GREMLIN Database
DUF4220 - Domain of unknown function (DUF4220)
PFAM: PF13968 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 315 (295)
Sequences: 1434 (1184)
Seq/√Len: 68.9
META: 0.041

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
9_S12_T3.4581.00
1_W4_Y3.2521.00
122_M125_Y3.1891.00
257_V261_I3.1121.00
117_V120_Y2.7201.00
160_F163_F2.6501.00
105_W109_S2.5991.00
163_F166_L2.3831.00
224_R227_S2.3301.00
16_G19_S2.2851.00
238_L242_S2.2781.00
124_G127_S2.2041.00
28_F266_L2.1981.00
184_S188_F2.1331.00
236_L240_A2.1121.00
205_E208_F2.1061.00
144_S147_S2.0801.00
283_S286_T2.0691.00
129_A132_V1.9991.00
163_F167_K1.9931.00
116_L120_Y1.9881.00
181_L185_R1.9731.00
150_G155_D1.9681.00
286_T290_L1.9171.00
13_Y33_L1.8891.00
227_S231_T1.8801.00
224_R228_L1.8641.00
28_F92_L1.8491.00
115_K118_A1.8291.00
47_S55_L1.8091.00
118_A121_M1.8051.00
228_L231_T1.8051.00
8_D57_H1.7701.00
159_A167_K1.7591.00
155_D197_R1.7521.00
114_S117_V1.7281.00
181_L184_S1.7251.00
28_F32_F1.7061.00
260_A264_V1.6971.00
185_R189_L1.6921.00
158_L161_A1.6911.00
54_W57_H1.6891.00
57_H64_Q1.6730.99
18_L31_P1.6680.99
8_D12_T1.6610.99
79_L84_I1.6380.99
32_F35_L1.6370.99
32_F48_L1.6210.99
61_L64_Q1.6200.99
31_P36_H1.6070.99
13_Y19_S1.5900.99
43_I46_Y1.5880.99
122_M126_R1.5870.99
300_R304_G1.5810.99
182_D185_R1.5720.99
178_E181_L1.5610.99
205_E211_D1.5390.99
156_L163_F1.5380.99
234_A238_L1.5230.99
165_L209_M1.5220.99
12_T60_T1.5220.99
238_L241_L1.5050.99
122_M128_S1.5030.99
208_F211_D1.5020.99
79_L82_A1.4950.99
235_A239_F1.4910.99
224_R239_F1.4770.99
15_L68_A1.4710.99
45_A56_R1.4630.99
147_S153_L1.4580.99
45_A50_D1.4570.99
47_S50_D1.4440.98
237_V241_L1.4420.98
102_E216_K1.4380.98
165_L168_R1.4370.98
142_L145_P1.4250.98
93_F96_G1.4150.98
11_A15_L1.4080.98
163_F208_F1.4040.98
31_P37_L1.4040.98
50_D54_W1.3940.98
159_A204_V1.3940.98
38_G103_R1.3880.98
53_L56_R1.3790.98
115_K119_D1.3770.98
96_G100_Y1.3740.98
6_L13_Y1.3680.98
181_L188_F1.3500.98
7_A11_A1.3460.98
44_T51_N1.3320.97
225_L228_L1.3320.97
208_F284_D1.3310.97
141_S145_P1.3290.97
64_Q70_Y1.3160.97
34_L37_L1.3150.97
264_V279_M1.3110.97
51_N56_R1.2890.97
207_S211_D1.2790.97
217_A222_T1.2760.97
160_F167_K1.2740.97
172_D179_A1.2740.97
161_A165_L1.2690.97
106_A110_A1.2680.96
146_D151_P1.2450.96
292_C295_V1.2430.96
142_L147_S1.2420.96
35_L40_P1.2310.96
