GREMLIN Database
DUF4032 - Domain of unknown function (DUF4032)
PFAM: PF13224 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 163 (162)
Sequences: 2541 (1410)
Seq/√Len: 110.8
META: 0.876

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
5_E26_R4.3761.00
4_D8_R3.7121.00
16_E19_R3.5111.00
157_S161_T2.9941.00
150_G153_E2.8261.00
88_R101_A2.7631.00
28_N82_N2.6891.00
109_V131_F2.6861.00
104_R108_E2.5461.00
137_H157_S2.4261.00
63_R85_D2.3171.00
65_L71_D2.2471.00
155_V159_V2.2181.00
129_E133_E2.2141.00
72_V128_A2.1731.00
97_D100_V2.1171.00
134_V155_V2.1001.00
21_E25_R2.0821.00
110_F128_A2.0291.00
113_V134_V2.0131.00
75_N78_R1.9441.00
13_G16_E1.9121.00
156_R160_A1.8191.00
141_L157_S1.8121.00
116_A156_R1.7901.00
87_Y108_E1.7601.00
10_E19_R1.7221.00
38_E49_R1.6671.00
92_E95_P1.6481.00
24_I34_V1.6321.00
5_E8_R1.6221.00
142_S150_G1.5921.00
79_R132_H1.5921.00
139_W143_E1.5721.00
63_R67_L1.5681.00
59_H62_R1.5481.00
117_V130_L1.5091.00
145_A153_E1.4591.00
85_D98_E1.4411.00
96_V101_A1.4391.00
136_E143_E1.4351.00
136_E139_W1.4331.00
63_R98_E1.4271.00
93_G96_V1.3911.00
5_E23_R1.3671.00
85_D88_R1.3641.00
94_R100_V1.3641.00
152_E155_V1.3611.00
117_V159_V1.3341.00
96_V100_V1.3301.00
140_Y143_E1.3281.00
75_N129_E1.3090.99
52_P55_V1.3050.99
42_D45_G1.2960.99
152_E156_R1.2750.99
93_G104_R1.2450.99
88_R98_E1.2120.99
42_D47_R1.1960.99
141_L149_V1.1660.99
60_H64_L1.1510.99
125_L133_E1.1410.99
134_V158_Y1.1310.98
12_F19_R1.1220.98
10_E23_R1.1200.98
111_E115_A1.1170.98
141_L145_A1.1090.98
19_R22_E1.0980.98
56_D65_L1.0810.98
157_S160_A1.0660.98
143_E150_G1.0570.98
17_R21_E1.0520.98
39_L48_L1.0370.97
57_A73_E1.0340.97
144_R161_T1.0280.97
91_L95_P1.0260.97
12_F16_E1.0220.97
142_S149_V1.0180.97
11_V49_R1.0030.97
149_V154_A0.9970.97
145_A149_V0.9950.97
9_E51_Q0.9620.96
87_Y104_R0.9420.95
61_R65_L0.9400.95
116_A155_V0.9400.95
150_G154_A0.9320.95
107_T111_E0.9260.95
91_L96_V0.9240.95
129_E136_E0.9210.95
106_L111_E0.9070.94
142_S145_A0.9010.94
110_F114_V0.8940.94
16_E22_E0.8930.94
156_R159_V0.8910.94
28_N59_H0.8850.93
58_G62_R0.8850.93
95_P101_A0.8830.93
103_H107_T0.8710.93
41_T45_G0.8690.93
70_L110_F0.8590.92
135_L138_R0.8540.92
136_E158_Y0.8430.92
20_I34_V0.8410.92
5_E10_E0.8410.92
97_D103_H0.8400.92
79_R140_Y0.8290.91
9_E49_R0.8270.91
12_F21_E0.8220.91
134_V151_L0.8200.91
138_R151_L0.8170.90
11_V47_R0.8140.90
53_K71_D0.8050.90
18_W25_R0.8050.90
111_E127_P0.8020.90
99_E107_T0.7970.89
40_V49_R0.7940.89
39_L42_D0.7900.89
136_E140_Y0.7860.89
33_D56_D0.7810.88
35_D53_K0.7780.88
61_R71_D0.7770.88
56_D71_D0.7700.88
79_R135_L0.7600.87
16_E21_E0.7550.87
80_L99_E0.7550.87
114_V130_L0.7540.86
52_P59_H0.7520.86
80_L133_E0.7470.86
