GREMLIN Database
MscS_TM - Mechanosensitive ion channel inner membrane domain 1
PFAM: PF12794 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 339 (337)
Sequences: 2015 (1576)
Seq/√Len: 85.8
META: 0.739

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
161_I215_L5.2201.00
32_V38_D4.7521.00
65_G82_G4.2611.00
214_A219_Y4.1761.00
25_L46_A4.1251.00
101_L107_L3.7311.00
21_L49_L3.1341.00
239_L315_F3.1271.00
38_D42_H3.1241.00
212_V216_A2.9881.00
131_L135_P2.9551.00
129_L170_F2.9291.00
176_R179_R2.9231.00
235_L318_G2.7601.00
109_E119_V2.5941.00
81_L138_F2.5831.00
17_L20_R2.5481.00
125_Q173_R2.5091.00
38_D248_R2.5001.00
176_R180_G2.4831.00
100_R103_R2.4801.00
172_Y200_Y2.4371.00
84_G142_L2.3821.00
161_I211_A2.3711.00
232_T323_W2.3671.00
35_V38_D2.3441.00
125_Q128_W2.3061.00
43_T111_H2.2471.00
132_V166_A2.2311.00
33_G38_D2.1531.00
105_G109_E2.1371.00
111_H248_R2.1361.00
258_A262_A2.1241.00
268_A272_A2.0371.00
125_Q170_F2.0311.00
96_E100_R2.0291.00
20_R23_R1.9791.00
24_R45_R1.9701.00
24_R27_R1.9601.00
40_F245_L1.9571.00
98_L237_L1.9471.00
46_A107_L1.9411.00
32_V37_T1.8921.00
151_A154_S1.8861.00
160_F164_L1.8841.00
21_L52_L1.8751.00
32_V42_H1.8541.00
39_S42_H1.8471.00
109_E116_E1.8111.00
29_A32_V1.7961.00
25_L50_T1.7861.00
38_D111_H1.7621.00
116_E120_R1.7581.00
208_L212_V1.7261.00
108_A114_W1.7031.00
102_C108_A1.6581.00
262_A266_R1.6491.00
219_Y223_L1.6341.00
160_F222_A1.6321.00
160_F214_A1.6251.00
56_P59_L1.6191.00
168_A204_V1.6181.00
81_L142_L1.6121.00
32_V35_V1.6011.00
259_L263_L1.5801.00
329_A339_W1.5741.00
24_R28_L1.5561.00
262_A265_R1.5511.00
88_L134_L1.5411.00
43_T248_R1.5391.00
170_F174_L1.5251.00
212_V215_L1.5211.00
18_R22_R1.5151.00
235_L322_I1.5000.99
128_W131_L1.4790.99
142_L156_G1.4740.99
121_R180_G1.4430.99
299_I302_Q1.4240.99
101_L108_A1.4130.99
92_L96_E1.4110.99
96_E99_R1.4040.99
320_W332_I1.3860.99
177_P180_G1.3850.99
6_L10_L1.3650.99
31_K37_T1.3560.99
46_A111_H1.3430.99
136_L162_L1.3400.99
94_V234_W1.3380.99
36_R259_L1.3320.99
11_L60_L1.3250.99
165_L169_V1.3230.99
130_I134_L1.3200.99
92_L130_I1.3070.99
161_I165_L1.3070.99
51_L93_L1.3070.99
25_L53_L1.3010.99
40_F44_L1.2840.98
36_R263_L1.2830.98
97_F101_L1.2790.98
69_S82_G1.2710.98
140_V163_A1.2710.98
153_D219_Y1.2670.98
267_E271_A1.2570.98
31_K42_H1.2550.98
62_A66_W1.2540.98
168_A208_L1.2510.98
144_E219_Y1.2420.98
168_A211_A1.2350.98
295_D298_A1.2240.98
32_V110_A1.2160.98
261_R299_I1.2130.98
264_E267_E1.2080.98
50_T106_G1.2020.98
65_G86_L1.1920.97
239_L311_L1.1900.97
168_A207_P1.1890.97
38_D43_T1.1860.97
279_E283_G1.1750.97
34_R37_T1.1720.97
294_I299_I1.1620.97
19_R23_R1.1620.97
26_E105_G1.1510.97
29_A111_H1.1370.97
260_R264_E1.1230.96
