GREMLIN Database
DUF4230 - Protein of unknown function (DUF4230)
PFAM: PF14014 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 145 (141)
Sequences: 10862 (7576)
Seq/√Len: 638.0
META: 0.883

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
50_K70_E3.1141.00
68_P124_E2.3611.00
69_P124_E2.3361.00
132_E136_R2.3221.00
10_E43_K2.1011.00
6_L10_E2.0921.00
128_R132_E2.0401.00
124_E128_R2.0301.00
43_K73_S2.0101.00
36_L81_T1.9901.00
121_E125_E1.9691.00
111_R115_L1.9561.00
45_G72_L1.9311.00
107_E111_R1.9251.00
47_D50_K1.8931.00
77_D80_R1.8631.00
109_Q112_K1.8621.00
46_V127_A1.7601.00
37_V82_R1.7291.00
108_E112_K1.7181.00
41_T75_E1.7101.00
37_V84_Y1.7041.00
53_P56_V1.6631.00
39_H80_R1.6341.00
44_A119_L1.5951.00
101_E104_Q1.5911.00
13_E41_T1.5881.00
23_E31_G1.5651.00
100_T104_Q1.5511.00
52_K55_D1.5491.00
98_D102_L1.5241.00
103_R107_E1.5171.00
105_E108_E1.5061.00
40_G110_I1.4931.00
23_E33_R1.4851.00
27_D30_G1.4701.00
122_Q125_E1.4421.00
56_V59_D1.4171.00
101_E105_E1.4151.00
125_E128_R1.4011.00
56_V60_G1.3701.00
44_A123_A1.3671.00
47_D70_E1.3481.00
24_D32_K1.3441.00
105_E109_Q1.3421.00
35_L84_Y1.3381.00
57_E63_T1.3281.00
118_G121_E1.3211.00
99_E103_R1.3091.00
125_E129_A1.2761.00
15_T41_T1.2751.00
121_E124_E1.2731.00
61_D141_K1.2541.00
10_E72_L1.2271.00
74_V111_R1.2151.00
13_E43_K1.2021.00
129_A133_S1.2011.00
17_E39_H1.1941.00
42_V71_I1.1861.00
57_E65_T1.1841.00
112_K116_E1.1751.00
4_R13_E1.1251.00
12_V44_A1.0981.00
104_Q108_E1.0921.00
69_P120_L1.0881.00
8_E43_K1.0801.00
11_T45_G1.0741.00
104_Q107_E1.0691.00
115_L120_L1.0471.00
95_D98_D1.0451.00
129_A132_E1.0311.00
14_Y119_L1.0251.00
86_R99_E1.0121.00
7_S130_L1.0111.00
8_E70_E0.9961.00
78_E107_E0.9801.00
115_L118_G0.9671.00
126_N130_L0.9651.00
19_V84_Y0.9601.00
108_E111_R0.9541.00
51_L55_D0.9501.00
42_V74_V0.9311.00
97_E101_E0.9311.00
19_V35_L0.9311.00
36_L103_R0.9191.00
15_T39_H0.8951.00
56_V61_D0.8921.00
7_S49_S0.8921.00
32_K86_R0.8881.00
64_I135_L0.8841.00
48_L51_L0.8821.00
41_T73_S0.8811.00
72_L75_E0.8771.00
58_V62_V0.8731.00
11_T127_A0.8701.00
99_E102_L0.8671.00
44_A120_L0.8641.00
43_K75_E0.8611.00
44_A71_I0.8601.00
118_G122_Q0.8591.00
109_Q113_A0.8591.00
33_R87_Q0.8571.00
32_K98_D0.8541.00
25_S31_G0.8511.00
78_E103_R0.8501.00
44_A118_G0.8481.00
120_L124_E0.8481.00
26_K30_G0.8431.00
5_D126_N0.8401.00
17_E37_V0.8291.00
18_K109_Q0.8211.00
21_T33_R0.8181.00
42_V114_A0.8131.00
