GREMLIN Database
DUF4248 - Domain of unknown function (DUF4248)
PFAM: PF14053 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 68 (67)
Sequences: 1477 (1130)
Seq/√Len: 138.1
META: 0.869

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
44_L60_L2.3871.00
7_K24_V2.1921.00
21_K25_R2.1761.00
32_K38_K2.1341.00
54_T57_Q1.9361.00
38_K42_E1.8481.00
44_L57_Q1.8331.00
15_F26_K1.8321.00
50_Q57_Q1.7451.00
36_E64_Y1.6841.00
6_G9_E1.6231.00
18_L26_K1.5991.00
34_N64_Y1.5941.00
15_F30_W1.5361.00
12_Q20_P1.4991.00
19_T22_S1.4701.00
40_E43_A1.4511.00
8_S12_Q1.4511.00
24_V28_R1.4501.00
39_E43_A1.4391.00
2_I5_Y1.3721.00
40_E63_E1.3171.00
26_K30_W1.2671.00
40_E60_L1.2521.00
13_L62_V1.2281.00
8_S51_R1.2171.00
15_F18_L1.2101.00
59_R63_E1.2031.00
28_R32_K1.1811.00
22_S26_K1.1811.00
10_L61_I1.1540.99
18_L22_S1.1540.99
4_S55_P1.1380.99
16_P23_A1.1260.99
35_P38_K1.1170.99
40_E64_Y1.1160.99
39_E42_E1.0670.99
8_S20_P1.0520.99
29_R33_R1.0350.99
31_I38_K1.0300.99
10_L58_V1.0190.99
24_V51_R1.0140.98
56_R59_R1.0110.98
55_P66_G0.9900.98
27_L31_I0.9870.98
7_K53_F0.9520.98
3_R9_E0.9440.98
46_Y53_F0.9320.97
36_E40_E0.9260.97
40_E44_L0.9010.97
56_R63_E0.8950.97
8_S24_V0.8820.96
37_L60_L0.8490.95
5_Y13_L0.8270.95
10_L14_Y0.8060.94
7_K46_Y0.8010.94
5_Y58_V0.7980.94
26_K52_T0.7800.93
41_L46_Y0.7730.93
47_R50_Q0.7710.93
62_V67_E0.7590.92
50_Q62_V0.7560.92
18_L33_R0.7490.91
26_K29_R0.7370.91
7_K28_R0.7300.90
20_P23_A0.7190.90
41_L45_G0.7030.89
17_D35_P0.6860.87
57_Q60_L0.6810.87
30_W67_E0.6770.87
9_E13_L0.6460.84
28_R53_F0.6430.84
14_Y68_P0.6370.83
37_L65_L0.6350.83
36_E39_E0.6040.80
35_P64_Y0.6010.80
34_N37_L0.5870.78
25_R28_R0.5840.78
5_Y59_R0.5800.77
46_Y66_G0.5760.77
15_F52_T0.5640.75
39_E63_E0.5620.75
60_L64_Y0.5610.75
32_K48_K0.5560.74
22_S25_R0.5470.73
31_I37_L0.5350.71
24_V53_F0.5270.70
25_R29_R0.5260.70
49_R57_Q0.5260.70
11_A28_R0.5250.70
4_S52_T0.5250.70
4_S54_T0.5200.69
29_R32_K0.5200.69
17_D38_K0.5180.69
45_G66_G0.5150.69
30_W34_N0.5100.68
60_L63_E0.5080.67
6_G11_A0.5060.67
7_K51_R0.5050.67
61_I64_Y0.5000.66
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1q06A 2 0.7941 32 0.857 Contact Map
2vz4A 2 0.8088 23.2 0.867 Contact Map
3whpA 1 0 23.1 0.867 Contact Map
1r8dA 2 0.8235 22.9 0.867 Contact Map
3qaoA 2 0.8382 21.9 0.868 Contact Map
4r4eB 2 0.8382 21.4 0.869 Contact Map
4r24B 2 0.8382 19.1 0.872 Contact Map
1r8eA 2 0.7794 19.1 0.872 Contact Map
2jmlA 1 0.8676 17.2 0.875 Contact Map
3gp4A 4 0.8088 15 0.878 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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