GREMLIN Database
EcoRI_methylase - Adenine-specific methyltransferase EcoRI
PFAM: PF13651 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 352 (347)
Sequences: 3369 (2538)
Seq/√Len: 136.3
META: 0.929

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
286_V348_L4.6321.00
243_D248_H4.2351.00
162_V186_I3.6141.00
207_E222_R2.9671.00
24_L173_L2.9121.00
309_K342_R2.8941.00
255_K261_E2.7661.00
284_D351_N2.6381.00
307_L345_A2.6111.00
258_N261_E2.5631.00
332_K335_Y2.5081.00
55_N58_K2.4011.00
331_D336_P2.3451.00
162_V174_I2.3271.00
209_I228_K2.3091.00
109_D113_D2.2861.00
105_D109_D2.2111.00
335_Y342_R2.0631.00
224_D228_K2.0341.00
199_Y244_H2.0211.00
178_Q292_T2.0021.00
51_Y59_L1.9901.00
330_G334_G1.9821.00
333_G336_P1.9231.00
331_D334_G1.9221.00
345_A348_L1.9141.00
335_Y345_A1.9121.00
166_V189_L1.8841.00
141_E144_K1.8821.00
334_G337_Y1.8541.00
32_R60_G1.8091.00
50_K54_L1.7981.00
264_K269_D1.7951.00
328_D331_D1.7891.00
334_G342_R1.7851.00
207_E230_Y1.7751.00
205_D232_R1.7411.00
329_G332_K1.7371.00
331_D335_Y1.7111.00
329_G334_G1.7061.00
326_K330_G1.6831.00
231_I234_G1.6821.00
317_G321_G1.6711.00
63_K99_E1.6541.00
329_G333_G1.6281.00
259_P263_P1.6261.00
330_G333_G1.6201.00
332_K336_P1.6191.00
342_R345_A1.6111.00
186_I195_L1.6041.00
233_V236_V1.6031.00
201_F206_M1.5981.00
337_Y342_R1.5931.00
308_T342_R1.5821.00
163_A167_E1.5661.00
313_T317_G1.5661.00
333_G342_R1.5631.00
325_Y329_G1.5591.00
330_G336_P1.5581.00
310_G342_R1.5451.00
200_G206_M1.5341.00
307_L310_G1.5281.00
332_K342_R1.5221.00
201_F205_D1.5181.00
317_G320_L1.5141.00
308_T345_A1.5081.00
303_E350_R1.5051.00
323_K326_K1.5011.00
337_Y340_G1.4941.00
321_G324_G1.4871.00
321_G326_K1.4811.00
326_K331_D1.4731.00
202_N205_D1.4681.00
206_M236_V1.4671.00
206_M234_G1.4541.00
317_G324_G1.4521.00
326_K335_Y1.4351.00
207_E221_R1.4281.00
335_Y338_I1.4191.00
328_D335_Y1.4121.00
325_Y331_D1.4081.00
329_G335_Y1.4031.00
207_E232_R1.4021.00
315_V318_Y1.3871.00
328_D333_G1.3821.00
336_P343_P1.3781.00
336_P342_R1.3711.00
330_G335_Y1.3591.00
325_Y333_G1.3571.00
325_Y335_Y1.3471.00
230_Y234_G1.3461.00
205_D233_V1.3341.00
329_G336_P1.3341.00
325_Y342_R1.3331.00
326_K332_K1.3281.00
314_G317_G1.3241.00
18_S21_E1.3231.00
205_D234_G1.3161.00
207_E234_G1.3131.00
331_D342_R1.3101.00
307_L342_R1.3081.00
316_K319_K1.3071.00
201_F204_G1.2941.00
180_A183_Y1.2901.00
326_K333_G1.2891.00
216_L231_I1.2891.00
333_G337_Y1.2761.00
318_Y321_G1.2741.00
326_K342_R1.2731.00
201_F207_E1.2731.00
206_M233_V1.2651.00
323_K327_K1.2361.00
315_V319_K1.2301.00
70_K131_D1.2251.00
320_L342_R1.2231.00
328_D334_G1.2151.00
216_L221_R1.2131.00
316_K320_L1.2101.00
319_K323_K1.2051.00
328_D336_P1.1951.00
293_F297_Y1.1951.00
333_G345_A1.1931.00
306_G309_K1.1881.00
182_T345_A1.1790.99
321_G325_Y1.1780.99
218_A234_G1.1770.99
232_R235_N1.1750.99
