GREMLIN Database
DUF3825 - Domain of unknown function (DUF3825)
PFAM: PF12873 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 239 (236)
Sequences: 830 (704)
Seq/√Len: 45.8
META: 0.865

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
221_T224_M7.7261.00
117_F123_L4.9081.00
30_T108_Y3.9601.00
33_R37_E3.7681.00
157_E161_E3.3921.00
34_L106_A3.3861.00
68_E81_K3.1711.00
36_E184_G2.9281.00
64_Y102_L2.9091.00
167_V190_L2.8711.00
177_Q228_N2.8361.00
105_R169_R2.7751.00
138_F202_L2.7001.00
37_E107_N2.6621.00
129_H218_T2.5981.00
70_N76_Q2.4721.00
174_A190_L2.3671.00
140_E143_Q2.3631.00
194_L201_D2.2561.00
65_A83_F2.1911.00
56_V169_R2.1841.00
70_N74_G2.1361.00
95_L99_F2.0931.00
177_Q187_Q1.9560.99
24_K28_E1.9260.99
70_N73_P1.8830.99
220_L228_N1.8640.99
125_V160_I1.8340.99
70_N75_R1.7420.98
31_F49_A1.7420.98
104_E168_R1.6690.98
123_L206_V1.6280.97
175_V233_A1.6190.97
138_F156_L1.6190.97
11_D19_P1.5960.97
132_E149_D1.5960.97
34_L37_E1.5780.97
177_Q189_L1.5690.97
6_L60_Y1.5340.96
174_A188_L1.5000.96
71_R79_F1.4870.95
185_K207_E1.4750.95
12_Y21_P1.4740.95
121_L208_K1.4660.95
221_T227_N1.4630.95
187_Q228_N1.4630.95
172_K236_D1.4590.95
34_L38_G1.4560.95
111_D114_D1.4480.95
7_P58_K1.4360.94
131_I138_F1.4340.94
134_N221_T1.4280.94
112_P188_L1.4100.94
167_V174_A1.4060.94
119_P167_V1.4030.94
39_K106_A1.4000.94
173_T193_C1.3980.93
31_F67_F1.3940.93
135_I149_D1.3890.93
53_T83_F1.3780.93
118_D121_L1.3640.93
131_I156_L1.3570.92
53_T159_A1.3510.92
170_N236_D1.3400.92
123_L160_I1.3310.92
6_L169_R1.3300.92
192_L202_L1.3190.91
31_F35_Q1.3170.91
95_L102_L1.2870.90
163_A173_T1.2860.90
27_L65_A1.2770.90
69_K77_P1.2460.89
193_C200_P1.2400.89
161_E164_K1.2400.89
61_E85_K1.2350.88
12_Y22_I1.2270.88
172_K235_P1.2180.88
62_P169_R1.2100.87
150_R197_P1.2100.87
23_L26_Y1.2020.87
109_F170_N1.1980.87
11_D20_Y1.1950.87
162_L165_K1.1860.86
54_G106_A1.1840.86
102_L124_R1.1810.86
235_P239_W1.1760.86
5_A8_E1.1680.85
43_S46_G1.1630.85
166_R173_T1.1530.85
39_K104_E1.1410.84
126_N215_R1.1300.83
189_L228_N1.1270.83
40_I49_A1.1200.83
86_E122_E1.1080.82
188_L232_I1.0960.81
37_E106_A1.0950.81
85_K128_D1.0790.80
118_D174_A1.0780.80
60_Y169_R1.0760.80
61_E87_S1.0560.79
129_H137_R1.0550.79
202_L219_C1.0310.77
37_E205_V1.0290.77
47_K69_K1.0210.77
67_F80_F1.0090.76
193_C206_V1.0000.75
221_T228_N0.9940.75
136_D143_Q0.9900.74
11_D18_K0.9850.74
175_V229_A0.9820.74
151_N155_L0.9790.73
5_A63_I0.9760.73
156_L160_I0.9720.73
33_R36_E0.9620.72
1_L5_A0.9510.71
1_L8_E0.9480.71
49_A67_F0.9430.71
230_R239_W0.9380.70