13_Y17_L1.2260.96
161_A164_K1.2170.96
7_A19_S1.2160.96
121_M125_Y1.2160.96
170_F173_L1.2130.95
2_L6_L1.2120.95
8_D31_P1.2110.95
201_V205_E1.2100.95
50_D56_R1.2100.95
293_K296_R1.2090.95
18_L26_V1.2050.95
199_F208_F1.2050.95
199_F205_E1.1970.95
101_G105_W1.1970.95
180_D184_S1.1960.95
69_L72_F1.1940.95
25_L258_D1.1920.95
57_H104_T1.1920.95
41_D44_T1.1910.95
136_K140_C1.1860.95
1_W56_R1.1760.95
186_D190_K1.1720.95
36_H39_G1.1720.95
147_S152_R1.1700.95
7_A16_G1.1700.95
133_T138_W1.1660.94
183_K187_F1.1630.94
38_G54_W1.1630.94
98_I156_L1.1330.94
236_L266_L1.1320.93
26_V72_F1.1290.93
183_K186_D1.1230.93
114_S120_Y1.1200.93
49_E107_L1.1190.93
179_A184_S1.1050.93
15_L308_Q1.0990.92
44_T50_D1.0940.92
227_S230_L1.0900.92
56_R64_Q1.0860.92
17_L26_V1.0780.92
203_E211_D1.0780.92
135_D139_E1.0610.91
200_K203_E1.0570.91
116_L121_M1.0410.90
46_Y50_D1.0370.90
193_L197_R1.0360.90
168_R178_E1.0350.90
65_V69_L1.0340.90
4_Y50_D1.0210.89
57_H61_L1.0150.89
8_D36_H1.0120.89
107_L111_S1.0110.89
159_A172_D1.0100.89
224_R231_T1.0080.89
37_L44_T1.0070.89
18_L74_K1.0060.89
80_L84_I1.0040.89
82_A85_L0.9980.88
166_L169_L0.9960.88
228_L277_L0.9940.88
76_W221_H0.9920.88
163_F205_E0.9910.88
45_A53_L0.9890.88
134_V138_W0.9880.88
224_R230_L0.9850.88
46_Y56_R0.9830.87
169_L172_D0.9810.87
306_I309_Y0.9800.87
37_L214_Y0.9760.87
123_E126_R0.9700.87
228_L234_A0.9690.87
103_R109_S0.9650.87
117_V121_M0.9630.86
165_L169_L0.9580.86
234_A242_S0.9430.85
30_A39_G0.9340.85
4_Y216_K0.9250.84
49_E56_R0.9240.84
200_K234_A0.9190.84
121_M124_G0.9190.84
73_Y284_D0.9170.84
10_V63_V0.9160.84
177_S181_L0.9160.84
167_K205_E0.9070.83
14_A171_V0.9020.83
54_W64_Q0.9000.82
174_I199_F0.8980.82
107_L266_L0.8960.82
6_L264_V0.8940.82
13_Y80_L0.8920.82
158_L168_R0.8860.81
115_K121_M0.8810.81
156_L165_L0.8770.81
96_G102_E0.8760.81
44_T49_E0.8670.80
298_R308_Q0.8670.80
45_A214_Y0.8650.80
12_T15_L0.8650.80
50_D215_T0.8650.80
44_T57_H0.8640.80
58_L61_L0.8630.80
199_F287_K0.8620.80
11_A57_H0.8580.79
36_H49_E0.8580.79
54_W58_L0.8570.79
143_L151_P0.8500.79
133_T140_C0.8470.78
142_L146_D0.8470.78
305_S310_N0.8470.78
3_A13_Y0.8430.78
251_K254_Y0.8430.78
163_F171_V0.8430.78
136_K147_S0.8360.77
155_D158_L0.8360.77
143_L147_S0.8330.77
115_K120_Y0.8280.77
148_D152_R0.8280.77
2_L5_L0.8260.77
265_L269_A0.8260.77
170_F186_D0.8240.76
79_L83_A0.8210.76