137_D141_E1.1750.99
105_D116_D1.1670.99
317_G323_K1.1620.99
329_G342_R1.1590.99
322_K325_Y1.1580.99
209_I230_Y1.1510.99
313_T316_K1.1510.99
311_K314_G1.1490.99
336_P340_G1.1490.99
218_A231_I1.1480.99
313_T321_G1.1480.99
327_K335_Y1.1440.99
94_K127_Y1.1410.99
313_T320_L1.1400.99
317_G325_Y1.1340.99
309_K345_A1.1310.99
314_G318_Y1.1300.99
314_G319_K1.1270.99
107_N113_D1.1240.99
330_G337_Y1.1240.99
325_Y332_K1.1240.99
248_H300_E1.1140.99
50_K53_A1.1120.99
318_Y323_K1.1120.99
200_G205_D1.1080.99
220_G232_R1.1050.99
328_D332_K1.1050.99
210_V214_Y1.1030.99
308_T343_P1.0980.99
109_D116_D1.0970.99
317_G334_G1.0950.99
258_N263_P1.0940.99
326_K329_G1.0910.99
275_K278_D1.0840.99
319_K325_Y1.0820.99
327_K342_R1.0820.99
200_G236_V1.0750.99
324_G328_D1.0750.99
203_S207_E1.0750.99
331_D337_Y1.0710.99
221_R224_D1.0700.99
201_F237_C1.0690.99
216_L234_G1.0660.99
207_E231_I1.0610.99
199_Y202_N1.0610.99
308_T335_Y1.0550.99
206_M237_C1.0550.99
318_Y342_R1.0490.99
286_V305_I1.0450.99
313_T318_Y1.0420.99
324_G335_Y1.0380.99
212_D229_K1.0380.99
202_N207_E1.0350.99
207_E237_C1.0300.99
218_A221_R1.0270.99
212_D228_K1.0200.98
324_G327_K1.0190.98
320_L323_K1.0160.98
318_Y322_K1.0120.98
324_G334_G1.0050.98
67_T128_L1.0030.98
336_P345_A1.0010.98
190_I294_L1.0010.98
308_T334_G1.0010.98
334_G345_A0.9950.98
310_G345_A0.9910.98
327_K331_D0.9910.98
217_Y221_R0.9880.98
140_I144_K0.9840.98
309_K334_G0.9770.98
30_H242_L0.9770.98
314_G321_G0.9740.98
201_F234_G0.9730.98
307_L348_L0.9730.98
306_G310_G0.9720.98
325_Y334_G0.9690.98
205_D236_V0.9680.98
105_D113_D0.9670.98
326_K336_P0.9610.98
110_G116_D0.9600.98
332_K345_A0.9510.98
323_K342_R0.9490.98
318_Y324_G0.9490.98
322_K326_K0.9470.98
173_L242_L0.9450.98
310_G333_G0.9450.98
310_G334_G0.9440.97
316_K321_G0.9440.97
324_G342_R0.9410.97
334_G343_P0.9410.97
28_K58_K0.9400.97
178_Q202_N0.9370.97
204_G207_E0.9370.97
207_E233_V0.9360.97
222_R232_R0.9310.97
216_L223_I0.9300.97
219_T231_I0.9270.97
326_K334_G0.9220.97
255_K281_S0.9210.97
323_K335_Y0.9200.97
232_R236_V0.9200.97
196_W247_R0.9190.97
207_E216_L0.9190.97
326_K345_A0.9100.97
334_G338_I0.9100.97
202_N237_C0.9080.97
202_N232_R0.9080.97
204_G236_V0.9080.97
135_R160_E0.9070.97
178_Q237_C0.9030.97
219_T224_D0.8980.97
309_K336_P0.8970.97
200_G203_S0.8930.96
201_F231_I0.8860.96
325_Y328_D0.8850.96
203_S237_C0.8840.96
318_Y325_Y0.8800.96
203_S206_M0.8790.96
315_V324_G0.8760.96
216_L220_G0.8750.96
232_R237_C0.8750.96
217_Y231_I0.8740.96
250_P298_N0.8730.96
4_K7_K0.8720.96
206_M231_I0.8720.96
218_A222_R0.8710.96
36_V61_L0.8700.96
268_Y286_V0.8690.96
309_K343_P0.8690.96
36_V150_V0.8680.96
329_G337_Y0.8680.96
3_H6_K0.8670.96
308_T317_G0.8670.96
312_R317_G0.8660.96
323_K333_G0.8630.96
21_E51_Y0.8620.96
327_K333_G0.8620.96
257_Y277_D0.8610.96
320_L326_K0.8560.96
323_K334_G0.8540.95
