GREMLIN Database
DUF4243 - Protein of unknown function (DUF4243)
PFAM: PF14027 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 320 (302)
Sequences: 1843 (1531)
Seq/√Len: 88.1
META: 0.49

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
110_S113_E4.2001.00
110_S114_V3.9411.00
111_P114_V3.5661.00
69_R77_E3.5101.00
26_Y30_L3.3961.00
105_A117_A3.3101.00
42_E65_E3.2481.00
41_T44_N3.1311.00
177_L180_R2.7851.00
54_R57_D2.7491.00
33_Q38_P2.6521.00
174_R177_L2.6281.00
235_V315_L2.5281.00
150_A154_L2.5211.00
105_A114_V2.4431.00
25_A29_R2.1101.00
23_Q27_R2.0801.00
154_L157_V2.0531.00
55_Y59_L2.0111.00
42_E61_F2.0071.00
11_A104_H1.9891.00
70_G74_V1.9861.00
33_Q37_P1.9691.00
35_P38_P1.9251.00
109_G114_V1.9091.00
264_W268_A1.8951.00
188_Y191_A1.8531.00
311_H314_K1.8441.00
149_S152_E1.8421.00
97_H233_H1.8361.00
189_A192_W1.8261.00
61_F65_E1.8191.00
185_F188_Y1.8111.00
65_E68_E1.8001.00
27_R30_L1.7921.00
18_S21_V1.7901.00
4_H233_H1.7821.00
174_R178_L1.7811.00
151_L154_L1.7781.00
296_D299_E1.7771.00
62_F122_A1.7691.00
61_F78_W1.7681.00
182_G186_L1.7451.00
109_G113_E1.7381.00
76_A249_P1.7311.00
262_R266_Q1.7041.00
97_H311_H1.6941.00
233_H311_H1.6851.00
156_R160_D1.6801.00
21_V24_R1.6491.00
100_I233_H1.6121.00
241_L264_W1.5921.00
90_G93_A1.5781.00
183_P192_W1.5731.00
87_L90_G1.5611.00
239_H318_A1.5581.00
147_S150_A1.5571.00
173_I178_L1.5561.00
185_F192_W1.5551.00
96_T237_S1.5541.00
7_M101_R1.5321.00
59_L63_E1.5321.00
59_L116_E1.5281.00
175_D178_L1.5151.00
307_H312_V1.5141.00
126_Q129_P1.5141.00
173_I177_L1.5111.00
153_L156_R1.5061.00
69_R74_V1.4951.00
127_P130_G1.4841.00
10_E108_A1.4630.99
33_Q36_A1.4620.99
48_A54_R1.4610.99
100_I314_K1.4600.99
97_H314_K1.4500.99
188_Y194_V1.4300.99
102_L117_A1.3990.99
261_L265_W1.3890.99
106_V244_L1.3690.99
263_A266_Q1.3690.99
40_I44_N1.3640.99
188_Y192_W1.3620.99
34_R37_P1.3550.99
107_E317_R1.3530.99
184_E192_W1.3530.99
155_A159_A1.3520.99
104_H239_H1.3510.99
109_G112_A1.3500.99
33_Q39_A1.3330.99
58_W122_A1.3280.99
182_G185_F1.3230.99
34_R38_P1.3140.99
186_L189_A1.3070.99
4_H97_H1.2820.99
120_Y124_R1.2770.98
231_L275_R1.2740.98
204_K207_E1.2700.98
151_L155_A1.2660.98
91_L99_L1.2640.98
237_S264_W1.2570.98
148_T151_L1.2550.98
264_W272_Y1.2530.98
66_L70_G1.2520.98
15_L301_I1.2430.98
213_A319_C1.2350.98
174_R180_R1.2340.98
150_A265_W1.2310.98
156_R161_P1.2270.98
65_E69_R1.2230.98
233_H314_K1.2200.98
100_I240_A1.2150.98
