GREMLIN Database
DUF4158 - Domain of unknown function (DUF4158)
PFAM: PF13700 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 166 (162)
Sequences: 8867 (6382)
Seq/√Len: 501.4
META: 0.878

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
145_W149_R3.6051.00
34_L47_F3.1481.00
111_R117_D2.5541.00
114_T117_D2.5391.00
94_G98_R2.5131.00
27_L48_A2.4981.00
145_W148_E2.3691.00
95_R98_R2.2681.00
125_L142_A2.2241.00
90_Y100_H2.1581.00
38_R103_E2.1431.00
155_G158_T2.0841.00
49_L75_V2.0801.00
97_R101_R2.0471.00
54_F110_F2.0361.00
52_K55_R2.0061.00
157_S161_R1.9851.00
40_G43_N1.9311.00
105_R152_E1.8931.00
32_L36_R1.8401.00
120_A149_R1.8391.00
125_L138_L1.8381.00
121_L146_L1.8261.00
54_F152_E1.8051.00
121_L142_A1.7881.00
36_R44_R1.7711.00
34_L107_Y1.7691.00
124_W145_W1.7071.00
31_D55_R1.6861.00
143_I153_L1.6731.00
46_G100_H1.6501.00
35_I48_A1.5531.00
24_Y56_A1.5121.00
132_T137_D1.5111.00
31_D107_Y1.5071.00
95_R99_R1.4991.00
69_A73_A1.4941.00
113_F118_R1.4921.00
60_F101_R1.4921.00
141_A144_E1.4771.00
46_G90_Y1.4751.00
18_E22_A1.4271.00
17_S20_E1.4181.00
45_L72_V1.4081.00
27_L35_I1.3941.00
139_F163_V1.3911.00
13_L53_Y1.3901.00
8_T12_R1.3891.00
93_R99_R1.3811.00
54_F60_F1.3031.00
47_F104_I1.3011.00
45_L76_A1.2891.00
113_F117_D1.2611.00
51_L108_L1.2541.00
98_R101_R1.2471.00
122_A126_A1.2391.00
105_R112_P1.2321.00
98_R102_A1.2241.00
30_A33_A1.2121.00
128_A141_A1.2001.00
52_K75_V1.1851.00
29_D33_A1.1781.00
134_R137_D1.1701.00
135_P163_V1.1691.00
136_A140_N1.1481.00
47_F103_E1.1471.00
27_L52_K1.1411.00
34_L103_E1.1401.00
4_D7_R1.1361.00
49_L72_V1.1341.00
143_I147_R1.1271.00
144_E148_E1.1151.00
103_E106_E1.0961.00
119_R123_R1.0911.00
48_A79_L1.0821.00
48_A75_V1.0781.00
47_F107_Y1.0761.00
58_G147_R1.0701.00
4_D8_T1.0691.00
156_Y160_E1.0621.00
25_F71_V1.0591.00
122_A165_S1.0451.00
118_R158_T1.0361.00
110_F150_R1.0331.00
123_R127_E1.0321.00
7_R160_E1.0231.00
139_F159_L1.0171.00
50_Q100_H1.0151.00
26_T74_Y1.0061.00
67_V71_V1.0041.00
38_R47_F1.0001.00
27_L78_Q0.9941.00
142_A159_L0.9781.00
139_F143_I0.9731.00
25_F56_A0.9711.00
118_R122_A0.9681.00
49_L67_V0.9601.00
45_L75_V0.9511.00
50_Q61_L0.9451.00
115_A118_R0.9361.00
31_D51_L0.9281.00
63_D66_E0.9271.00
89_D92_W0.9251.00
146_L159_L0.9191.00
5_A9_A0.9161.00
83_D86_A0.9081.00
102_A105_R0.9041.00
47_F51_L0.9041.00
25_F52_K0.8981.00
160_E164_A0.8961.00
96_T99_R0.8931.00
124_W141_A0.8871.00
16_F20_E0.8801.00
21_L71_V0.8781.00
50_Q104_I0.8691.00
157_S160_E0.8671.00
101_R105_R0.8651.00
70_A73_A0.8381.00
49_L87_L0.8371.00
127_E130_L0.8291.00
5_A8_T0.8231.00
128_A131_T0.8181.00
22_A26_T0.8081.00
19_E23_R0.8051.00
56_A71_V0.8001.00
53_Y67_V0.7951.00
73_A77_R0.7911.00
103_E107_Y0.7811.00
121_L125_L0.7731.00
