GREMLIN Database
DUF4249 - Domain of unknown function (DUF4249)
PFAM: PF14054 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 294 (267)
Sequences: 14727 (13581)
Seq/√Len: 831.2
META: 0.896

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
72_D97_K3.9311.00
240_K244_D3.6521.00
73_N97_K3.4351.00
69_T102_E3.1221.00
94_E97_K3.0791.00
71_S102_E2.9161.00
30_V103_I2.7751.00
100_T115_T2.7271.00
72_D76_N2.6741.00
67_T104_E2.6501.00
32_G43_V2.5691.00
71_S100_T2.5241.00
69_T77_T2.4901.00
26_P47_R2.4681.00
74_D97_K2.4571.00
98_T115_T2.4551.00
28_L63_V2.3511.00
104_E111_Y2.3421.00
234_K292_S2.3361.00
102_E111_Y2.2741.00
65_G82_E2.1961.00
28_L105_T2.1721.00
248_S275_Y2.1631.00
232_L292_S2.1411.00
98_T117_T2.1361.00
118_I287_A2.0661.00
64_S82_E2.0071.00
159_W235_L1.9921.00
45_L66_A1.9371.00
116_T239_S1.9211.00
116_T241_E1.9171.00
82_E85_N1.9161.00
129_Y146_N1.8741.00
236_Y290_V1.8621.00
116_T287_A1.8601.00
158_R236_Y1.8491.00
238_I242_Y1.8291.00
242_Y286_S1.7861.00
106_P109_G1.7781.00
278_I281_G1.7681.00
156_Y240_K1.7471.00
242_Y246_W1.7381.00
234_K290_V1.7241.00
93_G118_I1.7031.00
42_T87_Y1.6781.00
42_T89_T1.6601.00
126_D148_T1.6481.00
27_K282_L1.6331.00
72_D94_E1.5701.00
113_S279_E1.5611.00
73_N98_T1.5531.00
47_R63_V1.5301.00
100_T112_V1.5221.00
100_T113_S1.5071.00
63_V105_T1.4991.00
36_N289_S1.4981.00
44_K87_Y1.4981.00
69_T79_T1.4971.00
69_T104_E1.4851.00
102_E110_E1.4751.00
30_V45_L1.4741.00
102_E113_S1.4391.00
158_R246_W1.4391.00
72_D99_Y1.4341.00
47_R105_T1.4311.00
71_S77_T1.4311.00
236_Y288_S1.4301.00
239_S242_Y1.4281.00
63_V66_A1.4141.00
158_R238_I1.4081.00
31_E44_K1.3911.00
215_R218_G1.3851.00
83_T89_T1.3851.00
243_Y247_K1.3731.00
127_S146_N1.3641.00
194_F197_G1.3631.00
45_L68_V1.3531.00
197_G200_I1.3401.00
36_N118_I1.3261.00
45_L103_I1.3191.00
34_I41_Q1.3181.00
244_D247_K1.3151.00
104_E110_E1.3151.00
247_K252_Q1.3071.00
30_V278_I1.3021.00
123_P291_S1.2991.00
75_G78_Y1.2961.00
105_T108_G1.2761.00
155_N237_S1.2751.00
41_Q93_G1.2731.00
102_E112_V1.2671.00
126_D129_Y1.2611.00
87_Y90_T1.2571.00
189_G192_D1.2501.00
78_Y91_F1.2391.00
30_V101_L1.2351.00
193_E198_D1.2131.00
32_G285_F1.2101.00
33_L286_S1.2101.00
18_P21_P1.2001.00
30_V112_V1.1911.00
50_P53_D1.1791.00
111_Y114_A1.1791.00
70_I80_L1.1761.00
191_L194_F1.1561.00
66_A103_I1.1391.00
241_E284_Y1.1301.00
105_T109_G1.1241.00
28_L45_L1.1201.00
82_E86_G1.1131.00
149_D155_N1.1111.00
121_P289_S1.1091.00
95_P120_P1.1061.00
28_L112_V1.1051.00
194_F198_D1.1021.00
65_G105_T1.0971.00
6_L10_S1.0941.00
36_N121_P1.0921.00
27_K48_S1.0911.00
121_P291_S1.0881.00
112_V116_T1.0771.00
242_Y284_Y1.0741.00
67_T79_T1.0731.00
154_G240_K1.0651.00
204_Y207_G1.0551.00
150_P155_N1.0521.00
2_L6_L1.0411.00
41_Q90_T1.0391.00
188_N191_L1.0301.00
132_F135_G1.0301.00
133_E142_E1.0241.00
195_L200_I1.0211.00
241_E275_Y1.0201.00
41_Q80_L1.0191.00
