GREMLIN Database
DUF3866 - Protein of unknown function (DUF3866)
PFAM: PF12982 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 319 (316)
Sequences: 1964 (1356)
Seq/√Len: 76.3
META: 0.827

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
89_M178_A5.4841.00
149_A165_A4.6351.00
145_G173_L4.4421.00
232_V237_L4.0101.00
107_L177_V3.6611.00
138_R156_D3.5921.00
69_V159_A3.2321.00
208_A246_A3.1791.00
128_L134_V3.1681.00
104_A179_V2.8471.00
160_V189_T2.7991.00
201_G243_V2.7191.00
128_L146_T2.6781.00
25_V35_H2.6521.00
205_N245_L2.6061.00
108_K144_A2.5991.00
104_A116_V2.5901.00
19_L39_A2.4671.00
94_A181_A2.3611.00
107_L116_V2.3451.00
204_V248_A2.3071.00
167_L193_W2.2631.00
72_Q77_H2.2611.00
38_V61_P2.2371.00
69_V168_A2.1851.00
213_R249_D2.1731.00
111_R114_A2.1061.00
116_V177_V2.0491.00
304_G307_E2.0481.00
247_P277_R2.0471.00
218_L236_T2.0361.00
221_S308_D2.0331.00
220_I310_A2.0251.00
202_E205_N2.0031.00
54_I187_V1.9881.00
107_L114_A1.9741.00
117_A175_A1.9721.00
204_V244_A1.9401.00
136_A140_R1.9191.00
89_M170_R1.8951.00
182_Q186_I1.8941.00
54_I68_A1.8871.00
117_A173_L1.8851.00
315_A319_G1.8811.00
92_V103_V1.8511.00
83_A167_L1.8321.00
192_R242_R1.8021.00
93_V203_A1.7661.00
100_L181_A1.7231.00
57_L60_T1.7201.00
117_A145_G1.7041.00
180_V203_A1.6891.00
148_T155_G1.6491.00
69_V172_V1.6451.00
4_A9_T1.6321.00
204_V216_A1.6191.00
108_K142_L1.6161.00
186_I195_F1.6071.00
27_L65_A1.6021.00
94_A217_A1.5870.99
201_G205_N1.5840.99
145_G157_L1.5710.99
216_A250_V1.5580.99
93_V207_A1.5560.99
186_I197_G1.5520.99
123_G182_Q1.5290.99
103_V179_V1.5100.99
95_D99_Q1.5020.99
16_D40_N1.4880.99
119_V165_A1.4870.99
122_D151_Q1.4860.99
7_A129_A1.4850.99
103_V217_A1.4850.99
34_Y130_F1.4790.99
17_R42_D1.4750.99
249_D277_R1.4600.99
259_G263_R1.4600.99
80_L83_A1.4560.99
67_L73_E1.4390.99
116_V179_V1.4320.99
108_K116_V1.4310.99
93_V200_A1.4300.99
74_S77_H1.4270.99
222_F310_A1.4240.99
222_F309_P1.4230.99
94_A103_V1.4180.99
79_V171_H1.4140.99
106_G319_G1.3870.99
22_T26_D1.3850.99
67_L74_S1.3710.98
287_L316_A1.3640.98
151_Q186_I1.3530.98
255_L262_V1.3510.98
101_P137_L1.3340.98
151_Q182_Q1.3330.98
261_R265_Q1.3280.98
292_A306_D1.3190.98
182_Q199_A1.3150.98
200_A216_A1.3140.98
38_V62_L1.3130.98
220_I314_A1.3110.98
84_D192_R1.2930.98
218_L232_V1.2910.98
209_A245_L1.2910.98
104_A142_L1.2860.98
43_R46_A1.2830.98
149_A152_A1.2700.97
108_K113_G1.2420.97
272_L276_H1.2340.97
157_L168_A1.2310.97
160_V187_V1.2250.97
151_Q187_V1.2130.97
170_R178_A1.2070.97
205_N244_A1.2010.96
93_V204_V1.1970.96