128_W169_V1.1210.96
132_V162_L1.1110.96
264_E268_A1.1090.96
62_A86_L1.1090.96
266_R269_A1.1060.96
35_V42_H1.1030.96
115_P118_A1.0980.96
292_P299_I1.0930.96
50_T108_A1.0910.96
276_E283_G1.0890.96
270_L274_E1.0870.96
98_L126_L1.0800.95
213_L221_T1.0790.95
138_F322_I1.0730.95
32_V111_H1.0730.95
250_L300_S1.0700.95
40_F249_W1.0660.95
260_R263_L1.0650.95
104_P119_V1.0590.95
261_R264_E1.0590.95
75_T78_S1.0540.95
25_L49_L1.0450.95
40_F107_L1.0410.94
210_L226_L1.0340.94
43_T107_L1.0330.94
248_R252_V1.0310.94
139_V159_A1.0270.94
251_L254_R1.0230.94
283_G287_P1.0180.94
246_V307_L1.0090.93
160_F228_R1.0060.93
261_R265_R1.0060.93
23_R27_R1.0040.93
119_V123_R1.0010.93
33_G37_T1.0010.93
284_E287_P0.9970.93
13_L17_L0.9950.93
300_S304_R0.9920.93
219_Y299_I0.9840.93
28_L49_L0.9830.92
106_G157_R0.9810.92
37_T42_H0.9810.92
136_L163_A0.9720.92
267_E270_L0.9690.92
4_F67_L0.9660.92
41_L45_R0.9650.92
239_L254_R0.9630.92
57_L134_L0.9610.92
147_P153_D0.9600.92
276_E281_E0.9590.92
206_L209_A0.9570.91
125_Q174_L0.9540.91
251_L255_R0.9520.91
84_G88_L0.9510.91
63_L67_L0.9510.91
247_L251_L0.9490.91
296_L299_I0.9450.91
136_L166_A0.9430.91
153_D221_T0.9400.91
283_G314_A0.9400.91
175_L203_L0.9340.90
44_L157_R0.9300.90
164_L211_A0.9280.90
310_L314_A0.9280.90
320_W328_P0.9260.90
38_D56_P0.9250.90
331_S334_D0.9190.90
207_P210_L0.9190.90
11_L15_L0.9190.90
52_L59_L0.9090.89
289_V292_P0.9070.89
4_F63_L0.9050.89
81_L95_L0.9020.89
312_L316_L0.9010.89
126_L170_F0.9000.89
8_L11_L0.8980.89
95_L99_R0.8950.88
186_L201_P0.8920.88
249_W258_A0.8900.88
156_G219_Y0.8870.88
302_Q305_R0.8870.88
297_E305_R0.8860.88
213_L260_R0.8840.88
254_R300_S0.8790.88
140_V159_A0.8780.88
224_Q324_S0.8710.87
143_A239_L0.8700.87
25_L218_Y0.8680.87
224_Q228_R0.8560.86
265_R293_E0.8550.86
123_R127_R0.8510.86
28_L32_V0.8510.86
117_A310_L0.8420.85
126_L131_L0.8420.85
293_E299_I0.8400.85
285_E288_P0.8390.85
157_R214_A0.8300.84
128_W135_P0.8300.84
101_L241_L0.8290.84
143_A151_A0.8270.84
158_L162_L0.8270.84
169_V173_R0.8270.84
51_L97_F0.8260.84
44_L252_V0.8240.84
195_L200_Y0.8200.84
25_L28_L0.8200.84
93_L96_E0.8190.84
197_Y200_Y0.8190.84
102_C114_W0.8100.83
220_Y256_R0.8100.83
160_F219_Y0.8090.83
317_V321_L0.8020.82
62_A89_A0.8010.82
61_L85_L0.8010.82
311_L314_A0.7990.82
303_S306_L0.7970.82
238_G242_L0.7950.82
20_R28_L0.7940.82
305_R333_L0.7930.82
137_V171_L0.7900.81
277_E282_E0.7880.81
11_L14_L0.7860.81
299_I310_L0.7860.81
39_S44_L0.7850.81
109_E120_R0.7850.81
138_F142_L0.7840.81
47_L245_L0.7800.81
269_A272_A0.7790.80
104_P113_R0.7780.80
198_L201_P0.7770.80
43_T112_F0.7750.80
304_R328_P0.7750.80
280_G283_G0.7750.80
7_L142_L0.7710.80
44_L249_W0.7700.80
105_G110_A0.7680.80