134_N202_L0.9380.70
226_Y230_R0.9330.70
48_Y66_L0.9310.70
141_E195_T0.9310.70
56_V60_Y0.9280.69
29_Y231_L0.9260.69
43_S47_K0.9210.69
80_F141_E0.9190.69
53_T65_A0.9180.69
27_L43_S0.9020.67
21_P172_K0.8900.66
30_T106_A0.8880.66
107_N120_R0.8870.66
173_T238_D0.8850.66
110_D120_R0.8780.65
135_I138_F0.8780.65
184_G227_N0.8710.64
138_F169_R0.8680.64
177_Q184_G0.8660.64
96_L99_F0.8650.64
142_L195_T0.8640.64
54_G174_A0.8640.64
11_D16_S0.8590.63
194_L216_G0.8570.63
172_K238_D0.8560.63
27_L34_L0.8480.62
59_N127_Y0.8480.62
73_P76_Q0.8470.62
58_K198_S0.8430.62
56_V62_P0.8390.62
171_Y233_A0.8390.62
172_K233_A0.8380.61
184_G187_Q0.8370.61
63_I166_R0.8370.61
231_L237_S0.8290.61
188_L203_A0.8280.61
10_W23_L0.8280.61
207_E217_H0.8270.60
117_F186_I0.8260.60
10_W55_L0.8250.60
42_Y49_A0.8250.60
114_D208_K0.8240.60
55_L176_P0.8230.60
113_S161_E0.8210.60
206_V216_G0.8210.60
224_M228_N0.8160.59
46_G69_K0.8150.59
133_D168_R0.8150.59
223_D233_A0.8140.59
195_T201_D0.8120.59
13_S17_N0.8060.58
112_P115_L0.8050.58
209_T213_F0.8040.58
72_P76_Q0.8040.58
1_L4_L0.8030.58
107_N229_A0.8010.58
86_E165_K0.7960.57
117_F164_K0.7950.57
155_L192_L0.7930.57
188_L234_R0.7870.57
31_F105_R0.7850.56
123_L164_K0.7820.56
173_T188_L0.7790.56
40_I52_N0.7780.56
30_T52_N0.7750.55
30_T118_D0.7700.55
114_D219_C0.7670.55
57_T61_E0.7640.54
48_Y98_Y0.7590.54
156_L192_L0.7590.54
187_Q220_L0.7590.54
170_N179_Y0.7480.53
218_T226_Y0.7460.53
33_R186_I0.7450.53
166_R193_C0.7410.52
66_L84_C0.7390.52
28_E78_W0.7380.52
65_A80_F0.7340.51
212_G216_G0.7340.51
13_S16_S0.7340.51
12_Y15_P0.7330.51
160_I216_G0.7330.51
120_R163_A0.7290.51
186_I205_V0.7270.51
224_M227_N0.7250.51
119_P173_T0.7240.50
59_N128_D0.7230.50
177_Q220_L0.7220.50
194_L203_A0.7200.50
54_G108_Y0.7190.50
108_Y190_L0.7110.49
207_E215_R0.7070.49
13_S20_Y0.7050.49
178_Y194_L0.7030.48
111_D167_V0.7020.48
52_N169_R0.7010.48
235_P238_D0.6990.48
15_P19_P0.6970.48
50_C84_C0.6970.48
65_A186_I0.6950.48
51_F164_K0.6930.47
154_N158_G0.6890.47
63_I117_F0.6880.47
115_L165_K0.6860.47
16_S21_P0.6790.46
89_R126_N0.6780.46
31_F83_F0.6760.46
12_Y31_F0.6720.45
163_A167_V0.6650.45
4_L57_T0.6620.44
43_S48_Y0.6610.44
142_L155_L0.6600.44
50_C86_E0.6570.44
51_F203_A0.6560.44
48_Y59_N0.6540.44
11_D14_T0.6520.43
33_R106_A0.6510.43
58_K74_G0.6470.43
51_F139_P0.6470.43
79_F82_G0.6460.43
22_I194_L0.6440.43
160_I164_K0.6430.43
25_N227_N0.6400.42
25_N29_Y0.6390.42
36_E178_Y0.6360.42
16_S19_P0.6340.42
49_A146_D0.6330.42
34_L39_K0.6330.42
57_T148_N0.6320.42
91_L236_D0.6280.41
3_E7_P0.6280.41
