GREMLIN Database
DUF4254 - Protein of unknown function (DUF4254)
PFAM: PF14063 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 144 (142)
Sequences: 4087 (2127)
Seq/√Len: 178.5
META: 0.899

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
94_R98_E3.6541.00
73_T79_A3.0661.00
30_E33_A2.7261.00
104_E108_A2.5751.00
102_A106_H2.4551.00
66_K139_R2.4341.00
94_R118_L2.3021.00
9_W13_V2.2291.00
134_D139_R2.1981.00
111_Q115_A2.1251.00
79_A128_I2.1241.00
126_T130_Q2.0721.00
70_R130_Q2.0691.00
17_L37_R2.0191.00
40_R44_D1.9751.00
51_R54_S1.9471.00
10_I44_D1.8571.00
97_A107_R1.8501.00
119_E143_K1.8331.00
73_T127_A1.8101.00
58_E61_K1.7951.00
87_A121_R1.7481.00
108_A112_A1.7151.00
20_I34_L1.6871.00
13_V41_S1.6631.00
3_L51_R1.5731.00
17_L20_I1.5531.00
134_D140_K1.5521.00
107_R111_Q1.5371.00
112_A115_A1.5261.00
13_V17_L1.5211.00
109_K112_A1.4881.00
17_L34_L1.4841.00
93_M117_L1.4421.00
104_E107_R1.4211.00
133_D137_A1.4021.00
123_D143_K1.3621.00
27_D30_E1.3511.00
133_D136_A1.3441.00
96_E99_R1.3411.00
100_T107_R1.3401.00
70_R140_K1.3171.00
94_R97_A1.2971.00
21_I31_A1.2521.00
17_L41_S1.2461.00
8_H84_S1.2451.00
33_A37_R1.2111.00
43_Q47_D1.1821.00
29_V33_A1.1681.00
79_A127_A1.1591.00
129_D133_D1.1551.00
90_I117_L1.1411.00
106_H110_C1.1161.00
30_E34_L1.1131.00
130_Q134_D1.1061.00
90_I118_L1.1041.00
109_K113_K1.0931.00
93_M113_K1.0921.00
49_V78_W1.0901.00
16_H92_H1.0901.00
10_I48_L1.0891.00
105_E109_K1.0871.00
12_T16_H1.0781.00
23_D30_E1.0751.00
28_P32_L1.0691.00
16_H95_E1.0681.00
3_L6_R1.0471.00
60_L135_I1.0441.00
91_Y95_E1.0361.00
20_I23_D1.0331.00
35_K39_D1.0100.99
12_T88_L1.0030.99
4_L7_K0.9990.99
105_E108_A0.9980.99
103_S107_R0.9950.99
71_I141_Y0.9830.99
108_A111_Q0.9800.99
55_Y59_K0.9770.99
26_I31_A0.9760.99
76_P142_M0.9740.99
48_L51_R0.9730.99
122_A126_T0.9720.99
93_M110_C0.9690.99
27_D32_L0.9650.99
11_D14_Q0.9640.99
90_I114_L0.9540.99
76_P132_L0.9470.99
10_I41_S0.9450.99
119_E122_A0.9430.99
44_D48_L0.9420.99
35_K42_N0.9280.99
7_K11_D0.9250.99
23_D26_I0.9220.99
54_S58_E0.9060.99
8_H11_D0.9020.99
96_E106_H0.9020.99
49_V75_S0.8990.99
51_R57_L0.8950.99
18_E22_R0.8940.99
59_K136_A0.8930.99
115_A118_L0.8910.99
56_F60_L0.8830.98
31_A34_L0.8740.98
71_I127_A0.8690.98
56_F76_P0.8660.98
70_R139_R0.8650.98
28_P31_A0.8600.98
15_W18_E0.8570.98
110_C113_K0.8520.98
92_H95_E0.8380.98
20_I30_E0.8330.98
6_R10_I0.8320.98
