P31_D0.7921.00
120_D123_D0.7881.00
94_E152_R0.7851.00
142_K145_S0.7771.00
21_V24_S0.7751.00
98_L155_V0.7691.00
46_Y154_S0.7591.00
64_D68_T0.7531.00
20_G24_S0.7521.00
81_L154_S0.7511.00
110_V129_R0.7471.00
171_R174_P0.7421.00
84_A153_P0.7401.00
104_V108_H0.7381.00
173_A176_V0.7371.00
64_D67_T0.7361.00
124_Q128_L0.7351.00
135_L139_E0.7351.00
31_D36_L0.7341.00
31_D34_A0.7291.00
47_R140_L0.7251.00
15_P21_V0.7241.00
81_L147_L0.7241.00
5_T116_L0.7221.00
114_D118_G0.7201.00
110_V127_P0.7191.00
10_L23_V0.7171.00
85_W152_R0.7171.00
27_P31_D0.7151.00
59_L63_R0.7101.00
30_D34_A0.7101.00
170_P173_A0.7091.00
117_P122_A0.7041.00
137_L140_L0.6901.00
120_D124_Q0.6861.00
7_R23_V0.6841.00
86_G94_E0.6771.00
124_Q129_R0.6741.00
24_S28_P0.6701.00
34_A37_T0.6671.00
47_R133_E0.6661.00
84_A98_L0.6661.00
169_P172_A0.6651.00
146_A151_Y0.6641.00
63_R66_G0.6631.00
21_V26_D0.6581.00
23_V27_P0.6521.00
24_S40_T0.6491.00
62_R65_G0.6491.00
20_G28_P0.6491.00
113_R122_A0.6441.00
74_L109_P0.6441.00
136_S142_K0.6431.00
133_E139_E0.6421.00
53_A57_A0.6401.00
63_R69_R0.6401.00
23_V26_D0.6391.00
34_A39_P0.6361.00
105_L130_I0.6351.00
91_D95_H0.6351.00
5_T8_D0.6341.00
17_L100_R0.6341.00
7_R25_F0.6331.00
107_D168_R0.6331.00
65_G68_T0.6311.00
147_L151_Y0.6291.00
18_V22_D0.6281.00
65_G69_R0.6261.00
22_D38_A0.6261.00
7_R16_A0.6241.00
126_E130_I0.6181.00
50_E75_A0.6171.00
84_A94_E0.6101.00
13_A16_A0.6101.00
138_E151_Y0.6091.00
44_F152_R0.6061.00
28_P33_A0.6041.00
29_D32_W0.6041.00
108_H112_P0.6001.00
86_G97_L0.5991.00
24_S29_D0.5831.00
54_L73_P0.5771.00
11_R20_G0.5681.00
26_D34_A0.5681.00
39_P91_D0.5661.00
48_V76_L0.5641.00
134_P158_V0.5621.00
24_S27_P0.5591.00
15_P19_S0.5591.00
20_G25_F0.5581.00
73_P165_E0.5571.00
121_L126_E0.5561.00
76_L164_I0.5561.00
11_R14_L0.5561.00
36_L39_P0.5530.99
53_A56_N0.5470.99
22_D25_F0.5470.99
99_G103_Q0.5460.99
125_I128_L0.5460.99
25_F29_D0.5410.99
11_R23_V0.5390.99
80_Y130_I0.5370.99
21_V28_P0.5360.99
96_R100_R0.5310.99
88_D93_E0.5290.99
60_P65_G0.5260.99
123_D128_L0.5220.99
78_L162_V0.5200.99
35_S38_A0.5190.99
21_V27_P0.5190.99
6_L9_L0.5170.99
146_A152_R0.5170.99
10_L13_A0.5160.99
14_L17_L0.5160.99
132_L135_L0.5160.99
71_R109_P0.5150.99
103_Q166_S0.5140.99
30_D150_P0.5130.99
62_R67_T0.5120.99
58_P61_R0.5070.99
9_L115_L0.5060.99
169_P174_P0.5050.99
113_R123_D0.5050.99
97_L100_R0.5050.99
34_A38_A0.5040.99
74_L155_V0.5040.99
76_L162_V0.5010.99
32_W40_T0.5000.99
16_A21_V0.5000.99
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4hudA 3 0.8371 52.1 0.895 Contact Map
4e17B 1 0.0843 5.3 0.936 Contact Map
1dp3A 1 0.2584 4.5 0.938 Contact Map
3fz2A 5 0.7022 3.8 0.94 Contact Map
1wyqA 1 0.6573 3 0.943 Contact Map
4qpoA 4 0.2472 2.2 0.947 Contact Map
4lkbF 4 0.236 2.2 0.947 Contact Map
2jnsA 1 0.4831 1.9 0.949 Contact Map
3nw0A 1 0.4438 1.8 0.949 Contact Map
3zuzA 1 0.2921 1.6 0.951 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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