52_P126_E0.7460.86
11_V17_R0.7380.85
70_L73_E0.7360.85
22_E25_R0.7360.85
148_D153_E0.7340.85
83_D138_R0.7300.85
18_W22_E0.7290.85
146_G150_G0.7280.84
17_R22_E0.7270.84
5_E30_L0.7250.84
84_L107_T0.7250.84
147_R153_E0.7230.84
63_R66_R0.7160.83
91_L101_A0.6940.82
2_L7_T0.6940.82
145_A148_D0.6930.81
74_E78_R0.6860.81
41_T46_T0.6850.81
58_G137_H0.6830.81
58_G118_P0.6830.81
128_A132_H0.6820.80
103_H111_E0.6800.80
24_I79_R0.6760.80
67_L102_A0.6750.80
17_R148_D0.6750.80
36_E80_L0.6730.80
118_P124_K0.6610.78
21_E48_L0.6570.78
130_L158_Y0.6550.78
70_L106_L0.6540.78
151_L158_Y0.6530.77
12_F18_W0.6520.77
33_D98_E0.6510.77
96_V104_R0.6510.77
14_P19_R0.6490.77
114_V159_V0.6470.77
58_G132_H0.6460.77
100_V103_H0.6450.77
37_L101_A0.6450.77
86_S138_R0.6440.77
46_T57_A0.6430.76
122_R127_P0.6430.76
152_E159_V0.6370.76
64_L72_V0.6320.75
88_R91_L0.6320.75
51_Q57_A0.6320.75
8_R42_D0.6290.75
39_L50_L0.6240.74
121_L124_K0.6200.74
64_L77_A0.6200.74
137_H141_L0.6180.74
53_K65_L0.6170.73
126_E129_E0.6170.73
23_R26_R0.6160.73
75_N84_L0.6150.73
151_L155_V0.6110.73
91_L94_R0.6050.72
34_V50_L0.6050.72
148_D154_A0.6000.71
92_E96_V0.5980.71
141_L151_L0.5980.71
133_E155_V0.5950.71
53_K66_R0.5900.70
24_I56_D0.5880.70
144_R157_S0.5880.70
10_E21_E0.5870.70
118_P125_L0.5860.70
13_G22_E0.5850.69
8_R14_P0.5850.69
55_V138_R0.5840.69
91_L104_R0.5840.69
93_G101_A0.5830.69
107_T152_E0.5830.69
20_I24_I0.5810.69
136_E157_S0.5760.68
63_R81_L0.5740.68
55_V59_H0.5720.68
61_R70_L0.5690.67
64_L80_L0.5640.67
14_P17_R0.5610.66
72_V79_R0.5600.66
110_F117_V0.5600.66
39_L46_T0.5600.66
98_E103_H0.5550.65
43_G46_T0.5540.65
118_P132_H0.5530.65
132_H140_Y0.5510.65
137_H140_Y0.5480.64
59_H133_E0.5450.64
36_E69_G0.5450.64
32_F78_R0.5430.64
94_R101_A0.5430.64
128_A133_E0.5430.64
38_E87_Y0.5420.64
9_E73_E0.5400.63
37_L61_R0.5400.63
74_E155_V0.5380.63
60_H77_A0.5380.63
144_R147_R0.5340.62
106_L126_E0.5340.62
28_N56_D0.5340.62
90_W110_F0.5310.62
6_L12_F0.5280.62
40_V47_R0.5270.61
66_R103_H0.5270.61
139_W142_S0.5250.61
33_D59_H0.5250.61
113_V155_V0.5250.61
21_E86_S0.5210.61
10_E13_G0.5180.60
113_V152_E0.5150.60
87_Y91_L0.5150.60
100_V104_R0.5140.60
118_P123_G0.5140.60
124_K127_P0.5130.59
63_R74_E0.5000.58
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3gfzA 2 0.6564 26.4 0.948 Contact Map
3mc9A 2 0 21.4 0.95 Contact Map
3q6bA 1 0.3436 13.9 0.954 Contact Map
1x9zA 4 0.7362 10.1 0.957 Contact Map
2ltaA 1 0.5399 8.2 0.959 Contact Map
2x8xX 1 0.362 7.9 0.959 Contact Map
2wxyC 2 0.8896 6.8 0.96 Contact Map
2fi0A 1 0.2331 6.3 0.961 Contact Map
3dp7A 2 0.3252 5.6 0.962 Contact Map
4q6jA 4 0.6319 5.4 0.962 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0067 seconds.