GREMLIN Database
DUF4218 - Domain of unknown function (DUF4218)
PFAM: PF13960 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 125 (122)
Sequences: 961 (626)
Seq/√Len: 56.7
META: 0.496

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
26_E60_R4.6901.00
26_E57_D4.0841.00
62_G68_R3.7531.00
13_C69_W3.2711.00
83_Y101_E2.7701.00
23_E60_R2.6521.00
4_E35_K2.5641.00
76_Y80_L2.5551.00
17_I59_A2.5261.00
73_F77_M2.5131.00
29_I52_P2.2511.00
78_K82_G2.1591.00
117_I120_P2.1001.00
79_V83_Y2.0551.00
3_T7_N1.7960.99
22_L56_V1.6750.99
4_E28_E1.6480.99
114_V118_G1.6440.99
25_L29_I1.6180.99
46_D49_V1.5090.98
58_E68_R1.4590.97
84_V92_G1.4540.97
105_T109_R1.4330.97
40_F48_M1.4230.97
17_I71_Y1.3890.96
116_T119_N1.3880.96
7_N10_R1.3760.96
93_S97_G1.3650.96
92_G96_E1.3510.96
65_V69_W1.3400.95
109_R113_D1.3310.95
105_T118_G1.3010.95
119_N122_S1.2600.94
42_P45_F1.2570.94
114_V117_I1.2130.92
46_D52_P1.2130.92
75_R91_E1.2050.92
2_I6_C1.2030.92
17_I22_L1.1690.91
26_E56_V1.1350.90
44_F58_E1.1290.89
47_I90_P1.1260.89
114_V122_S1.1210.89
56_V59_A1.0990.88
115_E122_S1.0890.88
33_L49_V1.0890.88
14_S17_I1.0860.87
18_D21_D1.0820.87
50_H63_G1.0770.87
113_D117_I1.0730.87
57_D61_L1.0540.86
98_Y107_C1.0420.85
112_E121_P1.0350.85
49_V52_P1.0230.84
20_E27_K1.0230.84
118_G121_P1.0100.83
15_K22_L1.0080.83
101_E122_S1.0040.83
105_T108_S0.9920.82
7_N11_A0.9820.81
2_I53_V0.9810.81
36_L46_D0.9710.81
109_R118_G0.9710.81
85_R97_G0.9700.81
45_F48_M0.9700.81
108_S111_L0.9670.80
118_G122_S0.9610.80
52_P56_V0.9540.80
115_E121_P0.9530.80
4_E31_E0.9510.79
93_S98_Y0.9440.79
2_I5_L0.9400.79
115_E119_N0.9390.78
114_V119_N0.9250.77
9_F46_D0.9080.76
44_F49_V0.8950.75
111_L114_V0.8940.75
115_E118_G0.8890.75
34_C37_E0.8860.74
79_V101_E0.8720.73
27_K31_E0.8670.73
59_A67_Y0.8500.71
17_I28_E0.8430.71
37_E42_P0.8340.70
113_D119_N0.8260.69
29_I32_T0.8220.69
58_E71_Y0.8140.68
84_V88_A0.8110.68
103_C107_C0.8090.67
105_T112_E0.8090.67
115_E120_P0.8070.67
2_I14_S0.7900.66
42_P58_E0.7810.65
114_V121_P0.7800.65
22_L90_P0.7780.64
70_M98_Y0.7740.64
2_I92_G0.7710.64
33_L45_F0.7700.64
63_G74_E0.7690.63
26_E30_V0.7600.63
109_R116_T0.7530.62
112_E117_I0.7510.62
3_T12_L0.7360.60
112_E123_R0.7330.60
24_K76_Y0.7240.59
108_S120_P0.7230.59
28_E71_Y0.7190.58
104_L108_S0.7110.57
109_R112_E0.7080.57
40_F72_P0.7070.57
35_K38_K0.7030.57
31_E35_K0.7010.56
8_F29_I0.7000.56
107_C112_E0.6990.56
4_E14_S0.6980.56
117_I122_S0.6950.56
15_K56_V0.6930.55
117_I121_P0.6880.55
39_I119_N0.6870.55
84_V90_P0.6810.54
46_D82_G0.6800.54
67_Y77_M0.6760.54
5_L52_P0.6740.53
11_A25_L0.6690.53
97_G101_E0.6670.53
104_L118_G0.6610.52
23_E56_V0.6600.52
112_E120_P0.6540.51
51_L91_E0.6540.51
8_F36_L0.6490.51
10_R79_V0.6470.50
104_L111_L0.6430.50
21_D93_S0.6350.49
116_T120_P0.6230.48
25_L45_F0.6180.47
49_V56_V0.6150.47
83_Y107_C0.6120.47
119_N123_R0.6090.46
52_P117_I0.6070.46
10_R13_C0.6050.46
78_K87_R0.6050.46
64_P71_Y0.6010.45
34_C38_K0.5970.45
33_L40_F0.5970.45
12_L66_Q0.5970.45
28_E62_G0.5930.45
109_R115_E0.5930.45
79_V97_G0.5820.43
3_T97_G0.5770.43
29_I114_V0.5750.43
16_V59_A0.5750.43
104_L107_C0.5750.43
36_L45_F0.5740.43
78_K103_C0.5740.43
87_R90_P0.5730.42
3_T21_D0.5730.42
10_R18_D0.5680.42
72_P94_I0.5660.42
33_L43_S0.5660.42
71_Y74_E0.5650.42
105_T116_T0.5630.41
63_G123_R0.5620.41
69_W73_F0.5620.41
22_L26_E0.5590.41
86_N93_S0.5480.40
70_M90_P0.5460.40
104_L121_P0.5440.39
70_M75_R0.5420.39
12_L59_A0.5420.39
71_Y96_E0.5420.39
42_P86_N0.5390.39
64_P78_K0.5390.39
101_E118_G0.5330.38
83_Y90_P0.5290.38
111_L115_E0.5260.38
28_E32_T0.5230.37
36_L43_S0.5170.37
72_P79_V0.5170.37
105_T120_P0.5120.36
38_K88_A0.5080.36
53_V61_L0.5050.35
31_E82_G0.5030.35
54_H63_G0.5000.35
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4i77Z 1 0.36 6.9 0.936 Contact Map
3s51A 4 0.928 6.8 0.936 Contact Map
4pjwA 1 0.832 5.8 0.938 Contact Map
4ne3B 2 0.376 5.3 0.939 Contact Map
4aikA 2 0.328 5 0.94 Contact Map
1vsy4 1 0.376 4.9 0.94 Contact Map
4e45B 2 0.376 4.4 0.942 Contact Map
4amsA 1 0.536 4.1 0.942 Contact Map
1w53A 2 0.472 4.1 0.942 Contact Map
1hy5A 1 0.352 4 0.943 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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