GREMLIN Database
DUF4197 - Protein of unknown function (DUF4197)
PFAM: PF13852 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 201 (200)
Sequences: 8903 (5526)
Seq/√Len: 390.7
META: 0.913

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
105_D109_Q3.8191.00
79_E122_A3.0461.00
33_L67_L3.0311.00
30_D34_G3.0041.00
112_K116_S2.4741.00
7_I97_K2.3611.00
26_L38_V2.2461.00
6_E198_K2.2401.00
168_K171_D2.1571.00
109_Q112_K2.1371.00
67_L71_R2.1301.00
35_N176_M2.1271.00
78_P82_P2.0661.00
180_E183_K2.0091.00
90_Q114_K1.9501.00
128_K162_D1.9201.00
104_D182_K1.9121.00
86_D118_Q1.9091.00
97_K101_K1.8971.00
75_D133_K1.8831.00
50_E54_R1.8771.00
128_K132_D1.8751.00
86_D90_Q1.8481.00
81_K85_V1.8331.00
26_L176_M1.8221.00
13_E16_E1.7981.00
75_D130_S1.7851.00
183_K187_N1.7171.00
117_A121_A1.7151.00
124_K163_D1.7121.00
120_T167_E1.7041.00
61_L64_E1.6741.00
120_T163_D1.6451.00
104_D186_K1.6151.00
44_E47_Q1.6141.00
37_A179_E1.6091.00
30_D71_R1.5831.00
20_E81_K1.5811.00
105_D182_K1.5571.00
58_M61_L1.5381.00
105_D178_A1.5311.00
60_K64_E1.5201.00
52_T55_K1.5141.00
194_D197_K1.5011.00
17_V21_N1.4791.00
79_E126_I1.4731.00
85_V89_K1.4511.00
32_F77_A1.4311.00
37_A41_P1.4221.00
109_Q113_R1.4011.00
175_L179_E1.3951.00
124_K128_K1.3881.00
183_K190_A1.3701.00
167_E171_D1.3631.00
94_Q98_G1.3571.00
80_A123_F1.3561.00
47_Q50_E1.3511.00
191_R196_L1.3461.00
179_E182_K1.3391.00
135_G138_K1.3291.00
79_E82_P1.3241.00
131_L136_A1.2961.00
50_E66_V1.2881.00
20_E24_A1.2821.00
160_D163_D1.2791.00
116_S120_T1.2671.00
124_K162_D1.2661.00
13_E193_T1.2541.00
182_K186_K1.2541.00
37_A175_L1.2531.00
82_P86_D1.2491.00
93_I96_A1.2471.00
3_S6_E1.2461.00
16_E85_V1.2451.00
163_D167_E1.2361.00
138_K142_D1.2351.00
97_K100_L1.2251.00
38_V172_G1.2021.00
54_R59_G1.1991.00
9_A195_L1.1981.00
39_K66_V1.1831.00
7_I100_L1.1581.00
71_R74_E1.1541.00
176_M179_E1.1521.00
103_G106_A1.1381.00
16_E89_K1.1371.00
118_Q121_A1.1371.00
96_A100_L1.1351.00
32_F169_A1.1231.00
52_T56_I1.1161.00
49_V52_T1.1111.00
54_R63_D1.0891.00
139_Y142_D1.0801.00
4_N8_A1.0781.00
59_G63_D1.0771.00
2_L10_G1.0661.00
144_A159_P1.0601.00
164_Y168_K1.0601.00
13_E195_L1.0591.00
6_E9_A1.0561.00
19_T22_A1.0531.00
191_R194_D1.0521.00
5_A9_A1.0431.00
50_E62_V1.0391.00
130_S134_V1.0361.00
30_D33_L1.0271.00
62_V143_L1.0221.00
16_E20_E1.0201.00
94_Q97_K1.0171.00
19_T84_F1.0141.00
65_L143_L1.0071.00
26_L77_A1.0061.00
132_D137_T0.9941.00
187_N190_A0.9921.00
47_Q51_K0.9881.00
75_D78_P0.9861.00
33_L66_V0.9851.00
68_S139_Y0.9791.00
78_P81_K0.9781.00
8_A96_A0.9761.00
42_L65_L0.9751.00
129_K133_K0.9741.00
51_K55_K0.9731.00
189_A192_T0.9621.00
7_I11_L0.9551.00
103_G109_Q0.9521.00
130_S133_K0.9331.00
2_L7_I0.9321.00
18_G177_I0.9311.00
25_K176_M0.9301.00
25_K28_K0.9261.00
17_V192_T0.9221.00
117_A120_T0.9161.00
45_E48_K0.9111.00
119_L170_L0.9061.00
141_N145_G0.9051.00
147_Y154_V0.9021.00
99_I107_A0.9021.00
158_N163_D0.9021.00
33_L39_K0.9011.00
116_S171_D0.8921.00
112_K171_D0.8911.00
35_N38_V0.8871.00
120_T170_L0.8851.00
4_N97_K0.8831.00
87_A114_K0.8831.00
49_V144_A0.8781.00
68_S134_V0.8781.00
64_E68_S0.8681.00
21_N24_A0.8641.00
27_G78_P0.8541.00
116_S170_L0.8471.00
146_K149_K0.8431.00
17_V193_T0.8431.00
44_E48_K0.8281.00
194_D198_K0.8281.00
129_K132_D0.8261.00
122_A126_I0.8251.00