63_V106_P1.0171.00
34_I116_T1.0161.00
68_V103_I1.0051.00
241_E244_D1.0011.00
65_G106_P0.9901.00
173_V176_L0.9891.00
22_E50_P0.9861.00
239_S286_S0.9851.00
244_D248_S0.9811.00
244_D275_Y0.9771.00
34_I99_Y0.9741.00
109_G279_E0.9611.00
193_E196_N0.9581.00
35_T288_S0.9551.00
169_Y172_N0.9521.00
5_L9_L0.9501.00
33_L288_S0.9491.00
24_T48_S0.9421.00
196_N199_R0.9411.00
168_T171_S0.9251.00
176_L179_T0.9231.00
191_L197_G0.9221.00
95_P121_P0.9211.00
4_L8_L0.9191.00
29_V48_S0.9151.00
43_V68_V0.9141.00
193_E197_G0.9121.00
147_F235_L0.9021.00
141_V145_V0.9001.00
190_F193_E0.9001.00
150_P153_E0.8981.00
198_D201_S0.8951.00
194_F199_R0.8951.00
278_I285_F0.8901.00
195_L198_D0.8881.00
162_I165_C0.8851.00
247_K250_K0.8821.00
122_P237_S0.8791.00
34_I287_A0.8771.00
246_W286_S0.8751.00
37_G292_S0.8751.00
109_G112_V0.8741.00
101_L285_F0.8731.00
33_L236_Y0.8681.00
36_N95_P0.8641.00
15_E18_P0.8601.00
188_N192_D0.8561.00
56_S59_P0.8561.00
204_Y208_F0.8561.00
114_A278_I0.8551.00
36_N93_G0.8391.00
6_L9_L0.8361.00
188_N193_E0.8361.00
83_T86_G0.8311.00
276_G282_L0.8291.00
39_G42_T0.8261.00
41_Q70_I0.8251.00
156_Y243_Y0.8181.00
250_K255_E0.8171.00
29_V282_L0.8141.00
32_G287_A0.8111.00
31_E284_Y0.8061.00
196_N200_I0.8021.00
41_Q78_Y0.7991.00
37_G40_P0.7981.00
248_S274_V0.7941.00
192_D198_D0.7931.00
135_G138_D0.7931.00
34_I93_G0.7901.00
192_D196_N0.7881.00
95_P118_I0.7871.00
158_R242_Y0.7851.00
59_P62_P0.7841.00
105_T110_E0.7841.00
70_I285_F0.7841.00
192_D197_G0.7811.00
238_I246_W0.7791.00
199_R202_N0.7791.00
114_A285_F0.7701.00
151_P155_N0.7691.00
48_S282_L0.7691.00
245_Y249_L0.7591.00
187_D190_F0.7581.00
41_Q91_F0.7551.00
37_G92_K0.7531.00
32_G41_Q0.7521.00
64_S84_G0.7481.00
171_S175_L0.7451.00
195_L199_R0.7401.00
5_L8_L0.7401.00
35_T290_V0.7371.00
216_F221_Y0.7341.00
214_E218_G0.7321.00
121_P237_S0.7261.00
215_R219_K0.7231.00
140_G208_F0.7201.00
66_A105_T0.7201.00
52_F56_S0.7181.00
40_P89_T0.7181.00
131_E142_E0.7181.00
256_N261_D0.7171.00
96_G120_P0.7161.00
254_T257_S0.7161.00
107_D110_E0.7131.00
28_L111_Y0.7131.00
64_S85_N0.7131.00
94_E99_Y0.7091.00
47_R109_G0.7081.00
7_L10_S0.7041.00
206_L209_I0.7011.00
162_I166_W0.6951.00
43_V47_R0.6951.00
122_P150_P0.6941.00
238_I243_Y0.6931.00
30_V281_G0.6931.00
163_Y166_W0.6921.00
225_L229_D0.6911.00
191_L200_I0.6911.00
74_D94_E0.6901.00
249_L274_V0.6901.00
77_T102_E0.6891.00
139_D145_V0.6871.00
95_P289_S0.6861.00
189_G193_E0.6821.00
52_F55_T0.6801.00
112_V281_G0.6791.00
131_E202_N0.6761.00
127_S130_Y0.6751.00
170_F174_I0.6721.00
33_L284_Y0.6711.00
142_E205_P0.6681.00
24_T50_P0.6681.00
18_P22_E0.6641.00
10_S13_E0.6621.00
271_P274_V0.6611.00
246_W284_Y0.6591.00
125_I128_I0.6581.00
36_N287_A0.6541.00
235_L291_S0.6541.00
253_Q257_S0.6481.00
29_V46_S0.6451.00
141_V147_F0.6441.00
191_L195_L0.6421.00
205_P208_F0.6421.00
35_T234_K0.6401.00
15_E19_D0.6391.00
33_L242_Y0.6381.00
