GREMLIN Database
DUF4246 - Protein of unknown function (DUF4246)
PFAM: PF14033 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 469 (461)
Sequences: 560 (434)
Seq/√Len: 20.2
META: 0.071

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
217_L441_L7.1321.00
428_E432_P6.8591.00
328_E428_E5.4551.00
221_A348_A4.9741.00
86_D412_R4.0401.00
94_R411_G3.7801.00
22_D25_E3.6971.00
133_L455_T3.5601.00
218_I443_L3.5411.00
184_D456_A3.5061.00
228_V415_A3.4991.00
14_M18_T3.4881.00
353_D437_H3.2891.00
302_L306_F3.2031.00
440_I444_F3.1891.00
101_V317_L3.1551.00
33_V53_V3.0190.99
96_R216_K2.9260.99
183_C195_I2.8820.99
20_K39_E2.8410.99
49_T52_M2.7090.98
355_E434_K2.6380.98
105_E439_K2.4390.97
319_N439_K2.4210.97
439_K444_F2.3700.96
193_V219_A2.3340.96
100_A213_V2.2950.96
424_V438_R2.2820.96
226_E301_D2.2710.95
127_H318_A2.1910.94
114_W408_T2.1880.94
91_D94_R2.1210.93
351_Y408_T2.1200.93
312_Q454_S2.0570.92
359_E407_E2.0550.92
351_Y424_V2.0540.92
114_W123_L2.0310.92
26_K115_H2.0300.92
119_D326_K2.0240.92
187_V193_V1.9800.91
89_V220_R1.9230.89
187_V310_G1.9160.89
129_S180_W1.8880.88
98_R353_D1.8810.88
127_H424_V1.8500.87
20_K438_R1.8490.87
332_G438_R1.8420.87
14_M63_K1.8420.87
93_L435_P1.8380.87
363_S425_S1.8370.87
217_L350_Y1.8320.87
109_E112_K1.8270.87
15_N19_D1.8210.86
127_H381_Q1.8180.86
318_A337_E1.8020.86
332_G351_Y1.7880.85
175_S178_F1.7850.85
318_A424_V1.7690.85
202_L211_Y1.7480.84
49_T281_E1.7320.84
121_Q330_P1.7260.83
332_G424_V1.7160.83
94_R410_E1.7140.83
349_L442_A1.7010.82
361_R405_S1.6880.82
185_V195_I1.6860.82
115_H122_V1.6850.82
147_G150_D1.6850.82
351_Y362_L1.6820.82
33_V61_R1.6810.82
38_A42_A1.6580.81
118_S329_Y1.6480.80
113_D424_V1.6470.80
318_A381_Q1.6440.80
358_T437_H1.6050.78
218_I225_W1.5650.77
363_S423_R1.5540.76
221_A347_T1.5450.76
56_C81_G1.5380.75
122_V416_F1.5350.75
113_D351_Y1.5320.75
175_S339_M1.5230.75
424_V442_A1.5210.75
108_P112_K1.5200.74
318_A332_G1.5110.74
127_H180_W1.5100.74
115_H127_H1.5010.74
421_Q438_R1.5010.74
37_R54_D1.5000.73
351_Y438_R1.4940.73
20_K127_H1.4920.73
127_H332_G1.4820.73
41_L50_E1.4760.72
35_K113_D1.4710.72
20_K26_K1.4710.72
26_K424_V1.4660.72
331_G426_P1.4610.71
116_P127_H1.4570.71
71_G97_L1.4430.70
422_H438_R1.4320.70
120_G326_K1.4240.69
453_I457_N1.4200.69
316_K332_G1.4040.68
215_E457_N1.3920.68
329_Y352_Y1.3910.68
381_Q438_R1.3900.68
20_K424_V1.3790.67
365_R421_Q1.3770.67
318_A444_F1.3770.67