244_A248_A1.1870.96
288_L305_L1.1870.96
134_V143_L1.1820.96
208_A214_P1.1700.96
163_H206_A1.1560.96
254_A280_E1.1560.96
227_E230_R1.1490.95
255_L310_A1.1470.95
134_V146_T1.1450.95
4_A12_V1.1440.95
148_T158_E1.1360.95
262_V265_Q1.1340.95
77_H81_R1.1280.95
120_M148_T1.1250.95
193_W243_V1.1160.95
40_N43_R1.1070.94
283_D286_G1.0960.94
202_E245_L1.0850.94
251_P281_V1.0780.94
104_A143_L1.0770.94
264_A268_A1.0750.93
3_L34_Y1.0740.93
80_L167_L1.0680.93
217_A240_L1.0660.93
87_D170_R1.0550.93
200_A239_A1.0510.93
101_P133_T1.0440.92
46_A49_P1.0440.92
205_N246_A1.0430.92
300_T305_L1.0330.92
117_A178_A1.0320.92
83_A171_H1.0220.92
6_P10_G1.0150.91
99_Q315_A1.0130.91
119_V147_V1.0120.91
284_G287_L1.0060.91
207_A212_G1.0010.91
122_D150_G1.0000.91
216_A236_T0.9990.91
264_A267_A0.9900.90
199_A203_A0.9870.90
263_R267_A0.9840.90
217_A315_A0.9800.90
23_T65_A0.9800.90
123_G151_Q0.9780.90
69_V80_L0.9760.90
95_D200_A0.9730.90
154_G158_E0.9710.89
236_T240_L0.9680.89
161_T198_V0.9630.89
253_P314_A0.9610.89
148_T154_G0.9530.89
167_L171_H0.9520.89
288_L309_P0.9510.88
99_Q217_A0.9420.88
216_A239_A0.9340.88
120_M152_A0.9270.87
250_V276_H0.9240.87
67_L75_P0.9240.87
4_A10_G0.9200.87
102_P133_T0.9180.87
56_K158_E0.9150.86
162_V203_A0.9120.86
67_L72_Q0.9100.86
99_Q311_F0.9100.86
272_L278_L0.9070.86
281_V284_G0.9060.86
94_A100_L0.9040.86
193_W242_R0.8880.85
138_R145_G0.8830.84
166_L178_A0.8810.84
53_H67_L0.8810.84
193_W209_A0.8750.84
132_R135_A0.8720.84
182_Q196_S0.8720.84
107_L319_G0.8650.83
56_K150_G0.8570.83
300_T308_D0.8540.82
198_V243_V0.8530.82
255_L259_G0.8490.82
288_L292_A0.8470.82
102_P311_F0.8400.81
72_Q190_G0.8390.81
129_A153_F0.8370.81
41_L45_P0.8350.81
77_H191_T0.8350.81
208_A245_L0.8300.81
149_A159_A0.8270.80
84_D242_R0.8190.80
205_N243_V0.8180.80
263_R280_E0.8170.80
110_A319_G0.8160.79
52_G71_E0.8150.79
202_E209_A0.8100.79
201_G244_A0.8060.79
94_A179_V0.8030.78
29_L32_G0.8020.78
204_V215_V0.8000.78
138_R146_T0.8000.78
261_R264_A0.7990.78
106_G110_A0.7970.78
16_D43_R0.7950.78
78_E81_R0.7940.78
123_G196_S0.7930.77
100_L137_L0.7920.77
205_N209_A0.7920.77
142_L294_S0.7920.77
86_L210_L0.7910.77
187_V196_S0.7890.77
300_T309_P0.7860.77
99_Q193_W0.7860.77
21_N27_L0.7850.77
273_A278_L0.7830.77
100_L134_V0.7820.77
57_L161_T0.7810.76
234_H238_T0.7810.76
69_V157_L0.7790.76
78_E82_D0.7770.76
119_V180_V0.7740.76
106_G287_L0.7720.76
128_L148_T0.7710.75
145_G156_D0.7690.75
165_A168_A0.7680.75
182_Q195_F0.7600.74
151_Q196_S0.7600.74
171_H178_A0.7580.74
44_L49_P0.7540.74
256_P263_R0.7540.74
