GREMLIN Database
DUF4238 - Protein of unknown function (DUF4238)
PFAM: PF14022 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 271 (258)
Sequences: 6251 (5918)
Seq/√Len: 368.5
META: 0.823

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
1_R41_Y5.6861.00
9_Y190_T4.2111.00
77_K92_E4.1691.00
8_F72_A3.5681.00
40_A266_Y3.2821.00
12_R79_L3.0891.00
44_D47_T2.8171.00
65_S69_G2.6741.00
181_R224_L2.5631.00
221_S224_L2.4131.00
7_Q36_P2.3741.00
7_Q37_K2.3551.00
191_S257_I2.3431.00
225_A253_I2.3201.00
7_Q11_K2.2841.00
182_N188_F2.2541.00
42_E108_P2.2441.00
103_Q218_F2.1441.00
10_L190_T2.0651.00
13_F222_P2.0111.00
93_R97_A1.9761.00
12_R222_P1.9551.00
25_D263_R1.9491.00
72_A76_R1.9411.00
14_A36_P1.9241.00
100_I228_L1.9181.00
47_T58_D1.9151.00
61_E65_S1.9131.00
103_Q107_T1.9051.00
8_F71_A1.8991.00
70_K95_A1.8951.00
45_F111_R1.8641.00
177_W228_L1.8571.00
97_A177_W1.7951.00
4_Y108_P1.7831.00
245_L249_E1.7751.00
15_D33_R1.7441.00
34_T39_V1.7001.00
38_N41_Y1.6891.00
12_R76_R1.6701.00
76_R80_S1.6591.00
71_A75_L1.6201.00
89_T92_E1.6041.00
246_S249_E1.5721.00
21_I36_P1.5611.00
20_K33_R1.5601.00
1_R37_K1.5271.00
175_M230_D1.5211.00
35_S38_N1.5201.00
2_Q47_T1.5161.00
100_I218_F1.5061.00
6_P103_Q1.4991.00
2_Q61_E1.4921.00
5_V103_Q1.4921.00
2_Q46_Y1.4851.00
23_V32_F1.4851.00
182_N250_V1.4801.00
222_P268_S1.4791.00
21_I266_Y1.4641.00
217_Y225_A1.4391.00
19_G36_P1.4351.00
182_N187_P1.4341.00
34_T38_N1.4311.00
107_T193_N1.4031.00
23_V34_T1.3951.00
188_F245_L1.3721.00
39_V266_Y1.3531.00
109_A193_N1.3451.00
188_F225_A1.3221.00
25_D28_T1.3181.00
104_L197_L1.3161.00
180_L227_V1.2941.00
98_R169_A1.2941.00
45_F107_T1.2891.00
74_A95_A1.2851.00
208_G211_P1.2771.00
190_T257_I1.2731.00
36_P41_Y1.2701.00
79_L224_L1.2691.00
141_D144_K1.2661.00
211_P214_A1.2591.00
217_Y257_I1.2581.00
180_L245_L1.2311.00
258_V265_V1.2241.00
179_L224_L1.2231.00
187_P223_R1.2191.00
79_L221_S1.1961.00
4_Y45_F1.1911.00
8_F75_L1.1821.00
4_Y40_A1.1821.00
71_A99_F1.1811.00
151_E154_E1.1771.00
69_G73_P1.1741.00
71_A74_A1.1711.00
100_I195_V1.1701.00
61_E64_L1.1691.00
15_D18_D1.1491.00
2_Q44_D1.1481.00
8_F99_F1.1361.00
187_P268_S1.1361.00
177_W226_L1.1311.00
47_T57_D1.1291.00
45_F108_P1.1211.00
255_R259_A1.1111.00
182_N186_S1.1101.00
70_K73_P1.0851.00
11_K36_P1.0771.00
182_N223_R1.0771.00
180_L225_A1.0751.00
147_L150_K1.0741.00
9_Y218_F1.0641.00
182_N185_G1.0621.00
117_E121_L1.0591.00
94_E173_L1.0561.00
10_L40_A1.0551.00
134_E137_D1.0551.00
209_L212_G1.0541.00
191_S194_P1.0531.00
183_P244_K1.0531.00
149_L153_L1.0461.00
8_F68_E1.0401.00
96_L226_L1.0351.00
74_A92_E1.0341.00
1_R38_N1.0331.00
64_L102_L1.0281.00
6_P46_Y1.0211.00
12_R75_L1.0051.00
210_G214_A0.9931.00
50_D55_E0.9921.00
109_A262_E0.9841.00