30_E61_R1.3740.67
270_W458_V1.3730.67
337_E424_V1.3720.66
358_T427_F1.3710.66
225_W348_A1.3680.66
26_K127_H1.3680.66
63_K83_W1.3670.66
115_H332_G1.3600.66
113_D332_G1.3550.65
18_T63_K1.3540.65
26_K35_K1.3500.65
19_D84_K1.3500.65
334_W365_R1.3460.65
53_V56_C1.3430.65
26_K337_E1.3290.64
6_R10_M1.3290.64
127_H360_S1.3280.64
115_H318_A1.3270.64
258_E262_D1.3270.64
26_K351_Y1.3250.64
127_H438_R1.3090.63
122_V129_S1.3080.63
312_Q452_I1.3080.63
133_L138_T1.2910.62
33_V138_T1.2860.61
133_L140_V1.2850.61
113_D316_K1.2840.61
102_A328_E1.2780.61
76_L138_T1.2750.61
94_R350_Y1.2700.60
107_V112_K1.2660.60
360_S424_V1.2620.60
337_E351_Y1.2600.60
320_I329_Y1.2500.59
113_D116_P1.2430.59
115_H424_V1.2420.59
319_N352_Y1.2340.58
15_N84_K1.2320.58
20_K422_H1.2260.58
115_H444_F1.2230.57
343_H450_I1.2220.57
269_E273_N1.2210.57
332_G337_E1.2190.57
312_Q451_R1.2190.57
333_S424_V1.2170.57
206_K468_E1.2160.57
113_D438_R1.2130.57
181_L458_V1.2100.57
438_R442_A1.2100.57
11_M75_V1.2050.56
381_Q442_A1.2000.56
429_L436_G1.1930.55
20_K32_I1.1910.55
134_V138_T1.1910.55
27_V64_A1.1840.55
74_S226_E1.1820.55
332_G381_Q1.1790.55
20_K332_G1.1690.54
318_A442_A1.1680.54
195_I218_I1.1610.53
18_T84_K1.1600.53
352_Y439_K1.1570.53
39_E438_R1.1570.53
408_T424_V1.1530.53
337_E416_F1.1490.53
116_P444_F1.1450.52
20_K35_K1.1440.52
115_H381_Q1.1440.52
359_E383_D1.1440.52
35_K39_E1.1430.52
116_P362_L1.1420.52
55_Y391_Y1.1410.52
118_S335_H1.1410.52
24_W67_F1.1380.52
154_S157_K1.1360.52
258_E263_D1.1310.51
26_K318_A1.1240.51
116_P124_D1.1220.51
39_E422_H1.1210.51
306_F449_H1.1200.51
358_T430_A1.1100.50
143_D161_I1.1100.50
127_H351_Y1.1070.50
33_V240_L1.1070.50
225_W415_A1.1060.50
323_T360_S1.0960.49
411_G420_L1.0950.49
88_L413_L1.0920.49
329_Y427_F1.0910.49
317_L441_L1.0900.49
114_W193_V1.0880.49
454_S465_W1.0790.48
32_I35_K1.0790.48
13_L17_I1.0780.48
35_K318_A1.0780.48
104_L125_L1.0750.48
140_V453_I1.0730.48
13_L120_G1.0710.47
115_H316_K1.0700.47
39_E381_Q1.0680.47
39_E318_A1.0670.47
257_D263_D1.0620.47
124_D127_H1.0560.47
115_H351_Y1.0560.47
91_D95_S1.0510.46
329_Y440_I1.0500.46
347_T414_L1.0490.46
124_D444_F1.0480.46
387_L390_V1.0470.46
105_E319_N1.0420.46
102_A106_D1.0390.45
180_W344_I1.0310.45
362_L442_A1.0170.44
424_V440_I1.0140.44
60_L341_N1.0090.43
15_N18_T1.0050.43
39_E127_H0.9990.43
161_I164_P0.9970.43
225_W443_L0.9940.43
332_G362_L0.9880.42
122_V424_V0.9870.42
332_G427_F0.9840.42