5_L9_L1.2100.98
12_L313_I1.1990.98
30_L33_Q1.1940.98
100_I103_G1.1900.98
4_H311_H1.1690.97
271_L274_A1.1660.97
183_P188_Y1.1530.97
186_L190_A1.1370.97
149_S153_L1.1330.97
188_Y193_D1.1280.97
261_L264_W1.1260.97
149_S156_R1.1190.97
161_P165_G1.1130.96
184_E187_E1.1030.96
102_L114_V1.1000.96
9_L22_L1.0970.96
75_L106_V1.0940.96
97_H236_T1.0930.96
34_R39_A1.0920.96
157_V160_D1.0830.96
186_L191_A1.0790.96
182_G188_Y1.0750.96
185_F190_A1.0690.95
160_D188_Y1.0690.95
152_E155_A1.0570.95
4_H7_M1.0570.95
66_L74_V1.0560.95
232_L240_A1.0530.95
264_W274_A1.0510.95
150_A153_L1.0480.95
265_W272_Y1.0480.95
96_T267_A1.0450.95
45_W58_W1.0430.95
191_A194_V1.0410.95
236_T314_K1.0390.95
55_Y119_A1.0380.95
148_T152_E1.0330.95
49_L58_W1.0290.94
188_Y195_S1.0170.94
271_L275_R1.0160.94
269_A273_A1.0080.94
17_A302_A1.0060.94
233_H236_T1.0040.94
261_L267_A1.0030.94
176_G179_A1.0030.94
148_T156_R1.0020.94
104_H318_A0.9970.93
58_W78_W0.9950.93
185_F191_A0.9930.93
183_P193_D0.9920.93
209_A239_H0.9900.93
162_R166_A0.9890.93
183_P186_L0.9810.93
239_H243_L0.9780.93
40_I61_F0.9770.93
2_H95_A0.9680.92
75_L102_L0.9580.92
172_K178_L0.9480.92
149_S154_L0.9480.92
158_A272_Y0.9420.91
176_G180_R0.9380.91
265_W273_A0.9370.91
177_L181_A0.9370.91
162_R184_E0.9350.91
259_R262_R0.9350.91
192_W195_S0.9300.91
159_A162_R0.9300.91
232_L236_T0.9270.91
87_L122_A0.9250.91
4_H314_K0.9220.90
183_P190_A0.9170.90
87_L121_W0.9160.90
192_W196_P0.9150.90
155_A161_P0.9140.90
8_A25_A0.9130.90
241_L260_L0.9130.90
10_E104_H0.9130.90
146_G153_L0.9110.90
187_E191_A0.9070.90
270_A274_A0.9070.90
71_W114_V0.9060.90
185_F193_D0.9040.90
212_A215_L0.9030.90
153_L157_V0.9020.89
105_A108_A0.9010.89
46_R86_R0.8960.89
172_K177_L0.8960.89
48_A51_D0.8940.89
21_V305_V0.8930.89
2_H103_G0.8890.89
265_W275_R0.8780.88
264_W267_A0.8740.88
172_K175_D0.8700.88
38_P73_A0.8690.88
226_G230_F0.8630.87
178_L192_W0.8560.87
12_L25_A0.8540.87
10_E13_Y0.8510.87
102_L121_W0.8490.86
153_L159_A0.8490.86
45_W245_L0.8410.86
74_V115_A0.8400.86
178_L181_A0.8340.85
144_P147_S0.8330.85
172_K176_G0.8320.85
313_I316_V0.8320.85
25_A30_L0.8300.85
307_H310_E0.8280.85
260_L264_W0.8230.85
234_A268_A0.8220.85
268_A274_A0.8210.85
173_I180_R0.8200.84
75_L87_L0.8200.84
183_P191_A0.8190.84
63_E115_A0.8150.84
142_A146_G0.8140.84
300_L316_V0.8120.84
132_F135_S0.8110.84
10_E318_A0.8050.83
94_G110_S0.8040.83
97_H229_F0.8030.83
97_H100_I0.8030.83
151_L159_A0.8020.83