99_F103_Q0.9841.00
68_E71_A0.9831.00
252_E255_R0.9791.00
149_L152_D0.9791.00
2_Q45_F0.9761.00
121_L125_Y0.9671.00
5_V40_A0.9561.00
188_F250_V0.9551.00
208_G213_G0.9511.00
200_P203_P0.9431.00
6_P106_R0.9421.00
9_Y191_S0.9411.00
22_W33_R0.9381.00
192_D261_A0.9361.00
119_K123_E0.9361.00
184_T187_P0.9351.00
210_G213_G0.9311.00
51_E54_G0.9241.00
145_K148_D0.9221.00
245_L250_V0.9121.00
150_K153_L0.9081.00
147_L151_E0.9071.00
73_P77_K0.9051.00
12_R221_S0.9021.00
208_G212_G0.8971.00
56_D62_K0.8971.00
190_T266_Y0.8941.00
120_E124_E0.8941.00
21_I26_K0.8811.00
189_I258_V0.8801.00
101_A105_L0.8791.00
88_L92_E0.8761.00
208_G214_A0.8721.00
158_L161_M0.8691.00
139_L143_L0.8691.00
141_D145_K0.8671.00
148_D151_E0.8661.00
146_I149_L0.8581.00
178_T227_V0.8561.00
75_L220_L0.8511.00
181_R223_R0.8471.00
65_S68_E0.8461.00
147_L153_L0.8451.00
166_E170_P0.8441.00
10_L36_P0.8431.00
186_S247_E0.8431.00
97_A173_L0.8421.00
144_K147_L0.8411.00
23_V39_V0.8391.00
118_L122_L0.8381.00
209_L213_G0.8331.00
63_F66_K0.8301.00
140_L149_L0.8291.00
131_R135_L0.8271.00
10_L266_Y0.8271.00
126_L130_E0.8261.00
48_I59_E0.8251.00
93_R177_W0.8251.00
58_D62_K0.8181.00
107_T110_F0.8131.00
181_R244_K0.8121.00
176_K231_P0.8071.00
185_G247_E0.8061.00
9_Y194_P0.8061.00
155_E159_L0.8021.00
25_D264_F0.7981.00
8_F12_R0.7981.00
91_E95_A0.7961.00
206_G209_L0.7961.00
1_R43_R0.7951.00
97_A101_A0.7901.00
217_Y256_L0.7851.00
191_S261_A0.7831.00
198_Y215_E0.7821.00
97_A169_A0.7801.00
54_G57_D0.7751.00
26_K261_A0.7751.00
156_L159_L0.7751.00
144_K150_K0.7751.00
24_Y29_G0.7741.00
44_D58_D0.7741.00
117_E123_E0.7691.00
118_L121_L0.7691.00
97_A172_L0.7671.00
47_T56_D0.7671.00
67_I70_K0.7671.00
120_E123_E0.7651.00
215_E256_L0.7631.00
46_Y106_R0.7621.00
253_I257_I0.7621.00
131_R134_E0.7571.00
66_K70_K0.7551.00
181_R242_V0.7541.00
38_N43_R0.7521.00
178_T229_T0.7521.00
24_Y28_T0.7511.00
129_L132_L0.7481.00
249_E253_I0.7461.00
27_K30_K0.7451.00
105_L110_F0.7451.00
189_I254_N0.7391.00
70_K98_R0.7391.00
25_D32_F0.7381.00
220_L226_L0.7371.00
129_L133_E0.7331.00
140_L143_L0.7321.00
14_A18_D0.7311.00
123_E126_L0.7301.00
21_I25_D0.7301.00
22_W25_D0.7271.00
42_E263_R0.7261.00
21_I39_V0.7231.00
139_L145_K0.7221.00
121_L127_R0.7201.00
11_K19_G0.7191.00
14_A21_I0.7151.00
126_L129_L0.7130.99
251_R255_R0.7110.99
40_A264_F0.7090.99
24_Y31_I0.7080.99
74_A96_L0.7070.99
122_L125_Y0.7030.99
183_P250_V0.7020.99
196_V257_I0.7010.99
27_K263_R0.7000.99
116_E119_K0.6990.99
207_G213_G0.6990.99
25_D30_K0.6980.99
98_R102_L0.6970.99
73_P76_R0.6970.99
133_E137_D0.6960.99
172_L228_L0.6950.99
140_L144_K0.6940.99
142_E145_K0.6930.99
9_Y99_F0.6910.99
140_L146_I0.6890.99
6_P99_F0.6890.99
171_L174_D0.6870.99
46_Y64_L0.6870.99
130_E133_E0.6860.99
62_K66_K0.6840.99