273_R278_A0.7640.79
250_L303_S0.7630.79
143_A155_L0.7620.79
54_A97_F0.7600.79
180_G186_L0.7590.79
46_A50_T0.7590.79
277_E284_E0.7580.79
228_R231_D0.7540.78
56_P60_L0.7540.78
133_L163_A0.7470.78
212_V279_E0.7460.78
228_R326_L0.7450.77
307_L320_W0.7440.77
206_L339_W0.7420.77
50_T97_F0.7420.77
25_L71_S0.7420.77
277_E283_G0.7410.77
48_L91_L0.7400.77
81_L84_G0.7350.76
229_L308_R0.7340.76
164_L226_L0.7320.76
48_L51_L0.7310.76
28_L137_V0.7270.76
194_R200_Y0.7270.76
135_P157_R0.7260.76
284_E288_P0.7260.76
283_G286_A0.7250.75
314_A324_S0.7250.75
7_L63_L0.7230.75
258_A296_L0.7190.75
221_T225_L0.7170.75
35_V254_R0.7150.74
9_L75_T0.7100.74
268_A271_A0.7090.74
266_R273_R0.7010.73
57_L92_L0.7010.73
153_D296_L0.6980.73
8_L12_L0.6960.72
320_W330_L0.6950.72
121_R176_R0.6930.72
38_D336_I0.6920.72
22_R59_L0.6900.72
193_W199_W0.6900.72
336_I339_W0.6880.72
118_A121_R0.6860.71
179_R183_A0.6840.71
141_A242_L0.6830.71
250_L253_A0.6830.71
50_T54_A0.6830.71
106_G114_W0.6790.71
28_L42_H0.6790.71
124_R162_L0.6780.71
51_L245_L0.6760.70
25_L228_R0.6760.70
45_R49_L0.6760.70
25_L103_R0.6750.70
32_V43_T0.6730.70
177_P200_Y0.6730.70
223_L226_L0.6720.70
144_E223_L0.6690.70
196_R199_W0.6690.70
302_Q315_F0.6680.69
57_L252_V0.6680.69
230_I234_W0.6670.69
234_W238_G0.6670.69
235_L315_F0.6660.69
279_E284_E0.6660.69
182_L221_T0.6650.69
170_F317_V0.6650.69
156_G159_A0.6620.69
144_E224_Q0.6620.69
17_L21_L0.6610.69
179_R214_A0.6610.69
52_L64_L0.6600.68
112_F179_R0.6560.68
114_W240_L0.6560.68
151_A156_G0.6540.68
261_R296_L0.6530.68
126_L168_A0.6530.68
311_L321_L0.6520.68
124_R216_A0.6480.67
326_L329_A0.6470.67
205_L241_L0.6460.67
140_V219_Y0.6440.67
221_T325_D0.6440.67
210_L222_A0.6430.66
21_L25_L0.6420.66
177_P181_L0.6400.66
6_L13_L0.6390.66
38_D259_L0.6390.66
143_A156_G0.6380.66
256_R297_E0.6380.66
82_G114_W0.6380.66
156_G223_L0.6340.65
95_L130_I0.6330.65
11_L63_L0.6320.65
309_T312_L0.6300.65
58_P126_L0.6290.65
307_L318_G0.6290.65
259_L262_A0.6280.65
147_P150_L0.6280.65
82_G218_Y0.6280.65
74_A82_G0.6260.64
53_L100_R0.6260.64
247_L304_R0.6250.64
121_R241_L0.6250.64
20_R24_R0.6250.64
232_T333_L0.6250.64
260_R309_T0.6230.64
107_L230_I0.6200.64
52_L105_G0.6190.64
255_R259_L0.6190.64
231_D321_L0.6190.64
194_R197_Y0.6180.63
317_V327_L0.6170.63
96_E130_I0.6150.63
270_L275_A0.6150.63
26_E30_E0.6150.63
151_A155_L0.6140.63
5_L19_R0.6120.63
226_L267_E0.6120.63
56_P243_Y0.6060.62
96_E103_R0.6050.62
84_G138_F0.6040.62
83_A87_A0.6020.61
23_R26_E0.6020.61
164_L210_L0.6010.61
92_L134_L0.6010.61
40_F112_F0.6000.61
80_A84_G0.6000.61
226_L247_L0.6000.61
15_L163_A0.5990.61
35_V252_V0.5940.60
9_L13_L0.5940.60
232_T296_L0.5930.60
10_L70_A0.5900.60