9_T12_V0.7530.74
147_V173_L0.7500.73
133_T298_L0.7440.73
147_V157_L0.7430.73
147_V169_A0.7420.73
272_L280_E0.7420.73
6_P9_T0.7320.72
225_P230_R0.7300.71
136_A139_E0.7290.71
205_N208_A0.7240.71
92_V217_A0.7230.71
135_A139_E0.7230.71
102_P298_L0.7190.70
287_L290_A0.7180.70
43_R47_D0.7170.70
260_A264_A0.7150.70
255_L317_A0.7130.70
21_N24_A0.7110.69
129_A148_T0.7100.69
265_Q268_A0.7090.69
86_L212_G0.7090.69
31_T133_T0.7080.69
119_V149_A0.7070.69
5_Y9_T0.7050.69
86_L89_M0.7050.69
182_Q203_A0.7050.69
20_L35_H0.7020.68
3_L225_P0.7000.68
263_R266_L0.7000.68
121_T149_A0.7000.68
272_L275_R0.6990.68
158_E169_A0.6990.68
288_L306_D0.6990.68
95_D196_S0.6970.68
280_E283_D0.6960.68
105_A164_S0.6950.68
312_F319_G0.6910.67
216_A248_A0.6900.67
29_L33_G0.6890.67
127_P153_F0.6870.67
40_N44_L0.6860.67
220_I250_V0.6850.67
144_A173_L0.6840.66
105_A142_L0.6830.66
244_A276_H0.6820.66
231_G265_Q0.6780.66
259_G266_L0.6770.66
31_T57_L0.6760.66
313_L316_A0.6740.65
281_V287_L0.6720.65
159_A164_S0.6700.65
290_A294_S0.6690.65
239_A244_A0.6670.65
300_T307_E0.6660.64
59_Y63_Q0.6660.64
76_H79_V0.6640.64
103_V204_V0.6610.64
90_P212_G0.6600.64
170_R212_G0.6560.63
250_V272_L0.6540.63
9_T38_V0.6540.63
57_L195_F0.6530.63
277_R280_E0.6530.63
72_Q75_P0.6530.63
9_T39_A0.6510.63
204_V208_A0.6510.63
147_V168_A0.6450.62
227_E261_R0.6430.62
22_T35_H0.6420.62
21_N36_F0.6390.61
196_S199_A0.6390.61
149_A162_V0.6380.61
289_E292_A0.6380.61
163_H198_V0.6380.61
99_Q218_L0.6360.61
217_A252_V0.6360.61
129_A154_G0.6360.61
162_V180_V0.6350.61
26_D47_D0.6350.61
43_R49_P0.6340.61
77_H80_L0.6340.61
92_V133_T0.6330.60
214_P246_A0.6320.60
199_A202_E0.6310.60
288_L313_L0.6280.60
30_G33_G0.6260.60
95_D197_G0.6260.60
252_V266_L0.6220.59
22_T36_F0.6220.59
44_L48_P0.6220.59
44_L47_D0.6220.59
137_L143_L0.6210.59
147_V307_E0.6210.59
22_T25_V0.6200.59
19_L44_L0.6200.59
232_V262_V0.6190.59
163_H202_E0.6180.59
35_H182_Q0.6140.58
299_S308_D0.6140.58
200_A240_L0.6120.58
80_L171_H0.6110.58
237_L241_G0.6100.58
291_L295_G0.6090.58
41_L44_L0.6050.57
251_P284_G0.6040.57
43_R50_G0.6020.57
92_V251_P0.6010.57
105_A294_S0.6010.57
294_S297_P0.6010.57
105_A296_L0.6010.57
36_F187_V0.5990.56
286_G290_A0.5980.56
95_D186_I0.5970.56
270_A273_A0.5960.56
60_T153_F0.5940.56
54_I196_S0.5930.56
105_A140_R0.5930.56
258_E309_P0.5920.55
212_G215_V0.5920.55
107_L179_V0.5910.55
257_G260_A0.5880.55
51_G67_L0.5860.55
257_G285_G0.5850.55
56_K59_Y0.5850.55
57_L311_F0.5850.55
305_L313_L0.5820.54
157_L173_L0.5810.54
310_A313_L0.5790.54
106_G316_A0.5760.53
