GREMLIN Database
DUF4133 - Domain of unknown function (DUF4133)
PFAM: PF13571 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 91 (90)
Sequences: 1489 (957)
Seq/√Len: 100.9
META: 0.868

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
25_G57_L4.4581.00
14_L62_F3.9511.00
60_Q63_R3.0581.00
29_L50_I2.7541.00
55_T59_W2.3631.00
20_W24_G2.2711.00
16_A70_E2.1171.00
63_R67_K2.0591.00
51_L54_G1.9511.00
22_L58_F1.9171.00
43_Q47_L1.8371.00
42_N48_G1.8371.00
68_Y76_K1.7131.00
56_G60_Q1.7071.00
25_G61_V1.6471.00
49_L53_L1.6191.00
45_I49_L1.5181.00
10_E15_K1.5151.00
85_Y89_R1.4721.00
51_L55_T1.4661.00
48_G52_G1.4561.00
44_Y48_G1.3861.00
52_G66_R1.3730.99
77_L81_S1.3600.99
42_N67_K1.3420.99
36_L41_V1.3180.99
30_L34_A1.3090.99
22_L61_V1.2910.99
60_Q73_L1.2600.99
58_F61_V1.2220.99
29_L54_G1.2130.99
8_P70_E1.1790.98
81_S88_S1.1440.98
33_F37_Y1.1160.98
34_A38_I1.1120.98
7_K10_E1.0960.97
52_G56_G1.0940.97
76_K80_R1.0850.97
84_R89_R1.0850.97
36_L46_C1.0760.97
82_H85_Y1.0710.97
2_N6_N1.0700.97
42_N51_L1.0510.97
22_L25_G1.0460.97
9_I19_I1.0300.96
5_I85_Y1.0070.96
20_W23_A0.9770.95
29_L57_L0.9760.95
19_I22_L0.9620.95
6_N65_S0.9520.94
19_I33_F0.9280.93
32_L36_L0.9270.93
30_L71_H0.9250.93
16_A65_S0.9190.93
69_G72_G0.9060.93
32_L86_I0.8850.92
24_G28_A0.8810.91
87_K90_S0.8610.91
43_Q58_F0.8590.90
64_L67_K0.8580.90
65_S90_S0.8400.89
44_Y52_G0.8360.89
15_K19_I0.8350.89
39_A47_L0.8220.88
85_Y88_S0.8150.88
53_L56_G0.8130.88
73_L77_L0.7930.87
71_H74_M0.7830.86
60_Q77_L0.7700.85
47_L81_S0.7650.85
37_Y46_C0.7550.84
26_L58_F0.7540.84
21_Y73_L0.7470.83
29_L53_L0.7390.82
23_A85_Y0.7380.82
20_W70_E0.7290.82
2_N5_I0.7270.81
45_I55_T0.7260.81
79_K85_Y0.7160.80
11_F75_K0.7060.80
28_A84_R0.6980.79
5_I29_L0.6960.79
14_L58_F0.6950.78
28_A57_L0.6930.78
14_L20_W0.6850.77
82_H90_S0.6780.77
67_K76_K0.6720.76
5_I37_Y0.6660.75
39_A85_Y0.6650.75
33_F46_C0.6630.75
35_I39_A0.6610.75
9_I77_L0.6580.75
55_T66_R0.6550.74
31_I44_Y0.6530.74
66_R74_M0.6500.74
68_Y73_L0.6470.73
8_P15_K0.6380.72
28_A59_W0.6370.72
44_Y47_L0.6270.71
64_L73_L0.6250.71
46_C76_K0.6230.71
47_L51_L0.6220.70
77_L82_H0.6160.70
59_W66_R0.6150.70
39_A66_R0.6020.68
66_R81_S0.6000.68
25_G86_I0.5960.67
43_Q76_K0.5950.67
44_Y66_R0.5930.67
52_G87_K0.5900.66
85_Y90_S0.5840.66
28_A77_L0.5820.65
64_L76_K0.5790.65
3_K7_K0.5690.64
26_L38_I0.5680.63
54_G88_S0.5630.63
53_L81_S0.5630.63
65_S75_K0.5530.61
6_N21_Y0.5520.61
14_L75_K0.5480.61
71_H89_R0.5390.59
17_Q37_Y0.5370.59
15_K66_R0.5350.59
59_W77_L0.5340.59
5_I17_Q0.5300.58
5_I8_P0.5200.57
18_Y75_K0.5190.57
9_I90_S0.5170.56
8_P33_F0.5130.56
55_T84_R0.5100.55
1_I37_Y0.5100.55
49_L80_R0.5070.55
36_L69_G0.5060.55
31_I35_I0.5050.55
43_Q81_S0.5000.54
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4ci2B 1 0.2308 3.9 0.916 Contact Map
3l1lA 2 0.7363 2.6 0.923 Contact Map
4pypA 1 0.6923 2.3 0.926 Contact Map
4q4hB 1 0.967 2.2 0.926 Contact Map
3h9nA 3 0.3846 2.2 0.927 Contact Map
3b5xA 2 0.967 2.1 0.927 Contact Map
3b9wA 3 0.8352 2.1 0.928 Contact Map
1pp5A 1 0.1429 2.1 0.928 Contact Map
4a82A 2 0.967 2 0.928 Contact Map
4q4hA 1 0.9451 2 0.928 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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