63_K145_E0.8530.95
201_F229_K0.8500.95
307_L335_Y0.8490.95
337_Y345_A0.8460.95
327_K330_G0.8440.95
220_G231_I0.8430.95
317_G342_R0.8400.95
178_Q197_L0.8360.95
18_S22_K0.8340.95
207_E228_K0.8320.95
178_Q234_G0.8320.95
324_G333_G0.8310.95
140_I168_Y0.8310.95
318_Y334_G0.8290.95
317_G326_K0.8260.95
343_P348_L0.8260.95
319_K335_Y0.8240.95
200_G237_C0.8230.94
228_K231_I0.8210.94
216_L230_Y0.8200.94
182_T291_I0.8200.94
308_T332_K0.8190.94
201_F236_V0.8180.94
214_Y231_I0.8180.94
224_D230_Y0.8170.94
315_V320_L0.8150.94
321_G330_G0.8140.94
221_R231_I0.8120.94
324_G332_K0.8110.94
207_E220_G0.8040.94
313_T323_K0.8030.94
320_L324_G0.8030.94
178_Q201_F0.8000.94
309_K335_Y0.7980.94
335_Y339_N0.7970.93
229_K234_G0.7920.93
178_Q232_R0.7900.93
325_Y330_G0.7880.93
314_G323_K0.7860.93
205_D218_A0.7860.93
31_F150_V0.7860.93
327_K336_P0.7830.93
318_Y335_Y0.7790.93
335_Y340_G0.7790.93
210_V229_K0.7770.93
220_G234_G0.7760.93
144_K168_Y0.7750.93
165_L168_Y0.7750.93
308_T333_G0.7710.92
215_E218_A0.7710.92
327_K334_G0.7700.92
308_T336_P0.7680.92
216_L219_T0.7650.92
332_K337_Y0.7650.92
37_L139_C0.7640.92
197_L202_N0.7630.92
181_I197_L0.7610.92
312_R316_K0.7610.92
221_R229_K0.7600.92
321_G333_G0.7590.92
328_D342_R0.7570.92
327_K332_K0.7530.91
210_V213_D0.7530.91
17_L21_E0.7530.91
205_D237_C0.7520.91
325_Y336_P0.7480.91
205_D231_I0.7470.91
207_E236_V0.7440.91
332_K340_G0.7420.91
205_D230_Y0.7410.91
222_R228_K0.7400.91
317_G322_K0.7380.91
221_R234_G0.7380.91
313_T319_K0.7340.90
317_G332_K0.7320.90
218_A232_R0.7320.90
335_Y343_P0.7300.90
330_G342_R0.7290.90
318_Y330_G0.7240.90
29_D32_R0.7210.90
332_K338_I0.7210.90
321_G335_Y0.7170.89
161_Y164_Q0.7160.89
231_I235_N0.7130.89
86_L89_D0.7110.89
323_K330_G0.7110.89
310_G330_G0.7110.89
323_K332_K0.7100.89
331_D345_A0.7100.89
315_V321_G0.7090.89
308_T331_D0.7080.89
200_G207_E0.7070.89
337_Y343_P0.7060.89
318_Y326_K0.7010.88
215_E222_R0.7000.88
70_K95_A0.7000.88
222_R231_I0.6970.88
234_G237_C0.6970.88
76_G80_S0.6970.88
316_K342_R0.6960.88
217_Y222_R0.6950.88
338_I341_K0.6930.88
323_K345_A0.6920.88
314_G320_L0.6900.87
324_G329_G0.6890.87
215_E219_T0.6880.87
322_K335_Y0.6850.87
97_K100_I0.6850.87
319_K324_G0.6830.87
323_K329_G0.6830.87
200_G232_R0.6790.87
321_G342_R0.6790.87
220_G223_I0.6750.86
317_G333_G0.6740.86
264_K283_Y0.6730.86
206_M229_K0.6660.86
171_K241_N0.6660.86
319_K322_K0.6630.85
328_D337_Y0.6620.85
206_M220_G0.6620.85
205_D221_R0.6620.85
219_T223_I0.6560.85
203_S216_L0.6560.85
318_Y327_K0.6550.85
332_K343_P0.6540.84
200_G231_I0.6510.84
333_G338_I0.6490.84
320_L325_Y0.6430.83
311_K316_K0.6430.83
140_I164_Q0.6430.83
315_V326_K0.6390.83
218_A223_I0.6390.83
337_Y348_L0.6370.83
199_Y246_K0.6370.83
222_R230_Y0.6370.83
309_K317_G0.6360.83
200_G204_G0.6350.83
206_M232_R0.6330.83
324_G330_G0.6330.83