250_V254_N0.6790.99
146_I150_K0.6790.99
249_E252_E0.6770.99
4_Y41_Y0.6760.99
15_D19_G0.6760.99
20_K35_S0.6740.99
209_L214_A0.6720.99
117_E120_E0.6670.99
67_I95_A0.6660.99
119_K122_L0.6630.99
207_G210_G0.6610.99
201_P205_R0.6540.99
205_R208_G0.6500.99
48_I51_E0.6470.99
189_I267_S0.6450.99
146_I151_E0.6430.99
183_P186_S0.6420.99
148_D152_D0.6380.99
122_L126_L0.6350.99
172_L216_I0.6330.99
78_L96_L0.6290.99
22_W26_K0.6290.99
5_V190_T0.6280.99
145_K149_L0.6270.99
149_L154_E0.6260.99
37_K41_Y0.6230.99
8_F11_K0.6220.99
139_L142_E0.6200.99
175_M228_L0.6200.99
215_E229_T0.6180.99
19_G23_V0.6170.99
7_Q41_Y0.6150.99
18_D33_R0.6140.98
129_L135_L0.6120.98
143_L147_L0.6120.98
134_E138_E0.6120.98
217_Y253_I0.6110.98
22_W267_S0.6050.98
123_E127_R0.6040.98
247_E251_R0.6030.98
141_D152_D0.5980.98
144_K148_D0.5960.98
195_V216_I0.5950.98
184_T247_E0.5940.98
69_G72_A0.5940.98
9_Y12_R0.5910.98
169_A173_L0.5910.98
50_D54_G0.5890.98
170_P174_D0.5860.98
97_A228_L0.5860.98
216_I228_L0.5840.98
71_A95_A0.5820.98
108_P111_R0.5810.98
94_E169_A0.5770.98
21_I24_Y0.5770.98
167_E170_P0.5760.98
182_N245_L0.5750.98
40_A47_T0.5740.98
2_Q58_D0.5730.98
117_E124_E0.5720.98
4_Y107_T0.5710.98
99_F218_F0.5700.98
28_T32_F0.5690.98
143_L146_I0.5680.98
105_L165_A0.5670.97
225_A257_I0.5670.97
126_L135_L0.5660.97
188_F253_I0.5650.97
183_P223_R0.5650.97
28_T31_I0.5640.97
14_A19_G0.5630.97
157_L161_M0.5570.97
9_Y75_L0.5510.97
67_I102_L0.5510.97
139_L153_L0.5490.97
75_L79_L0.5480.97
138_E142_E0.5470.97
40_A190_T0.5470.97
15_D36_P0.5450.97
143_L150_K0.5440.97
20_K34_T0.5430.97
248_E252_E0.5420.97
146_I153_L0.5380.97
170_P173_L0.5360.96
261_A265_V0.5350.96
198_Y252_E0.5330.96
119_K125_Y0.5330.96
136_L139_L0.5310.96
108_P263_R0.5300.96
162_L165_A0.5280.96
159_L163_E0.5280.96
6_P72_A0.5280.96
133_E136_L0.5260.96
124_E128_E0.5260.96
142_E146_I0.5260.96
103_Q106_R0.5250.96
118_L123_E0.5240.96
145_K150_K0.5240.96
39_V264_F0.5240.96
148_D153_L0.5160.96
143_L148_D0.5120.95
132_L135_L0.5100.95
5_V9_Y0.5100.95
172_L175_M0.5090.95
84_G88_L0.5090.95
256_L260_N0.5080.95
39_V42_E0.5070.95
75_L96_L0.5060.95
98_R165_A0.5060.95
190_T194_P0.5050.95
42_E45_F0.5050.95
252_E256_L0.5040.95
146_I152_D0.5040.95
243_R249_E0.5030.95
140_L148_D0.5020.95
93_R173_L0.5000.95
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2b5uA 1 0.2362 9.6 0.942 Contact Map
1y66A 3 0.1845 7.7 0.945 Contact Map
4u6uA 1 0.2103 7.6 0.945 Contact Map
2krkA 1 0.2657 4 0.952 Contact Map
5aj3h 1 0 2.8 0.955 Contact Map
3bgvA 1 0.3026 2.7 0.956 Contact Map
2mkbA 1 0.0812 2.3 0.958 Contact Map
3i24A 2 0.2768 2 0.959 Contact Map
4jpoC 1 0.2435 2 0.959 Contact Map
1dzfA 1 0.2583 1.9 0.959 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.008 seconds.