GREMLIN Database
DUF4216 - Domain of unknown function (DUF4216)
PFAM: PF13952 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 70 (69)
Sequences: 1777 (1138)
Seq/√Len: 137.0
META: 0.701

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
48_I53_A4.8861.00
19_F37_L3.7271.00
17_D40_L2.6551.00
37_L68_H2.1381.00
2_E10_K1.9911.00
4_D9_F1.9891.00
25_V33_T1.9431.00
60_P66_N1.8771.00
15_K46_P1.7511.00
41_G45_D1.6771.00
19_F40_L1.6151.00
65_K68_H1.5991.00
36_N66_N1.4601.00
60_P65_K1.4501.00
3_L11_V1.4311.00
6_G10_K1.4181.00
13_L46_P1.4151.00
28_D31_G1.3931.00
1_I47_F1.3741.00
34_L41_G1.3731.00
60_P68_H1.3471.00
16_C20_D1.3231.00
59_V64_D1.2941.00
19_F35_V1.2751.00
6_G17_D1.2351.00
49_L52_Q1.2291.00
60_P63_S1.2011.00
32_F36_N1.1330.99
26_K34_L1.1190.99
45_D48_I1.1170.99
40_L44_D1.1150.99
24_G50_A1.1140.99
1_I36_N1.0730.99
16_C24_G1.0600.99
36_N41_G1.0280.99
21_K27_V1.0000.98
47_F68_H0.9880.98
50_A55_Q0.9810.98
4_D10_K0.9750.98
29_K32_F0.9640.98
6_G9_F0.9470.98
4_D8_E0.9390.97
21_K29_K0.9090.97
61_D65_K0.9010.97
57_F61_D0.8860.96
32_F59_V0.8800.96
42_Y45_D0.8590.96
26_K64_D0.8400.95
12_V19_F0.8360.95
38_N50_A0.8070.94
66_N69_V0.7740.93
34_L57_F0.7630.92
20_D39_R0.7490.91
48_I69_V0.7490.91
25_V44_D0.7460.91
10_K41_G0.7380.91
34_L61_D0.7360.91
1_I41_G0.7350.91
2_E9_F0.7220.90
27_V30_Y0.7160.89
17_D45_D0.7160.89
9_F39_R0.7140.89
5_Y44_D0.6950.88
42_Y49_L0.6940.88
54_T58_Y0.6780.87
8_E30_Y0.6680.86
1_I33_T0.6680.86
39_R51_S0.6650.86
51_S54_T0.6650.86
24_G58_Y0.6600.85
9_F12_V0.6550.85
31_G64_D0.6490.84
33_T37_L0.6470.84
14_F58_Y0.6450.84
34_L69_V0.6330.83
4_D7_G0.6290.82
7_G54_T0.6290.82
16_C56_V0.6180.81
19_F69_V0.6150.81
7_G12_V0.6100.80
26_K38_N0.5790.77
13_L16_C0.5750.76
21_K48_I0.5750.76
50_A53_A0.5710.76
30_Y42_Y0.5700.76
61_D64_D0.5660.75
3_L40_L0.5630.75
23_R38_N0.5550.74
12_V37_L0.5500.73
1_I12_V0.5490.73
35_V62_P0.5420.72
12_V26_K0.5410.72
28_D50_A0.5270.70
7_G62_P0.5250.70
37_L41_G0.5220.69
24_G66_N0.5210.69
34_L67_W0.5130.68
38_N43_K0.5120.68
31_G37_L0.5100.67
45_D56_V0.5100.67
49_L63_S0.5090.67
1_I7_G0.5010.66
4_D12_V0.5010.66
20_D46_P0.5000.66
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2mwiA 1 0.6 13.8 0.893 Contact Map
4mhlA 1 0.4143 3 0.922 Contact Map
4qfuA 5 0.7286 2.7 0.923 Contact Map
4bgdA 1 0.7429 2.4 0.925 Contact Map
4my6A 1 0.3857 2 0.928 Contact Map
2e1bA 1 0.4429 2 0.929 Contact Map
2kcmA 1 0.7 1.9 0.929 Contact Map
2fgtA 1 0.9 1.6 0.932 Contact Map
4f92B 1 0.7 1.6 0.933 Contact Map
3winC 1 0.9857 1.5 0.933 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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