70_R134_D0.8240.98
76_P128_I0.8240.98
35_K38_I0.8230.98
118_L121_R0.8170.97
14_Q21_I0.8070.97
102_A110_C0.8020.97
134_D137_A0.7950.97
93_M97_A0.7890.97
104_E111_Q0.7750.96
6_R44_D0.7740.96
14_Q46_T0.7670.96
54_S57_L0.7610.96
133_D139_R0.7550.96
12_T92_H0.7540.96
102_A107_R0.7480.96
132_L136_A0.7450.96
85_I89_K0.7450.96
96_E101_D0.7410.95
69_A141_Y0.7360.95
87_A91_Y0.7330.95
135_I141_Y0.7310.95
86_L116_V0.7280.95
34_L37_R0.7210.95
12_T91_Y0.7180.95
97_A114_L0.7170.95
6_R48_L0.7150.94
56_F142_M0.7090.94
80_I128_I0.7080.94
38_I42_N0.7070.94
128_I132_L0.7060.94
121_R125_S0.6960.94
5_Y12_T0.6950.94
40_R51_R0.6820.93
46_T49_V0.6810.93
115_A119_E0.6760.93
89_K117_L0.6730.92
65_P69_A0.6700.92
16_H91_Y0.6680.92
101_D107_R0.6640.92
72_N78_W0.6620.92
111_Q118_L0.6620.92
70_R133_D0.6610.92
135_I140_K0.6520.91
39_D42_N0.6520.91
26_I32_L0.6500.91
7_K10_I0.6480.91
96_E102_A0.6470.91
90_I121_R0.6440.91
56_F77_A0.6360.90
97_A110_C0.6350.90
18_E42_N0.6230.89
22_R42_N0.6200.89
97_A102_A0.6160.89
43_Q46_T0.6110.88
15_W22_R0.6100.88
96_E128_I0.6060.88
52_I80_I0.6050.88
83_L125_S0.6050.88
57_L142_M0.6020.88
15_W19_D0.5960.87
71_I76_P0.5890.86
22_R39_D0.5860.86
4_L11_D0.5800.86
86_L114_L0.5730.85
3_L48_L0.5730.85
20_I37_R0.5710.85
21_I34_L0.5700.85
3_L55_Y0.5680.84
135_I142_M0.5670.84
4_L52_I0.5650.84
48_L97_A0.5610.84
112_A119_E0.5600.84
33_A40_R0.5600.84
71_I126_T0.5580.83
18_E35_K0.5570.83
29_V32_L0.5550.83
17_L75_S0.5540.83
79_A83_L0.5390.81
7_K48_L0.5380.81
130_Q139_R0.5380.81
11_D21_I0.5370.81
106_H109_K0.5360.81
10_I13_V0.5310.80
99_R106_H0.5310.80
17_L30_E0.5290.80
130_Q140_K0.5280.80
43_Q85_I0.5280.80
78_W86_L0.5270.80
131_L134_D0.5200.79
92_H99_R0.5190.79
27_D34_L0.5190.79
89_K110_C0.5170.79
42_N85_I0.5170.79
10_I14_Q0.5140.78
19_D22_R0.5120.78
64_K68_D0.5080.77
72_N140_K0.5060.77
109_K136_A0.5040.77
22_R35_K0.5030.77
119_E123_D0.5010.76
63_V69_A0.5000.76
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4q4gX 1 0 64.3 0.916 Contact Map
4cgkA 3 0.9792 31.8 0.931 Contact Map
3swyA 3 0.2569 16.6 0.939 Contact Map
3swfA 3 0.2639 15 0.941 Contact Map
1flkA 3 0.4097 8.6 0.947 Contact Map
3pp5A 2 0.3889 2.9 0.958 Contact Map
4n78D 1 0.3472 2.8 0.958 Contact Map
3csxA 2 0.3264 2.7 0.958 Contact Map
3tweA 2 0.1806 2.6 0.958 Contact Map
1b9pA 1 0.2361 2.6 0.958 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.006 seconds.