GREMLIN Database
DUF4245 - Protein of unknown function (DUF4245)
PFAM: PF14030 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 166 (164)
Sequences: 2426 (1857)
Seq/√Len: 145.0
META: 0.666

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
83_H97_E4.4081.00
42_Y86_Y4.1021.00
7_R11_L3.6621.00
94_V162_L3.6021.00
92_Q153_T2.7231.00
70_S85_G2.6551.00
121_T126_T2.5881.00
39_T67_R2.4481.00
120_V160_R2.4031.00
160_R164_A2.2931.00
158_E161_T2.1901.00
8_D12_S2.0751.00
8_D11_L2.0531.00
111_L138_A2.0461.00
46_L82_W2.0441.00
67_R91_G1.9941.00
90_D158_E1.8611.00
120_V129_V1.7481.00
73_F80_P1.6871.00
156_D160_R1.6741.00
121_T124_G1.6721.00
66_W162_L1.6361.00
45_A71_A1.5931.00
17_L20_V1.5831.00
25_L29_R1.5821.00
125_R141_R1.5741.00
88_T158_E1.5601.00
119_T126_T1.5421.00
132_E135_G1.5391.00
11_L14_A1.5301.00
157_A160_R1.5241.00
21_L25_L1.5141.00
137_T156_D1.4801.00
61_G89_P1.4761.00
154_A158_E1.4361.00
150_V159_L1.4311.00
2_G8_D1.4291.00
6_V10_V1.3951.00
25_L28_P1.3921.00
142_T147_T1.3811.00
30_P34_P1.3711.00
18_V22_V1.3491.00
46_L84_V1.3441.00
62_L89_P1.3281.00
7_R12_S1.3261.00
26_F29_R1.3231.00
20_V29_R1.2981.00
11_L15_V1.2811.00
63_P161_T1.2771.00
53_A57_V1.2741.00
116_P128_Q1.2721.00
23_L27_V1.2711.00
42_Y68_A1.2531.00
43_A59_A1.2511.00
83_H93_Y1.2431.00
117_T129_V1.2281.00
62_L86_Y1.2191.00
43_A47_A1.2171.00
129_V156_D1.2151.00
135_G138_A1.2051.00
155_S158_E1.2031.00
150_V163_A1.2021.00
82_W98_Q1.2001.00
132_E136_E1.1991.00
79_V100_D1.1761.00
133_G136_E1.1671.00
152_G159_L1.1581.00
46_L59_A1.1571.00
111_L114_A1.1280.99
85_G93_Y1.1230.99
68_A86_Y1.1210.99
98_Q146_V1.1040.99
161_T165_A1.0970.99
58_L148_V1.0910.99
106_L111_L1.0900.99
95_G151_T1.0900.99
122_V125_R1.0790.99
42_Y82_W1.0720.99
18_V25_L1.0670.99
4_Q8_D1.0590.99
68_A71_A1.0540.99
45_A48_A1.0470.99
8_D16_L1.0470.99
42_Y84_V1.0430.99
12_S16_L1.0430.99
101_A106_L1.0390.99
23_L26_F1.0210.99
99_S106_L1.0150.99
54_G100_D1.0060.99
70_S83_H1.0030.99
17_L21_L0.9880.98
61_G65_G0.9850.98
2_G6_V0.9820.98
24_V27_V0.9730.98
134_G138_A0.9700.98
18_V21_L0.9640.98
83_H95_G0.9630.98
22_V26_F0.9410.98
24_V28_P0.9360.98
87_L93_Y0.9330.98
8_D19_V0.9320.98
69_T85_G0.9250.98
116_P130_Y0.9250.98
110_V149_V0.9230.98
120_V156_D0.9180.98
37_V67_R0.9090.97
163_A166_L0.8930.97
17_L22_V0.8920.97
55_F100_D0.8860.97
89_P158_E0.8600.96
60_P84_V0.8470.96
139_L163_A0.8430.96
28_P106_L0.8380.96
3_R12_S0.8350.96
46_L50_A0.8330.96
122_V131_T0.8220.95
81_T97_E0.8170.95
69_T93_Y0.8150.95
3_R6_V0.8140.95
16_L19_V0.8110.95
21_L24_V0.8030.95
106_L110_V0.8030.95
5_T8_D0.8030.95
40_V71_A0.7900.94
94_V129_V0.7900.94
121_T160_R0.7890.94
133_G139_L0.7880.94
69_T87_L0.7840.94
86_Y96_L0.7840.94
132_E137_T0.7830.94
