GREMLIN Database
DUF4199 - Protein of unknown function (DUF4199)
PFAM: PF13858 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 168 (163)
Sequences: 11891 (10209)
Seq/√Len: 799.7
META: 0.917

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
64_I69_A2.9431.00
11_I87_F2.5821.00
53_K57_D2.4471.00
6_L80_A2.3921.00
51_G76_I2.2461.00
77_S155_G2.1871.00
127_T130_Q2.0891.00
91_Y96_D2.0501.00
54_S58_N1.9591.00
108_I112_E1.9551.00
89_Y93_N1.9331.00
73_G159_S1.9211.00
105_E109_E1.8611.00
46_V50_F1.8511.00
128_E132_E1.8281.00
101_E105_E1.8231.00
105_E108_I1.7851.00
7_G83_I1.7491.00
68_Q71_K1.7061.00
98_D102_K1.7051.00
102_K105_E1.6871.00
109_E112_E1.6651.00
55_Y72_V1.6621.00
65_S68_Q1.6531.00
98_D101_E1.6521.00
90_I94_F1.6371.00
52_I166_L1.6271.00
106_A109_E1.6231.00
40_L43_L1.6181.00
106_A110_K1.6161.00
52_I162_S1.5931.00
126_M130_Q1.5391.00
104_I108_I1.5281.00
109_E113_A1.5091.00
69_A163_A1.5001.00
130_Q133_E1.4801.00
70_F156_L1.4641.00
158_I162_S1.4551.00
49_F53_K1.4461.00
38_L41_F1.4451.00
129_E133_E1.4411.00
110_K113_A1.4331.00
4_L8_L1.4311.00
66_F70_F1.4151.00
107_A111_M1.4011.00
77_S81_S1.3691.00
125_G128_E1.3471.00
31_P35_Y1.3471.00
140_P143_N1.3241.00
6_L47_L1.3131.00
77_S159_S1.3081.00
117_S120_E1.2951.00
125_G129_E1.2941.00
107_A110_K1.2791.00
121_E124_I1.2671.00
121_E125_G1.2651.00
127_T131_I1.2611.00
103_Y107_A1.2351.00
17_I20_F1.2211.00
31_P34_G1.2021.00
101_E104_I1.1971.00
53_K166_L1.1891.00
162_S166_L1.1891.00
52_I73_G1.1861.00
87_F91_Y1.1741.00
133_E137_M1.1741.00
118_A121_E1.1711.00
66_F160_L1.1671.00
50_F54_S1.1541.00
89_Y94_F1.1461.00
14_M87_F1.1451.00
85_V144_A1.1391.00
38_L42_L1.1251.00
124_I127_T1.1161.00
3_G7_G1.0961.00
91_Y99_F1.0941.00
39_L42_L1.0881.00
133_E136_E1.0861.00
132_E136_E1.0851.00
136_E139_K1.0651.00
128_E131_I1.0611.00
123_A126_M1.0541.00
56_R168_K1.0541.00
111_M114_S1.0501.00
135_M139_K1.0331.00
148_F152_L1.0321.00
33_L37_S1.0261.00
146_M150_E1.0221.00
7_G11_I1.0221.00
123_A127_T1.0191.00
18_L21_L1.0171.00
34_G37_S1.0151.00
17_I21_L1.0071.00
72_V76_I1.0061.00
55_Y60_L1.0031.00
124_I128_E0.9991.00
54_S57_D0.9911.00
15_L87_F0.9861.00
89_Y144_A0.9861.00
104_I107_A0.9691.00
147_T151_S0.9621.00
3_G79_I0.9581.00
49_F162_S0.9491.00
2_Y54_S0.9461.00
82_L148_F0.9401.00
102_K106_A0.9391.00
131_I134_A0.9371.00
131_I135_M0.9241.00
111_M115_G0.9231.00
140_P144_A0.9121.00
60_L64_I0.9101.00
53_K56_R0.9081.00
19_Y22_G0.9061.00
126_M129_E0.9051.00
51_G72_V0.9011.00
150_E154_V0.9001.00
21_L24_H0.8971.00
122_E125_G0.8911.00
85_V148_F0.8911.00
146_M149_I0.8821.00
67_G71_K0.8801.00
10_S80_A0.8791.00
134_A138_M0.8781.00
14_M84_Y0.8771.00
100_M104_I0.8731.00
2_Y51_G0.8711.00
132_E135_M0.8691.00
115_G118_A0.8471.00