202_L455_T0.9830.42
329_Y351_Y0.9790.42
11_M227_R0.9790.42
202_L466_W0.9750.41
316_K438_R0.9750.41
383_D386_G0.9720.41
427_F438_R0.9690.41
20_K318_A0.9680.41
116_P318_A0.9640.41
89_V94_R0.9630.41
123_L321_H0.9600.40
14_M56_C0.9600.40
428_E433_T0.9590.40
201_N439_K0.9580.40
332_G360_S0.9510.40
3_L226_E0.9500.40
10_M49_T0.9480.40
180_W316_K0.9480.40
184_D195_I0.9420.39
3_L8_L0.9420.39
318_A438_R0.9410.39
105_E108_P0.9400.39
362_L408_T0.9370.39
302_L311_L0.9360.39
302_L413_L0.9330.39
180_W318_A0.9320.39
122_V316_K0.9320.39
175_S341_N0.9290.38
408_T414_L0.9250.38
27_V61_R0.9190.38
122_V362_L0.9180.38
345_V456_A0.9170.38
118_S127_H0.9160.38
113_D122_V0.9110.37
224_L415_A0.9110.37
203_H465_W0.9110.37
24_W413_L0.9060.37
197_S211_Y0.9050.37
72_I224_L0.9040.37
30_E119_D0.9040.37
122_V351_Y0.9020.37
123_L127_H0.9000.37
15_N75_V0.8990.37
56_C114_W0.8940.36
86_D134_V0.8920.36
13_L48_L0.8900.36
215_E225_W0.8880.36
26_K116_P0.8880.36
116_P122_V0.8860.36
184_D312_Q0.8840.36
148_L195_I0.8820.36
280_P283_G0.8790.35
105_E444_F0.8750.35
315_V443_L0.8750.35
185_V420_L0.8730.35
333_S360_S0.8720.35
126_V175_S0.8720.35
27_V33_V0.8710.35
105_E352_Y0.8670.35
122_V127_H0.8660.35
138_T167_E0.8650.35
334_W416_F0.8650.35
20_K115_H0.8640.35
232_L336_V0.8630.34
231_D309_R0.8630.34
333_S381_Q0.8620.34
397_G414_L0.8610.34
130_L331_G0.8610.34
352_Y440_I0.8540.34
49_T62_D0.8510.34
318_A351_Y0.8510.34
319_N357_I0.8500.34
104_L202_L0.8470.34
5_L8_L0.8450.33
379_Y386_G0.8440.33
129_S332_G0.8340.33
10_M14_M0.8330.33
131_F432_P0.8310.33
453_I458_V0.8310.33
126_V202_L0.8250.32
115_H440_I0.8230.32
381_Q416_F0.8220.32
4_T8_L0.8220.32
113_D318_A0.8210.32
319_N444_F0.8160.32
106_D140_V0.8090.31
131_F452_I0.8080.31
332_G422_H0.8060.31
26_K314_I0.8010.31
463_P467_A0.8010.31
211_Y215_E0.8010.31
183_C197_S0.7990.31
323_T427_F0.7980.31
446_V454_S0.7970.31
116_P381_Q0.7950.31
127_H422_H0.7940.31
362_L381_Q0.7940.31
59_E63_K0.7930.30
28_F61_R0.7920.30
115_H416_F0.7920.30
48_L81_G0.7910.30
57_L178_F0.7910.30
204_P446_V0.7880.30
332_G444_F0.7870.30
35_K332_G0.7860.30
427_F436_G0.7850.30
386_G390_V0.7840.30
184_D454_S0.7830.30
48_L391_Y0.7830.30
115_H335_H0.7830.30
222_I226_E0.7810.30
179_Q347_T0.7800.30
325_E349_L0.7780.30
77_D80_S0.7780.30
56_C61_R0.7760.30
451_R454_S0.7750.29
337_E444_F0.7720.29
187_V226_E0.7720.29
308_G355_E0.7720.29
113_D314_I0.7710.29
300_V305_D0.7700.29
135_Y341_N0.7690.29