135_G141_R0.7730.94
33_D37_V0.7700.93
75_G78_G0.7700.93
5_T20_V0.7680.93
43_A86_Y0.7660.93
162_L165_A0.7650.93
79_V106_L0.7580.93
156_D159_L0.7510.93
88_T94_V0.7450.92
143_A148_V0.7450.92
8_D17_L0.7330.92
131_T135_G0.7330.92
131_T134_G0.7300.91
27_V30_P0.7280.91
131_T139_L0.7270.91
94_V159_L0.7240.91
50_A59_A0.7230.91
88_T91_G0.7220.91
55_F80_P0.7180.91
135_G139_L0.7160.91
137_T159_L0.7150.91
39_T65_G0.7010.90
131_T137_T0.6950.89
104_A108_A0.6920.89
8_D27_V0.6910.89
34_P63_P0.6900.89
115_A118_G0.6840.89
127_W150_V0.6840.89
138_A141_R0.6840.89
112_G135_G0.6830.89
67_R87_L0.6790.88
61_G64_E0.6780.88
62_L68_A0.6730.88
4_Q7_R0.6700.88
17_L24_V0.6650.87
48_A52_A0.6640.87
20_V26_F0.6610.87
117_T156_D0.6600.87
51_R57_V0.6580.87
141_R144_G0.6540.86
120_V124_G0.6520.86
41_D44_A0.6510.86
24_V29_R0.6510.86
37_V70_S0.6480.86
59_A86_Y0.6420.85
94_V150_V0.6400.85
20_V27_V0.6390.85
55_F146_V0.6390.85
2_G5_T0.6380.85
14_A18_V0.6350.85
132_E139_L0.6290.84
9_M16_L0.6290.84
53_A73_F0.6250.84
37_V87_L0.6250.84
12_S15_V0.6230.84
119_T122_V0.6220.83
13_L20_V0.6190.83
143_A146_V0.6180.83
74_D81_T0.6180.83
20_V24_V0.6140.83
8_D101_A0.6100.82
68_A84_V0.6090.82
53_A80_P0.6040.82
16_L28_P0.6000.81
134_G137_T0.5980.81
3_R7_R0.5970.81
46_L98_Q0.5950.81
96_L162_L0.5860.80
65_G88_T0.5860.80
27_V33_D0.5850.80
5_T9_M0.5820.79
96_L150_V0.5820.79
121_T125_R0.5790.79
75_G80_P0.5790.79
159_L163_A0.5770.79
7_R13_L0.5750.78
133_G153_T0.5750.78
136_E139_L0.5710.78
73_F79_V0.5700.78
29_R32_F0.5680.78
44_A48_A0.5670.77
52_A73_F0.5660.77
119_T128_Q0.5630.77
4_Q12_S0.5620.77
49_A71_A0.5620.77
62_L65_G0.5610.77
15_V24_V0.5560.76
99_S102_A0.5540.76
103_A128_Q0.5540.76
132_E150_V0.5530.76
30_P33_D0.5490.75
74_D113_G0.5480.75
27_V31_S0.5480.75
54_G75_G0.5480.75
119_T129_V0.5450.75
49_A82_W0.5450.75
15_V19_V0.5440.74
50_A54_G0.5400.74
158_E162_L0.5390.74
101_A114_A0.5370.74
25_L30_P0.5350.73
131_T136_E0.5350.73
23_L33_D0.5340.73
121_T161_T0.5320.73
45_A91_G0.5260.72
70_S84_V0.5230.72
67_R141_R0.5200.71
6_V17_L0.5150.70
86_Y163_A0.5140.70
133_G150_V0.5110.70
142_T145_G0.5080.69
3_R9_M0.5040.69
114_A120_V0.5040.69
79_V101_A0.5030.69
146_V150_V0.5020.69
83_H110_V0.5000.68
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3lydA 2 0.747 33.6 0.921 Contact Map
4ps6A 1 0.5361 13 0.935 Contact Map
2ls2A 1 0.1506 7.5 0.942 Contact Map
3m4wA 2 0.7831 6.3 0.944 Contact Map
3vxvA 1 0.3855 5.7 0.945 Contact Map
2llzA 1 0.4518 4.7 0.947 Contact Map
3cp0A 1 0.3434 4.7 0.947 Contact Map
3wu2X 1 0.2229 4.2 0.948 Contact Map
3bz1X 1 0.2169 3.4 0.95 Contact Map
3q2bA 1 0.3012 3.1 0.952 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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