GREMLIN Database
DUF3781 - Protein of unknown function (DUF3781)
PFAM: PF12636 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 72 (71)
Sequences: 1682 (1117)
Seq/√Len: 132.6
META: 0.906

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
45_R63_A4.7211.00
41_D52_V3.8751.00
35_K54_I2.2741.00
53_Y58_I2.1381.00
4_L37_K2.1341.00
12_L15_E2.1151.00
7_I33_K2.1131.00
11_E15_E2.0571.00
3_N66_Y1.9501.00
27_D30_E1.9401.00
44_I53_Y1.8841.00
29_V68_I1.8321.00
35_K55_D1.7951.00
36_I61_I1.7831.00
38_D41_D1.7161.00
14_I28_V1.6571.00
3_N6_K1.5631.00
30_E34_E1.5161.00
15_E22_N1.4271.00
31_W52_V1.3871.00
52_V61_I1.3561.00
31_W57_I1.3221.00
59_I71_A1.2671.00
44_I58_I1.2451.00
31_W35_K1.2291.00
45_R50_W1.2171.00
9_T29_V1.1990.99
46_K58_I1.1830.99
52_V57_I1.0950.99
41_D54_I1.0560.99
31_W50_W1.0260.98
10_T13_G1.0140.98
34_E42_N0.9830.98
50_W66_Y0.9820.98
14_I19_K0.9650.98
15_E18_K0.9160.97
21_L71_A0.9110.97
39_A42_N0.8890.96
18_K28_V0.8720.96
13_G20_N0.8540.95
48_K51_Y0.8540.95
34_E38_D0.8440.95
67_T70_T0.8370.95
60_T70_T0.8240.94
37_K42_N0.8080.93
33_K37_K0.8020.93
18_K24_D0.8000.93
43_I50_W0.7970.93
9_T13_G0.7910.93
22_N26_D0.7890.93
9_T14_I0.7840.92
10_T16_R0.7520.91
32_C50_W0.7490.91
51_Y60_T0.7450.90
2_E12_L0.7310.90
36_I41_D0.7270.89
42_N53_Y0.7220.89
7_I37_K0.7150.88
20_N67_T0.7100.88
11_E14_I0.6980.87
39_A43_I0.6910.87
10_T49_N0.6890.87
24_D34_E0.6860.86
29_V32_C0.6810.86
27_D68_I0.6790.86
44_I52_V0.6780.86
4_L33_K0.6750.85
2_E14_I0.6740.85
22_N42_N0.6630.84
9_T20_N0.6550.84
25_T28_V0.6400.82
34_E39_A0.6390.82
22_N44_I0.6360.82
49_N69_I0.6250.81
10_T48_K0.6250.81
8_H13_G0.6230.81
50_W55_D0.6210.80
30_E37_K0.6110.79
2_E53_Y0.6090.79
40_N53_Y0.6070.79
11_E30_E0.5970.78
40_N55_D0.5910.77
29_V59_I0.5750.75
2_E42_N0.5640.74
48_K70_T0.5610.73
13_G32_C0.5580.73
25_T42_N0.5560.73
36_I43_I0.5560.73
31_W41_D0.5550.73
67_T72_H0.5510.72
2_E44_I0.5490.72
35_K61_I0.5450.71
39_A53_Y0.5440.71
21_L32_C0.5410.71
13_G16_R0.5390.70
51_Y70_T0.5380.70
15_E19_K0.5260.69
45_R64_H0.5230.68
47_G51_Y0.5210.68
6_K66_Y0.5170.67
56_N65_S0.5150.67
30_E33_K0.5130.67
4_L26_D0.5100.66
2_E30_E0.5040.65
15_E34_E0.5020.65
8_H20_N0.5000.65
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1nktA 3 0.9583 35.5 0.885 Contact Map
4lctA 2 0.5278 21.4 0.897 Contact Map
4d0pA 1 0.7222 19.2 0.899 Contact Map
4cr2O 1 0.8889 10 0.911 Contact Map
4cr2R 1 0.9722 9.9 0.911 Contact Map
4d10A 1 0.9306 8.8 0.913 Contact Map
3txnA 1 0.5833 8 0.915 Contact Map
1tolA 1 0.6528 7.1 0.917 Contact Map
3j8bE 1 0.75 6.4 0.919 Contact Map
2heoA 1 0.7361 6.3 0.919 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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