GREMLIN Database
DUF3849 - Protein of unknown function (DUF3849)
PFAM: PF12960 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 124 (121)
Sequences: 1239 (953)
Seq/√Len: 86.7
META: 0.958

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
70_V116_I3.7431.00
110_A114_G3.4681.00
73_K79_F3.3851.00
8_Y12_H3.0081.00
33_E105_V2.3541.00
24_A27_A2.2961.00
81_R85_E2.2391.00
83_N120_R2.1661.00
77_G84_K2.1171.00
35_A53_V2.0361.00
78_R108_H2.0041.00
63_N119_A1.9431.00
6_A15_L1.9191.00
36_I115_F1.9071.00
29_A33_E1.8741.00
73_K106_D1.8181.00
113_D117_N1.7971.00
83_N117_N1.7231.00
80_S83_N1.6551.00
4_Y8_Y1.6541.00
40_Y43_N1.6141.00
31_A56_E1.5821.00
79_F109_P1.5781.00
15_L19_R1.5541.00
10_R15_L1.5351.00
34_K38_E1.5311.00
40_Y45_L1.5191.00
31_A57_Y1.4981.00
58_G62_V1.4840.99
82_D85_E1.4750.99
13_G16_E1.4490.99
70_V87_A1.4340.99
74_D88_K1.4250.99
83_N113_D1.4180.99
22_R26_I1.4080.99
8_Y11_E1.4050.99
6_A10_R1.3770.99
29_A65_V1.3640.99
27_A30_E1.3370.99
71_Q92_I1.3270.99
31_A35_A1.3110.99
59_F63_N1.3080.99
53_V62_V1.3060.99
39_N46_D1.2830.98
54_I59_F1.2670.98
54_I58_G1.2660.98
76_D108_H1.2450.98
64_Y93_P1.2420.98
111_L114_G1.2130.98
49_A53_V1.2000.98
39_N52_E1.1970.98
40_Y111_L1.1910.97
32_I115_F1.1650.97
67_A90_F1.1420.97
10_R19_R1.1410.97
85_E88_K1.1220.96
6_A19_R1.1010.96
63_N90_F1.0800.96
2_Y14_E1.0740.95
7_E11_E1.0690.95
43_N111_L1.0540.95
70_V84_K1.0260.94
32_I53_V1.0220.94
51_K122_E1.0060.93
21_S25_N1.0050.93
68_N92_I1.0040.93
38_E52_E0.9940.93
51_K55_E0.9770.92
100_N103_F0.9740.92
80_S113_D0.9690.92
53_V115_F0.9640.92
1_V25_N0.9600.92
107_S112_L0.9570.92
118_Q121_K0.9520.91
64_Y103_F0.9450.91
52_E56_E0.9420.91
25_N61_R0.9330.91
50_A115_F0.9300.90
31_A53_V0.9270.90
34_K37_R0.9190.90
105_V112_L0.9070.89
26_I29_A0.8870.88
40_Y110_A0.8830.88
5_S8_Y0.8780.88
22_R102_D0.8570.86
68_N100_N0.8520.86
30_E33_E0.8390.85
76_D84_K0.8350.85
83_N86_W0.7770.81
40_Y44_R0.7770.81
73_K109_P0.7670.80
94_E100_N0.7650.80
48_D51_K0.7600.79
68_N103_F0.7590.79
110_A113_D0.7490.78
95_D100_N0.7350.77
21_S61_R0.7330.77
68_N72_Q0.7330.77
72_Q100_N0.7290.76
16_E20_A0.7170.75
69_T112_L0.7170.75
49_A52_E0.7040.74
26_I65_V0.6910.72
54_I62_V0.6900.72
3_P12_H0.6860.72
66_L119_A0.6860.72
101_R104_V0.6830.71
46_D49_A0.6780.71
4_Y12_H0.6780.71
66_L116_I0.6780.71
84_K87_A0.6740.70
25_N103_F0.6720.70
50_A119_A0.6690.70
7_E10_R0.6630.69
117_N120_R0.6600.69
64_Y92_I0.6590.69
19_R22_R0.6560.68
50_A62_V0.6540.68
36_I107_S0.6530.68
1_V8_Y0.6500.68
58_G66_L0.6350.66
113_D120_R0.6310.65
62_V86_W0.6280.65
3_P64_Y0.6270.65
108_H113_D0.6260.65
79_F110_A0.6250.65
18_Y26_I0.6240.65
5_S11_E0.6240.65
32_I62_V0.6180.64
49_A118_Q0.6170.64
76_D83_N0.6160.64
12_H60_E0.6130.63
69_T109_P0.6090.63
107_S114_G0.6050.62
41_D44_R0.6050.62
26_I102_D0.6010.62
82_D117_N0.5980.61
18_Y22_R0.5910.60
59_F119_A0.5910.60
5_S12_H0.5840.59
48_D54_I0.5830.59
5_S18_Y0.5750.58
30_E34_K0.5710.58
12_H17_L0.5700.58
79_F114_G0.5690.57
2_Y9_A0.5680.57
45_L118_Q0.5650.57
118_Q122_E0.5630.57
24_A57_Y0.5610.56
26_I57_Y0.5580.56
13_G17_L0.5560.56
94_E98_D0.5490.55
62_V119_A0.5480.55
78_R84_K0.5470.55
30_E85_E0.5400.54
18_Y79_F0.5400.54
28_C61_R0.5400.54
107_S111_L0.5400.54
85_E94_E0.5400.54
58_G119_A0.5380.53
58_G61_R0.5350.53
33_E107_S0.5330.53
14_E19_R0.5290.52
99_R103_F0.5280.52
36_I86_W0.5240.51
10_R75_W0.5230.51
69_T104_V0.5210.51
61_R65_V0.5200.51
56_E64_Y0.5190.51
35_A52_E0.5170.51
41_D48_D0.5160.50
70_V112_L0.5150.50
59_F62_V0.5140.50
29_A69_T0.5140.50
9_A21_S0.5140.50
56_E70_V0.5110.50
108_H114_G0.5110.50
6_A11_E0.5100.50
69_T79_F0.5080.49
64_Y68_N0.5030.49
94_E104_V0.5030.49
79_F112_L0.5000.48
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3tduA 1 0.7339 9 0.932 Contact Map
1wloA 1 0.6371 8 0.933 Contact Map
2es9A 1 0.6774 6.5 0.936 Contact Map
3kevA 1 0.7097 6.4 0.936 Contact Map
4po5A 1 0.4919 6.2 0.937 Contact Map
3t5vB 1 0.8871 5.9 0.937 Contact Map
4fkcA 2 0.9597 5.5 0.938 Contact Map
3lyhA 2 0.871 4.7 0.94 Contact Map
3v57A 1 0.5 4.6 0.94 Contact Map
2vmlA 3 0.5081 4.2 0.941 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0057 seconds.