207_E218_A0.6310.82
216_L229_K0.6310.82
321_G327_K0.6310.82
201_F218_A0.6300.82
313_T324_G0.6290.82
309_K333_G0.6290.82
112_I116_D0.6280.82
316_K322_K0.6260.82
202_N234_G0.6260.82
321_G328_D0.6260.82
324_G331_D0.6260.82
193_N297_Y0.6240.82
105_D110_G0.6230.82
336_P339_N0.6220.81
201_F222_R0.6190.81
311_K317_G0.6170.81
204_G233_V0.6150.81
201_F233_V0.6150.81
312_R318_Y0.6140.81
319_K327_K0.6110.80
325_Y345_A0.6110.80
207_E235_N0.6070.80
201_F232_R0.6070.80
322_K342_R0.6060.80
221_R230_Y0.6060.80
206_M219_T0.6060.80
309_K332_K0.6050.80
323_K328_D0.6040.80
256_K281_S0.6040.80
199_Y230_Y0.6020.79
317_G328_D0.6020.79
200_G233_V0.6000.79
216_L222_R0.5980.79
310_G329_G0.5970.79
202_N220_G0.5960.79
311_K334_G0.5940.78
166_V169_D0.5910.78
200_G234_G0.5910.78
24_L36_V0.5890.78
326_K337_Y0.5890.78
310_G321_G0.5880.78
204_G234_G0.5870.78
309_K316_K0.5870.78
257_Y263_P0.5850.77
202_N231_I0.5840.77
137_D140_I0.5820.77
265_Y272_E0.5760.76
312_R321_G0.5760.76
331_D343_P0.5750.76
219_T234_G0.5740.76
320_L329_G0.5710.76
317_G329_G0.5710.76
223_I228_K0.5640.75
209_I234_G0.5630.75
310_G335_Y0.5630.75
172_F195_L0.5630.75
205_D210_V0.5620.75
318_Y333_G0.5610.74
203_S220_G0.5610.74
202_N206_M0.5600.74
309_K326_K0.5600.74
225_E228_K0.5590.74
219_T232_R0.5570.74
308_T337_Y0.5520.73
219_T222_R0.5510.73
206_M235_N0.5500.73
158_F180_A0.5500.73
16_Q19_D0.5500.73
215_E234_G0.5490.73
314_G322_K0.5490.73
319_K336_P0.5470.73
223_I226_N0.5430.72
311_K315_V0.5410.72
117_I120_L0.5370.71
305_I308_T0.5370.71
204_G232_R0.5360.71
316_K323_K0.5340.71
316_K333_G0.5330.71
77_T80_S0.5330.71
317_G336_P0.5290.70
230_Y233_V0.5290.70
220_G224_D0.5270.70
222_R234_G0.5260.70
320_L330_G0.5250.69
314_G342_R0.5250.69
306_G335_Y0.5240.69
338_I343_P0.5240.69
316_K335_Y0.5240.69
204_G237_C0.5210.69
319_K333_G0.5210.69
313_T326_K0.5210.69
312_R320_L0.5190.69
315_V325_Y0.5170.68
328_D345_A0.5170.68
228_K234_G0.5140.68
311_K335_Y0.5140.68
201_F216_L0.5130.68
308_T311_K0.5130.68
318_Y331_D0.5130.68
203_S231_I0.5120.68
316_K330_G0.5120.68
213_D229_K0.5120.68
320_L335_Y0.5110.67
309_K318_Y0.5100.67
209_I222_R0.5090.67
312_R319_K0.5090.67
334_G348_L0.5060.67
217_Y220_G0.5060.67
308_T324_G0.5050.67
243_D247_R0.5050.67
70_K93_E0.5050.67
207_E219_T0.5030.66
310_G317_G0.5020.66
312_R315_V0.5000.66
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2f8lA 1 0.5994 51 0.974 Contact Map
2okcA 1 0.7188 36 0.977 Contact Map
2kz2A 1 0.2642 25.9 0.978 Contact Map
3ufbA 1 0.6932 25.9 0.978 Contact Map
2m1uA 1 0.2614 14 0.981 Contact Map
4bzjA 2 0 12.1 0.981 Contact Map
2m8uA 1 0.2188 9.5 0.982 Contact Map
2ml9A 1 0.1648 9.4 0.982 Contact Map
2b1uA 1 0.1989 9.2 0.982 Contact Map
4g3bA 3 0.0653 8.8 0.982 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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