69_M165_V0.8251.00
17_V20_E0.8211.00
83_I122_A0.8211.00
126_I129_K0.8161.00
25_K35_N0.8151.00
127_V166_T0.8121.00
193_T196_L0.8111.00
38_V176_M0.8031.00
53_L58_M0.7911.00
21_N180_E0.7861.00
34_G39_K0.7851.00
87_A115_T0.7851.00
98_G113_R0.7821.00
127_V165_V0.7781.00
131_L162_D0.7771.00
34_G71_R0.7681.00
120_T166_T0.7591.00
38_V175_L0.7521.00
65_L69_M0.7491.00
168_K175_L0.7451.00
141_N162_D0.7401.00
69_M131_L0.7391.00
192_T196_L0.7371.00
116_S167_E0.7371.00
36_P41_P0.7351.00
151_I154_V0.7341.00
180_E190_A0.7311.00
48_K52_T0.7311.00
32_F123_F0.7291.00
9_A13_E0.7281.00
11_L93_I0.7271.00
5_A8_A0.7211.00
32_F173_L0.7141.00
46_L140_Y0.7131.00
19_T23_V0.7111.00
142_D145_G0.7091.00
21_N25_K0.7081.00
21_N183_K0.7031.00
19_T88_I0.7001.00
63_D66_V0.6981.00
24_A81_K0.6961.00
75_D129_K0.6941.00
118_Q122_A0.6931.00
13_E196_L0.6920.99
61_L143_L0.6900.99
21_N192_T0.6860.99
53_L62_V0.6830.99
17_V196_L0.6730.99
65_L68_S0.6730.99
13_E17_V0.6730.99
42_L66_V0.6710.99
90_Q118_Q0.6700.99
77_A173_L0.6580.99
79_E129_K0.6490.99
88_I111_F0.6490.99
135_G139_Y0.6470.99
86_D89_K0.6460.99
23_V81_K0.6410.99
116_S174_F0.6410.99
64_E67_L0.6350.99
139_Y143_L0.6330.99
23_V77_A0.6310.99
8_A93_I0.6310.99
183_K186_K0.6310.99
16_E19_T0.6300.99
23_V26_L0.6270.99
120_T124_K0.6250.99
30_D67_L0.6230.99
68_S137_T0.6090.99
140_Y144_A0.6070.99
172_G176_M0.6060.99
105_D112_K0.6060.99
111_F116_S0.6030.99
188_P194_D0.6020.99
50_E53_L0.6020.99
146_K150_A0.6010.99
8_A12_K0.5980.99
162_D165_V0.5940.98
142_D146_K0.5920.98
189_A193_T0.5910.98
104_D109_Q0.5890.98
149_K152_P0.5890.98
50_E59_G0.5890.98
42_L69_M0.5890.98
53_L143_L0.5880.98
189_A194_D0.5880.98
93_I97_K0.5870.98
20_E85_V0.5840.98
4_N7_I0.5820.98
132_D141_N0.5820.98
136_A165_V0.5820.98
99_I110_Y0.5750.98
181_E185_R0.5730.98
131_L165_V0.5700.98
156_K159_P0.5700.98
7_I101_K0.5700.98
178_A182_K0.5670.98
8_A19_T0.5670.98
131_L137_T0.5660.98
136_A142_D0.5640.98
17_V177_I0.5630.98
80_A169_A0.5600.98
27_G77_A0.5590.98
121_A124_K0.5580.98
172_G175_L0.5570.98
131_L161_L0.5550.98
90_Q94_Q0.5510.98
84_F119_L0.5510.98
144_A157_V0.5500.97
10_G13_E0.5490.97
61_L139_Y0.5480.97
82_P85_V0.5460.97
33_L71_R0.5450.97
68_S136_A0.5440.97
29_T71_R0.5430.97
184_I191_R0.5410.97
62_V65_L0.5410.97
22_A177_I0.5400.97
83_I115_T0.5390.97
19_T111_F0.5380.97
4_N96_A0.5370.97
166_T169_A0.5350.97
124_K166_T0.5320.97
170_L175_L0.5310.97
94_Q114_K0.5310.97
62_V66_V0.5260.97
173_L176_M0.5240.97
175_L178_A0.5240.97
65_L137_T0.5210.97
41_P47_Q0.5210.97
19_T81_K0.5180.96
144_A147_Y0.5150.96
83_I118_Q0.5090.96
145_G159_P0.5080.96
90_Q110_Y0.5050.96
191_R197_K0.5040.96
54_R62_V0.5030.96
19_T177_I0.5020.96
145_G149_K0.5020.96
24_A28_K0.5010.96
180_E184_I0.5000.96
192_T197_K0.5000.96
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3lapA 6 0.5672 54.2 0.926 Contact Map
1b4aA 5 0.5771 31.8 0.935 Contact Map
3v4gA 5 0.607 19.8 0.941 Contact Map
1b4bA 4 0.3532 18.7 0.942 Contact Map
1xxaA 4 0.3582 16.5 0.943 Contact Map
4u6uB 1 0.7512 14.4 0.945 Contact Map
2zfzA 4 0.393 13 0.946 Contact Map
2w72B 1 0.6965 9.1 0.95 Contact Map
2p5mA 4 0.408 8.8 0.95 Contact Map
4o8aA 2 0.2189 8.8 0.95 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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