GREMLIN Database
DUF3971 - Protein of unknown function
PFAM: PF13116 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 288 (274)
Sequences: 8517 (6832)
Seq/√Len: 412.7
META: 0.824

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
123_D164_K3.0331.00
33_S62_E2.9201.00
60_D81_D2.8991.00
61_V64_A2.8281.00
182_T203_R2.7621.00
77_D97_R2.6601.00
121_E166_D2.4861.00
84_F89_L2.4811.00
34_D60_D2.4231.00
63_D77_D2.3831.00
180_S203_R2.3541.00
67_D74_P2.3531.00
125_E162_D2.2941.00
65_T74_P2.1601.00
180_S205_R2.0871.00
65_T76_E2.0711.00
90_E109_E2.0541.00
130_L155_A2.0541.00
183_L186_N2.0471.00
62_E79_D2.0421.00
31_R63_D2.0321.00
61_V80_G2.0081.00
199_D219_R1.9981.00
42_D45_D1.9861.00
59_A82_L1.9721.00
185_G199_D1.9691.00
127_S160_D1.9511.00
103_L106_V1.9411.00
156_S187_D1.9411.00
213_A216_L1.9211.00
17_P21_D1.8731.00
105_D125_E1.8551.00
79_D95_S1.8461.00
56_Q83_R1.8361.00
75_L78_L1.8211.00
3_A27_L1.8151.00
119_V166_D1.8131.00
58_T81_D1.8061.00
28_V65_T1.7831.00
135_R139_A1.7801.00
22_W66_L1.7711.00
93_A106_V1.7031.00
21_D25_R1.6621.00
218_A225_V1.6601.00
133_A159_A1.6601.00
31_R62_E1.6531.00
58_T83_R1.6491.00
97_R102_R1.6411.00
56_Q85_D1.6151.00
171_D174_D1.6111.00
67_D71_G1.5991.00
100_G132_D1.5971.00
32_V35_G1.5881.00
161_L181_V1.5881.00
101_V126_A1.5861.00
87_N112_D1.5501.00
247_T270_P1.5481.00
88_G113_L1.5481.00
179_G206_F1.5371.00
132_D135_R1.5351.00
164_K178_D1.5161.00
92_D107_T1.4991.00
6_V10_L1.4961.00
120_L169_L1.4931.00
159_A181_V1.4901.00
104_S127_S1.4691.00
249_D252_A1.4681.00
187_D198_E1.4661.00
35_G59_A1.4651.00
207_T210_G1.4521.00
20_R24_D1.4441.00
184_A201_N1.4401.00
202_G216_L1.4261.00
109_E121_E1.4211.00
219_R224_P1.4191.00
80_G91_I1.4081.00
220_L225_V1.4041.00
106_V124_G1.4021.00
217_T226_T1.3871.00
57_V84_F1.3841.00
99_G132_D1.3821.00
243_D274_T1.3711.00
88_G109_E1.3641.00
127_S158_N1.3631.00
214_K228_D1.3631.00
176_K208_E1.3621.00
90_E107_T1.3611.00
23_L27_L1.3471.00
130_L159_A1.3421.00
16_G19_L1.3381.00
7_K24_D1.3251.00
213_A229_L1.3231.00
130_L186_N1.3181.00
99_G135_R1.3131.00
28_V67_D1.3031.00
129_P158_N1.2961.00
121_E164_K1.2881.00
108_A122_I1.2841.00
205_R212_S1.2741.00
183_L204_L1.2671.00
68_Y98_I1.2641.00
36_R58_T1.2461.00
241_R276_T1.2451.00
123_D162_D1.2441.00
125_E160_D1.2431.00
247_T268_S1.2431.00
38_R54_E1.2391.00
244_A273_A1.2381.00
134_L138_R1.2381.00
5_A8_R1.2381.00
49_D87_N1.2371.00
251_A254_R1.2371.00
19_L23_L1.2311.00
154_Q189_R1.2271.00
68_Y136_F1.2271.00
91_I108_A1.2211.00
97_R100_G1.2191.00
28_V74_P1.2171.00
107_T123_D1.1961.00
245_Q272_Q1.1911.00
101_V132_D1.1831.00
25_R67_D1.1791.00
134_L155_A1.1751.00
241_R274_T1.1741.00
129_P132_D1.1611.00
189_R196_P1.1581.00
79_D94_A1.1561.00
160_D182_T1.1511.00
38_R56_Q1.1481.00
126_A161_L1.1431.00
98_I136_F1.1411.00
166_D176_K1.1411.00
185_G201_N1.1381.00
162_D180_S1.1361.00
197_L218_A1.1221.00
89_L113_L1.1151.00
223_G268_S1.1151.00
111_P121_E1.1101.00