52_E261_I0.8200.76
35_L92_L0.8190.76
285_W288_V0.8150.76
66_A69_L0.8140.76
182_D186_D0.8120.75
146_D149_S0.8110.75
8_D54_W0.8100.75
136_K139_E0.8090.75
14_A17_L0.8040.75
99_K103_R0.8000.74
18_L68_A0.7970.74
248_D251_K0.7970.74
34_L39_G0.7960.74
61_L100_Y0.7940.74
1_W5_L0.7900.73
220_L269_A0.7850.73
203_E302_W0.7840.73
51_N54_W0.7830.73
179_A186_D0.7810.73
159_A162_L0.7750.72
13_Y71_V0.7730.72
165_L178_E0.7730.72
211_D214_Y0.7720.72
280_L284_D0.7690.71
155_D188_F0.7680.71
156_L204_V0.7620.71
209_M213_L0.7610.71
212_F218_P0.7610.71
180_D188_F0.7580.70
59_L104_T0.7580.70
151_P154_K0.7580.70
129_A147_S0.7580.70
110_A212_F0.7570.70
277_L282_F0.7570.70
145_P150_G0.7570.70
147_S151_P0.7560.70
166_L174_I0.7540.70
42_T210_Y0.7520.70
4_Y37_L0.7510.70
32_F266_L0.7500.69
38_G44_T0.7490.69
55_L65_V0.7450.69
116_L119_D0.7440.69
226_I230_L0.7440.69
36_H40_P0.7430.69
101_G196_E0.7410.69
8_D32_F0.7380.68
284_D301_R0.7350.68
42_T216_K0.7290.67
285_W290_L0.7270.67
134_V137_I0.7240.67
214_Y303_S0.7230.67
160_F166_L0.7230.67
39_G44_T0.7210.66
7_A13_Y0.7210.66
162_L168_R0.7200.66
11_A37_L0.7160.66
29_W36_H0.7160.66
147_S150_G0.7120.65
185_R188_F0.7100.65
48_L75_S0.7080.65
205_E285_W0.7060.65
31_P40_P0.7040.65
105_W277_L0.7010.64
141_S148_D0.6990.64
3_A66_A0.6970.64
153_L196_E0.6950.64
187_F202_I0.6950.64
7_A100_Y0.6900.63
157_C160_F0.6900.63
51_N110_A0.6870.63
282_F287_K0.6870.63
177_S188_F0.6870.63
4_Y8_D0.6840.62
100_Y104_T0.6830.62
61_L65_V0.6800.62
169_L174_I0.6780.62
141_S151_P0.6770.62
276_S283_S0.6760.61
30_A227_S0.6750.61
7_A269_A0.6740.61
172_D204_V0.6720.61
207_S304_G0.6700.61
156_L162_L0.6690.61
13_Y47_S0.6680.61
95_V237_V0.6650.60
94_V298_R0.6640.60
43_I50_D0.6630.60
106_A200_K0.6630.60
202_I263_Y0.6620.60
157_C164_K0.6610.60
207_S275_Y0.6600.60
139_E143_L0.6570.59
213_L216_K0.6550.59
19_S39_G0.6510.59
38_G45_A0.6500.58
121_M137_I0.6500.58
37_L45_A0.6490.58
265_L276_S0.6490.58
157_C161_A0.6470.58
149_S154_K0.6460.58
51_N75_S0.6420.58
60_T73_Y0.6360.57
282_F307_G0.6360.57
45_A64_Q0.6360.57
142_L151_P0.6350.57
178_E185_R0.6350.57
41_D56_R0.6310.56
12_T17_L0.6290.56
170_F211_D0.6270.56
119_D216_K0.6270.56
184_S191_G0.6270.56
144_S148_D0.6260.56
31_P34_L0.6260.56
270_I278_L0.6250.55
26_V225_L0.6210.55
8_D11_A0.6170.55
210_Y215_T0.6160.54
41_D99_K0.6140.54
96_G99_K0.6130.54
94_V158_L0.6120.54