218_G221_Y0.6341.00
93_G99_Y0.6331.00
35_T39_G0.6301.00
55_T58_P0.6301.00
80_L90_T0.6291.00
189_G194_F0.6271.00
158_R286_S0.6271.00
35_T41_Q0.6251.00
173_V177_G0.6241.00
14_K17_D0.6221.00
131_E134_E0.6191.00
118_I289_S0.6181.00
148_T151_P0.6171.00
145_V235_L0.6161.00
79_T82_E0.6161.00
226_K229_D0.6161.00
149_D157_Y0.6131.00
130_Y133_E0.6091.00
168_T172_N0.6091.00
27_K280_G0.6091.00
201_S205_P0.6081.00
189_G198_D0.6071.00
202_N205_P0.6071.00
20_L24_T0.6061.00
238_I286_S0.6041.00
26_P48_S0.6021.00
225_L228_G0.5961.00
198_D202_N0.5961.00
125_I235_L0.5931.00
169_Y174_I0.5901.00
96_G117_T0.5881.00
43_V80_L0.5881.00
108_G111_Y0.5871.00
78_Y90_T0.5861.00
52_F57_P0.5861.00
211_F216_F0.5851.00
103_I281_G0.5841.00
273_N280_G0.5831.00
3_L7_L0.5831.00
113_S117_T0.5801.00
207_G210_P0.5781.00
190_F194_F0.5781.00
31_E245_Y0.5771.00
160_E163_Y0.5771.00
110_E113_S0.5741.00
248_S273_N0.5741.00
191_L196_N0.5731.00
40_P92_K0.5731.00
73_N100_T0.5721.00
129_Y202_N0.5711.00
37_G290_V0.5701.00
126_D130_Y0.5701.00
191_L198_D0.5661.00
34_I91_F0.5631.00
221_Y229_D0.5631.00
20_L23_A0.5631.00
120_P153_E0.5621.00
142_E145_V0.5611.00
170_F173_V0.5611.00
246_W249_L0.5591.00
71_S75_G0.5561.00
193_E199_R0.5551.00
152_G217_N0.5541.00
167_D171_S0.5521.00
114_A241_E0.5511.00
205_P210_P0.5491.00
133_E136_D0.5481.00
75_G80_L0.5461.00
84_G87_Y0.5461.00
190_F198_D0.5451.00
81_T84_G0.5451.00
167_D170_F0.5441.00
55_T59_P0.5431.00
148_T199_R0.5421.00
72_D78_Y0.5411.00
210_P213_D0.5391.00
141_V144_Y0.5361.00
172_N176_L0.5351.00
127_S131_E0.5351.00
141_V146_N0.5331.00
132_F136_D0.5321.00
36_N41_Q0.5311.00
276_G280_G0.5311.00
187_D191_L0.5301.00
112_V115_T0.5291.00
212_D218_G0.5281.00
36_N92_K0.5281.00
38_P92_K0.5271.00
38_P41_Q0.5271.00
49_V53_D0.5261.00
259_L262_P0.5261.00
270_Q273_N0.5221.00
193_E200_I0.5221.00
151_P154_G0.5221.00
258_G261_D0.5211.00
134_E139_D0.5211.00
241_E277_N0.5191.00
140_G143_I0.5171.00
204_Y209_I0.5171.00
237_S289_S0.5161.00
245_Y274_V0.5161.00
192_D199_R0.5141.00
171_S174_I0.5131.00
93_G285_F0.5131.00
35_T236_Y0.5131.00
238_I288_S0.5121.00
165_C168_T0.5101.00
19_D22_E0.5101.00
31_E46_S0.5091.00
133_E137_D0.5071.00
189_G196_N0.5071.00
190_F195_L0.5061.00
270_Q274_V0.5041.00
257_S261_D0.5041.00
175_L179_T0.5031.00
211_F219_K0.5031.00
123_P150_P0.5021.00
111_Y116_T0.5001.00
211_F215_R0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1ti6B 2 0.4286 26.5 0.936 Contact Map
3zbiC 1 0.0918 15.9 0.942 Contact Map
4jg9A 2 0.4218 3.7 0.957 Contact Map
3e8vA 1 0.2653 2.9 0.959 Contact Map
1h8lA 1 0.3946 2.9 0.959 Contact Map
4kytB 2 0.051 2.8 0.959 Contact Map
3imaB 1 0.2891 2.6 0.96 Contact Map
1n7lA 1 0.0612 2.4 0.961 Contact Map
4n6tA 1 0.2687 2 0.963 Contact Map
4go6A 1 0.1395 1.7 0.964 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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