151_C156_G0.7670.29
32_I351_Y0.7670.29
225_W368_V0.7660.29
39_E130_L0.7650.29
141_L195_I0.7630.29
151_C158_G0.7620.29
356_N436_G0.7620.29
122_V332_G0.7610.29
132_P348_A0.7610.29
218_I315_V0.7600.29
17_I199_I0.7590.29
318_A360_S0.7570.29
332_G335_H0.7560.28
424_V444_F0.7540.28
122_V326_K0.7500.28
63_K67_F0.7490.28
383_D455_T0.7480.28
249_D254_P0.7460.28
228_V383_D0.7460.28
57_L224_L0.7460.28
321_H439_K0.7430.28
183_C443_L0.7410.28
118_S416_F0.7410.28
180_W418_N0.7380.28
221_A413_L0.7380.28
57_L283_G0.7380.28
335_H424_V0.7380.28
49_T61_R0.7360.27
6_R139_R0.7350.27
124_D129_S0.7330.27
48_L56_C0.7330.27
35_K434_K0.7330.27
416_F424_V0.7330.27
52_M105_E0.7320.27
127_H337_E0.7310.27
133_L202_L0.7310.27
18_T56_C0.7290.27
416_F444_F0.7280.27
184_D425_S0.7270.27
360_S438_R0.7260.27
26_K332_G0.7250.27
388_E394_E0.7250.27
127_H442_A0.7240.27
197_S202_L0.7190.27
318_A440_I0.7180.26
427_F437_H0.7180.26
180_W362_L0.7160.26
20_K316_K0.7160.26
187_V307_R0.7150.26
85_S454_S0.7140.26
7_E10_M0.7130.26
351_Y360_S0.7110.26
194_K215_E0.7070.26
146_V217_L0.7050.26
228_V386_G0.7040.26
35_K381_Q0.7030.26
76_L187_V0.7020.26
248_E255_P0.7000.26
204_P465_W0.6990.26
290_E430_A0.6990.26
353_D358_T0.6980.25
436_G448_P0.6980.25
208_R212_S0.6980.25
129_S329_Y0.6970.25
333_S421_Q0.6950.25
115_H337_E0.6950.25
58_A306_F0.6940.25
225_W310_G0.6930.25
118_S314_I0.6930.25
252_Y255_P0.6920.25
116_P335_H0.6920.25
426_P432_P0.6920.25
249_D252_Y0.6870.25
351_Y444_F0.6870.25
113_D333_S0.6870.25
222_I229_L0.6860.25
318_A416_F0.6860.25
357_I444_F0.6860.25
406_V414_L0.6860.25
124_D318_A0.6850.25
177_K355_E0.6840.25
224_L348_A0.6840.25
35_K422_H0.6830.25
381_Q424_V0.6820.25
187_V311_L0.6820.25
264_D273_N0.6820.25
350_Y364_F0.6810.25
16_A138_T0.6800.25
41_L53_V0.6790.25
40_A235_R0.6790.25
311_L449_H0.6790.25
48_L53_V0.6780.24
73_V84_K0.6770.24
337_E365_R0.6770.24
85_S179_Q0.6770.24
112_K123_L0.6760.24
132_P400_N0.6750.24
431_D458_V0.6740.24
20_K337_E0.6710.24
73_V77_D0.6710.24
258_E261_E0.6700.24
408_T415_A0.6690.24
75_V195_I0.6680.24
348_A456_A0.6680.24
362_L438_R0.6680.24
113_D130_L0.6670.24
226_E300_V0.6670.24
20_K349_L0.6660.24
126_V315_V0.6660.24
19_D63_K0.6650.24
11_M185_V0.6610.24
218_I221_A0.6610.24
21_P112_K0.6600.24
62_D285_F0.6580.24
362_L416_F0.6580.24
225_W406_V0.6580.24
317_L439_K0.6570.23
125_L202_L0.6560.23
381_Q422_H0.6550.23
113_D362_L0.6550.23
107_V329_Y0.6540.23
115_H362_L0.6520.23