190_A193_D0.8020.83
173_I176_G0.7960.83
48_A58_W0.7960.83
52_R124_R0.7950.83
190_A194_V0.7930.82
101_R116_E0.7920.82
236_T311_H0.7870.82
187_E192_W0.7870.82
42_E77_E0.7850.82
96_T230_F0.7820.82
178_L182_G0.7770.81
161_P166_A0.7770.81
272_Y275_R0.7750.81
65_E78_W0.7720.81
163_L166_A0.7720.81
45_W242_R0.7710.81
4_H100_I0.7700.81
205_L245_L0.7690.80
230_F274_A0.7680.80
264_W271_L0.7640.80
17_A21_V0.7630.80
186_L193_D0.7600.80
262_R271_L0.7600.80
103_G243_L0.7580.80
265_W271_L0.7580.80
270_A275_R0.7570.79
151_L157_V0.7570.79
147_S154_L0.7560.79
302_A306_A0.7550.79
170_G173_I0.7550.79
272_Y276_G0.7550.79
217_L319_C0.7540.79
52_R123_A0.7510.79
14_A108_A0.7500.79
75_L114_V0.7450.78
230_F275_R0.7450.78
171_N180_R0.7440.78
26_Y29_R0.7400.78
160_D163_L0.7400.78
264_W275_R0.7330.77
150_A157_V0.7330.77
36_A39_A0.7310.77
158_A161_P0.7300.77
301_I305_V0.7220.76
156_R191_A0.7180.76
69_R73_A0.7170.76
267_A270_A0.7160.76
215_L234_A0.7110.75
154_L163_L0.7100.75
133_L136_A0.7040.74
101_R113_E0.7020.74
316_V319_C0.7020.74
275_R278_P0.7010.74
268_A271_L0.7000.74
150_A158_A0.6950.73
207_E210_E0.6920.73
101_R121_W0.6900.73
26_Y32_Y0.6900.73
10_E107_E0.6900.73
230_F317_R0.6870.73
149_S207_E0.6860.72
44_N47_E0.6860.72
209_A213_A0.6840.72
155_A162_R0.6840.72
102_L244_L0.6810.72
95_A230_F0.6810.72
46_R130_G0.6800.72
151_L160_D0.6790.72
161_P164_A0.6760.71
40_I49_L0.6740.71
162_R167_R0.6730.71
71_W109_G0.6730.71
154_L160_D0.6710.71
150_A155_A0.6710.71
268_A273_A0.6700.71
145_P154_L0.6660.70
208_L216_Y0.6630.70
184_E188_Y0.6620.70
261_L268_A0.6620.70
107_E228_D0.6620.70
299_E302_A0.6600.70
32_Y37_P0.6600.70
239_H317_R0.6600.70
175_D180_R0.6580.69
175_D179_A0.6580.69
29_R32_Y0.6580.69
235_V238_P0.6580.69
9_L17_A0.6560.69
150_A159_A0.6560.69
216_Y232_L0.6540.69
100_I311_H0.6540.69
185_F195_S0.6540.69
262_R265_W0.6520.69
185_F189_A0.6480.68
73_A77_E0.6480.68
190_A195_S0.6470.68
264_W273_A0.6470.68
40_I58_W0.6470.68
181_A185_F0.6470.68
49_L122_A0.6460.68
8_A29_R0.6450.68
91_L241_L0.6450.68
119_A261_L0.6430.68
51_D124_R0.6410.67
79_L248_L0.6370.67
6_P113_E0.6360.67
154_L158_A0.6350.67
185_F269_A0.6340.66
60_A64_R0.6340.66
4_H236_T0.6340.66
284_E287_A0.6310.66
7_M11_A0.6270.66
126_Q130_G0.6240.65
181_A184_E0.6230.65
200_D203_A0.6230.65
19_D24_R0.6220.65
308_G316_V0.6220.65
25_A28_R0.6210.65
146_G151_L0.6200.65
8_A12_L0.6190.65
234_A271_L0.6190.65
71_W111_P0.6160.64