10_S84_Y0.8451.00
81_S151_S0.8441.00
18_L23_L0.8401.00
81_S85_V0.8391.00
89_Y92_F0.8221.00
6_L10_S0.8211.00
96_D102_K0.8211.00
71_K74_L0.8171.00
48_I52_I0.8141.00
71_K75_L0.8091.00
110_K114_S0.8091.00
4_L83_I0.8031.00
13_L18_L0.7961.00
121_E127_T0.7931.00
81_S155_G0.7921.00
74_L156_L0.7911.00
124_I131_I0.7811.00
84_Y88_W0.7771.00
5_I9_I0.7681.00
66_F164_A0.7651.00
128_E135_M0.7651.00
48_I77_S0.7651.00
116_A120_E0.7631.00
108_I111_M0.7611.00
134_A137_M0.7611.00
64_I166_L0.7591.00
20_F23_L0.7561.00
81_S148_F0.7441.00
17_I22_G0.7361.00
142_F146_M0.7331.00
50_F53_K0.7321.00
19_Y23_L0.7311.00
27_F106_A0.7301.00
88_W92_F0.7291.00
91_Y94_F0.7251.00
135_M138_M0.7191.00
122_E126_M0.7091.00
103_Y106_A0.7041.00
99_F103_Y0.7011.00
70_F163_A0.6991.00
39_L43_L0.6891.00
78_L152_L0.6851.00
91_Y95_I0.6851.00
10_S14_M0.6851.00
32_L36_L0.6841.00
164_A167_K0.6831.00
48_I162_S0.6791.00
76_I80_A0.6761.00
48_I73_G0.6761.00
55_Y59_N0.6731.00
83_I87_F0.6731.00
8_L12_V0.6651.00
52_I72_V0.6641.00
60_L63_F0.6631.00
60_L68_Q0.6621.00
100_M103_Y0.6601.00
17_I23_L0.6581.00
57_D62_G0.6581.00
142_F145_L0.6561.00
66_F163_A0.6541.00
2_Y72_V0.6511.00
70_F74_L0.6481.00
129_E132_E0.6461.00
67_G70_F0.6441.00
72_V75_L0.6421.00
151_S154_V0.6421.00
66_F165_I0.6351.00
11_I15_L0.6331.00
29_T32_L0.6331.00
49_F166_L0.6291.00
97_P101_E0.6281.00
74_L77_S0.6271.00
94_F97_P0.6271.00
155_G159_S0.6241.00
48_I51_G0.6141.00
56_R62_G0.6131.00
115_G119_A0.6121.00
28_D31_P0.6121.00
92_F99_F0.6031.00
11_I14_M0.6011.00
144_A148_F0.5981.00
116_A119_A0.5951.00
92_F100_M0.5931.00
7_G10_S0.5891.00
75_L79_I0.5891.00
38_L43_L0.5881.00
47_L51_G0.5851.00
10_S151_S0.5801.00
80_A84_Y0.5711.00
161_I164_A0.5681.00
63_F67_G0.5681.00
32_L37_S0.5641.00
154_V158_I0.5631.00
2_Y6_L0.5591.00
122_E127_T0.5591.00
29_T33_L0.5591.00
127_T135_M0.5541.00
128_E134_A0.5521.00
130_Q134_A0.5501.00
90_I95_I0.5491.00
156_L159_S0.5451.00
115_G120_E0.5391.00
30_G33_L0.5361.00
18_L24_H0.5331.00
52_I69_A0.5301.00
124_I134_A0.5281.00
75_L78_L0.5271.00
147_T150_E0.5251.00
84_Y151_S0.5231.00
16_L19_Y0.5221.00
79_I82_L0.5151.00
125_G131_I0.5111.00
125_G130_Q0.5091.00
9_I13_L0.5081.00
141_L145_L0.5021.00
2_Y5_I0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2l9uA 2 0.1845 8.1 0.907 Contact Map
1iijA 1 0.2024 5.2 0.915 Contact Map
4i0uA 4 0.3393 5.2 0.915 Contact Map
4ev6A 3 0.3512 3.8 0.921 Contact Map
3rvyA 3 0.5298 3.7 0.921 Contact Map
2l2tA 2 0.2143 3.5 0.921 Contact Map
4xp4A 1 0.9702 3.5 0.922 Contact Map
2m20A 2 0.1726 3.4 0.922 Contact Map
4av3A 2 0.5119 3.3 0.922 Contact Map
3j1zP 8 0.9464 3.2 0.923 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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