GREMLIN Database
DUF3754 - Protein of unknown function (DUF3754)
PFAM: PF12576 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 136 (136)
Sequences: 1898 (1285)
Seq/√Len: 110.2
META: 0.862

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
130_N133_N4.5531.00
120_K124_Q3.0651.00
105_F109_L3.0341.00
7_L10_F2.8561.00
3_R9_R2.5211.00
33_K133_N2.3411.00
61_K114_T2.3191.00
13_E16_F2.2581.00
71_G75_L2.2011.00
58_D118_Y2.1671.00
132_D136_G2.1041.00
55_R58_D2.0921.00
3_R11_K2.0351.00
106_R110_G1.9141.00
21_R25_R1.8691.00
108_W112_Q1.8451.00
3_R10_F1.8401.00
110_G113_N1.8341.00
3_R8_V1.8281.00
14_A17_A1.7621.00
45_M50_T1.6951.00
46_L122_L1.5871.00
2_D9_R1.5821.00
39_P42_D1.5761.00
2_D10_F1.5641.00
19_K22_K1.5481.00
54_M133_N1.5441.00
18_E21_R1.5431.00
56_L60_L1.5311.00
132_D135_A1.5281.00
46_L134_N1.5081.00
6_L12_P1.4641.00
92_A95_G1.4561.00
17_A22_K1.4531.00
16_F20_K1.4451.00
21_R24_L1.4441.00
5_V34_L1.4441.00
15_Y19_K1.4241.00
35_F127_Y1.3891.00
33_K127_Y1.3741.00
5_V10_F1.3551.00
16_F21_R1.3351.00
13_E17_A1.3341.00
13_E18_E1.3231.00
3_R131_L1.3160.99
15_Y20_K1.3020.99
5_V8_V1.2290.99
18_E24_L1.2120.99
5_V11_K1.1750.99
17_A20_K1.1620.99
47_F60_L1.1510.99
90_L94_L1.1410.99
2_D7_L1.1290.98
14_A18_E1.1270.98
49_N59_K1.1250.98
83_V88_A1.1070.98
79_S83_V1.1020.98
12_P15_Y1.0940.98
15_Y18_E1.0800.98
14_A19_K1.0800.98
20_K24_L1.0640.98
7_L11_K1.0610.98
86_D90_L1.0370.97
30_V122_L1.0030.97
15_Y21_R1.0030.97
13_E20_K0.9990.97
127_Y133_N0.9910.96
60_L64_V0.9830.96
42_D100_L0.9800.96
62_L65_P0.9730.96
69_G99_A0.9690.96
52_V58_D0.9690.96
85_L88_A0.9680.96
65_P107_Q0.9620.96
8_V12_P0.9580.96
81_E84_A0.9550.96
8_V11_K0.9530.95
17_A21_R0.9510.95
66_A107_Q0.9500.95
84_A87_A0.9490.95
2_D5_V0.9450.95
9_R12_P0.9390.95
103_Y106_R0.9370.95
13_E19_K0.9220.95
62_L107_Q0.9110.94
17_A25_R0.9100.94
67_L70_G0.9030.94
56_L76_K0.8940.94
6_L10_F0.8910.94
65_P114_T0.8900.94
26_R29_S0.8890.93
33_K130_N0.8830.93
99_A121_Q0.8740.93
12_P17_A0.8710.93
11_K14_A0.8650.93
38_I55_R0.8600.92
91_L95_G0.8580.92
16_F25_R0.8540.92
111_Y118_Y0.8520.92
45_M122_L0.8470.92
3_R36_K0.8460.92
86_D89_S0.8430.92
62_L96_G0.8190.90
20_K80_G0.8160.90
19_K23_R0.8100.90
55_R69_G0.8060.90
86_D92_A0.8030.89
85_L89_S0.7940.89
7_L12_P0.7940.89
69_G122_L0.7810.88
3_R7_L0.7710.87
66_A111_Y0.7700.87
83_V87_A0.7680.87
83_V86_D0.7660.87
101_G104_G0.7620.87
7_L62_L0.7610.87
45_M125_N0.7570.87
44_E47_F0.7560.86
123_T129_K0.7540.86
70_G104_G0.7440.86
16_F19_K0.7380.85
49_N128_F0.7270.84
57_F61_K0.7190.84
22_K25_R0.7110.83
15_Y22_K0.7100.83
44_E76_K0.7030.82
32_L98_A0.6980.82
18_E23_R0.6910.81
1_Y20_K0.6820.80
1_Y8_V0.6800.80
36_K134_N0.6790.80
16_F24_L0.6760.80
110_G114_T0.6740.79
85_L95_G0.6700.79
5_V26_R0.6680.79
47_F59_K0.6660.79
27_P71_G0.6560.78
31_Y127_Y0.6470.77
113_N119_Q0.6430.76
14_A22_K0.6350.75
44_E49_N0.6320.75
34_L43_L0.6320.75
124_Q129_K0.6260.74
39_P50_T0.6250.74
111_Y114_T0.6230.74
89_S93_L0.6230.74
2_D8_V0.6210.74
56_L101_G0.6190.73
9_R30_V0.6170.73
119_Q133_N0.6170.73
37_N102_G0.6160.73
104_G108_W0.6140.73
14_A25_R0.6130.73
35_F45_M0.6120.73
14_A21_R0.6110.73
16_F22_K0.6080.72
61_K109_L0.6080.72
2_D13_E0.6080.72
112_Q119_Q0.6070.72
81_E85_L0.6010.71
116_L120_K0.6010.71
46_L70_G0.6000.71
39_P44_E0.6000.71
55_R63_G0.5970.71
109_L113_N0.5970.71
89_S98_A0.5910.70
41_A50_T0.5880.70
125_N135_A0.5880.70
36_K68_G0.5870.70
38_I46_L0.5870.70
123_T132_D0.5820.69
105_F113_N0.5820.69
88_A91_L0.5810.69
14_A20_K0.5790.68
47_F55_R0.5730.68
107_Q128_F0.5730.68
37_N131_L0.5690.67
42_D123_T0.5640.66
20_K25_R0.5610.66
64_V95_G0.5600.66
61_K103_Y0.5590.66
119_Q130_N0.5590.66
1_Y11_K0.5590.66
53_R60_L0.5560.65
121_Q129_K0.5490.64
58_D65_P0.5450.64
98_A106_R0.5450.64
54_M106_R0.5390.63
18_E22_K0.5350.62
72_P96_G0.5310.62
87_A93_L0.5310.62
17_A23_R0.5290.62
13_E21_R0.5280.61
56_L59_K0.5250.61
55_R77_L0.5230.61
95_G98_A0.5220.61
128_F134_N0.5220.61
107_Q124_Q0.5210.60
9_R23_R0.5180.60
130_N135_A0.5130.59
115_K126_L0.5090.59
18_E25_R0.5040.58
25_R32_L0.5020.58
127_Y136_G0.5000.57
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3uauA 1 0.4926 9.6 0.925 Contact Map
2rngA 1 0.3676 3.3 0.94 Contact Map
2e6xA 2 0.3529 3.1 0.941 Contact Map
4ovdA 2 0.0735 2.6 0.943 Contact Map
2mmuA 1 0.3603 2.5 0.943 Contact Map
1njrA 2 0.3676 2.1 0.946 Contact Map
4c2wC 1 0.3235 2.1 0.946 Contact Map
4il3A 1 0.3824 2.1 0.946 Contact Map
1t6t1 2 0.3456 1.9 0.947 Contact Map
3h7hA 1 0.4485 1.8 0.948 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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