64_A78_L1.1101.00
246_G271_W1.1071.00
256_A260_P1.1021.00
76_E97_R1.1011.00
254_R262_A1.0771.00
270_P273_A1.0741.00
82_L89_L1.0731.00
215_G228_D1.0471.00
10_L15_L1.0451.00
178_D205_R1.0451.00
48_F53_G1.0441.00
83_R92_D1.0431.00
29_A65_T1.0381.00
143_G151_D1.0281.00
61_V82_L1.0221.00
81_D92_D1.0191.00
167_L175_T1.0171.00
46_F53_G1.0071.00
199_D202_G0.9971.00
95_S102_R0.9811.00
33_S60_D0.9811.00
15_L20_R0.9801.00
47_P53_G0.9781.00
148_G151_D0.9711.00
78_L96_G0.9571.00
179_G183_L0.9401.00
101_V128_G0.9381.00
248_A269_A0.9361.00
251_A255_A0.9251.00
66_L78_L0.9221.00
138_R151_D0.9191.00
124_G163_L0.9121.00
80_G94_A0.9121.00
99_G139_A0.9101.00
13_L20_R0.9091.00
187_D196_P0.8941.00
32_V59_A0.8921.00
205_R214_K0.8891.00
137_L140_S0.8871.00
179_G185_G0.8861.00
81_D94_A0.8851.00
228_D243_D0.8841.00
83_R90_E0.8811.00
249_D268_S0.8801.00
198_E219_R0.8761.00
181_V186_N0.8761.00
67_D70_P0.8751.00
54_E85_D0.8701.00
115_A118_P0.8681.00
88_G111_P0.8661.00
160_D184_A0.8601.00
186_N190_L0.8581.00
227_L244_A0.8551.00
40_R54_E0.8551.00
117_D170_K0.8531.00
75_L96_G0.8531.00
105_D108_A0.8521.00
215_G226_T0.8411.00
112_D115_A0.8401.00
172_G175_T0.8311.00
108_A120_L0.8281.00
202_G206_F0.8281.00
183_L202_G0.8201.00
197_L200_L0.8171.00
240_V244_A0.8161.00
167_L172_G0.8151.00
89_L110_I0.8141.00
218_A227_L0.8101.00
200_L218_A0.8041.00
102_R127_S0.8021.00
26_A67_D0.8011.00
188_L200_L0.7991.00
78_L93_A0.7981.00
138_R143_G0.7931.00
216_L227_L0.7901.00
39_L43_L0.7891.00
259_L263_D0.7881.00
84_F113_L0.7881.00
39_L42_D0.7871.00
120_L167_L0.7861.00
18_G21_D0.7821.00
250_A254_R0.7811.00
256_A259_L0.7741.00
63_D79_D0.7731.00
46_F55_F0.7671.00
258_G262_A0.7661.00
131_A155_A0.7621.00
142_L145_L0.7611.00
72_W141_P0.7591.00
103_L124_G0.7571.00
41_G48_F0.7551.00
224_P227_L0.7541.00
234_K238_G0.7511.00
54_E86_G0.7491.00
135_R138_R0.7461.00
84_F88_G0.7451.00
39_L48_F0.7411.00
241_R246_G0.7401.00
235_P238_G0.7371.00
191_P194_G0.7361.00
185_G192_P0.7321.00
37_L57_V0.7311.00
92_D105_D0.7261.00
131_A135_R0.7261.00
143_G146_L0.7261.00
226_T245_Q0.7241.00
77_D95_S0.7231.00
93_A96_G0.7181.00
117_D171_D0.7181.00
142_L146_L0.7151.00
65_T77_D0.7121.00
133_A161_L0.7111.00
60_D79_D0.7101.00
75_L103_L0.7081.00
233_G236_G0.7081.00
243_D272_Q0.7081.00
178_D207_T0.7061.00
130_L133_A0.7051.00
128_G132_D0.7051.00
75_L124_G0.7051.00
96_G103_L0.7051.00
26_A70_P0.7031.00
177_V208_E0.7031.00
194_G199_D0.7011.00
233_G237_D0.7001.00
144_E148_G0.7001.00
217_T224_P0.6981.00
153_L190_L0.6921.00
229_L242_I0.6911.00
10_L20_R0.6901.00
4_A29_A0.6851.00
185_G188_L0.6841.00
158_N185_G0.6831.00
219_R222_G0.6821.00
27_L64_A0.6811.00
202_G213_A0.6781.00
202_G227_L0.6741.00
103_L126_A0.6741.00
252_A255_A0.6741.00
254_R265_L0.6690.99
114_G117_D0.6680.99
57_V82_L0.6660.99
190_L194_G0.6660.99
143_G148_G0.6660.99
110_I120_L0.6660.99
74_P98_I0.6610.99
47_P55_F0.6540.99
75_L108_A0.6530.99
200_L216_L0.6530.99
195_P206_F0.6430.99