5_Y39_A0.5760.53
239_A242_R0.5750.53
121_T203_A0.5740.53
58_R151_Q0.5740.53
117_A144_A0.5710.53
80_L86_L0.5700.53
86_L150_G0.5690.53
117_A169_A0.5680.52
205_N239_A0.5680.52
222_F255_L0.5680.52
123_G186_I0.5640.52
86_L171_H0.5640.52
105_A298_L0.5630.52
57_L64_H0.5630.52
46_A50_G0.5620.52
19_L60_T0.5620.52
6_P11_P0.5620.52
115_R176_D0.5610.52
209_A243_V0.5600.51
153_F158_E0.5590.51
223_A300_T0.5590.51
271_G314_A0.5590.51
52_G122_D0.5580.51
48_P51_G0.5560.51
65_A151_Q0.5550.51
4_A20_L0.5550.51
209_A241_G0.5540.51
140_R296_L0.5540.51
22_T255_L0.5530.51
291_L298_L0.5510.50
130_F157_L0.5500.50
204_V245_L0.5500.50
92_V315_A0.5490.50
223_A230_R0.5490.50
231_G237_L0.5480.50
77_H190_G0.5480.50
72_Q197_G0.5470.50
3_L217_A0.5460.50
52_G56_K0.5460.50
109_A290_A0.5440.49
256_P259_G0.5430.49
163_H209_A0.5430.49
139_E157_L0.5430.49
164_S167_L0.5420.49
121_T199_A0.5410.49
150_G158_E0.5410.49
249_D279_V0.5410.49
128_L207_A0.5400.49
115_R174_G0.5400.49
171_H192_R0.5400.49
114_A177_V0.5380.49
220_I311_F0.5360.48
220_I253_P0.5360.48
73_E77_H0.5340.48
104_A177_V0.5340.48
12_V40_N0.5340.48
303_R308_D0.5330.48
313_L317_A0.5320.48
92_V105_A0.5310.48
255_L313_L0.5290.47
163_H243_V0.5290.47
94_A99_Q0.5270.47
150_G154_G0.5270.47
93_V182_Q0.5270.47
162_V199_A0.5270.47
284_G313_L0.5250.47
204_V241_G0.5250.47
60_T112_P0.5240.47
5_Y61_P0.5230.47
69_V161_T0.5230.47
22_T27_L0.5230.47
238_T242_R0.5230.47
300_T311_F0.5210.46
168_A173_L0.5200.46
56_K154_G0.5200.46
102_P137_L0.5200.46
53_H71_E0.5200.46
259_G280_E0.5200.46
255_L258_E0.5190.46
175_A178_A0.5170.46
220_I236_T0.5170.46
95_D193_W0.5170.46
114_A117_A0.5150.46
100_L133_T0.5150.46
220_I262_V0.5150.46
2_A37_V0.5150.46
146_T156_D0.5140.46
103_V315_A0.5120.45
123_G210_L0.5110.45
309_P313_L0.5080.45
114_A176_D0.5070.45
13_E43_R0.5060.45
8_L276_H0.5060.45
54_I71_E0.5050.44
6_P12_V0.5050.44
81_R192_R0.5040.44
121_T157_L0.5040.44
23_T52_G0.5040.44
74_S191_T0.5030.44
95_D190_G0.5030.44
218_L252_V0.5010.44
35_H65_A0.5000.44
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2q28A 4 0.5893 31 0.977 Contact Map
3esiA 4 0.1755 26.3 0.977 Contact Map
2r751 1 0.6771 21.2 0.978 Contact Map
3r6mA 2 0.5423 18.1 0.979 Contact Map
4l4yA 2 0.6803 16.8 0.979 Contact Map
2im9A 1 0.2915 15.6 0.98 Contact Map
2vd8A 2 0.1944 14 0.98 Contact Map
4q9dA 4 0.6865 13.4 0.98 Contact Map
2p13A 2 0.1348 13.1 0.98 Contact Map
3hgjA 2 0.8433 11.3 0.981 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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