298_A330_L0.5900.60
28_L45_R0.5880.60
318_G321_L0.5870.59
33_G259_L0.5860.59
15_L18_R0.5850.59
272_A276_E0.5830.59
221_T268_A0.5820.59
299_I315_F0.5820.59
311_L317_V0.5800.59
270_L273_R0.5790.58
307_L328_P0.5780.58
35_V255_R0.5770.58
252_V298_A0.5770.58
89_A93_L0.5760.58
157_R236_L0.5760.58
206_L229_L0.5760.58
179_R185_L0.5750.58
50_T100_R0.5730.58
18_R244_A0.5720.58
9_L30_E0.5720.58
280_G288_P0.5680.57
57_L88_L0.5680.57
296_L339_W0.5670.57
76_P182_L0.5660.57
207_P228_R0.5660.57
255_R265_R0.5660.57
129_L215_L0.5610.56
206_L211_A0.5600.56
63_L257_L0.5590.56
318_G322_I0.5580.56
99_R130_I0.5570.56
154_S157_R0.5570.56
22_R53_L0.5560.55
180_G232_T0.5560.55
33_G48_L0.5560.55
322_I329_A0.5550.55
161_I220_Y0.5540.55
51_L233_L0.5480.54
256_R261_R0.5470.54
197_Y201_P0.5470.54
164_L230_I0.5460.54
176_R200_Y0.5450.54
273_R277_E0.5440.54
189_N193_W0.5420.54
51_L169_V0.5420.54
47_L97_F0.5410.53
29_A50_T0.5400.53
258_A266_R0.5400.53
23_R61_L0.5390.53
37_T221_T0.5380.53
243_Y308_R0.5380.53
117_A168_A0.5360.53
132_V135_P0.5360.53
58_P157_R0.5350.53
295_D334_D0.5340.52
27_R45_R0.5330.52
88_L138_F0.5330.52
30_E241_L0.5330.52
111_H252_V0.5320.52
113_R133_L0.5320.52
91_L133_L0.5320.52
220_Y224_Q0.5290.52
135_P138_F0.5290.52
137_V147_P0.5290.52
291_E295_D0.5280.52
102_C210_L0.5260.51
70_A127_R0.5250.51
39_S252_V0.5240.51
219_Y226_L0.5240.51
52_L246_V0.5240.51
160_F224_Q0.5220.51
202_L277_E0.5210.51
245_L249_W0.5210.51
274_E288_P0.5210.51
40_F157_R0.5200.51
289_V303_S0.5200.51
8_L29_A0.5180.50
184_S203_L0.5180.50
6_L20_R0.5160.50
152_S160_F0.5150.50
35_V259_L0.5140.50
65_G68_L0.5140.50
318_G328_P0.5130.50
315_F333_L0.5130.50
275_A280_G0.5130.50
162_L165_L0.5130.50
77_F138_F0.5120.50
207_P222_A0.5120.50
157_R160_F0.5120.50
325_D330_L0.5110.49
107_L164_L0.5110.49
85_L190_P0.5090.49
209_A237_L0.5070.49
24_R49_L0.5060.49
147_P221_T0.5060.49
54_A58_P0.5060.49
126_L171_L0.5050.49
63_L170_F0.5050.49
54_A315_F0.5040.48
258_A265_R0.5040.48
311_L330_L0.5030.48
114_W122_L0.5030.48
5_L16_W0.5030.48
161_I216_A0.5020.48
261_R295_D0.5020.48
164_L214_A0.5020.48
52_L60_L0.5020.48
11_L30_E0.5010.48
246_V253_A0.5000.48
318_G329_A0.5000.48
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3wdoA 1 0.7906 58.2 0.95 Contact Map
4m64A 1 0.708 46.1 0.954 Contact Map
4djkA 2 0.7198 25.8 0.96 Contact Map
4pypA 1 0.649 25.3 0.96 Contact Map
3pjsK 4 0.3982 22.1 0.961 Contact Map
4ltoA 3 0.3894 21.4 0.961 Contact Map
3effK 4 0.4071 16.7 0.963 Contact Map
4c7rA 3 0.1062 15 0.964 Contact Map
3w4tA 1 0.7021 13.3 0.965 Contact Map
4o6yA 2 0.5723 7.9 0.968 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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