232_R237_D0.6420.99
229_L271_W0.6410.99
266_S271_W0.6390.99
242_I277_I0.6370.99
165_L177_V0.6370.99
259_L262_A0.6360.99
45_D48_F0.6320.99
232_R238_G0.6320.99
140_S143_G0.6310.99
145_L148_G0.6290.99
66_L75_L0.6270.99
80_G93_A0.6260.99
26_A66_L0.6250.99
271_W275_L0.6250.99
133_A137_L0.6220.99
126_A133_A0.6220.99
261_L264_R0.6170.99
162_D182_T0.6150.99
104_S125_E0.6090.99
177_V190_L0.6090.99
190_L193_L0.6020.99
72_W140_S0.6020.99
204_L229_L0.6010.99
204_L213_A0.6010.99
193_L196_P0.6000.99
97_R132_D0.5990.99
87_N114_G0.5980.99
146_L149_A0.5950.99
233_G238_G0.5920.99
155_A188_L0.5910.99
110_I113_L0.5900.99
25_R70_P0.5860.99
223_G246_G0.5850.99
27_L82_L0.5850.99
34_D58_T0.5840.99
30_G64_A0.5820.99
187_D199_D0.5820.99
234_K237_D0.5800.99
220_L273_A0.5790.98
64_A68_Y0.5770.98
199_D217_T0.5770.98
25_R28_V0.5770.98
66_L91_I0.5740.98
213_A228_D0.5720.98
177_V181_V0.5700.98
75_L98_I0.5690.98
130_L181_V0.5680.98
14_L20_R0.5670.98
28_V31_R0.5660.98
154_Q191_P0.5660.98
6_V9_Y0.5650.98
53_G86_G0.5640.98
245_Q270_P0.5640.98
260_P263_D0.5640.98
240_V248_A0.5630.98
200_L204_L0.5630.98
183_L188_L0.5620.98
242_I246_G0.5610.98
68_Y75_L0.5600.98
101_V133_A0.5600.98
61_V78_L0.5590.98
114_G118_P0.5590.98
255_A260_P0.5550.98
19_L27_L0.5550.98
19_L89_L0.5550.98
69_A167_L0.5540.98
186_N189_R0.5530.98
104_S108_A0.5520.98
120_L124_G0.5510.98
223_G271_W0.5480.98
187_D190_L0.5470.98
69_A72_W0.5470.98
75_L122_I0.5450.98
242_I248_A0.5450.98
201_N217_T0.5440.98
122_I165_L0.5420.98
39_L46_F0.5410.98
68_Y72_W0.5390.98
21_D24_D0.5380.98
128_G133_A0.5380.98
45_D52_E0.5360.98
236_G239_A0.5360.98
109_E123_D0.5340.97
216_L231_T0.5330.97
269_A273_A0.5320.97
179_G186_N0.5300.97
255_A262_A0.5280.97
254_R263_D0.5280.97
185_G190_L0.5270.97
262_A265_L0.5250.97
195_P202_G0.5240.97
96_G106_V0.5240.97
252_A256_A0.5240.97
129_P156_S0.5230.97
116_K171_D0.5220.97
142_L165_L0.5220.97
29_A63_D0.5220.97
93_A108_A0.5210.97
4_A28_V0.5200.97
98_I101_V0.5190.97
263_D268_S0.5160.97
156_S189_R0.5150.97
73_P99_G0.5150.97
220_L224_P0.5140.97
110_I169_L0.5120.97
230_G243_D0.5120.97
108_A113_L0.5110.97
134_L137_L0.5110.97
15_L19_L0.5100.97
39_L86_G0.5100.97
178_D208_E0.5100.97
267_G271_W0.5090.97
138_R148_G0.5070.97
243_D276_T0.5060.97
238_G241_R0.5060.97
64_A80_G0.5060.97
175_T179_G0.5040.96
6_V20_R0.5040.96
256_A262_A0.5040.96
212_S215_G0.5020.96
17_P20_R0.5010.96
177_V183_L0.5010.96
147_G153_L0.5000.96
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4yguA 3 0.5938 5.7 0.95 Contact Map
2cfuA 2 0.4514 3.9 0.954 Contact Map
1d8jA 1 0.1597 3.3 0.956 Contact Map
1k3kA 1 0.1389 2.7 0.958 Contact Map
1poiA 3 0.2188 2.6 0.958 Contact Map
2ks0A 2 0.2292 2.5 0.958 Contact Map
4nurA 2 0.4444 2.3 0.959 Contact Map
1go9A 1 0.1354 2.1 0.96 Contact Map
4kyzA 1 0.2118 2.1 0.96 Contact Map
1qysA 1 0.2118 2 0.961 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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