41_T77_D0.8121.00
21_T35_L0.8071.00
98_D103_R0.8041.00
100_T103_R0.8021.00
136_R141_K0.8011.00
10_E13_E0.8001.00
126_N129_A0.7991.00
135_L140_F0.7981.00
75_E107_E0.7961.00
50_K67_P0.7951.00
59_D63_T0.7951.00
52_K65_T0.7831.00
12_V122_Q0.7831.00
113_A116_E0.7801.00
55_D58_V0.7801.00
51_L54_E0.7731.00
46_V69_P0.7731.00
12_V119_L0.7621.00
130_L133_S0.7581.00
19_V37_V0.7501.00
33_R85_D0.7481.00
38_A110_I0.7421.00
89_G93_R0.7401.00
40_G106_A0.7401.00
38_A76_L0.7371.00
55_D65_T0.7351.00
98_D101_E0.7321.00
58_V61_D0.7291.00
8_E45_G0.7221.00
79_D104_Q0.7191.00
38_A106_A0.7181.00
5_D129_A0.7171.00
54_E58_V0.7101.00
62_V135_L0.7081.00
112_K115_L0.7071.00
8_E47_D0.7061.00
44_A69_P0.7051.00
44_A114_A0.7011.00
8_E72_L0.6981.00
75_E78_E0.6961.00
9_L48_L0.6931.00
4_R15_T0.6851.00
106_A110_I0.6771.00
6_L13_E0.6711.00
131_L135_L0.6711.00
35_L85_D0.6691.00
11_T126_N0.6641.00
5_D122_Q0.6581.00
24_D101_E0.6581.00
8_E13_E0.6571.00
133_S137_S0.6571.00
122_Q126_N0.6561.00
130_L134_L0.6521.00
66_L127_A0.6461.00
102_L106_A0.6451.00
53_P58_V0.6411.00
9_L130_L0.6391.00
66_L131_L0.6321.00
88_T92_N0.6291.00
91_L94_F0.6281.00
18_K113_A0.6271.00
16_F42_V0.6261.00
43_K72_L0.6161.00
7_S11_T0.6141.00
114_A119_L0.6141.00
40_G76_L0.6121.00
12_V15_T0.6071.00
18_K38_A0.6031.00
83_V86_R0.5991.00
22_Y102_L0.5981.00
106_A109_Q0.5971.00
39_H81_T0.5881.00
16_F117_S0.5831.00
88_T91_L0.5731.00
32_K92_N0.5661.00
133_S136_R0.5641.00
14_Y44_A0.5641.00
74_V77_D0.5611.00
94_F102_L0.5601.00
22_Y105_E0.5571.00
20_V38_A0.5551.00
103_R106_A0.5541.00
136_R142_E0.5471.00
94_F98_D0.5380.99
64_I131_L0.5350.99
35_L39_H0.5350.99
57_E61_D0.5340.99
58_V140_F0.5300.99
113_A117_S0.5240.99
18_K21_T0.5220.99
83_V100_T0.5200.99
53_P57_E0.5180.99
42_V45_G0.5140.99
38_A81_T0.5120.99
18_K110_I0.5110.99
36_L83_V0.5100.99
76_L107_E0.5070.99
102_L107_E0.5010.99
13_E122_Q0.5000.99
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2jvfA 1 0.5172 9.1 0.907 Contact Map
3h4zA 1 0.8483 8.8 0.908 Contact Map
2ck3A 1 0.9241 6.8 0.912 Contact Map
3gkuA 2 0.5379 6.7 0.912 Contact Map
3uv1A 2 0.8207 6.2 0.914 Contact Map
1usuB 1 0.8 6 0.915 Contact Map
1qysA 1 0.5034 5.7 0.915 Contact Map
3iibA 2 0.3793 4.7 0.919 Contact Map
4gfqA 1 0.8828 4.2 0.921 Contact Map
2gljA 6 0.4 4.1 0.921 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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