160_I163_A0.6280.41
112_P202_L0.6260.41
229_A233_A0.6230.41
130_I137_R0.6210.40
41_V49_A0.6200.40
216_G237_S0.6190.40
124_R178_Y0.6170.40
32_R195_T0.6170.40
83_F190_L0.6170.40
84_C90_E0.6160.40
30_T33_R0.6160.40
80_F134_N0.6130.40
116_I172_K0.6110.40
126_N179_Y0.6110.40
173_T236_D0.6080.39
71_R74_G0.6080.39
137_R226_Y0.6050.39
85_K88_D0.6040.39
22_I48_Y0.6040.39
215_R236_D0.6020.39
131_I152_L0.6020.39
69_K76_Q0.6010.39
31_F211_S0.6010.39
21_P223_D0.5970.38
219_C224_M0.5970.38
220_L226_Y0.5940.38
92_G96_L0.5910.38
10_W26_Y0.5900.38
207_E216_G0.5900.38
15_P140_E0.5880.37
8_E23_L0.5870.37
105_R168_R0.5850.37
117_F198_S0.5850.37
30_T54_G0.5830.37
169_R232_I0.5820.37
119_P168_R0.5820.37
154_N157_E0.5820.37
84_C87_S0.5810.37
12_Y227_N0.5810.37
35_Q38_G0.5780.36
52_N226_Y0.5750.36
78_W166_R0.5750.36
73_P101_P0.5750.36
132_E186_I0.5740.36
89_R133_D0.5740.36
47_K71_R0.5730.36
9_D22_I0.5720.36
29_Y36_E0.5700.36
154_N162_L0.5690.36
191_P230_R0.5690.36
10_W230_R0.5680.36
64_Y171_Y0.5650.35
56_V173_T0.5640.35
141_E144_S0.5620.35
62_P105_R0.5620.35
74_G77_P0.5620.35
67_F103_P0.5600.35
41_V44_D0.5600.35
142_L149_D0.5570.35
63_I83_F0.5570.35
60_Y162_L0.5560.35
59_N164_K0.5490.34
169_R236_D0.5480.34
76_Q79_F0.5450.34
158_G219_C0.5440.33
42_Y78_W0.5440.33
17_N110_D0.5440.33
8_E26_Y0.5430.33
189_L201_D0.5420.33
188_L214_Y0.5420.33
20_Y160_I0.5410.33
37_E178_Y0.5400.33
66_L223_D0.5390.33
6_L105_R0.5390.33
169_R237_S0.5360.33
226_Y235_P0.5350.33
163_A222_L0.5340.33
60_Y168_R0.5300.32
14_T17_N0.5260.32
39_K108_Y0.5250.32
225_A228_N0.5230.32
225_A239_W0.5220.32
109_F234_R0.5220.32
177_Q205_V0.5210.31
32_R184_G0.5200.31
170_N231_L0.5180.31
103_P171_Y0.5160.31
30_T37_E0.5150.31
215_R234_R0.5150.31
76_Q226_Y0.5140.31
165_K231_L0.5140.31
9_D15_P0.5140.31
35_Q160_I0.5130.31
69_K83_F0.5120.31
18_K168_R0.5110.31
1_L10_W0.5090.30
1_L7_P0.5060.30
69_K147_R0.5060.30
31_F42_Y0.5050.30
26_Y55_L0.5040.30
20_Y60_Y0.5040.30
233_A236_D0.5040.30
34_L170_N0.5040.30
223_D235_P0.5000.30
30_T174_A0.5000.30
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4v06A 1 0.5941 9.2 0.962 Contact Map
1tohA 3 0.5732 8.7 0.963 Contact Map
1g4wR 1 0.2008 4 0.968 Contact Map
1bh9B 1 0.1464 3.1 0.97 Contact Map
1mlwA 1 0.4142 2.9 0.97 Contact Map
1oaoC 1 0.3598 2.3 0.972 Contact Map
4kgmA 5 0.5397 2.2 0.972 Contact Map
1phzA 2 0.5021 2 0.973 Contact Map
2y9yB 1 0.2301 1.9 0.973 Contact Map
2gr8A 3 0.251 1.9 0.973 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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