GREMLIN Database
DUF4250 - Domain of unknown function (DUF4250)
PFAM: PF14056 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 55 (55)
Sequences: 2385 (1811)
Seq/√Len: 244.1
META: 0.789

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
34_K38_E3.0411.00
24_D34_K2.9491.00
15_K19_E2.5101.00
26_L30_L1.9361.00
12_V41_L1.7651.00
20_Y29_D1.7331.00
42_A47_E1.6621.00
15_K30_L1.6621.00
25_E29_D1.6431.00
11_Y14_T1.6071.00
35_E39_E1.6031.00
49_D52_Q1.5741.00
25_E28_E1.5351.00
3_K40_K1.5271.00
6_V44_I1.4901.00
23_L55_F1.4771.00
1_L11_Y1.4751.00
21_S25_E1.4391.00
48_Y53_N1.3641.00
12_V26_L1.3611.00
38_E55_F1.2801.00
11_Y15_K1.2581.00
39_E43_A1.1341.00
6_V46_Y1.1301.00
33_D36_E1.1221.00
9_L46_Y1.1101.00
1_L32_I1.0861.00
23_L37_L1.0761.00
22_S25_E1.0701.00
11_Y30_L1.0541.00
7_M10_S1.0331.00
6_V9_L1.0221.00
35_E38_E0.9921.00
7_M14_T0.9861.00
16_L53_N0.9151.00
8_L41_L0.8981.00
39_E42_A0.8590.99
9_L44_I0.8540.99
36_E40_K0.8500.99
5_P40_K0.8390.99
19_E29_D0.8300.99
40_K43_A0.8230.99
20_Y30_L0.8180.99
23_L34_K0.7760.99
10_S18_D0.7740.99
5_P44_I0.7680.99
14_T18_D0.7630.99
32_I37_L0.7530.98
49_D54_Q0.7280.98
14_T19_E0.7080.98
19_E30_L0.7050.98
6_V54_Q0.7000.97
22_S53_N0.6880.97
7_M11_Y0.6470.96
11_Y26_L0.6270.95
27_C34_K0.6240.95
24_D50_E0.6170.95
2_P11_Y0.6120.95
2_P5_P0.6070.94
28_E31_D0.6010.94
14_T26_L0.5900.93
40_K44_I0.5860.93
39_E47_E0.5830.93
32_I40_K0.5770.93
15_K20_Y0.5730.92
47_E51_E0.5690.92
10_S20_Y0.5510.91
39_E50_E0.5500.91
36_E39_E0.5490.91
15_K29_D0.5440.90
22_S50_E0.5390.90
14_T52_Q0.5370.90
38_E42_A0.5340.90
3_K11_Y0.5330.90
19_E22_S0.5320.89
4_D45_G0.5230.89
6_V45_G0.5180.88
31_D45_G0.5120.88
20_Y48_Y0.5050.87
10_S15_K0.5030.87
7_M15_K0.5020.87
50_E53_N0.5000.86
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2fi0A 1 0.9455 53.1 0.827 Contact Map
2k9sA 1 0.9636 29.7 0.851 Contact Map
2p22B 2 0.4364 25.1 0.856 Contact Map
3lsgA 2 0.9818 23.8 0.858 Contact Map
2f6mB 1 0.8 23 0.859 Contact Map
1rr7A 2 0.8727 22.5 0.859 Contact Map
3mn2A 1 0.9636 20 0.862 Contact Map
3oioA 1 0.9818 19.7 0.863 Contact Map
1d5yA 1 0.9455 16.4 0.868 Contact Map
3rkxA 1 0.9091 15.6 0.869 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0046 seconds.