142_A146_L0.7681.00
120_A145_W0.7661.00
122_A162_L0.7631.00
51_L104_I0.7621.00
53_Y57_L0.7591.00
13_L21_L0.7481.00
138_L141_A0.7451.00
41_D44_R0.7391.00
143_I156_Y0.7331.00
125_L129_A0.7321.00
28_S31_D0.7311.00
141_A145_W0.7281.00
102_A106_E0.7271.00
125_L163_V0.7231.00
95_R102_A0.7161.00
111_R114_T0.7131.00
124_W142_A0.7071.00
54_F104_I0.7021.00
121_L124_W0.7011.00
37_R107_Y0.7011.00
117_D151_I0.6991.00
93_R96_T0.6981.00
86_A89_D0.6961.00
16_F70_A0.6951.00
45_L81_V0.6911.00
43_N100_H0.6911.00
140_N144_E0.6891.00
93_R98_R0.6871.00
8_T11_P0.6801.00
50_Q101_R0.6791.00
87_L90_Y0.6731.00
110_F147_R0.6721.00
18_E21_L0.6711.00
158_T161_R0.6711.00
124_W128_A0.6701.00
138_L142_A0.6631.00
158_T162_L0.6631.00
139_F160_E0.6621.00
113_F151_I0.6611.00
136_A139_F0.6611.00
74_Y78_Q0.6601.00
27_L31_D0.6581.00
113_F146_L0.6541.00
121_L162_L0.6471.00
120_A124_W0.6471.00
25_F57_L0.6451.00
34_L37_R0.6441.00
30_A107_Y0.6421.00
35_I38_R0.6391.00
53_Y59_R0.6361.00
42_H46_G0.6351.00
53_Y71_V0.6291.00
106_E109_G0.6261.00
123_R126_A0.6241.00
137_D140_N0.6241.00
132_T135_P0.6201.00
16_F21_L0.6161.00
27_L32_L0.6090.99
31_D52_K0.6080.99
50_Q53_Y0.6070.99
52_K56_A0.6040.99
81_V86_A0.6000.99
137_D153_L0.5940.99
28_S108_L0.5930.99
35_I44_R0.5900.99
19_E22_A0.5890.99
102_A112_P0.5880.99
116_A120_A0.5840.99
41_D81_V0.5830.99
4_D9_A0.5810.99
94_G101_R0.5750.99
142_A145_W0.5750.99
39_R43_N0.5750.99
137_D141_A0.5700.99
52_K71_V0.5640.99
161_R165_S0.5630.99
53_Y60_F0.5620.99
48_A78_Q0.5610.99
10_L13_L0.5600.99
116_A119_R0.5590.99
75_V79_L0.5590.99
85_A88_A0.5580.99
113_F158_T0.5560.99
46_G87_L0.5540.99
20_E56_A0.5530.99
135_P164_A0.5520.99
153_L159_L0.5510.99
50_Q54_F0.5510.99
13_L68_P0.5490.99
59_R153_L0.5450.99
53_Y56_A0.5400.99
32_L78_Q0.5370.99
113_F154_P0.5360.99
96_T100_H0.5340.99
64_P97_R0.5330.99
110_F152_E0.5330.99
13_L16_F0.5300.99
33_A37_R0.5280.99
119_R122_A0.5260.99
44_R48_A0.5200.98
34_L38_R0.5190.98
10_L153_L0.5180.98
53_Y61_L0.5180.98
88_A91_A0.5150.98
140_N143_I0.5120.98
46_G50_Q0.5080.98
144_E147_R0.5050.98
32_L35_I0.5050.98
85_A89_D0.5030.98
16_F56_A0.5030.98
60_F104_I0.5020.98
83_D88_A0.5010.98
86_A90_Y0.5000.98
133_D137_D0.5000.98
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4fcyA 4 0.741 10.3 0.931 Contact Map
1xl7A 1 0.8554 5.9 0.938 Contact Map
1dp7P 1 0.3012 5.9 0.938 Contact Map
1xszA 1 0.6747 5.4 0.939 Contact Map
2o4dA 3 0.4157 5.3 0.939 Contact Map
1pdnC 1 0.6024 5.1 0.94 Contact Map
1nm8A 1 0.8614 4.6 0.941 Contact Map
1pm6A 1 0.1687 4.2 0.942 Contact Map
4n78F 1 0.7771 4.1 0.942 Contact Map
3i3nA 2 0.6446 4.1 0.942 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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