401_Q445_L0.6520.23
135_Y241_R0.6520.23
76_L132_P0.6490.23
332_G416_F0.6490.23
255_P259_D0.6480.23
16_A53_V0.6480.23
124_D180_W0.6480.23
359_E458_V0.6470.23
259_D268_E0.6460.23
316_K427_F0.6430.23
115_H180_W0.6430.23
59_E241_R0.6430.23
343_H391_Y0.6400.23
248_E251_E0.6390.23
56_C63_K0.6380.23
132_P221_A0.6370.23
108_P114_W0.6370.23
16_A452_I0.6360.23
34_A326_K0.6350.22
33_V57_L0.6350.22
254_P257_D0.6350.22
124_D335_H0.6350.22
26_K129_S0.6290.22
53_V63_K0.6280.22
408_T412_R0.6260.22
127_H316_K0.6250.22
318_A362_L0.6250.22
14_M57_L0.6240.22
220_R224_L0.6240.22
246_D249_D0.6230.22
73_V185_V0.6220.22
360_S381_Q0.6220.22
7_E179_Q0.6210.22
152_L205_E0.6210.22
39_E424_V0.6210.22
35_K316_K0.6190.22
118_S122_V0.6160.22
332_G440_I0.6150.22
26_K122_V0.6150.22
174_F187_V0.6140.22
147_G190_D0.6130.21
20_K129_S0.6120.21
343_H414_L0.6120.21
312_Q401_Q0.6110.21
113_D127_H0.6100.21
138_T294_Y0.6090.21
24_W68_E0.6090.21
222_I310_G0.6090.21
448_P461_Q0.6080.21
217_L221_A0.6050.21
51_A54_D0.6050.21
254_P268_E0.6040.21
256_E259_D0.6040.21
74_S222_I0.6030.21
455_T461_Q0.6020.21
146_V152_L0.6000.21
115_H313_V0.6000.21
5_L9_A0.5970.21
318_A408_T0.5970.21
323_T327_P0.5950.21
199_I352_Y0.5940.21
111_E405_S0.5930.21
139_R143_D0.5930.21
218_I415_A0.5930.21
116_P442_A0.5920.21
75_V183_C0.5920.21
68_E214_I0.5890.20
248_E253_E0.5880.20
96_R325_E0.5860.20
118_S124_D0.5860.20
320_I326_K0.5850.20
177_K383_D0.5840.20
124_D357_I0.5840.20
362_L422_H0.5830.20
331_G337_E0.5830.20
129_S320_I0.5820.20
317_L440_I0.5820.20
108_P439_K0.5820.20
116_P416_F0.5820.20
334_W422_H0.5810.20
325_E384_D0.5800.20
115_H344_I0.5770.20
211_Y346_A0.5770.20
273_N276_P0.5770.20
18_T225_W0.5760.20
351_Y440_I0.5760.20
103_P213_V0.5750.20
191_G432_P0.5750.20
269_E299_P0.5740.20
115_H124_D0.5730.20
306_F309_R0.5730.20
26_K381_Q0.5700.20
229_L392_G0.5700.20
56_C194_K0.5690.20
359_E430_A0.5690.20
264_D391_Y0.5680.20
196_T310_G0.5680.20
216_K219_A0.5660.20
166_D169_T0.5650.19
90_P93_L0.5650.19
92_E328_E0.5640.19
422_H444_F0.5630.19
26_K113_D0.5630.19
134_V137_R0.5600.19
386_G460_P0.5590.19
121_Q222_I0.5590.19
346_A465_W0.5580.19
134_V168_E0.5570.19
342_E352_Y0.5570.19
286_F289_P0.5560.19
337_E408_T0.5560.19
10_M18_T0.5560.19
322_L332_G0.5560.19
270_W274_R0.5550.19
284_E462_Q0.5550.19
124_D438_R0.5540.19
351_Y442_A0.5530.19
107_V439_K0.5510.19
360_S427_F0.5500.19
196_T343_H0.5500.19
58_A62_D0.5500.19
184_D196_T0.5490.19