199_F211_D0.6100.54
3_A162_L0.6080.53
125_Y128_S0.6070.53
19_S26_V0.6060.53
259_V263_Y0.6030.53
211_D273_E0.6020.53
44_T215_T0.6020.53
164_K179_A0.6010.53
3_A309_Y0.6010.53
100_Y213_L0.5970.52
185_R306_I0.5970.52
37_L49_E0.5960.52
57_H60_T0.5950.52
199_F288_V0.5950.52
69_L97_I0.5940.52
174_I177_S0.5930.52
38_G96_G0.5910.51
83_A94_V0.5910.51
187_F197_R0.5880.51
171_V177_S0.5840.51
114_S118_A0.5830.50
92_L231_T0.5820.50
213_L219_V0.5820.50
96_G263_Y0.5820.50
16_G58_L0.5820.50
160_F208_F0.5800.50
258_D272_L0.5800.50
34_L43_I0.5790.50
143_L148_D0.5790.50
214_Y241_L0.5780.50
234_A240_A0.5760.50
10_V276_S0.5720.49
140_C148_D0.5720.49
149_S152_R0.5700.49
166_L208_F0.5690.49
169_L173_L0.5690.49
128_S134_V0.5680.49
169_L197_R0.5680.49
32_F61_L0.5680.49
164_K167_K0.5670.49
5_L8_D0.5660.48
33_L55_L0.5660.48
292_C296_R0.5640.48
164_K168_R0.5630.48
150_G153_L0.5630.48
71_V99_K0.5620.48
148_D151_P0.5610.48
8_D37_L0.5590.48
63_V312_L0.5590.48
102_E106_A0.5580.47
138_W159_A0.5580.47
37_L50_D0.5520.47
124_G154_K0.5510.47
68_A72_F0.5490.46
12_T108_R0.5480.46
260_A271_L0.5470.46
53_L96_G0.5460.46
158_L166_L0.5460.46
219_V222_T0.5450.46
198_A203_E0.5430.46
159_A211_D0.5430.46
49_E54_W0.5410.45
178_E206_L0.5400.45
32_F37_L0.5400.45
216_K268_G0.5370.45
152_R155_D0.5360.45
92_L241_L0.5330.44
122_M127_S0.5330.44
148_D153_L0.5330.44
1_W64_Q0.5320.44
141_S278_L0.5320.44
105_W266_L0.5320.44
11_A64_Q0.5270.44
229_L234_A0.5240.43
182_D196_E0.5230.43
40_P47_S0.5230.43
68_A308_Q0.5200.43
307_G313_S0.5190.43
94_V98_I0.5180.43
46_Y82_A0.5160.42
188_F191_G0.5160.42
200_K301_R0.5140.42
65_V72_F0.5130.42
81_A85_L0.5130.42
212_F285_W0.5130.42
44_T48_L0.5110.42
33_L37_L0.5110.42
169_L177_S0.5100.42
44_T107_L0.5090.42
40_P163_F0.5080.42
96_G216_K0.5040.41
153_L306_I0.5000.41
40_P48_L0.5000.41
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3zd0A 1 0.1524 5.4 0.974 Contact Map
4lx3B 1 0.1143 3.2 0.977 Contact Map
2j9uA 1 0.2571 2.4 0.978 Contact Map
2kebA 1 0.1079 1.9 0.98 Contact Map
2j9wA 1 0.2794 1.6 0.98 Contact Map
2mtsA 1 0.1397 1.5 0.981 Contact Map
4ysxD 1 0.2984 1.5 0.981 Contact Map
2ab9A 1 0.0984 1.4 0.981 Contact Map
4ytpD 1 0.2889 1.2 0.982 Contact Map
4xtrG 1 0.0698 1.1 0.982 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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