98_G125_Y0.6140.64
2_H7_M0.6120.64
209_A216_Y0.6100.63
7_M116_E0.5990.62
234_A243_L0.5990.62
10_E17_A0.5970.62
188_Y196_P0.5950.62
295_P300_L0.5950.62
156_R159_A0.5930.61
146_G154_L0.5930.61
5_L10_E0.5920.61
266_Q270_A0.5900.61
160_D164_A0.5900.61
267_A271_L0.5890.61
153_L165_G0.5890.61
229_F233_H0.5870.61
72_R76_A0.5870.61
15_L242_R0.5850.60
157_V188_Y0.5840.60
181_A186_L0.5840.60
265_W270_A0.5830.60
210_E214_R0.5820.60
301_I316_V0.5820.60
148_T161_P0.5810.60
41_T61_F0.5800.60
182_G195_S0.5790.59
5_L17_A0.5790.59
9_L132_F0.5780.59
51_D54_R0.5770.59
212_A217_L0.5750.59
56_A59_L0.5730.59
56_A238_P0.5730.59
51_D58_W0.5730.59
194_V198_A0.5710.58
90_G125_Y0.5700.58
10_E317_R0.5700.58
272_Y279_P0.5660.58
95_A126_Q0.5640.57
67_A156_R0.5620.57
56_A60_A0.5620.57
31_E34_R0.5620.57
232_L315_L0.5620.57
159_A163_L0.5610.57
229_F232_L0.5610.57
185_F198_A0.5610.57
116_E119_A0.5610.57
262_R268_A0.5590.57
45_W61_F0.5580.57
100_I236_T0.5540.56
7_M230_F0.5520.56
88_L252_L0.5500.56
46_R49_L0.5450.55
17_A229_F0.5450.55
40_I48_A0.5440.55
205_L209_A0.5440.55
30_L269_A0.5440.55
6_P11_A0.5440.55
186_L192_W0.5420.55
199_G250_D0.5410.54
146_G149_S0.5360.54
124_R237_S0.5350.54
169_D172_K0.5350.54
59_L119_A0.5300.53
55_Y124_R0.5290.53
6_P101_R0.5290.53
261_L273_A0.5280.53
55_Y116_E0.5280.53
174_R179_A0.5270.53
189_A194_V0.5260.52
26_Y33_Q0.5250.52
261_L274_A0.5250.52
192_W204_K0.5230.52
145_P149_S0.5230.52
189_A193_D0.5220.52
183_P187_E0.5190.51
70_G73_A0.5190.51
212_A234_A0.5190.51
2_H94_G0.5180.51
154_L159_A0.5150.51
99_L241_L0.5130.51
147_S153_L0.5120.50
90_G240_A0.5110.50
282_P287_A0.5100.50
2_H120_Y0.5100.50
266_Q272_Y0.5090.50
183_P189_A0.5090.50
59_L115_A0.5090.50
155_A160_D0.5080.50
104_H107_E0.5080.50
226_G231_L0.5080.50
98_G117_A0.5070.50
206_A210_E0.5070.50
266_Q269_A0.5060.50
145_P169_D0.5060.50
272_Y277_R0.5030.49
103_G232_L0.5000.49
212_A316_V0.5000.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3nrhA 3 0.3781 27.5 0.96 Contact Map
1p8cA 6 0.3656 16.9 0.964 Contact Map
2d0oB 2 0.0938 4.5 0.972 Contact Map
2qeuA 6 0.3812 4.2 0.972 Contact Map
2af7A 6 0.1562 4.1 0.973 Contact Map
2q0tA 3 0.1719 3.3 0.974 Contact Map
2z1cA 1 0.2313 3.1 0.974 Contact Map
2xu8A 3 0.1656 3 0.974 Contact Map
3myfA 3 0.3625 2.9 0.975 Contact Map
4wv4B 1 0.1094 2.5 0.975 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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