403_L419_I0.5490.19
26_K180_W0.5490.19
109_E222_I0.5470.19
30_E93_L0.5470.19
107_V130_L0.5470.19
255_P258_E0.5460.19
122_V381_Q0.5440.19
221_A225_W0.5440.19
186_E222_I0.5440.19
362_L424_V0.5430.19
24_W28_F0.5430.19
314_I342_E0.5430.19
114_W415_A0.5420.19
32_I381_Q0.5400.18
214_I315_V0.5400.18
113_D381_Q0.5370.18
4_T7_E0.5360.18
22_D309_R0.5350.18
63_K453_I0.5330.18
128_P180_W0.5330.18
121_Q331_G0.5330.18
137_R443_L0.5330.18
63_K75_V0.5330.18
339_M386_G0.5320.18
132_P178_F0.5320.18
116_P438_R0.5320.18
219_A224_L0.5320.18
96_R209_E0.5320.18
82_V410_E0.5310.18
53_V57_L0.5310.18
246_D251_E0.5310.18
89_V217_L0.5300.18
35_K351_Y0.5290.18
27_V82_V0.5280.18
210_L331_G0.5280.18
452_I457_N0.5270.18
172_Y385_E0.5270.18
180_W351_Y0.5270.18
165_P168_E0.5260.18
192_G432_P0.5250.18
256_E260_D0.5240.18
55_Y343_H0.5240.18
333_S427_F0.5240.18
329_Y333_S0.5240.18
255_P260_D0.5240.18
26_K438_R0.5230.18
82_V414_L0.5230.18
362_L444_F0.5230.18
226_E343_H0.5230.18
148_L423_R0.5210.18
113_D337_E0.5200.18
349_L424_V0.5190.18
140_V458_V0.5190.18
37_R53_V0.5180.18
129_S316_K0.5180.18
304_E313_V0.5180.18
139_R158_G0.5160.18
191_G413_L0.5160.18
394_E397_G0.5150.17
17_I414_L0.5150.17
124_D362_L0.5150.17
256_E261_E0.5150.17
306_F310_G0.5140.17
131_F344_I0.5140.17
105_E213_V0.5130.17
449_H452_I0.5130.17
122_V180_W0.5120.17
139_R156_G0.5120.17
112_K439_K0.5120.17
226_E303_R0.5120.17
224_L343_H0.5110.17
200_N344_I0.5110.17
101_V107_V0.5100.17
234_L238_R0.5100.17
95_S99_A0.5100.17
424_V427_F0.5100.17
137_R442_A0.5100.17
202_L214_I0.5100.17
76_L178_F0.5100.17
243_E275_R0.5100.17
54_D433_T0.5080.17
123_L317_L0.5070.17
134_V242_I0.5060.17
32_I320_I0.5060.17
143_D187_V0.5060.17
28_F63_K0.5050.17
32_I329_Y0.5030.17
222_I398_P0.5020.17
116_P424_V0.5020.17
335_H444_F0.5020.17
7_E11_M0.5010.17
325_E458_V0.5010.17
116_P337_E0.5010.17
292_P295_D0.5010.17
353_D359_E0.5000.17
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2rg4A 4 0.4392 12.6 0.984 Contact Map
3itqA 2 0.3518 11.2 0.984 Contact Map
4ajyV 1 0.1151 9.7 0.985 Contact Map
4wxxA 1 0.1279 8.4 0.985 Contact Map
3hzsA 3 0.1237 7.2 0.986 Contact Map
3vmtA 1 0.1258 6.3 0.986 Contact Map
4xlgB 1 0.1386 6 0.986 Contact Map
4wqoA 1 0.1151 5.8 0.986 Contact Map
3g7uA 1 0.113 5.7 0.986 Contact Map
3i5pA 1 0.1684 5.4 0.987 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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