GREMLIN Database
DUF4125 - Protein of unknown function (DUF4125)
PFAM: PF13526 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 197 (195)
Sequences: 918 (750)
Seq/√Len: 53.7
META: 0.818

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
173_R178_E4.0391.00
65_R70_E3.7061.00
119_K177_E3.1311.00
24_R29_D2.8511.00
80_A83_E2.7971.00
52_S157_T2.5821.00
13_E61_K2.5631.00
49_T164_D2.4381.00
102_I151_E2.4131.00
14_M67_L2.2701.00
46_S49_T2.2431.00
30_D33_T2.2261.00
85_E88_K2.1511.00
74_R84_Y2.1501.00
190_A194_E2.1061.00
56_D157_T2.0671.00
52_S56_D2.0481.00
60_A67_L2.0091.00
184_V195_A1.9701.00
167_E171_E1.9681.00
46_S164_D1.9631.00
97_E155_D1.9461.00
168_L175_L1.9351.00
113_Q176_A1.8990.99
114_E131_H1.8930.99
181_E185_K1.8640.99
77_E99_K1.8410.99
73_A87_I1.8170.99
20_N23_G1.8070.99
162_Y166_L1.7950.99
74_R151_E1.7860.99
55_D156_K1.7820.99
31_P35_R1.7590.99
52_S160_L1.7580.99
12_W16_Q1.7420.99
129_P139_G1.7220.99
113_Q142_F1.6730.99
123_L195_A1.6680.99
95_S155_D1.6590.99
33_T187_Y1.6140.98
123_L184_V1.6070.98
146_L161_Y1.5900.98
132_S135_D1.5890.98
168_L178_E1.5590.98
10_L61_K1.5480.98
117_A130_L1.5460.98
48_E163_R1.5390.98
103_E147_R1.5190.97
104_E162_Y1.5060.97
112_W175_L1.4940.97
6_E58_E1.4780.97
25_A28_Q1.4720.97
115_E119_K1.4700.97
135_D144_T1.4550.97
21_E80_A1.4400.96
77_E88_K1.4380.96
3_L50_L1.4320.96
110_V130_L1.4120.96
112_W174_N1.3830.96
54_L58_E1.3750.95
177_E181_E1.3220.94
33_T37_M1.3190.94
83_E86_E1.2900.94
116_L180_L1.2880.93
56_D154_S1.2850.93
42_F53_Y1.2490.92
104_E166_L1.2260.92
12_W31_P1.2250.92
42_F45_W1.2180.91
5_E39_K1.2120.91
4_I7_I1.2030.91
102_I106_V1.1840.90
16_Q24_R1.1840.90
144_T148_G1.1710.90
60_A157_T1.1580.89
95_S98_K1.1490.89
141_S144_T1.1470.89
114_E118_E1.1470.89
168_L173_R1.1420.89
98_K102_I1.1340.88
18_V28_Q1.1260.88
94_L98_K1.1130.87
111_E169_K1.1090.87
105_I158_L1.1050.87
143_E147_R1.1040.87
156_K160_L1.1020.87
164_D168_L1.0930.86
15_F27_C1.0900.86
109_Q176_A1.0770.85
43_S186_L1.0440.84
74_R98_K1.0420.83
96_E100_P1.0390.83
116_L177_E1.0380.83
77_E84_Y1.0240.82
18_V68_L1.0110.82
103_E106_V1.0100.81
109_Q146_L1.0020.81
44_T179_I0.9990.81
37_M41_Q0.9960.81
15_F18_V0.9960.81
8_I35_R0.9940.80
67_L74_R0.9900.80
140_T144_T0.9890.80
20_N25_A0.9790.79
177_E192_L0.9690.79
108_I162_Y0.9620.78
106_V147_R0.9600.78
101_L159_E0.9600.78
53_Y153_Y0.9520.77
22_G25_A0.9440.77
108_I169_K0.9230.75
184_V189_Y0.9220.75
37_M40_S0.9200.75
104_E107_A0.9200.75
98_K155_D0.9080.74
41_Q141_S0.9060.74
52_S57_L0.8930.73
94_L151_E0.8930.73
84_Y88_K0.8910.73
18_V27_C0.8750.71
5_E9_E0.8720.71
178_E181_E0.8680.71
116_L124_A0.8670.71
83_E87_I0.8670.71
112_W195_A0.8590.70
43_S182_N0.8590.70
9_E31_P0.8590.70
117_A124_A0.8540.69
118_E167_E0.8510.69
74_R94_L0.8510.69
105_I165_I0.8480.69
42_F176_A0.8450.69
14_M98_K0.8440.69
175_L196_E0.8370.68
17_K68_L0.8310.67
71_K145_Y0.8300.67
27_C145_Y0.8180.66
36_I187_Y0.8160.66
34_F38_R0.8120.66
6_E54_L0.8030.65
113_Q124_A0.8030.65
21_E83_E0.8010.64
16_Q68_L0.7990.64
128_R145_Y0.7920.64
29_D32_E0.7830.63
8_I39_K0.7820.63
156_K159_E0.7820.63
120_Y193_E0.7810.62
135_D141_S0.7700.61
98_K151_E0.7630.61
49_T161_Y0.7620.61
57_L157_T0.7580.60
13_E17_K0.7580.60
41_Q161_Y0.7540.60
75_M145_Y0.7500.59
159_E163_R0.7490.59
16_Q42_F0.7450.59
109_Q113_Q0.7440.59
106_V162_Y0.7410.58
18_V33_T0.7410.58
16_Q29_D0.7400.58
41_Q179_I0.7380.58
52_S60_A0.7350.58
108_I111_E0.7310.57
73_A112_W0.7270.57
82_E85_E0.7270.57
21_E88_K0.7210.56
28_Q75_M0.7170.56
21_E148_G0.7140.56
175_L179_I0.7120.55
12_W157_T0.7100.55
44_T182_N0.7100.55
84_Y151_E0.7050.55
42_F57_L0.7010.54
7_I11_E0.7000.54
74_R176_A0.6980.54
10_L54_L0.6940.53
112_W123_L0.6940.53
177_E180_L0.6930.53
122_K196_E0.6910.53
115_E177_E0.6900.53
140_T148_G0.6900.53
189_Y194_E0.6900.53
30_D41_Q0.6880.53
164_D175_L0.6860.53
148_G160_L0.6850.53
161_Y165_I0.6770.52
72_Y145_Y0.6760.52
79_T84_Y0.6740.51
75_M128_R0.6720.51
76_M79_T0.6690.51
5_E35_R0.6670.51
109_Q142_F0.6610.50
120_Y177_E0.6590.50
45_W161_Y0.6580.50
20_N28_Q0.6570.50
166_L170_K0.6570.50
53_Y67_L0.6570.50
101_L162_Y0.6520.49
26_S32_E0.6490.49
129_P132_S0.6490.49
55_D59_E0.6480.49
70_E152_T0.6450.48
113_Q183_T0.6420.48
46_S98_K0.6420.48
177_E193_E0.6410.48
76_M145_Y0.6390.48
154_S157_T0.6370.47
40_S45_W0.6360.47
128_R140_T0.6350.47
42_F49_T0.6340.47
42_F109_Q0.6320.47
110_V143_E0.6310.47
59_E156_K0.6280.46
14_M102_I0.6280.46
51_E55_D0.6270.46
45_W49_T0.6250.46
109_Q161_Y0.6250.46
48_E51_E0.6250.46
52_S154_S0.6230.46
72_Y75_M0.6200.46
47_E51_E0.6200.46
112_W146_L0.6180.45
52_S156_K0.6160.45
27_C75_M0.6150.45
44_T178_E0.6110.45
12_W25_A0.6090.44
33_T40_S0.6050.44
180_L187_Y0.6040.44
33_T152_T0.6030.44
80_A85_E0.6010.44
45_W113_Q0.5970.43
37_M113_Q0.5930.43
185_K193_E0.5930.43
109_Q112_W0.5920.43
41_Q109_Q0.5900.42
142_F176_A0.5890.42
49_T160_L0.5870.42
39_K186_L0.5810.42
64_G71_K0.5800.41
69_T90_L0.5790.41
67_L153_Y0.5790.41
10_L13_E0.5770.41
39_K43_S0.5760.41
53_Y57_L0.5740.41
110_V141_S0.5660.40
12_W32_E0.5640.40
155_D159_E0.5590.39
160_L164_D0.5560.39
103_E177_E0.5550.39
51_E60_A0.5540.39
110_V185_K0.5530.39
14_M57_L0.5520.39
125_G189_Y0.5520.39
6_E9_E0.5500.38
50_L108_I0.5480.38
25_A34_F0.5450.38
185_K194_E0.5440.38
40_S43_S0.5430.38
185_K190_A0.5430.38
101_L158_L0.5410.38
146_L183_T0.5380.37
110_V142_F0.5360.37
96_E155_D0.5350.37
9_E35_R0.5340.37
15_F25_A0.5310.37
80_A86_E0.5310.37
163_R172_G0.5290.36
79_T187_Y0.5280.36
18_V45_W0.5260.36
59_E62_A0.5230.36
12_W140_T0.5200.35
115_E170_K0.5190.35
45_W153_Y0.5190.35
123_L180_L0.5170.35
152_T158_L0.5170.35
76_M112_W0.5150.35
46_S160_L0.5140.35
189_Y195_A0.5140.35
129_P158_L0.5100.34
2_E6_E0.5100.34
81_P85_E0.5100.34
102_I158_L0.5080.34
135_D143_E0.5070.34
190_A193_E0.5070.34
64_G172_G0.5070.34
112_W130_L0.5070.34
112_W161_Y0.5050.34
132_S147_R0.5040.34
167_E190_A0.5020.34
41_Q128_R0.5010.34
158_L183_T0.5000.34
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2bb6A 1 0.5076 17.1 0.961 Contact Map
4ivnA 2 0.3401 11.6 0.964 Contact Map
2zc2A 1 0.3807 10.8 0.964 Contact Map
1lddA 4 0.2284 8.2 0.966 Contact Map
4ozvA 1 0.6802 8.2 0.966 Contact Map
1wpbA 6 0.4772 7.4 0.967 Contact Map
3iwfA 2 0.3503 7.2 0.967 Contact Map
2j96A 2 0.6599 5.8 0.968 Contact Map
2rnnA 1 0.2741 5.6 0.969 Contact Map
1kvdB 4 0.1472 5.3 0.969 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0074 seconds.